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Biomedical subjects

K Balogh

Publications and source records attributed to K Balogh.

At least 91 records · Page 5Linked to original sources
PubMed1975

Studies on cartilage formation. XX. Histochemical investigation of some enzymes of glycogen metabolsim in regenerative articular surfaces.

In 28 dogs the distal articular cartilage of the femur was removed and the regenerating articular surface on the 70th postoperative day was studied histochemically for hexokinase, glucose-6-phosphatase, phosphohexose-isomerase, fructose-1, 6-diphosphatase, aldolase, glyceraldehyde-3-phosphate dehydrogenase, lactate dehydrogenase, lactate dehydrogenase isoenzymes, phosphoglucomutase, phosphorylase, glycogen synthetase, UDP--glucose dehydrogenase, and UDP-glucuronic acid-4-epimerase. The articular surface consisted of fibrous tissue and of cartilage islets. The latter contained cells differentiating into cartilage and young chondrocytes. The glycolytic enzymes reacted positively in the regenerative articular surface. Enzyme activities were higher in the cells (particularly the chondroblasts and young chondrocytes) of the cartilage islets than in the connective tissue. In the cells differentiations into cartilage, beside the LDH isoenzymes characteristic of glycolysis, a significant LDH1 and LDH2 activity was observed. At the same site the presence of fructose-1, 6-diphosphatase-activity could be assumed, but there was no glucose-6-phosphatase activity. Glycogen synthesis proceeded in the cells of the cartilage islets and UDP-glucuronic acid-4-epimerase activity was observed in the differentiated cells. UDP-glucose dehydrogenase activity was positive in every section of the articular surface.

Animals↗
PubMed1973

Histochemical characteristics of parafollicular cells and medullary thyroid carcinoma.

Normal canine parafollicular cells share a common set of histochemical characteristics, which include masked metachromasia and argyrophilia, with human as well as rat medullary thyroid carcinomas. Masked metachromasia, a property of polypeptide hormone-producing cells, was demonstrated by staining with toluidine blue or coriophosphine O after acid hydrolysis. Argyrophilia was demonstrated both by the Grimelius silver nitrate technic and by the Azzopardi modification of the Bodian method. With these technics, parafollicular cells in the dog and medullary carcinoma of human and rat origin showed nearly identical staining reactions. Fixation in glutaraldehyde was superior to formaldehyde in demonstrating masked metachromasia of normal dog parafollicular cells, while formaldehyde fixation was superior for the demonstration of argyrophilia of granules. In general, the Grimelius method was superior to the Azzopardi modification of the Bodian technic for the demonstration of argyophilia. The results of this study provided further support for the parafollicular cell origin of human and rat medullary thyroid carcinomas and also provided a useful set of histochemical criteria for the diagnosis of this neoplasm.

Animals↗