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Biomedical subjects

K Fujii

Publications and source records attributed to K Fujii.

At least 901 records · Page 50Linked to original sources
PubMed1980

Cytochrome a-type terminal oxidase derived from Thiobacillus novellus. Molecular and enzymatic properties.

Cytochrome a-type terminal oxidase was purified from Thiobacillus novellus to an electrophoretically homogeneous state and some of its properties were studied. The enzyme shows absorption peaks at 428 and 602 nm in the oxidized form, and at 442 and 602 nm in the reduced form. The CO compound of the reduced enzyme shows peaks at 431 and 599 nm. The enzyme has 1 mol of haem a and 1 g-atom of copper per 55600 g and is composed of two kinds of subunit, of 32000 and 23000 daltons, respectively. The enzyme reacts rapidly with tuna, bonito and yeast cytochromes c as well as with T. novellus cytochrome c, while it reacts slowly with horse and cow cytochromes c. The reduction product of oxygen catalysed by the enzyme is water.

Amino Acids↗
PubMed1980

Prominent increase in the amount of a cytosol protein in transformed fibroblasts and ascites hepatoma cells.

Analysis of cytosol proteins by sodium dodecyl sulfate polyacrylamide slab gel electrophoresis revealed a prominent increase in the amount of a cytosol protein with molecular weight of 88 000 in transformed human adult, human embryo, mouse adult, and hamster embryo fibroblasts as compared with normal fibroblasts. The cytosol protein with Mr 88 000 is also increased in the cytosol of four kinds of rat ascites hepatoma cell as compared to normal and regenerating liver. The protein with Mr 88 000 exists as one of the major cytosol proteins in transformed fibroblasts hepatoma cells and HeLa cells, constituting 7--10% of total cytosol proteins. The data suggest that the cytosol protein with Mr 88 000 is associated with certain growth characteristics of cells.

Adult↗
PubMed1980

A genetic aspect of chromatin proteins of human brain tumors.

Electrophoretic analysis of chromatin proteins was performed on rat and human tissues. Species and organ specific patterns were demonstrated in them. Twenty cases of human brain tumours were also analyzed and each tumor had typical phoretic patterns of chromatin. Proteins obtained from histologically similar tumors display similar electrophoretic patterns. As to the ratio of chromatin protein to DNA, no apparent differences between benign and malignant human brain tumors were observed.

Animals↗
PubMed1980

Comparative study of tumorigenicity in mice administered transplacentally or neonatally with metabolites of tryptophan and its related compounds.

Intermediate metabolites of tryptophan, 3-hydroxy-L-kynurenine (3-OHKY), 3-hydroxyanthranilic acid (3-OHAA) and anthranilic acid (AA), and an enzyme inhibitor from 3-OHKY to 3-OHAA, isonicotinic acid hydrazide (INH) with or without 3-OHKY at the maximum tolerated dose were injected s.c. to infant CDF1 mice. AA and 3-OHAA were tested transplacentally for tumorigenicity. Animals treated were observed for 1 year. Hepatocellular adenoma was developed at the incidence of 21.7% in male mice administered with 3-OHKY and INH as compared with 5.6% incidence in control males, but no leukemia was induced. Incidences of lung (3.4--15.0%) and liver tumors (4--5%) in other groups treated at infant stage were comparable to that in controls (lung: 11.1%; liver: 5.6%). Other tumors were one angiogenic sarcoma in a female treated with 3-OHAA, and one granulosa cell tumor of ovary in female treated with INH. Transplacentally the 10.3% incidence of liver tumor in male offspring, whose mothers were treated with AA, was slightly higher than that in male control (5.6%). However, the incidences of tumor were apparently in a critical level in these experimental conditions.

3-Hydroxyanthranilic Acid↗
PubMed1980

Chromatography in presence of high concentrations of salts on columns of celluloses with and without ion exchange groups (hydrogen bond chromatography). Its application to purification of yeast enzymes.

1. All the water-soluble yeast enzymes tested, which were only partially precipitated at best in the presence of high concentration of salts such as ammonium sulfate or sodium formate, were adsorbed on a column of cellulose in the presence of the same concentrations of the salts, and the adsorbed enzymes were chromatographically eluted by decreasing the concentration of the salts. 2. Even in the presence of high concentration of the salts, the adsorbed enzymes were eluted by urea or by "hydroxy-rich" reagents such as sucrose. 3. Under the experimental conditions used, the salt concentrations required for elution of the adsorbed enzymes were lower with cellulose than with DEAE-cellulose, CM-cellulose, or P-cellulose, indicating that ion exchange groups, either cationic or anionic, affected the adsorption, although the ion exchange groups of DEAE-cellulose, CM-cellulose, and P-cellulose were weakly but definitely functional as ion exchangers even in the presence of high concentrations of the salts. 4. The principal attractive force between cellulose and the enzyme was deduced to be due to hydrogen bonding. 5. This hydrogen bond chromatography was applied for the purification of some yeast enzymes.

