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Biomedical subjects

J Wu

Publications and source records attributed to J Wu.

At least 1,495 records · Page 83Linked to original sources
PubMed1989

Tissue-specific determinants of human atrial natriuretic factor gene expression in cardiac tissue.

Elements controlling tissue-specific expression of the human atrial natriuretic factor gene have been examined in primary cultures of neonatal rat cardiocytes. When a 68-base pair fragment from human atrial natriuretic factor (hANF) 5'-flanking sequence (positions -400 to -333) was placed upstream from the herpes simplex thymidine kinase promoter linked to a bacterial reporter gene (chloramphenicol acetyltransferase), a tissue-specific positive regulatory effect was observed in atrial as well as ventricular cardiocytes but not in nonmyocardial cells. The cis-acting element in this fragment was orientation- and position-dependent. Examination of nuclear protein extracts for the presence of factors capable of interacting with the 5'-flanking sequence of the hANF gene revealed a cardiocyte-specific factor which bound to the 68-base pair fragment. This association was both tissue- and sequence-specific. These findings indicate that a cis-acting element present in the proximal 5'-flanking sequence confers tissue-specific expression upon the hANF gene, possibly through association with a cardiac-specific nuclear protein.

Atrial Natriuretic Factor↗
PubMed1989

Potential difference responses to nutrient K+, Na+ and Cl- changes with varying nutrient HCO3- in resting frog stomach.

The effects of changes in nutrient concentrations of K+, Na+ and Cl- on the transmucosal potential difference (PD) and resistance were compared for 25 and 5 mM nutrient HCO3- in resting fundus. With 25 mM HCO3-, increase of K+ from 4 to 40 mM, decrease of Na+ from 100 to 10 mM and decrease of Cl- from 81 to 8.1 mM gave, 10 min after the change, delta PD values of -23.2, -15.1 and -21.3 mV, respectively. With 5 mM HCO3-, the same changes in nutrient ion concentration gave delta PD values of -11.9, -9.4 and -10.0 mV, respectively. From these results, in going from 25 to 5 mM HCO3-, it follows that the resistances of the ionic pathways for K+, Na+ and Cl- increased. The anomalous PD response following the increase in nutrient K+ from 4 to 40 mM with 5 mM nutrient HCO3- gave further evidence that the resistance of the simple K+ conductance pathway increased prior to the increase to 40 mM K+. The fact that 2 mM Ba2+ in the 25 mM HCO3- nutrient gave a smaller increase in resistance, compared to the decrease in nutrient HCO3- from 25 to 5 mM, supported the inference that resistances of ion pathways other than that of the K+ pathway increased.

Animals↗
PubMed1989

Regional cerebral glucose metabolic rate in human sleep assessed by positron emission tomography.

The cerebral metabolic rate of glucose was measured during nighttime sleep in 36 normal volunteers using positron emission tomography and fluorine-18-labeled 2-deoxyglucose (FDG). In comparison to waking controls, subjects given FDG during non-rapid eye movement (NREM) sleep (primarily stages 2 and 3) showed about a 23% reduction in metabolic rate across the entire brain. This decrease was greater for the frontal than temporal or occipital lobes, and greater for basal ganglia and thalamus than cortex. Subjects in rapid eye movement (REM) sleep tended to have higher cortical metabolic rates than waking subjects. The cingulate gyrus was the only cortical structure to show a significant increase in glucose metabolic rate in REM sleep in comparison to waking. The basal ganglia were relatively more active on the right in REM sleep and symmetrical in NREM sleep.

Brain↗
PubMed1989

The effect of therapeutic ultrasound on electrophysiological parameters of frog skin.

