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Biomedical subjects

J Zhou

Publications and source records attributed to J Zhou.

At least 667 records · Page 37Linked to original sources
PubMed1997

[Comprehensive treatment of bone metastases of breast cancer: a clinical analysis of 70 cases].

OBJECTIVE: Bone metastasis is common in breast cancer patients and its main symptom is bone pain. A series of methods were tried to relieve bone pain and to improve quality of life. METHODS: 70 cases of bone metastases of breast cancer were divided into 2 groups and treated with either chemotherapy, radiotherapy, endocrine therapy or isotope therapy alone or in combination. RESULTS: Endocrine therapy or isotope therapy given alone was more responsive than chemotherapy or radiotherapy alone. When the treatment modalities were used comprehensively, the response rate was 90% as compared to 56.4% in patients given single modality treatment (P < 0.01). CONCLUSION: Endocrine or isotope therapy given alone is more effective than chemotherapy or radiotherapy alone. Patients positive for estrogen and progesterone receptors, are more suitable for endocrine therapy. Comprehensive treatment is recommended.

Adenocarcinoma↗
PubMed1997

[Outward character and inner quality of Bombyx Batryticatus, Carapax Trionycis, Squama Manitis and endothelium corneum Gigeriae Galli after frying].

According to the requirement for outward character in processing traditional Chinese medicines, Bombyx Batryticatus, Carapax Trionycis, Squama Manitis and Endothelium Corneum Gigeriae Galli, which are four kinds of often used traditional Chinese animal medicines, were fried, the contents of amino acids in each kind were determined, and investigate the inner quality of each kind of the processed product was investigated with reference to other standards.

Amino Acids↗
PubMed1997

[The effect of hypertonic saline solution on the rheology after burn-blast combined injury].

To study the effect of hypertonic saline solution on the rheology and putative mechanism, we investigated the changes of plasma viscosity, blood viscosity and reduced viscosity in dogs treated with hypertonic saline solution after burn-blast combined injury and observed the effect of hypertonic saline solution therapy on the viscoelastic property of erythrocyte membranes measured by micropippette aspiration technique in rats with burn. The results showed that the blood viscosity and reduced viscosity increased significantly in dogs after injury, and plasma viscosity also increased significantly at 24 h after injury. Elastic moduli and viscous coefficients of erythrocyte membranes increased obviously in rats after burn. The hypertonic saline solution therapy could significantly improve blood viscosity and reduced viscosity, but it did not significantly improve elastic moduli and viscous coefficients of erythrocyte membranes. These suggested that the hardness of erythrocyte membranes increased, the deform property of erythrocyte membranes decreased, and the blood rheology became worse after burn blast combined injury. Hypertonic saline solution therapy could significantly improve the blood rheology. The effect did not bear a significant relationship with the change of single biomechanics property of erythrocyte membranes. It might be related with other factors.

Animals↗
PubMed1997

[ECG changes during [correction of dring] high G centrifuge training].

ECG of 47 healthy subjects, during G-training on the human centrifuge were recorded and analyzed. Training consisted of 4 G profiles and carried out in 6 d. Subjects were divided into 3 groups according to different anti-G equipment used. The results showed that: high incidence of arrhythmia (87%) was observed, and it increased with the increase of G level; subjects with R/S < 1 under +Gz environment, or HR sharply dropped immediately after the +Gz load have lower G-tolerance; incidence of arrhythmia is lower when advanced anti-G equipment was used.

Acceleration↗
PubMed1997

[Detection of hepatitis G virus RNA in sera in non A-E hepatitis patients using polymerase chain reaction with multiple set primers].

A double polymerase chain reaction, involoving nested primers deduced from NS3-5 regions of the hepatitis G virus (GBV-c/HGV) genome, was developed for a sensitive and specific detection of GBV-C (HGV) RNA. With this method, 10 anti-HGV positive patients with non A-E hepatitis and 10 healthy subjects were tested. Among 10 patients, GBV-C RNA was detected in 9, 8, 4, 9 patients by the NS-3 (1) primers, the NS3(2) primers, the NS4 primers, the NS5(1) primers and the NS5(2) primers respectively. Control sera from 10 healthy subjects did not reveal GBV-C (HGV) RNA by any set of the primers. These data suggest that the detection rate of GBV-C (HGV) RNA is different in various set of primers, the NS3(1) and the NS5(2) primers are superior to other primers and are more suitable for RT-PCR of GBV-C (HGV) RNA.