Alcohol Oxidoreductases↗
PubMed1980

Simple purification and properties of bovine pancreatic deoxyribonuclease I.

A simple purification method for pancreatic deoxyribonuclease I (DNase I) [EC 3.1.4.3] was developed by utilizing the technique of isoelectric focusing. The active protein was resolved in to at least four forms with different isoelectric points; the major components a, b, and c had isoelectric points at pH 5.2, 4.9, and 4.8, respectively, and that of the minor component d was at 4.7. The four components (a, b, c, and d) exhibited peaks similar to those observed by Salnikow et al. after phosphocellulose chromatography (A, B, C, and D). The four components were all free from RNase and protease activities and were very stable at 0-2 degrees C for at least four weeks. Further, each of the four peaks exhibited a single protein band after polyacrylamide electrophoresis. DNase I-a antibody was prepared; it was very specific for DNase I and precipitated with the other components (b, c, and d). The mode of endonucleolytic action of pancreatic DNase I-a purified from Worthington DP grade DNase I was investigated. The sedimentation patterns in neutral sucrose gradients of digest of circular duplex DNA in an early stage of hydrolysis suggested that DNase I produces single strand scissions in the initial attack in the presence of divalent metal ions.

Animals↗
PubMed1980

Quantitative analysis of trifluoroacetate in the urine and blood by isotachophoresis.

Trifluoroacetate (TFA), the major metabolite of halothane, was assayed by a newly developed isotachophoretic technique. This technique has several advantages over the presently used methods of analysis. It requires no special preparation of urine or blood samples. The sample volume is small (5--100 microliters) and the analysis time is short (30--90 min per sample). In addition, the method provides an analysis that is both qualitative and quantitative over a wide range of concentrations (from 2 nanomoles in 200 microliters to 200 nanomoles in 5 microliters). In this study, the assay was performed using HCl (0.001 M) in 1 per cent Triton X-100, titrated with beta-alanine to a pH value of 3.6--3.9 as the leading electrolyte and n-caproic acid (0.01 M) as the terminal electrolyte (50--100 muA migration current). Using this technique, daily urinary TFA excretion of seven patients was measured during halothane anesthesia and for 14 days postoperatively. The TFA values were highest on the second postoperative day (317--1,259 mg). The mean values of the urinary TFA excreted during the entire study (2,501 +/- 493 mg, mean +/- SEM) were much higher than those reported previously. The isotachophoretic technique provides a sensitive assay for future research into the biotransformation of halothane.

Biotransformation↗
PubMed1980

Microsurgical anatomy of the choroidal arteries. Fourth ventricle and cerebellopontine angles.

The microsurgical anatomy of the arteries supplying the choroid plexus in the fourth ventricle and cerebellopontine angles was examined under X3 to X20 magnification in brains from 25 adult cadavers. In the most common pattern, the branches of the anterior inferior cerebellar artery (AICA) supplied the portion of the choroid plexus in the cerebellopontine angle and adjacent part of the lateral recess of the fourth ventricle, and the posterior inferior cerebellar artery (PICA) supplied the choroid plexus in the roof and medial part of the lateral recess of the fourth ventricle. The superior cerebellar artery (SCA) gave rise to a choroidal branch in only one brain. The choroid plexus on each side of the midline was divided into a medial and a lateral segment. Each segment was considered two parts to facilitate the description of its blood supply. The medial segment, located in the roof of the fourth ventricle, was divided into a rostral or nodular part, and a caudal or tonsillar part. The lateral segment, located in the lateral recess of the fourth ventricle and cerebellopontine angle, was separated into a medial or peduncular part, and a lateral or floccular part. The AICA most commonly supplied all the floccular part and the lateral portion of the peduncular part, and the PICA most commonly supplied all of the tonsilar and nodular parts, and the medial portion of the peduncular part.

Arteries↗
PubMed1980

Induction of digestive tract tumors and leukemias in Donryu rats by administration of 1-amyl-1-nitrosourea in drinking water.

The effects of administration of 1-amyl-1-nitrosourea (ANU) at doses of 400, 200, 100 and 0 ppm in the drinking water to Donryu rats of both sexes were studied. Tumors developed in 166/205 (81%) rats that received ANU, as compared with 9/34 (27%) control rats. The incidence of digestive tract tumors was highest (76%), followed by hematopoietic tissue (11%) and liver (9%) tumors. In females, mammary tumors were also found. There appeared to be a dose-response relationship among the 3 treated groups as regards the incidence of tumors of the hematopoietic tissues and the average survival time.

Animals↗