There are two groups of mechanisms through which ultrasound can affect biological systems, those of thermal origin and others of nonthermal origin. Since in almost every therapeutic application of ultrasound, movement of ions across cellular membranes is involved, it becomes important to study the effect of ultrasound on active and passive ionic conductance. In order to differentiate between thermal and nonthermal effects, a study was conducted on model systems in which the effect of temperature is known. The well-known sodium transporting epithelium, the epidermis of abdominal frog skin, was investigated and the effect of therapeutic ultrasound on its electrophysiological properties was determined. It was found that under open circuit conditions, irradiation of the skin with 1 MHz cw (60-480 mW/cm2) ultrasound caused a significant decrease (5-50%, depending on the applied power) in the transepithelial potential and resistance at room temperature (20-22 degrees C). Under short circuit conditions, also at room temperature, there was an increase in total ionic conductance (20-250%, depending on the applied power) and a decrease in the net actively transported current, measured as the short circuit current. These effects are reversible within the range of powers used. Furthermore, it was found that the magnitude of the observed changes was strongly dependent on the perfusion rate and the gas content of the bathing medium. The effect of ultrasound diminished in the presence of CO2 and was enhanced with faster perfusion rates. Pulsed ultrasound delivered at the same energy (Isata) as that of cw caused a significantly larger effect. At lower temperatures (12-14 degrees C) the effect of ultrasound was reduced. Analysis of the data reveals that the effects of ultrasound on ion transport reported here are not primarily of thermal origin but are probably due to cavitation and related effects, such as microsteaming.

Animals↗
PubMed1989

Mammalian pre-mRNA branch site selection by U2 snRNP involves base pairing.

SV40 early pre-mRNA is alternatively spliced to produce large T and small t mRNAs by use of different 5'-splice sites and a shared 3'-splice site. The large T splicing pathway uses multiple lariat branch sites, whereas small t splicing, constrained by its small intron size, can use only one. We exploited this situation to test the hypothesis that RNA-RNA base pairing between U2 snRNA and the branch site sequence is important in mammalian pre-mRNA splicing by constructing and analyzing several mutations in the small t pre-mRNA branch site (UUCUAAU). All of the mutations resulted in substantial decreases in small t splicing relative to large T. To test whether these effects resulted from decreased base pairing with U2 snRNA, compensatory mutations were introduced at the appropriate positions (nucleotides 34-36) in a cloned human U2 gene. All branch site mutations tested (four separate single base substitutions and two triple mutations) were suppressed (i.e., small t splicing was increased) by the appropriate U2 mutations. These results establish that recognition of the poorly conserved mammalian pre-mRNA branch site sequence by U2 snRNP can involve base-pairing.

Animals↗
PubMed1989

Photosynthetic Plasticity in Flaveria brownii: Growth Irradiance and the Expression of C(4) Photosynthesis.

Photosynthesis was examined in leaves of Flaveria brownii A. M. Powell, grown under either 14% or 100% full sunlight. In leaves of high light grown plants, the CO(2) compensation point and the inhibition of photosynthesis by 21% O(2) were significantly lower, while activities of ribulose 1,5-bisphosphate carboxylase/oxygenase and various C(4) cycle enzymes were considerably higher than those in leaves grown in low light. Both the CO(2) compensation point and the degree of O(2) inhibition of apparent photosynthesis were relatively insensitive to the light intensity used during measurements with plants from either growth conditions. Partitioning of atmospheric CO(2) between Rubisco of the C(3) pathway and phosphoenolpyruvate carboxylase of the C(4) cycle was determined by exposing leaves to (14)CO(2) for 3 to 16 seconds, and extrapolating the labeling curves of initial products to zero time. Results indicated that approximately 94% of the CO(2) was fixed by the C(4) cycle in high light grown plants, versus approximately 78% in low light grown plants. Thus, growth of F. brownii in high light increased the expressed level of C(4) photosynthesis. Consistent with the carbon partitioning patterns, photosynthetic enzyme activities (on a chlorophyll basis) in protoplasts from leaves of high light grown plants showed a more C(4)-like pattern of compartmentation. Pyruvate, Pi dikinase and phosphoenolpyruvate carboxylase were more enriched in the mesophyll cells, while NADP-malic enzyme and ribulose 1,5-bisphosphate carboxylase/oxygenase were relatively more abundant in the bundle sheath cells of high light than of low light grown plants. Thus, these results indicate that F. brownii has plasticity in its utilization of different pathways of carbon assimilation, depending on the light conditions during growth.

