Biomedical subjects

J Wysocki

Publications and source records attributed to J Wysocki.

At least 37 records · Page 2Linked to original sources

PubMed2001

Hepatitis G virus co-infection may affect the elimination of hepatitis C virus RNA from the peripheral blood of hemodialysis patients.

Hemodialysis patients are at risk for hepatitis C virus (HCV) and hepatitis G virus (HGV) infection. The aim of this study was to investigate the possible influence of HGV co-infection on HCV RNA elimination from the peripheral blood of hemodialysis patients. The study involved 144 persons, all with HCV antibodies and HCV RNA. Among 144 patients 24 (16.7%) were positive for HGV RNA. After 2.5 years of observation 80 patients (55.6%) were still HCV RNA-positive. In the latter group 18 patients were co-infected with HGV and 62 were HGV RNA-negative. During 2.5 years of the follow-up study 64 patients eliminated HCV RNA from the serum. In this group only 6 patients were HGV co-infected. None of the HGV-positive patients eliminated HGV RNA from the serum. The higher incidence of HGV co-infection in the group of patients who remained HCV RNA-positive (18/80, 22.5%), in comparison to the group of HCV antibodies-positive patients who lost HCV in the blood (6/64, 9.4%, P < 0.0001) suggests, that the co-infection with HGV may delay the spontaneous elimination of HCV RNA from the blood.

Adult↗

PubMed2000

Detection of DNA, mRNA and early antigen of the human cytomegalovirus using the immunomax technique in autopsy material of children with intrauterine infection.

The present study focuses on the immunomax technique in association with the avidin-biotin-peroxidase complex (ABC) technique and a non-isotopic variation of in situ hybridisation (ISH) for optimal microscopical detection of human cytomegalovirus (HCMV). The studies were performed on an archival paraffin material originating from five children deceased due to intrauterine infection. The results of immunocytochemical and hybridocytochemical studies, with or without amplification using biotinylated tyramine, were compared with the routine histopathological results and results obtained using the polymerase chain reaction (PCR). Early antigen (EA)-HCMV was demonstrated in approximately twice as many cells as detected in the routine staining and also in cells that seemed morphologically intact. The hybridocytochemical studies confirmed the presence of HCMV DNA in cells that were positive in the immunocytochemical tests and, in addition (using the ISH-immunomax technique), in cell nuclei of intact myocardial myocytes. In general, fewer cells manifested the presence of HMCV mRNA than the presence of HCMV DNA. The immunomax technique was found to be more sensitive than the techniques of classical immunocytochemistry or of ISH. The former technique permitted the documentation of a higher number of HCMV replication sites than could be detected using the latter techniques. However, the clinical course of HCMV infection or the cause of death of the children was not directly related to the intensity of HCMV expression in tissues.

Antigens, Viral↗

PubMed2000

[Active infection with Epstein-Barr virus in two children with non-Hodgkin's lymphoma].

Epstein-Barr virus is common in environment. It has an organothropy to lymphoid tissue and liver tissue and the acute infection causes a mononucleosis. The aim of this study was to present two cases in children. In postmortal examination a non-Hodgkin lymphoma was diagnosed. In the first case the course was acute and in the second was chronic. In both cases the unique ethiologic factor was Epstein-Barr virus.

Child↗

PubMed1999

Dimensions of the human vestibular and tympanic scalae.

The cochlear scalae provide a practical access route for the insertion of cochlear implant electrodes. A microanatomical study was carried out on 25 human temporal bones obtained from cadavers. These bones were dissected with the aid of an operation microscope, in which their perilymphatic spaces were filled with coloured latex and further prepared in a formalin stain. Each of the rubber moulds was removed from the osseous matrix using standard otosurgical equipment, and subsequently cut into 1 mm segments. The height and width of the vestibular and tympanic scalae were measured. The results, presented in diagrams, indicate that the vestibular scala is less prominent than the tympanic scala in the basic and middle coil of the cochlea and in the upper coil, they display greater dimensions which could serve as a place for electrode insertion in cochlear implant procedures. In addition, the vestibular and tympanic scalae present alternate dominance in their width and height as corroborated by the calculated coefficients. The results obtained in this study supplement our knowledge of the anatomy of the cochlea thus far lacking a full investigation of the scalae, and could serve as a basis for other studies dealing with the physiology of the organs of hearing.

Adolescent↗

PubMed1999

[Variability of magnitude of the human jugular foramen in relation to conditions of the venous outflow after ligation of the internal jugular vein].

