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Biomedical subjects

K Fujii

Publications and source records attributed to K Fujii.

At least 829 records · Page 46Linked to original sources
PubMed1984

In vivo spin-trap study on anaerobic dehalogenation of halothane.

Radical formation in vivo by anaerobic dehalogenation of halothane is described in this paper. The radicals were stabilized by spin-trapping and assayed by electron spin resonance spectrometry. The radical adducts were formed by inhalation of halothane in vivo and increased with decrease in inspired oxygen concentration. Following administration of the spin-trap, the expired concentration of CF2CHCl and CF3CH2Cl which are the anaerobic metabolites of halothane decreased, but bilious trifluoroacetate which are aerobic did not change. These results strongly suggest that radical intermediates are produced in anaerobic dehalogenation of halothane to CF2CHCl and CF3CH2Cl.

Anaerobiosis↗
PubMed1984

Analytical isotachophoresis utilizing computer simulation. II. Assessment of optimum separation conditions for urinary trifluoroacetic acid metabolized from anaesthetic halothane.

Experimentally determined optimum separation conditions for a metabolite of anaesthetic halothane in urine, trifluoroacetic acid, were assessed by means of computer simulation of the isotachophoretic steady-state. The simulation confirmed that urinary acids and trifluoroacetic acid can be separated in the limited pH range of 3.5-3.7 buffered by beta-alanine, as far as the pH dependence of effective mobility is utilized. The separated fraction of the trifluoroacetic acid zone was identified by mass spectrometry. The simulated coefficient of the calibration curve agreed well with the observed value.

Computers↗
PubMed1984

Effects of sublingual isosorbide dinitrate on left ventricular performance during exercise in patients with myocardial infarction.

In order to investigate left ventricular performance during exercise in patients with myocardial infarction and evaluate the effects of sublingual isosorbide dinitrate (ISDN) on left ventricular performance, we performed a symptom-limited multigraded exercise test using a bicycle ergometer in supine position. Thirty-seven patients with myocardial infarction were evaluated in order to clarify the hemodynamic responses to exercise with and without sublingual ISDN. Patients were subdivided into 3 groups according to the level of pulmonary capillary pressure (PCP) and cardiac index (CI) at peak exercise as follows: Group I (14 patients); PCP less than 18 mmHg, CI greater than or equal to 5.0 or CI less than 5.0 L/min/m2, Group II (11 patients); PCP greater than or equal to 18 mmHg, CI greater than or equal to 5.0 L/min/m2, Group III (12 patients); PCP greater than or equal to 18 mmHg, CI less than 5.0 L/min/m2. Exercise capacity without ISDN (control study) was correlated with left ventricular performance during exercise. Although left ventricular performance in patients who complained of dyspnea or chest pain at peak exercise was worse than those who complained of leg fatigue, we could not predict hemodynamics during exercise from the level of hemodynamic parameters at rest in each patient. Determinant factors of left ventricular performance during exercise were age, previous history of myocardial infarction, the severity of coronary artery lesion and the extent of left ventricular wall motion abnormality which was estimated by left ventriculogram as an index of infarct size. After sublingual ISDN (ISDN study), exercise capacity was improved. No patient terminated exercise because of chest pain and only one did because of dyspnea.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗
PubMed1984

Immediate prognosis in acute myocardial infarction: serial changes in immediate mortality rate and cause of death.

In order to evaluate the effect of coronary care on the immediate mortality rate of acute myocardial infarction and to clarify the problems in coronary care, we attempted to investigate the immediate mortality rate and causes of deaths in 492 patients with acute myocardial infarction who had admitted to the hospital within 24 hours after the onset of infarct from January, 1972 to December, 1981. Three hundred and seventy-nine were men and 113 were women, and their mean age was 61.8 years ranging from 28 to 91 years. One hundred and twenty-seven patients had a previous history of myocardial infarction and 365 patients had none. One hundred and ninety patients of these 365 patients without a previous infarction had anterior infarction, 152 patients inferior infarction and 23 patients subendocardial infarction. All patients were subdivided into four stages according to the time of the onset of infarct: Stage 1 (29 patients); 1972-1975, Stage 2 (101 patients); 1976-1977, Stage 3 (148 patients); 1978-1979 and Stage 4 (214 patients); 1980-1981. No significant differences in age, sex and infarct site among the four periods were found. Time interval between the onset of infarct and admission shortened serially and the ratio of reinfarction to initial infarction increased. Immediate mortality rate (within four weeks after the onset of infarct) was 20.5% in all patients. Immediate mortality rate in Stage 1 was 24.1%, 26.7% in Stage 2, 23.0% in Stage 3 and 15.4% in Stage 4, respectively.(ABSTRACT TRUNCATED AT 250 WORDS)

Aged↗
PubMed1984

[Non-invasive estimation of transmitral pressure gradient and mitral valve area in mitral stenosis by an ultrasonic pulsed Doppler technique].

