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Biomedical subjects

J Wu

Publications and source records attributed to J Wu.

At least 523 records · Page 29Linked to original sources
PubMed2000

[Cost-effectiveness analysis of Beijing Fangshan cardiovascular prevention program in 1992 - 1997].

OBJECTIVE: To determine whether a community-based cardiovascular disease (CVD) intervention program, undertaken over six years, was cost-effective. METHODS: Based on Beijing Fangshan Cardiovascular Disease Comprehensive Prevention Program, the cost for intervention and expenditure saved from caring for CVD in the communities with intervention from 1992 to 1997 were calculated, and cost-effectiveness analysis was performed using disability-adjusted life years (DALYs) gained as an indicator of effectiveness. RESULTS: The cost for one DALY gained reduced gradually from 1992 to 1997, with an average ratio of cost to effectiveness of four to one (4:1). It cost annually 1,586.00, 1,380.20, -2,350.80, -905.30, -1,495.60 and -1,766.70 RMB yuan for one life-year saved, from 1992 to 1997 respectively, in a gradually decreasing trend with the increase in length of intervention. After intervention for two years, ratio of cost to effectiveness has become negative since 1994, which meant a positive benefit from intervention. Sensitivity analysis showed that ratio of cost to effectiveness was little sensitive to the changes in discount rate, weight of age and increase in cost of hospitalization for stroke and coronary heart disease, which reflected its reliability. CONCLUSION: Community-based comprehensive intervention for CVD in rural population is cost-effective.

Aged↗
PubMed2000

[Effects of phoxim and fenvalerate on TTX-S and TTX-R sodium channels in the DRG neurons of adult rat].

OBJECTIVE: To study the joint neurotoxic effects of phoxim (Pho) and fenvalerate (Fen) on tetrodotoxin-sensitive (TTX-S) and tetrodotoxin-resistant (TTX-R) Na(+) currents in dorsal root ganglion (DRG) neurons of adult rat. METHODS: Whole cell patch clamp technique was used to test the effects of Pho and Fen on TTX-S and TTX-R sodium currents in DRG neurons. RESULTS: The inactivation of TTX-R sodium channel was obviously slowed down by Fen. The tau(Na) of peak currents at doses of 10, 50 and 100 micromol/L Fen and control groups were (8.10 +/- 2.41) ms, (11.78 +/- 2.76) ms, P < 0.01, (8.76 +/-1.94) ms, P < 0.05 and (6.41 +/- 1.32) ms respectively. The inactivation of TTX-R sodium channel tail currents was also significantly delayed by Fen. The tau(Na) of the tail currents at doses of 10, 50, 100 micromol/L Fen and control groups were 6.11 +/- 0.52 (P < 0.05), 7.82 +/- 0.82 (P < 0.05), 7.23 +/- 1.09 (P < 0.05) and (4.91 +/- 0.97) ms separately. As compared with TTX-R sodium channel, the TTX-S sodium channel was less responsive to Fen exposure, which only led to slowly decay TTX-S sodium tail currents. There was no any effect of Pho on the TTX-S and TTX-R sodium channels. The mixed treatment of a Pho and Fen did not show joint effect on the sodium currents. CONCLUSION: Both the peak and tail currents are changed by Fen, however, Fen has more remarkable effects on TTX-R than on TTX-S sodium channel. The combined exposure to Pho and Fen shows no joint effect on the sodium channel.

Animals↗
PubMed2000

[Prolonging the survival of transplanted alloskin by transferring the CTLA(4)Ig locally with recombinant adenovirus vector in mice].