The jugular foramina of the human skull, which are the main route of the venous outflow from the skull, are characterized by laterality. It is based upon predominance of one of the sides. This event presents considerable variability in human population. These differences are probably responsible for various results of ligation of the internal jugular vein, which is performed in some clinical situations in various patients. In order to appreciate the degree of this predominance much more precisely than it is presented in the available literature, a morphological study was carried out on 78 adult human cadavers. With an aid of digital image analysing system we measured a cross-section areas of the jugular foramina of non-macerated skull. We stated that parameters of the jugular foramina didn't differ significantly in respect to age, sex and body side. Predominance of one of the jugular foramina appeared in 83% of cases, and in remaining 17% was absent (it wasn't greater than an error of measurement). If it is present, the predominance of the left and the right side are equally probable. Laterality coefficient was also calculated. It was a ratio between cross-section area of the two jugular foramina: the greater one and the smaller one. Its amount was 1.62 in men and 1.59 in women, however this difference wasn't statistically significant. The range of the laterality was rather considerable because the coefficient differed from 1.02 to 3.6. When the degree of the laterality is high, ligation of the jugular vein on the greater side could by risky. We stated that in our material here were about 2.5% of such cases.

Adult↗

PubMed1999

Studies on tissue expression of HBV in children with chronic hepatitis type B using Immunomax technique.

The study aimed at employing the Immunomax technique to detect the markers of HBV replication (HBcAg and HBV-DNA) in liver biopsy material, obtained from children with chronic hepatitis type B. In line with the currently modified classification of chronic hepatitis and with the increasing potential of antiviral therapy it seemed purposeful to supplement routine staining techniques with studies at the molecular level. Our studies demonstrated the effective detection of both the core antigen and HBV-DNA in liver tissue in children using immunocytochemical techniques and in situ hybridization, amplified with the Immunomax technique. HBcAg was detected in 26 out of 27 liver biopsies from patients with chronic hepatitis type B and with replication of the virus. HBV-DNA was detected in all study children with HBV infection and in 2 out of 5 cases of chronic hepatitis of a distinct etiology. No significant relationships could be found between the detection of tissue HBV markers on the one hand and the intensity of inflammatory lesions or severity of fibrosis on the other.

Biopsy↗

PubMed1998

Distances between the cochlea and adjacent structures related to cochlear implant surgery.

A sound knowledge of the topographic anatomy of the cochlea, especially of the distances between it and the adjacent structures, is essential for otosurgery in general, and in surgical procedures for cochlear implantation in particular. One hundred temporal bones of humans aged from one month to 71 years, of either sex, taken from both body sides (but not bilaterally) were studied by dissection under the operating microscope and exposing the cochlea and its neighbourhood. The minimal distances were measured between the cochlear turns and the internal carotid artery, the bulb of the jugular vein, the facial nerve and the floor of the internal acoustic meatus. It was found that in children aged up to 4 years most of the measured distances were significantly smaller than in older individuals. Only the minimal distance between the basal turn of the cochlea and the floor of the internal acoustic meatus was shorter in small children without statistical significance.

Adolescent↗

PubMed1998

A substance P receptor antagonist (FK 888) modifies gut alterations induced by ionizing radiation.

PURPOSE: We previously reported disturbances of ileal substance P (SP) levels and of characteristics of specific receptors after ionizing radiation associated with disorders of intestinal motility. The aim of this study was to investigate the effect of a SP receptor blockade by FK 888 on gut SP levels and contractile properties after rat irradiation. MATERIALS AND METHODS: Rats were exposed to 6 Gy whole-body gamma-irradiation and injected 1 h post-irradiation with FK 888 for 3 days (0.1 mg/kg/day). Plasma and ileal SP concentrations, ileal muscle SP receptor binding and SP-induced contractions in isolated ileum were investigated 3 and 14 days post-irradiation and FK 888 treatment. RESULTS: Irradiation induced an increase of total SP binding site number at day 3 (1.3-fold) and day 14 (1.6-fold). FK 888 had no effect on SP receptor characteristics in irradiated animals. In contrast, FK 888 treatment caused a reduction of endogenous ileal SP level in mucosal (-29%) and muscularis (-40%) layers at day 3 and these decreases were greater at day 14, -88% in mucosal and -61% in muscularis layers. FK 888 treatment decreased efficacy of ileal contraction in both the control and irradiated rat but surprisingly it increased potency at day 3 and decreased it at day 14 in the irradiated rat. CONCLUSIONS: The findings demonstrate that a SP receptor antagonist could be effective on intestine contractility alteration induced several days after ionizing radiation exposure but not at 3 days after irradiation.