Journal Article↗
PubMed1989

c-Ha-ras gene bidirectional promoter expressed in vitro: location and regulation.

Increased transcriptional activity of the c-Ha-ras gene product is correlated with induction of several important human tumor types. For this reason, we have investigated the nature of the c-Ha-ras promoter and the factors that regulate its expression. Using S1 and primer extension analysis of c-Ha-ras RNA from EJ cells, we have identified 18 initiation sites within an upstream exon (exon -1) whose 3' end (the donor splice site [D]) is located 1,105 base pairs (bp) upstream of the ATG codon. The furthest-upstream initiation site is located -191 bp relative to D, and the furthest downstream is located -16 bp relative to D. Transient expression assays, in which a series of mutants spanning this region were ligated to a promoterless chloramphenicol acetyltransferase vector, functionally confirmed the position and extent of this promoter. Mutational analysis further located a 47-bp element located between -243 and -196 relative to D that up-regulated transcriptional activity of the promoter region by 20- to 40-fold. This region contained both a GC box known to bind SP1 and a CCAAT box. Insertion of a simian virus 40 enhancer 5' to the promoter up-regulated transcription from each initiation site by approximately 10- to 20-fold. We have also localized, both by chloramphenicol acetyltransferase assay and by S1 analysis, a strong promoter operating in the direction opposite that of the gene and originating immediately 5' to the 47-bp regulatory region. The reverse promoter was found to have nine initiation sites between -248 and -278 relative to D.

Animals↗
PubMed1989

Positron emission tomography of the cerebellum in autism.

On the basis of neurological evidence that autistic patients have fewer Purkinje and granule cells in the cerebellum as well as vermal cerebellar hypoplasia, the authors tested the hypothesis that autistic patients have cerebellar hypofunctioning. They used positron emission tomography of the cerebellum with 18F-labeled 2-deoxyglucose to study seven autistic patients and eight age-matched control subjects. The results showed no significant difference in mean cerebellar glucose metabolism between the two groups, but all mean glucose rates of the autistic patients were either equal to or greater than those of the control subjects. The implications of these findings are discussed.

Adult↗
PubMed1989

Expression of the atrial natriuretic peptide gene in human fetal heart.

Expression of the human atrial natriuretic peptide (hANP) gene was examined in tissues of 19 to 28-week-old human fetuses. As expected the fetal atria express the hANP gene at a high level, accruing substantial quantities of ANP immunoreactivity and ANP mRNA. The neonatal ventricle also expresses the hANP gene at a significant level. ANP mRNA levels in the ventricle were, on the average, about 20% of those in the right atria. ANP immunoreactivity in ventricle was less that 5% of that in the right atria, suggesting important differences in the way these tissues synthesize and store the ANP peptide. Much lower levels of hANP transcripts were also detected in the lung and aortic arch. Analysis of the 5' termini of cardiac hANP transcripts using three independent techniques suggests the presence of two transcription start sites. A major start site is located approximately 28 basepairs downstream from the primary TATA sequence. A second minor start site is positioned about 110 basepairs further upstream. Immunocytochemistry and in situ hybridization analysis indicate that the hANP gene is expressed in a homogeneous distribution throughout the atrial myocardium. Diffuse low level expression is also present within the ventricular myocardium. In addition, there are scattered foci of increased expression in the ventricle which tend to be concentrated in the subendocardium of that organ. These findings indicate that the developing human ventricles as well as the atria possess the capacity to express the hANP gene at a substantial level and suggest that the ventricle may contribute significantly to the circulating pool of plasma ANP.

Atrial Natriuretic Factor↗
PubMed1989

[A pathologic study of adenohypophyseal growth hormone cells in the rabbit after severe burn].