OBJECTIVE: To find a simple and practical way to prolong the survival of transplanted alloskin to cover the burn and other wounds. METHODS: A recombinant CTLA(4)Ig-Adenovirus expression vector was constructed by means of homo recombination, and then transferred either into cultured murine skin grafts ex vivo or local wound surface. The expression of CTLA(4)Ig in cultured skin was immunohistochemically determined. Moreover, the survival time of transplanted alloskin from Kunming mice to Balb/c mice was evaluated. RESULTS: The CTLA(4)Ig-Adenovirus vector could be successfully transferred and expressed in the cultured murine skin, and the locally used recombinant CTLA(4)Ig-Adenovirus vector could significantly prolong the survival of the transplanted alloskin from 8 d to 21 d. CONCLUSION: Local transfection of the CTLA(4)Ig-Adenovirus vector is a simple and effective way to prolong the survival of alloskin grafts.

Abatacept↗
PubMed2000

[The postburn change in splenic T lymphocyte transmembrane signal transduction and its relationship with the secretion of IL-2 and IL-10 in severely scalded mice].

OBJECTIVE: To explore the postburn functional change in splenic T lymphocytes and the secretory rules of IL-2 and IL-10 in severely scalded mice, and to look for the explanation of the change by means of T lymphocyte transmembrane signal transdunction study. METHODS: The changes in the antigen receptors on T cells (TCRalpha/beta), assisting stimulating molecule (CD28) and the activities of GTPase, protein tyrosine kinase (PTK) and protein kinase C (PKC) that participated in the transmembrane signal transduction were detected, so as to observe the proliferation and transformation function of T cells at different post-burn periods and the secretion of IL-2 and IL-10. Furthermore, the changes in every signal transduction molecules were analyzed with the consideration of their relationship with the change in T cell function activities. RESULTS: The postburn positive rates of the expressions of TCRalpha/beta and CD28 on T cell membrane decreased. The postburn GTPase activity and membrane PTK activity were all suppressed but recovered at 168 postburn hours. Membrane PKC activity exhibited a dual-phase change (increase following decrease), which was closely related to IL-10 level. CONCLUSION: The postburn changes in TCRalpha/beta, CD28 on T cell membrane molecules and transmembrane signal transduction enzymes were important factors contributing to the decrease in IL-2 secretion, suppressed T cell function and the dual-directional changes in IL-10 secretion.

Animals↗
PubMed2000

[Antiphospholipid thrombosis syndrome and acquired activated protein C resistance].

OBJECTIVE: To explore the correlation between antiphospholipid antibodies (APA) activated protein C resistance (APCR) and antiphospholipid thrombosis (APL-T) syndrome and investigate the mechanism of thrombosis in APL-T syndrome. METHODS: ELISA, APTT-LA and APTT+/-APC methods were used to detect anticardiolipin antibodies (ACA), lupus anticoagulants (LA) and APCR in 20 APL-T syndrome patients respectively. RESULTS: Twenty patients were diagnosed as APL-T. The patients were classified in three ways. According to etiology, 14 of 100 systemic lupus erythematosus (SLE) patients were diagnosed as "secondary" APL-T, and 6 of 16 "unexplained" thrombosis and spontaneous abortion as "Primary" APL-T syndrome. According to antibodies, 12 of 20 patients with positive LA were diagnosed as lupus anticoagulant thrombosis syndrome (LA-T), 12 of 20 patients with positive ACA as anticardiolipin antibodies thrombosis syndrome (ACA-T). According to clinical presentation: 7 of 20 patients with deep vein thrombosis were classified as type I APL-T, 3 of 20 patients with cerebrovascular thrombosis as type II, 1 of 20 patients with vein cerebrovascular thrombosis and spontaneous abortion as type IV (mixing), and 9 of 20 patients with spontaneous abortion as type V (fetal wastage type). The ACAs and LA are strongly associated with APL-T. There is close relationship between APCR and APL-T. CONCLUSION: The acquired APCR might be an important reason for thrombosis in APL-T caused by APA and LA.

Activated Protein C Resistance↗
PubMed2000

[Exploration of gene expression profiles of CD(34)(+) hematopoietic stem/progenitor cells based on large scale sequencing].