We attempted to estimate transmitral pressure gradient and mitral valve area (MVA) noninvasively in mitral stenosis (MS) by a bi-directional pulsed Doppler flowmeter combined with an electronic two-dimensional echocardiograph. Eleven patients with MS in sinus rhythm were studied by cardiac catheterization. Fifteen healthy subjects (H) served as normal control. The pulsed Doppler flowmeter operated with a carrier frequency of 2.5 MHz, a pulse repetition rate of either 5 KHz or 10 KHz and a sample volume of 1 X 3 X 3 mm. The velocity of transmitral central flow was measured by this system, monitoring audible Doppler sounds and cardiac images which depict the anatomic location of the sampling site. The Doppler signal was analyzed by a sound spectrograph. In estimating the transmitral pressure gradient and MVA, we employed a Doppler parameter (half time) defined as the time for instantaneous maximal blood flow velocity to reduce to one-half from its rapid inflow peak, which is independent of the angle between the ultrasonic beam and blood flow. Transmitral pressure gradient (delta P100) was measured as the pressure gradient between either left atrial or pulmonary capillary pressure and left ventricular pressure at the point after 100 msec from the nadir of left ventricular early diastolic pressure [( LA or PC--LVDP]100). MVA was obtained using a Gorlin's formula. The transmitral blood flow velocity in both MS and healthy groups revealed a narrow frequency band pattern with two peaks, R and A, in diastole. The former peak occurred during rapid inflow phase and the latter following atrial contraction. In the healthy group, the descent rate of R wave was increased than that in the MS group. The square root of the pressure gradient also reduced linearly with transmitral flow velocity in the MS group. Thus in the MS group, the transmitral velocity was directly proportional to the square root of the pressure gradient as described by a Bernoulli theorem, and the half time was proportional to the transmitral velocity. The square of the half time (delta t2) was highly correlated with delta P100 (r = 0.97), and the inverse of the half time (delta t-1) was correlated with MVA (r = 0.76). There was no significant correlation between delta P100 and diastolic descent rate of anterior mitral leaflet (DDR). The present study indicates that the half time is useful in estimating transmitral pressure gradient and MVA in mitral stenosis.

Adult↗
PubMed1984

Japanese adult siblings with Tangier disease and statistical analysis of reported cases.

A 28 year old Japanese female was admitted to our university hospital because of extremely low plasma cholesterol levels (50mg/dl). On physical examination, tonsillar remnants and several flat patches of yellow-orange lymphoid tissues in the pharynx were found. Her plasma HDL was as low as 2.4 mg/dl. Laparoscopic findings showed typical yellow patches on the surface of the liver, and liver biospy specimens contained cholesterol esters demonstrated by the Schultz reaction. Colonofiberscopy showed orange-brown spots present throughout the rectum. Histologically, "foam cells" were recognized in the lymphoid tissue of the pharynx and rectal mucosa. Her sister, 26 years old, also revealed yellow-orange tonsils, low plasma cholesterol levels (39mg/dl) and low plasma HDL levels (8mg/dl), and was also diagnosed as Tangier disease. From a statistical analysis of the reported cases, it is noteworthy that yellow-orange tonsils, which were said to be the hallmark of Tangier disease, were not found in 20% of the patients. However, it is important that we could find rectal mucosa studded with orange-brown spots in all of the cases examined, and it contained "foam cells" histologically. It was concluded that when we suspect Tangier disease on account of hypocholesterolemia, we must first do a proctoscopic examination.

Adult↗
PubMed1984

[Primary hyperparathyroidism in cases of urinary stone].

During the 5-year period from October, 1978 to September, 1983, primary hyperparathyroidism has been found at our clinic in 16 out of 395 cases of urinary stones (4.1%) and in 12.6% of the recurrent or multiple stone-forming patients. Plasma-ionized calcium level and rapid rapid calcium load test were most valuable in the diagnosis of primary hyperparathyroidism. It has been proved that neck surgery for primary hyperparathyroidism prevents recurrence of urinary tract stones.

Adolescent↗
PubMed1983

Inter- and intramolecular stacking interaction between indole and adeninium rings.

Crystals of 1,9-dimethyladeninium-indole-3-acetate (1:1) complex (I) and 9-(3-indol-3-ylpropyl)-1-methyladeninium iodide (II), an inter- or intramolecular model for the stacking interaction between the tryptophanyl residue and the methylated (or protonated) adenine base, were subjected to X-ray analyses. Nearly parallel stacking and interplanar spacing near to 3.4 A were observed between the indole and adeninium rings of both crystals. In particular, one of the two stacking pairs formed in I showed the existence of a partial charge-transfer interaction in their ground states. On the basis of the molecular orbital consideration, the mutual orientation between these stacked aromatic rings is considerably governed by the orbital interaction between the highest occupied molecular orbital of the indole ring and the lowest unoccupied one of the adeninium ring. The ring stacking observed in II was stabilized by the strong coupled dipole-dipole interaction. Absorption, fluorescence, and proton nuclear magnetic resonance spectra indicated the existence of a stacking interaction in the aqueous solutions of I and II, as well as in their crystalline states. The biological implication for the observed stacking interactions has been discussed.

Adenine↗
PubMed1983

Abnormal collagen synthesis in skeletal muscle of dystrophic chicken.

Specific molecular properties of skeletal muscle collagens from normal and dystrophic chickens have been compared. When dystrophy develops in skeletal muscle tissue there was an increase in the amount of total collagen and an increased proportion of Type III collagen in the tissue. The results from the cross-link study as well as the analysis of the solubility of collagen showed that skeletal muscle of dystrophic chicken produces more immature collagen fibers compared to normal chicken. These findings strongly indicate an important role of collagen in the pathogenesis of the extensive connective tissue prolipheration characteristic of muscular dystrophies.

Animals↗