DNA Primers↗
PubMed1997

[Study on the relationship between the expression of adhesion molecules and the invasiveness of acute myeloid leukemia cells].

OBJECTIVE: To study the relationship between the expression of adhesion molecules CD49d (VLA-4) and CD11a (LFA-1) and the invasiveness of acute myeloid leukemia (AML) cells. METHODS: Peripheral blood and/or bone marrow samples from 50 AML patients were investigated by APAAP and Western blotting method. RESULTS: Extramedullary invasion developed in 32 of 50 patients (64%). The expression of CD49d and CD11a in the invasive group was much higher than that in the non-invasive group (P<0.005), while the difference between the leukemic cells from bone marrow and peripheral blood for CD49d/CD11a expression was not significant. CONCLUSION: AML cells might adhere to and get through vascular endothelium by CD49d/VCAM-1 and CD11a/ICAM-1 adhesion mechanism, and the expressions of CD49d and CD11a were not critically responsible for the release of leukemic cells from bone marrow.

Adolescent↗
PubMed1997

[Relationship between bcl-X(L) expression and sensitivity to chemotherapy in acute myeloid leukemic cells].

OBJECTIVE: To investigate the characteristics and regulation of apoptosis in acute myeloid leukemic cells. METHODS: Flow cytometry, DNA electrophoresis and Northern blot were used to analyze the expression of bcl-X(L) before and after Vp16 treatment, and the relationships between bcl-X(L) expression and apoptotic sensitivity, clinical features and prognosis were also analyzed. RESULTS: Most of the leukemic cells from AML patients overexpressed bcl-X(L) mRNA. The expression of bcl-X(L) was still maintained high level after Vp16 treatment in patients with pretreatment overexpression. These patients were resistant to apoptosis, poor responsed to chemotherapy and associated with adverse prognostic features, such as high WBC counts, local leukemic infiltrations. Bone marrow cells from long-term remission AML patients and normal controls also showed bcl-X(L) downregulation after in vitro Vp16 treatment, but the apoptotic rates were lower than that in nonremissive AML. CONCLUSION: Overexpression of bcl-XL played an important role in leukemogenesis and drug resistance. Downregulation of bcl-X(L) was necessary but not sufficient to initiate apoptosis.

Adolescent↗
PubMed1997

[Analysis of PTH amino acids by micellar electrokinetic capillary chromatography].

A useful method for separation of 19 PTH amino acids by micellar electrokinetic capillary chromatography has been developed. Experiments were performed at 35 degrees C with 14kV power, 50microm x 57cm fused-silica capillary column and UV absorption detector. Each new capillary was washed with 0.1mol/L NaOH and deionized water, each for 15min, before use. Samples were injected into the capillary by applying pressure (3402Pa) for 5s. Absorption detection was at 200nm. The running buffer was made up of 50mmol/L sodium dodecyl sulfate (SDS), 50mmol/L sodium of phosphate and borate (pH 7. 0). The infulence of operation voltage, pH of buffer, temperature and SDS concentration on the amino acids migration has been studied. The coefficients of variaton of migration time and peak area of 19 PTH amino acids were below 1.0% and 5.0% respectively. The detection limits were 0.1-0.25pmol.

Amino Acids↗
PubMed1997

[Determination of changes in membrane phospholipids of rat lung and liver tissues from acute heat stress by high performance liquid chromatography].