OBJECTIVE: To set up a large scale sequencing system and explore the gene expression profiles of CD(34)(+) hematopoietic stem/progenitor cells (HSPCs). METHODS: CD(34)(+) cells were isolated from umbilical cord blood and subjected to cDNA library construction. A primary profile of gene expression in HSPCs was emerged by EST sequencing and bioinformatics analyzing. RESULTS: Among 9,866 ESTs thus obtained, 7 476 meaningful ESTs were clustered into 2,060 unique sequence species (USSs), representing 1,054 known gene species and 1,006 unknown gene fragments. The 1054 known genes were divided into 8 categories: (1) hematopoiesis associated: 73, (2) chromatin structure and cell division/apoptosis: 91, (3) signal transduction and receptors: 111, (4) cell structure/mobility: 48, (5) cell/organism defense/homeostasis: 41; (6) Gene expression (transcription, translation and modification): 265, (7) metabolism: 192; and (8) unclassified: 233. CONCLUSION: A gene expression profile including 1,054 known genes and 1006 new gene fragments of HSPCs was primarily obtained, which may lay a basis for the further study on the molecular mechanism of hematopoiesis regulation and provide candidates for new gene cloning.

Antigens, CD34↗
PubMed2000

[Detection of soluble endothelial protein C receptor (sEPCR) in patients with CHD, DM and SLE].

OBJECTIVE: To evaluate the clinical significance of plasma soluble endothelial protein C receptor (sEPCR) level in patients with coronary heart disease (CHD), diabetes mellitus (DM) and systemic lupus erythematosus (SLE). METHODS: ELISA of antibody-sandwiched principle was used to detect sEPCR in 34 patients with CHD, 42 with DM-II, 63 with SLE and 20 normal controls (NC). RESULTS: The plasma level of sEPCR in patients with DM was higher than that in NC group (P < 0.05). The sEPCR level in SLE patients with thrombosis and non-thrombosis are higher than that in NC group (P < 0.005 and P < 0.001, respectively). There is no significant difference between the two groups of SLE (P > 0.05), and between the CHD and NC groups. CONCLUSION: sEPCR level increased in DM and SLE, it seems related to the process of inflammatory reaction and damage of endothelial cell.

Adolescent↗
PubMed2000

[Lymphocyte subsets analysis and glycosylphosphatidylinositol determination in patients with PNH].

OBJECTIVE: Absolute number of lymphocyte subsets and the proportion of glycosylphosphatidylinositol (GPI)-deficient clones among T, B and NK cells were determined. METHODS: By using multicolor flow cytometry with two and three color labelled monoclonal antibodies, absolute number of lymphocyte subsets and the proportion of CD(48), CD(55), CD(59)-deficient T, B and NK cells were analyzed. RESULTS: Lymphocyte subsets were found abnormal in all patients, low absolute number of natural killer cells and B cells were obvious. In most patients, GPI-deficient T, B and NK cells were detectable. The proportion of GPI-deficient clones in T, B and NK cells were 78.7%, 53.4% and 30.5%, respectively. There was significant difference among T, B and NK cells (P < 0.01). In two continuous remission cases, no GPI-deficient clones were found in erythrocytes, but there were GPI-deficient T and B cells, and in a 3 year remission there were still GPI-deficient NK cells. CONCLUSIONS: In PNH, similar to other blood cells, lymphocytes also had GPI deficiency, and GPI-deficient T and B cells could exist for a long time. NK cells of PNH patients are the predominant, GPI-deficiency cells with a high frequency (93.3%) and high proportion (78.7%).

Adult↗
PubMed2000

[Study on effect of spine surgery on gastric function and its efficacy of relevant treatments].