Animals↗

PubMed1998

[Cochleostomy during the intracochlear implantation. Anatomical conditions in children and adults].

Cochleostomy is an opening of the perilymphatic spaces of the cochlea. It is the most important moment in intracochlear implant surgery. There are contradictory opinions in the available literature regarding this surgical intervention. Cochleostomy is performed through the round window, the oval window, the promontory or through the apical turn of the cochlea. A microanatomical study was performed to evaluate which kind of cochleostomy would be most effective and safe for the main structures of the temporal bone. The distances between round window and round window niche structures, the jugular bulb, internal acoustic meatus, posterior semicircular canal, internal carotid artery and cochlear canalicule were measured. It was stated that all the dimensions were smaller in children of up to four years of age and the differences were statistically significant. Cochleostomy through the promontory, especially using the "soft" technique of endosteum opening is safe for all the structures, and in children it also protects the cochlear canalicule.

Adolescent↗

PubMed1998

[Comparative analysis of surgical approaches used during intracochlear implantation with reference to their safety for the important structures of the temporal bone].

A comparative analysis was made of three operative approaches used in cochlear implantations, regarding their safety for main structures of the temporal bone, simplicity and versatility. Anatomical and clinical data from literature were worked out. It was stated that the posterior tympanotomy approach is best because of its allows for good view on the round window niche and its risk for the facial nerve damage is not greater than that of the other routes. The posterior tympanotomy approach is safe for small children because the space between the facial nerve and tympanic ring is not smaller in them than in adults. This route to the tympanic cavity creates also possibilities for widening of the operating area if it is necessary, for example in obstructed and obliterated cochleas. The anterior tympanotomy approach is dangerous for children because of a great risk for facial nerve and jugular bulb. The mastoidotomy-tympanotomy approach is on the other hand very complex, requires special equipment and is not more protective for the facial nerve than posterior tympanotomy approach.

Adult↗

PubMed1998

Effects of fenofibrate on plasma cytokine concentrations in patients with atherosclerosis and hyperlipoproteinemia IIb.

BACKGROUND AND OBJECTIVE: In recent studies atherosclerosis has often been referred to as immune disease. The atherosclerotic plaque consists of large amounts of inflammatory cells, mainly monocytes/macrophages and T lymphocytes. Macrophages activated by low-density lipoproteins (LDL) secrete tumor necrosis factor alpha (TNF-alpha) and interleukin-1 (IL-1) in vitro, while LDL-stimulated T lymphocytes release interferon gamma (IFN-gamma). The aim of this study was to estimate the plasma levels of TNF-alpha, IL-1, IFN-gamma in patients with hyperlipoproteinemia IIb (HLPIIb) and atherosclerosis. Since the fibrates are drugs of choice in HLPIIb, we additionally evaluated the effect of fibrates on the cytokine levels. METHODS: Ten patients with HLPIIb were treated with micronized fenofibrate for 1 month. Before and after treatment, the cytokine levels were measured by the ELISA method. To accurately evaluate cytokine levels, we excluded atherosclerotic patients and control subjects with any inflammatory disease. RESULTS: The initial lipid parameters were as follows: total cholesterol (TC): 6.9 +/- 0.24 mmol/l, triglycerides (TG): 3.44 +/- 0.53 mmol/l, LDL: 4.35 +/- 0.12 mmol/l, and apolipoprotein B (ApoB): 1.62 +/- 0.05 g/l. After 1 month of fenofibrate treatment the parameters decreased to the following values: TC 5.36 +/- 0.42 mmol/l (p < 0.05), TG 1.94 +/- 0.30 mmol/l (p < 0.05), ApoB 1.43 +/- 0.04 g/l (p < 0.01), and LDL 3.75 +/- 0.34 mmol/l (p > 0.05). Before therapy, TNF-alpha levels in atherosclerotic patients were higher than in control subjects, 19.2 +/- 1.6 and 9.9 +/- 1.1 pg/ml, respectively (p < 0.01). After 1-month therapy, TNF-alpha levels in atherosclerotic patients were 9.2 +/- 1.0 pg/ml (p < 0.01). Similarly, the initial levels of IFN-gamma were higher in atherosclerotic patients compared with healthy subjects, 44.4 +/- 5.3, and 19.4 +/- 2.1 pg/ml, respectively (p < 0.01). After fenofibrate therapy, IFN-gamma levels decreased to 24.8 +/- 2.9 pg/ml (p < 0.01). The decreased levels of TC, TG, and LDL correlated with the decreased levels of TNF-alpha and IFN-gamma. CONCLUSION: The results of this study indicate that plasma TNF-alpha and IFN-gamma levels in hyperlipidemic patients are higher than in healthy subjects, and that fenofibrate is effective in decreasing lipids and cytokines in plasma.

