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Biomedical subjects

K Dietzmann

Publications and source records attributed to K Dietzmann.

At least 73 records · Page 4Linked to original sources
PubMed1990

[Nerve fiber regeneration in preformed tissue spaces].

Described in this paper is a new artificial chamber model to enable in vivo investigation of nerve fiber regeneration. Transection of the sciatic nerve of a rat was followed by analysis of axonal regeneration and revascularisation in the tissue chamber. Vascularisation of the endoneurium via the newly formed epineurium and perineurium was almost physiological in the cavity formed. The latter was found to be suitable for application of drugs and neurotrophic substances.

Animals↗
PubMed1989

[Lipid and lectin histochemical studies of neurolipidosis].

Congenital glycolipidoses of the nervous system cause accumulation of various storage substances in cells. Methods of lipid and lectin histochemistry were used in an attempt for classification by known groups of diseases, on the basis of stored lipids. While conventional methods of lipid histochemistry enabled classification of accumulated substances only by lipid classes, lectin approaches provided additional information on sugar bonding points in cases of glycosphingolipidoses. For example, HPR-labelled and FITC-labelled WGA was positively recorded from neurons in cases of GM 2-gangliosidoses and mucopolysaccharidoses, type HURLER. Particular interest was aroused by RCA-I and PNA-positive reactions in glial cells of globoid cell leucodystrophy, since no histochemical method had been available in the past for diagnosis of this storage disease. Hence, the findings recorded by ALROY et al. (1986) from Twitcher mice, an animal model of galactosylceramidosis, have been confirmed by human material.

G(M2) Ganglioside↗
PubMed1989

[The morphology of coxsackie B virus infections of the nervous system].

Neuropathological studies were conducted into 20 virologically secured Coxsackie-B virus infections of infants and adults, with the nervous system being involved. Inflammatory processes affect both gray and white cerebral regions as well as the peripheral nervous system, and in adulthood they may take the form of severe necrotising encephalitis. Necroses of that kind may be recordable from various regions of the central nervous system. Notwithstanding tempestuous progress in the development of monoclonal antibody kits, culturing of Coxsackie viruses will continue to be of substantive importance to diagnosis, because of the small size of pathogens.

Adolescent↗
PubMed1989

[Innervation anomalies].

The deviation from anatomically described innervation can often occur. It is mainly described on muscles of the limbs. Constant attention should give to this fact during the process of diagnosis of peripheral nerve lesions. Electrophysiological methods (EMG, ENG with blockade of nerve, collision technique and simultaneous lead) can help to detect this anomalies. Double innervation, abnormal innervation and communications among nerves are causes of anomalies of innervation. The fact of communications of fibers among nerves is better called "nerve communication" than "nerve anastomosis". It is vague whether this anomalies of innervation are embryological malformations or reactions of adaptation after different diseases.

Arm↗
PubMed1987

Mitochondrial membrane potential in living cells: evidence from studies with rhodamine 6 G as fluorescent probe.

Rhodamine 6 G as a cationic permeant fluorophore is demonstrated to be selectively accumulated by mitochondria of living pancreatic acinar cells from guinea pigs. The accumulation of the fluorescent dye was studied by means of the application of electron transport inhibitors in the respiratory chain, ionophores and some hydrogen donors. Besides, some unspecific staining processes were excluded. Using this technique, it becomes possible to estimate the energy state of living cells under various conditions of energy supply and demand.

Animals↗
PubMed1987

[Value of muscle biopsy in the assessment of the prognosis of brachial plexus paralysis].

Since Plexus brachialis lesions mainly occur in young persons, their socio-medical significance is undisputed. While the diagnostic rules are largely standardised, the time and the kind of the surgical intervention is disputed. The enzymic-histochemical examination of the denervated muscles of nine patients confirm the trend towards an intervention as early as possible. After only a few days serious changes of the mosaic structure of the muscles with a great number of histopathological particularities had occurred. After half a year, the muscles already showed considerable fibrous changes, so that re-innervation is highly restricted. The enzymic-histochemical and clinical findings largely correlate.

Adult↗
PubMed1986

Morphological and biochemical characterization of pancreatic acinar cells from guinea pig.

For elucidation of pathogenesis of acute pancreatitis an evaluation of the "intactness of cellular metabolism" of the acinar cells is an important question. To this end single acinar cells were isolated from guinea pig pancreata and studied at morphological and biochemical levels. The presented procedure generally results in suspensions with more than 85 percent intact exocrine cells which maintain their typical arrangement of the zymogen granules in the apical region during isolation procedure. The cellular content of amylase, lipase, and ALAT was determined. The measurement of parameters of cellular energy metabolism was regarded as essential for determination of functional intactness of the pancreocytes. The oxygraphically measured span between unstimulated respiration and that after maximal stimulation by uncoupling was used to characterize the load capacity of cellular energy metabolism.

Amylases↗
PubMed1986

[Vital fluorochrome staining of isolated pancreatic acinar cells for the characterization of cell-structural changes].

Rhodamine 6 G as a cationic fluorophore is demonstrated to be selectively accumulated by mitochondria of living pancreatic acinar cells (cell isolation see Spormann et al. [1986]. The accumulation of rhodamine was studied under using of electron transport inhibitors, ionophores and some hydrogen donors. The application of DNP as wellknown protonophore resulted in a rapid dissipation of any fluorescent signals, whereas application of sodium succinate, hyperosmolaric exhibited a remarkable increase of fluorescence intensity. Using this technique it is possible to estimate the energy state of living cells under various conditions of energy supply and demand.

Animals↗
PubMed1986

[Neuromyotonic syndrome].