The changes in the contents of phosphatidylinositol (PI), phosphatidylserine (PS), phosphatidylethanolamine (PE) and phosphatidylcholine (PC) in lung and liver membrane tissues of rats suffered from acute heat stress were investigated with HPLC. The separation was performed on a mu-Porasil Si column, 300 mm x 3.9 mm i.d., by isocratic elution of acetonitrile : methanol : 85% phosphoric acid (100 : 3 : 1). The phospholipids were detected at 205 nm. The results showed that the contents (mean +/- SD) of PI, PS, PE and PC in normal rats liver were 270.0 +/- 18.2 microg/g, 133.7 +/- 8.6 microg/g, 1465 +/- 132 microg/g and 53.7 +/- 2.8 mg/g respectively, whereas those in heat-stressed rats whose rectal temperature was increased to 42 degrees C and maintained for 15 minutes were decreased by 14%-25% (P<0.01) except PS. When the heat-stressed rats recovered to room temperature for 4h, the contents of phospholipids returned to normal level. The changes of PS and PC in rat lung tissues were identical with those in liver tissues.

Animals↗
PubMed1997

[Studies of magnetic circular dichroism and absorption spectra in alpha-NisO4 x 6H2O crystal].

Alpha-NiSO4 x 6H2O crystals were studied by magnetic circular dichroism (MCD) and absorption spectra measurements in the range lambda = 185-3300nm. The results show that there are three aborption bands, of which the peaks are related to the d-d transitions from the ground state to the excited states. The weak peaks of a progression of the three members were observed between the principal structures lambda = 654nm and lambda = 713nm due to spin-orbit coupling interacion. The weak peaks of a progression of four members were also observed around the new absorption peak at lambda = 585nm in MCD spectra.

English Abstract↗
PubMed1997

Expression of the spinach betaine aldehyde dehydrogenase (BADH) gene in transgenic tobacco plants.

Plasmid pLS9 contains a 1.5-kb of spinach cDNA including its complete open reading frame. The 1.5-kb BADH cDNA was cut from pLS9 using restriction enzyme and was inserted into the expression cassette of plasmid pYH between the CaMV 35S promoter and polyA signal sequence. The 35S-BADH cDNA-polyA fragment of pYH was cloned into a polylinker cloning site of the binary vector pBin19. The resulting plasmid pBinBADH-S was transferred to Agrobacterium tumefacies LBA4404. The tobacco plants were transformed with strain LBA4404 containing pBinBADH-S, and more than ninety kanamycin-resistant transformants were selected. Polymerase chain reaction (PCR) detection showed that more than 60% of the transformed tobacco plants contained the foreign BADH gene. The Western blot analysis, BADH enzymatic assay, specific stain for BADH activity, and the test for salt tolerance showed that BADH gene was normally expressed in the transgenic tobacco plants. The BADH enzymes also presented in chloroplasts and cytosol of the transgenic plants. The transgenic tobacco plants having strong expression of BADH gene had strong ability to tolerate high salt stress.

Aldehyde Dehydrogenase↗
PubMed1997

Production of rhEPO with a serum-free medium in the packed bed bioreactor.

Recombinant CHO (C2) cells producing human erythropoietin (rhEPO) were cultured with DMEM:F12 media containing 5% FBS for 8-10 days in a packed bed bioreactor, then rhEPO was produced with a serum-free medium (SFM-p) which was prepared in our laboratory. The SFM-p medium can support the growth of C2 cells and the production of rhEPO, and furthermore, it easily separates rhEPO from the culture supernatant. The cell culture in a packed bed bioreactor system using SFM-p was maintained in a stable condition for 20-25 days. The expression level of rhEPO was 12-28.4 mg/L. The bioreactor productivity was 71.0 mg/L.d and increased by 12-14 fold over that of the roller bottle. The glucose consumption rate was 21 g/L.d. At the end of 30 days of perfusion circulation, a final cell density of over 3.0 x 10(7)/ml of culture volume was achieved. Since the cells were entrapped in the polyester disk, the culture supernatant contained only a few detachment cells. Variations in lactate and ammonia production in the reactor were observed, and results showed that the productions of lactate and ammonia by the bioreactor were 3.5 g/L and 5 mmol/L, respectively, and did not affect the expression of interest protein. This experiment demonstrates that SFM-p is suitable for the growth and rhEPO production of recombinant C2 in the packed bed bioreactor.

Animals↗
PubMed1997

Study on stress distribution of the condyle.