Adult↗

PubMed1998

[Needle testicular biopsy in the diagnosis of male infertility].

OBJECTIVES: Evaluation of testicular tissue is very important diagnostic procedures in cryptozoospermia and azoospermia. It is verified the patient for treatment (stimulation spermatogenesis), for micromanipulation ICSI or for insemination donor semen procedure. Surgical biopsy is very popular between andrologist and urologist, but needle biopsy seems to be easier and safer. DESIGN: The authors present their experience with needle testicular biopsy during the diagnosis patients with azoospermia or cryptozoospermia and the men with paraplegia after trauma and without ejaculation. MATERIAL AND METHODS: The study group included 63 biopsies in 58 men with azoospermia and severe oligoasthenozoospermia (cryptozoospermia) and 2 with paraplegia. The specimens were taken from 125 testes under general anesthesia (i.v-Diprivan, Propofol, Zeneca and Fentanyl) using biopsy needle from Hepafix B. Braun Melsungen, Germany. All procedures were performed as a day case. RESULTS: In 95% specimens were adequate for histopathological investigations and for planning the treatment. Only one complication (0, 8%) -small haematoma testis was observed. CONCLUSIONS: The needle biopsy of testicular tissue is sufficiency in histopathological examination, safe for patients and easy for urologists. The total cost is much more lower than cost of surgical biopsy.

Adult↗

PubMed1998

[Percutaneous sperm retrieval for ICSI procedures in men with obstructive azoospermia: ICSI-PESA and ICSI-TESE micromanipulation: our experience].

OBJECTIVES: The sperm retrieval for ICSI procedures is possibly using following procedures: ejaculate sperm, epididymal sperm obtained by microsurgical aspiration or percutaneous puncture and testicular sperm that are obtained by surgical excision or percutaneous biopsy. Percutaneous techniques seem to be rather simple and effective procedures. DESIGN: The authors present their own experiences with percutaneous sperm retrieval for micromanipulation ICSI from the epididymis (ICSI-PESA) and from the testicular tissue (ICSI-TESE) in men with obstructive azoospermia and with reactive impotence. First time in Poland ICSI-PESA was done on April 11, 1996 in Private Infertility Center "Novum", Warsaw. MATERIAL AND METHODS: From April 1996 to the end of January 1998, 10 ICSI-PESA procedures (in 9 couples) and 8 ICSI-TESE (in 6 couples) were performed. In one case ICSI-PESA was performed in man with psychological inability of masturbation during his wife's IVF protocol. All procedures were performed as the day case urology, under general anesthesia. The fine needles No 6 in PESA or biopsy needle from Hepafix Set B. Braun in TESE were used. The therapy of antibiotic and common analgesic drug was used routinely after puncture. RESULTS: The effectiveness of obtaining sperm for micromanipulation were 100% ICSI-PESA and 75% ICSI-TESE. The pregnancy rate in PESA was 50% and 5 healthy children were born. In TESE only 1 woman (17%) was pregnant, but early spontaneous miscarriage was reported. No surgical and anesthesiological complications were noticed. CONCLUSIONS: Obtaining sperm for micromanipulation ICSI using percutaneous epididymal puncture or testicular tissue needle biopsy seems to be effective and safe for patients with obstructive azoospermia or reactive impotence.

Arterial Occlusive Diseases↗

PubMed1998

[Correlations between topography of the main structures of the temporal bone and the location of the sigmoid sinus].

Topographical relations between main structures of the temporal bone are important for otosurgeons. There were reports aiming at establishing some laws in the temporal bone building, that would allow to anticipate the location of the important structures in the temporal bone. It is obvious nowadays that precise and absolute rules do not exist. In the present study the aim was to estimate if there are statistically significant correlations between some dimensional parameters of the temporal bone. It was stated that the smaller the distance between the sigmoid sinus and the external auditory meatus, the higher the jugular bulb is situated. Coefficient in this correlation was 0.46. However, no correlation was found between the location of the sigmoid sinus and the facial nerve on one hand and the internal carotid artery on the other. It is important for otosurgeons to know that the prominent sigmoid sinus does not determine existence of a narrow facial recess.