A syndrome with constant rest activity was observed in the EMG of a 28-years-old female patient. An H-reflex was detected for normal nerve conduction velocity. Hypomagnesemia was striking among the laboratory values. Bioptic investigation yielded centralisation of nuclei, variations in the diameter of muscle fibres and a predominance of type I fibres. The findings and the response to Finlepsin point to neuromyotonic syndrome.

Adult↗
PubMed1985

[Isolated exocrine pancreas cells--a model for the study of the pathogenesis of experimental acute pancreatitis].

For elucidation of the very initial phases of pathogenesis of acute pancreatitis an evaluation of the cellular metabolism may be an important question. Therefore we isolated acinar cells from guinea pig and studied them at morphological and biochemical levels. Generally, the cells maintained their typical arrangement of the enzyme granules in the apical region during isolation procedure. The measurement of the parameters of cellular energy metabolism was regarded as essential for determination of functional intactness of the pancreocytes. To this end oxygraphic and fluorescence microscopic methods were applied. The results show that the hydrogen supply of isolated acinar cells can be remarkably improved by addition of glucose or 80 mM succinate which was sensitively recorded by fluorescence microscopic measurement of rhodamine accumulation. The difference between maximal fluorescence and minimal one at excess dinitrophenol can be used as measure for evaluation of the energy state of isolated pancreatic acinar cells.

Acute Disease↗
PubMed1985

[Morphologic and histochemical studies of the development of chloroquine-induced storage disease in the semilunar ganglion of the rat].

Intralysosomal storage of polar lipids can be induced in rats by treatment with Chloroquine, which belongs to cationic amphiphilic compounds. The drug accumulates in lysosomes of cells of the peripheral nervous system and interferes with the catabolism of polar lipids. As a consequence the lysosomes are transformed into bodies which are characterized by an internal lamellated pattern. The lipids stored in the neuron cells are phospholipids. The purpose of the present study was to check up the chronological development of the structural and lipid histochemical alterations within neuron cells. A significant enlargement of perikarya was observed after treatment of 94 d. A moderate increase of lysosomes and phospholipids was noticed after 82 d. Some typical foam-cells were seen after a period of 250 d. Moreover, a special mitochondrial alteration was observed, which could express close correlations between phospholipid metabolism and damaged parts of mitochondria.

Animals↗
PubMed1984

Morphometric-interferometric investigations of neurons in the medial thalamic nucleus of newborn rats following maternal treatment with ethanol.

The nucleus and cell size, as well as the dry weight and protein contents of neurons in the medial thalamic nucleus of newborn rats, exposed to ethanol in utero, were examined on the first day of postnatal life. Comparison was made with a control group of animals, not exposed to ethanol. No significant changes in the protein content and dry weight values were observed. The perikarya of neurons from animals exposed to ethanol were significantly larger than those of the controls. The findings indicate that the nucleus and perikaryon are affected differently by alcohol, following intrauterine exposure.

Animals↗
PubMed1984

[Histochemical-morphometric studies of skeletal muscles in advanced arterial circulation disorders of the extremities].

Biopsies of M. gastrocnemius and Nervus suralis of 12 patients with advanced disturbances of circulation were examined by means of morphometrical and histochemical methods. The histological picture of a neurogenic atrophy of the muscle fibres with partially strong hypertrophy of the slow-twitch fibres was found. The main reaction patterns and the pathogenetic mechanisms are discussed. Alterations in the ratio of type I and type II fibres are described. Fiber type disproportions, i.e. more than 75% type I fibres, may suggest that in hypoxia the type II fibres are more intensely damaged than type I fibres. There are correlations between pathological fibre distribution and the success of vasosurgical treatment.

Adenosine Triphosphatases↗
PubMed1983

Experimental hypo/hyperthyroidism in rats and the perinatal development of the hypothalamo-neurohypophysial system in comparison with the thyroid gland state and external features.

Neurophysin was detected immunohistochemically in the hypothalamo-neurohypophysial system of Wistar rats not before fetal day 18. Formerly, neurophysin was identified on day 16 of intrauterine life using another breeding stock of Wistar rats, but the same immunohistochemical reagents. In pregnant rats, experimentally induced hypo/hyperthyroidism beginning with day 13 of gestation failed to show any evident influence on the first appearance of immunohistochemically detectable neurophysin during the fetal development. Otherwise, significant effects on fetal body growth and other external features as well as the fetal thyroid state and histochemically demonstrable thyroid peroxidase activity were shown. The influence of thiamazol on the fetal thyroid peroxidase points out a primary effect and indicates the permeability of the placenta to this antithyroid drug.

Animals↗
PubMed1983

[Histochemical studies on striated muscle of ischemic and angiosurgically reperfused lower extremities].

There have been reports on an increased oxidative capacity in muscle tissue from the diseased legs of patients with intermittent claudication. In biopsy from specimens the gastrocnemius muscle of 25 patients the effect of arterial reconstructive surgery were studied, using fresh frozen cryostate sections and histochemical reactions for some oxidoreductases. The present study describes additional histochemical changes in the leg muscles in patients suffering from arterial insufficiency. The changes observed are correlated to the clinical severity of the disease.

Arterial Occlusive Diseases↗
PubMed1982

[A Lead-containing lipomelanosis of the human nucleus dentatus cerebelli].

A case of a rare pigmentation of nucleus dentatus and stratum granulare cerebelli was investigated with microscopical and histochemical methods such as atom absorption and neutron activation analysis. The histochemical results of the analysis suggest that the pigment may belong to the group of melanin. The prepigments are rich in chromolipoids. The concentration of copper, zinc, iron and lead is increased. The larger content of lead has to be regarded as the very pathological symptom in the human cerebellum.

Aged↗