The growth hormone(GH), produced by the growth hormone cell in pars distalis of the adenohypophysis, acts on the sugar, protein and fat metabolism in various degrees. After trauma, the GH has relations with the energy supply, the maintenance of nitrogen balance, the tissue repair and the body resistance. However, pathological study on the GH cell after burn injury is rare in the literature so far. The purpose of the present investigation is to take a dynamic observation on the ultrastructural changes of the rabbit GH cell after napalm burn within one week. 46 male rabbits were used and divided into two groups, napalm burn group (N = 36) and control group (N = 10). The former is inflicted with 3rd degree burn covering 30% TBSA. The animals of former group were sacrificed at 3, 6, 12, 24, 48, 72, 96, 120, 168 hours postburn respectively. Using the light and electron microscopy and stereological method, the results revealed that: (1) the synthesis activity in GH cell was enhanced, the process of secretion was rapid, and the rate of granule maturation was increased; (2) the nude GH granules were found both in the sinusoids and the endothelial cells; (3) the newly formed mitochondria may be originated from the Golgi complex, and the newly formed Golgi complex from the reutilization of the plasma membrane components; (4) some endothelial cells manifested degeneration, and the others showed in active condition; (5) under the light microscopy, the distribution of the lower tint-phil GH cells had its regional-characteristics.

Animals↗
PubMed1989

[Effect of electro-acupuncture (EA) on response of cerebellar cortex to slow pain].

Experiments were performed on cats under chloralose anaesthesia and immobilized by Flaxedil. The right saphenous nerve was stimulated with single rectangular electric pulse of 30V and A-fibers were blocked selectively by the anodal current so as to elicic the C-fiber input only. The cerebellar cortical field potential evoked by C-fiber input (C-CEP) was recorded on the contralateral vermian VI lobule surface. C-CEP can be inhibited obviously by injecting the morphine (0.5 mg/kg) intravenously. It indicated that C-CEP had the close concern with the slow pain information and might be regarded as the index of the slow pain. The effects of the EA at the "Zusanli" point on C-CEP were observed. The results as following: 1. The amplitude of C-CEP decreased obviously after the EA at the ipsilateral "Zusanli" (strength: 10V, duration: 0.2 ms, frequency: 5 Hz). 2. From 5V-30V the more the strength of the EA was, the more the inhibitory efficiency was. 3. The inhibitory efficiency of the EA at the ipsilateral "Zusanli" point on C-CEP was stronger than at the contralateral, and the strongest at the bilateral.

Animals↗
PubMed1989

[Atrial natriuretic polypeptide in the human and rat spinal cord].

The widespread distribution of atrial natriuretic polypeptide (ANP) immunoreactive material was demonstrated in the human and rat spinal cord, using specific immunogold-silver staining technique and radioimmunoassay. Immunogold-silver staining studies showed that ANP immunoreactive neurons were quite prevalent in the human spinal cord. ANP immunoreactive neurons were largely found in the ventral horn and less in the dorsal and lateral horns. ANP immunoreactive material was absent with anti-ANP serum preabsorbed with ANP. In rats, radioimmunoassay demonstrated that ANP immunoreactive material was present in whole spinal cord. The highest concentration was found in the sacral spinal cord (21.9 +/- 4.48 ng/g tissue), the moderate in the lumbar (3.78 +/- 0.74 ng/g tissue) and the lowest in the cervical and thoracic (0.58 +/- 0.14 and 0.46 +/- 0.21 ng/g tissue, respectively). Gel chromatography and reverse-phase HPLC revealed multiple molecular forms in the rat spinal cord, but the main molecular form was the 28 amino acid rANP. In vivo study showed that ANP was released from the rat spinal cord by depolarizing concentration of potassium.

Animals↗
PubMed1989

2-Mercaptoethanol and n-acetylcysteine enhance T cell colony formation in AIDS and ARC.