Adolescent↗

PubMed1998

[Levels of tumor necrosis factor alpha in serum of patients with hyperlipoproteinemia IIB before and after micronized fenofibrate therapy].

UNLABELLED: Tumor necrosis factor alpha (TNF alpha) secreted by activated macrophages stimulates proliferation and migration of vascular smooth muscle in atherogenesis. Up to now, the effect of fibrates on concentration of TNF-alpha has not been investigated. The aim of this study was to estimate TNF-alpha plasma concentration in healthy subjects before and after fenofibrate therapy in patients with hyperlipoproteinaemia IIb and to correlate their levels with plasma concentration of total cholesterol (TC), low density lipoproteins (LDL) and apolipoprotein B (ApoB). METHODS: 10 patients with hyperlipoproteinaemia IIb were treated with micronized fenofibrate (Lipanthyl 200 m-Fournier) for 1 month. Cytokines levels before and after therapy was measured by the ELISA method with Genzyme kits. RESULTS: The levels of lipid parameters at the onset study were as follows: TC: 265.4 +/- 9.4 mg%, TG: 344 +/- 53 mg%, LDL: 167.2 +/- 4.7 mg%, and ApoB: 1.62 +/- 0.05 g/l. After 1-month therapy with Lipanthyl 200 m the parameters decreased: TC 206 +/- 16 mg% (p < 0.05), TG: 194 +/- 30 mg% (p < 0.05), ApoB: 1.43 +/- 0.04 g/l (p < 0.01), and LDL: 144.4 +/- 12.9 mg% (ns). The decreased level of TC, TG and LDL correlated with the decreased concentration of TNF alpha. Before treatment the TNF concentration was 19.2 +/- 1.6 pg/ml and was higher than the concentration of control subjects (9.9 +/- 1.1 pg/ml, p < 0.01). After 1-month therapy the level of TNF alpha in blood from patient was 9.2 +/- 1.0 pg/ml (p < 0.01). The results of this study indicate that the concentrations of TNF alpha in plasma from hyperlipidemic patients are higher than concentrations of healthy subjects. Fenofibrate decreased the levels of this cytokine. This effect may be of significance for the treatment of HLPIIb and of atherosclerosis prevention.

Adult↗

PubMed1998

[Variability of the round window niche entry in children and adults].

The round window niche entry (fossula fenestrae cochleae, fossula fenestrae rotundae) is one of the main structures in the labyrinthine wall of the tympanic cavity. Its variability is fairly significant and very important in otosurgery. In order to learn more about the morphology of the round window niche entry, a microanatomical study on 100 temporal bones obtained from cadavers aged 0-71 y. was performed. A standard set of otosurgical equipment was used. Five morphological forms of the round window niche entry were differentiated: triangular, semicircular, ovally horizontal, ovally vertical and round ones. Triangular forms were observed only in children of over 4 years of age and round forms almost only in young children, of age below this borderline (the beginning of pneumatization of the temporal bone). The differences between these two groups were statistically significant.

Adolescent↗

PubMed1998

Mastoid emissary in Indian skulls.

Two hundred and eleven Indian skulls originating from medieval times, South of Peru, were studied in respect to variability of the mastoid emissary. Occurrence and localisation of the external orifice of the mastoid emissary were studied in three distinct and well-separated populations, living in the three different places: Villa el Salvador, Tablada de Lurin and Paracas. Most of the skulls presented considerable degree of artificial deformation. External orifice of the mastoid emissary was multiple in most of the skulls, especially in Villa el Salvador and Paracas populations. Mastoid emissaries in Indian were situated generally higher than in European, especially regarding the Paracas population. Significant sex differences were present in this material, regarded as a whole (summarized results for the three populations), what is in agreement with previous observations on European skulls. It indicates that even in cases of relatively deep deformation, some characteristic features of sex dimorphism of the human skull are noticeable. Additionally, the three studied populations, which were well separated from each other and lived alone, differed significantly regarding localization of the mastoid emissaries.

Cephalometry↗

PubMed1998

[The multiple promontory bridge].

Anatomical investigations of one hundred human temporal bones revealed a significant variability of the promontory bridge (ponticulus promontorii). In twelve bones double and in two-triple promontory bridge was observed. The triple promontory bridge had not been previously described in available literature. In all the cases the double or triple promontory bridges were observed only on the temporal bones obtained from cadavers of up to four years of age. It confirms previous statements suggesting that development of this structure is connected with the process of pneumatization of the statements suggesting that development of this structure is connected with the process of pneumatization of the temporal bone, which obviously starts in 3-4 year of life.

Adolescent↗