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Biomedical subjects

K Edwards

Publications and source records attributed to K Edwards.

At least 37 records · Page 2Linked to original sources
PubMed1987

Bone and joint infections in children.

The current approach to the diagnosis and treatment of bone and joint infections in children is presented. Guidelines for the surgical and medical regimen of each are outlined.

Adolescent↗
PubMed1986

Childhood Kaposi's sarcoma in Papua New Guinea.

In the country of Papua New Guinea, with its diverse population of nearly 3 million people, some disease patterns have a strange similarity to those seen in tropical Africa. Kaposi's sarcoma is a condition which is commonly seen in adult males; childhood Kaposi's sarcoma is relatively uncommon. Six cases, all males, aged from 6 months to 12 years have been detected since 1978. The primary organ involvement included skin in four cases, oral cavity in one case and lymph node in one case. The varied clinico-pathological features are described. This is the first report of such cases from Papua New Guinea.

Child, Preschool↗
PubMed1985

Monitoring urinary bladder temperature.

We evaluated bladder, rectal, and esophageal temperatures compared with nasopharyngeal temperatures in 12 patients who underwent cardiac surgery that required CPB. Changes in bladder temperature lagged behind those of esophageal and nasopharyngeal temperature during rapid cooling and rewarming. Changes in rectal temperature were the slowest. Bladder and rectal temperatures were similar after surgery. Monitoring bladder temperature represents the core temperature in a steady state.

Adult↗
PubMed1979

Relationship between protein synthesis and secretion in liver cells and the state of the adenine nucleotide system.

Adenine nucleotide levels could be precisely and reproducibly adjusted in liver cell suspensions by partially depleting the ATP pool with D-fructose or glycerol. Thus, it was possible to quantitatively correlate rates of protein synthesis and secretion with intracellular levels of ATP and with derived parameters, such as the adenylate energy charge. Half the maximum rate of incorporation of leucine into protein was observed at an energy charge of 0.80, a ratio of ATP to ADP of 2.6, and an ATP level of 1.05 mumol per g of wet cells. Proteins were secreted with half the maximum rate at an energy charge of 0.85, a ratio of ATP to ADP of 3.1 and an ATP concentration of 1.1 mumol per g of wet cells. Protein secretion did not depend on continued synthesis. Inhibitors of oxidative phosphorylation inhibited protein secretion in addition to protein synthesis, in contrast to observations by other authors on liver slices.

Adenine Nucleotides↗
PubMed1977

Group B streptococcal meningitis: delayed response to treatment.

Four infants with group B streptococcal meningitis had a delayed response to antibiotic therapy. Resolution of cerebrospinal fluid infection and/or pleocytosis occurred only after prolonged and intensive antimicrobial management. In this respect, these infants were similar to infants with gram-negative enteric meningitis. It is suggested that infants with group B streptococcal meningitis be closely monitored to insure adequate response to therapy.

Chloramphenicol↗
PubMed1977

Danger of sunburn following vaccination.

The complications of smallpox vaccination are reviewed. A case of disseminated vaccinia is presented. It is suggested that patients with sunburn may be susceptible to the complications of smallpox vaccination.

Child, Preschool↗
PubMed1976

Studies on the organisation of the chicken genome and its expression during myogenesis in vitro.

DNA from the chicken genome was analysed both by isopycnic centrifugation in cesium salt density gradients and by reassociation analysis using hydroxyapatite (HAP) chromatography. Centrifugation in neutral CsCl revealed a single non-Gaussian band skewed toward the heavy side, but no discrete satellite components. In heavy metal (Ag+ or Hg++)-Cs2SO4 gradients, 4-8 satellite bands were revealed, comprising 5-9% of the total DNA. Purification of the satellites and recentrifugation in neutral CsCl demonstrated that 80-90% of this DNA would band in the shoulder, with the remainder in the main band. These satellites can account at most for 30% of the heavy shoulder DNA, thus most of the heavy shoulder DNA must be of lower repetition frequencies. Reassociation analyses of chicken DNA demonstrated that the complexity of the non-repetitive DNA is 9.49 X 10(8) nucleotide pairs, equivalent to about 90% of the haploid genome. Repetitive DNA comprises only 8-10% of the genome and has the following composition, relative to total DNA: 3.7% intermediate repetitive, 1.9% highly repetitive, and 3.9% "zero-time binding" DNA. This unusually low repetitive DNA content may be related to the small genome size of chickens, relative to other vertebrates, and to the presence of many microchromosomes in the chicken karyotype. Total cell RNA extracted from perfusion myoblasts, post-fusion myotubes, and myoblasts grown in BrdU was incubated in large excess with 3H-TdR labelled non-repetitive DNA and the resulting hybrids assayed by HAP chromatography. The amount of non-repetitive DNA represented in the RNA was found to increase from 7-8% in the myoblast stage to 10-11% in myotubes. An even smaller proportion, about 5%, is represented in the RNA of myoblasts prevented from differentiating by growth in BrdU.

Animals↗
PubMed1976

Synthesis of albumin via a precursor protein in cell suspensions from rat liver.

The mechanism of the biosynthesis of albumin was studied in cell suspensions from rat liver. The cells were prepared by continuous perfusion of the liver in situ with 0.05% collagenase and 0.10% hyaluronidase and incubated under conditions optimized for the incorporation of amino acids into protein. Seven minutes after starting the incubation L-[1-14C]leucine was added, followed after 25 min by a 15 or 30-min chase with an 830-fold excess of non-radioactive L-leucine. Total protein, an albumin-like protein, and albumin were isolated from samples withdrawn immediately of total protein was found to remain constant after addition of the non-radioactive L-leucine, whereas that of the albumin-like protein decreased and that of albumin increased with incubation time. The increase in albumin radioactivity accounted for the decrease in radioactivity of the albumin-like protein, suggesting that the latter is a precursor of albumin. The precursor protein differed from albumin by an oligopeptide extension at the N-terminal end.

Albumins↗