One contributing factor to the loss of T cells in AIDS may be the impaired ability of T cell precursors to expand, as reflected in a decreased ability of patient cells to form T cell colonies in agar. We and others have noted such a defect in people with AIDS and ARC, and have found that suppressor cells and suppressive plasma contribute to decreased T-CFC formation. We report here that the reducing agents 2-mercaptoethanol (2-ME) and n-acetyl cysteine (NAC) can enhance colony formation in vitro. In part, 2-ME can reverse the defect in T cell colony-forming cells (T-CFC) formation by overcoming the effect of suppressor cells. In a group of 46 AIDS patients, T-CFC formation was initially 42 +/- 8% (mean +/- s.e.) that of control levels. 2-ME caused an increase of 401 +/- 76% in T-CFC formation which was significantly greater than the increase in control T-CFC formation; it also significantly enhanced T-CFC formation by cells from ARC patients. Suppressor cell activity from ten AIDS patients decreased from 58 +/- 21% to 12 +/- 10% when 2-ME was added. Similar data were obtained from 14 ARC patients. NAC, a related antioxidant with low toxicity, also enhanced T-CFC in cells of AIDS and ARC patients. Vitamin C generally did not increase T-CFC formation. The data suggest that certain antioxidants such as 2-ME and NAC may be useful in treatment protocols to enhance T cell numbers in patients with AIDS or ARC.

AIDS-Related Complex↗
PubMed1989

Acquired cytomegalovirus infection of breast milk in infancy.

A cross-sectional study was conducted to examine the rate of cytomegalovirus (CMV) shedding through breast feeding and the rate of acquired infection by the infants. Among the 104 seropositive breast-feeding mothers, CMV was recovered in the milk in 19 cases, with a milk shedding rate of 18.3%. Isolation of CMV from the urine of the infants of the 188 seropositive mothers found 36 positive cases. Of the 36 cases, 15 of 19 fed on CMV-positive milk were infected through breast-feeding, with an infection rate of 78.9%; 11 of the 85 fed on CMV-negative milk were infected, the infection rate being 12.9%; and 10 (11.9%) of 84 fed on cow's milk were infected. These demonstrate that the rate of infection in infants fed on virus-positive milk was significantly higher than those fed on virus-negative milk or cow's milk (P less than 0.005).

Breast Feeding↗
PubMed1989

[Electric responses evoked by C-fiber input from saphenous nerve in cat cerebellar cortex].

When the A-fibers of the saphenous nerve were stimulated at a lower intensity which elicited the A-fiber input only, an evoked potential (A-CEP) which consisted of an early component with latency of 11.8 +/- 3.5ms and a late component with latency of 312.1 +/- 17.5ms could be recorded on the surface of the cerebellar cortex in cat. When A- and C-fibers were activated by stimulation at the strength of C-fiber suprathreshold, the latency and configuration of the evoked potential had no significant difference from one of the A-CEP. After A-fibers were blocked by the polarizing current, stimulation at the strength of C-fiber suprathreshold could evoke a characteristic C-CEP with latency of 134.2 +/- 18.4ms. In general, C-CEP whose maximal amplitude was recorded on the vermain lobule VI was a positive-negative potential and its phases were reversed in the deep layer of the cerebellar cortex. C-CEP had a smaller amplitude, a longer latency and a lower following frequency than A-CEP had. In comparison with A-CEP, C-CEP had a high susceptibility to the morphine. The results showed that C-CEP which was evoked by the selective C-fiber input and generated in the cerebellar cortex seemed to be a response to the information of the slow pain input. It was suggested that C-fiber input could reach the cerebellar cortex and elicited an evoked potential. When A- and C-fiber were activated at the same time, C-CEP might be inhibited by the A-fiber input.

Animals↗
PubMed1989

[Endoscopic bipolar circumactive electrocoagulation in acute upper gastrointestinal bleeding].

Bipolar circum-active probe (BICAP) is a new endoscopically deliverable bipolar electrocoagulator. 57 patients with acute upper gastrointestinal bleeding have been treated by the endoscopic BICAP and the value of the technique in the control of acute bleeding has been assessed. 57 patients underwent 47 qg probe applications with a mean value of 8.3 for each case. Immediate hemostasis was achieved in out of 57 cases (93%). Rebleeding after hemostasis by BICAP was noted in 4 out of 53 cases (7.5%). There were no complications related to the procedure. It is an important technique for the control of acute upper gastrointestinal bleeding.

Adolescent↗