OBJECTIVE: To investigate the internal state of stress distribution in healthy human condyles and to offer some references for application of the temporomandibular joint (TMJ) biomechanics. METHODS: Six male volunteers were selected to perform CT examination of the TMJ with 1.5 mm-thick sections at 1.5 mm intervals. Three dimensional image reconstruction of the joint was performed in the SUN workstation and the CT data were converted into the decimal system. The three dimensional finite element model mesh generation resulted from 1188 elements and 264 nodes in each condyle. Stress calculation was performed with SAP-V program, and the results were converted into mechanical density scalar. During the study, the internal state of stress distribution on each side condyle in every subject was analysed on the basis of loading on the anterior articular surface of the condyle. RESULTS: Stress was concentrated in the anterior articular surface of the condyle and was highest at the loading point. The condylar cortical bone was the main load-bearing area. The level of stress distribution in the cancellous bone of the condyle was very low. Near condylar neck stress was well-distributed on the cortical. The internal state of stress was essentially symmetrical on the same cuts of both side condyles. CONCLUSIONS: The results showed that three dimensional finite element analysis may indicate the internal state of stress distribution in the condyle, suggesting that a combination of the three dimensional finite element methods and the three dimensional imaging technique may be a useful means for diagnosis and treatment of TMJ diseases.

Adult↗
PubMed1997

Expression and cellular location of endothelin-1 mRNA in rat liver following endotoxemia.

OBJECTIVE: To study the effect of endotoxin on the alteration of the transcription, expression and cellular location of endothelin-1 (ET-1) mRNA in the hepatic tissue. METHODS: Wistar rats were divided into control and endotoxic group. The rats in the control group were injected with saline, and those in the endotoxic group with endotoxin at a dose of 10 mg.kg-1 body wt. ET-1 hepatic homogenate was assayed by radioimmunoassay at 3, 6, 9, 12 and 24 h after endotoxin administration. Dot blot was used to identify and quantify ET-1 mRNA of the hepatic tissue. Hybridization of ET-1 of the hepatic tissue was proceeded at 3, 6, 12 and 24 h after endotoxin administration. RESULTS: ET-1 concentrations and the level of ET-1 mRNA increased rapidly and reached the peak at 6 h, and remained high at 24 h after endotoxin administration. By in situ hybridization, ET-1 mRNA was found in hepatic sinusoids, endothelial cells of portal vein and kupffer cells. CONCLUSIONS: Endotoxin may be the principal stimulating factor for expression of ET-1 mRNA in hepatic tissue. Endotoxin may affect transcription and translation level of ET-1, leading to increase of the synthesis and release of ET-1. The hepatic vascular endothelial cells, hepatic sinusoids and Kupffer cells all synthesize and release ET-1.

Animals↗
PubMed1997

[Expression of nm23/NDPK, integrin, type IV collagenase and uPA in transplanted tumors with various metastatic potential in nude mice and surgical specimens].

OBJECTIVES: To observe the relationship between the metastasis of human lung carcinoma and the expressions of nm23/NDPK, integrins, type IV collagenase and uPA. METHODS: By ABC immunohistochemical staining, we observed the expressions of nm23/NDPK, integrins, type IV collagenase and uPA in four strains of subcutaneous transplanted tumors and 87 surgical specimens for human lung carcinoma. The metastatic potential of four xenografts varied from low to high among PLA-801C, Anip973, PLA-801D, PLA-801DL. The effects of beta 1 antibody and the RGD peptide on the PLA-801DL xenograft were studied in the nude mice. RESULTS: High expression of nm23/NDPK was observed in the four xenografts and specimens from the 87 patients. No association was found between expression of nm23/NDPK and lymph node metastasis. Low expressions of beta 1 subfamily integrins were observed in the four xenografts. beta 1 subunit antibody and RGD peptide had no effect on metastasis of PLA-801DL xenograft. The expressions of type IV collagenase were positively correlated with their metastatic capacity in the four xenografts and surgical specimens from the 87 patients. Anip973 and PLA-801D showed positive staining to uPA, while PLA-801DL and PLA-801C were negative. CONCLUSIONS: The expression of type IV collagenase is positively correlated with the metastatic capacity of lung carcinoma. Although high expression of nm23/NDPK, low expression of integrins, and various expression of uPA were observed, these were not correlated with metastatic capacity of lung carcinoma.

Animals↗