OBJECTIVE: To study the effect of spine surgery (SpS) on gastric function and the efficacy of relevant treatments. METHODS: Sixty patients in the spine surgery group, 20 patients in the extremity surgery (ES) group and 20 healthy subjects in the control group were observed. Electrogastrography (EGG) was used to observe gastroelectric activity before and after operation. Twenty patients among the SpS group were examined with barium meal under actinoscopy to observe the gastric peristaltic waves before and after operation. The SpS group was randomly subdivided into 3 groups, and treated by Xiangsha Yangwei pill (XSYW), moxibustion and motilium respectively. At the same time, the EGG of various groups was observed and the change of preoperative and postoperative EGG were compared. RESULTS: The gastroelectric rhythm of SpS group was remarkably abnormal, both frequency and amplitude of EGG were significantly different from the other two groups (P < 0.05, P < 0.01). The gastric peristaltic waves were reduced, and the emptying time was obviously prolonged. EGG was significantly improved after either of the three treatments statistically (P < 0.05), the effect of XSYW was the best, but in comparing with the other two, the difference was not significant (P > 0.05). CONCLUSION: SpS could change the gastroelectric rhythm to cause the gastric functional disorder and induce gastroparesis, EGG can get satisfactory results in the diagnosis of these illnesses. XSYW, moxibustion and motilium all have significant effects on the gastric function after SpS.

Acupuncture↗
PubMed2000

[Survey on children's dental fluorosis and fluoride content in urine after defluridation to improve drinking water].

The authors surveyed the dental fluorosis and fluoride content in urine of 8-12 years old children's in 1993 to 1999 for the evaluation of the efficiency to prevent endemic fluorosis after defluoridation to change drinking water source in Guangdong Province. Three villages: slight fluorosis area in Dazhai village, middle fluorosis area in Hupi village and severe fluorosis area in Anquan village in Fengshun County were surveyed. The results showed that the fluoride contents in drinking water were 1 mg/L (or less) in Anquan village, at the same time the prevalence of dental fluorosis and indexes of dental fluorosis were decreasing as changing water time. Fluoride contents in urine were normal. But in other two villages, the fluoride contents in drinking water exceeded 1 mg/L, therefore the children's prevalence rates and indexes of dental fluorosis were higher than the national standards. It is important to keep fluoride contents in drinking water under 1 mg/L for preventing endemic fluorosis by defluoridation to improve drinking water.

Child↗
PubMed2000

[Expression of c-fos mRNA following moderate lateral fluid percussion brain injury in rats].

This experiment was designed to study the expression of c-fos mRNA in brain following moderate lateral fluid percussion brain injury in rats and to observe the temporal pattern of its expressions following percussion. Male Sprague-Dawley rats were divided into normal control, sham operation control and injury groups. The rats of the injury group were subjected to moderate lateral fluid percussion injury (0.2 MPa). The injury group was then subdivided into 5 min, 15 min, 30 min, 1 h, 2 h groups according to the time elapsed after injury. The expression of c-fos mRNA was studied with reverse transcription polymerase chain reaction(RT-PCR) semi-quantitatively. c-fos mRNA in cortex and brain stem was expressed weakly in control groups. After 5 min of percussion, the expression of c-fos mRNA increased progressively and remained elevated up to 2 h after brain injury. This result suggested that the induction and expression of the c-fos mRNA in cortex and brain stem after fluid percussion brain injury were increased rapidly. The temporal pattern of induction in cortex was similar to that in brain stem.

Animals↗
PubMed2000

[A Carnobacterium-like organism isolated form a patient with multiple bacterial synergistic gangrene].

An atypical lactic-acid producing gram positive rod Y6 strain was studied in this report, which was isolated form clinic sample of a patient with multiple bacterial synergistic gangrene, and could not be identified by routine method. A 1.5 kb of 16S rDNA of Y6 strain was synthesized and sequenced. Comparative 16S rDNA sequence analyses revealed that stain Y6 is most closely related to the genus of Carnobacterium. The overall similarity value between Y6 strain and Carnobacterium species are 93% to 97%. The signature nucleotides in 16S rDNA primary sequence of strain Y6 and that of genus of Carnobacterium were identical. The biological features of Y6 strain are very similar to that of Carnobacterium, such as lactic acid as main end product of in PYG broth, no gas produced form fermentation of glucose, catalase negative, no motility. Data suggested that Y6 strain is very similar to the genus of Carnobacterium, of which no strain has been isolated form clinical sample so far. Based on the results obtained, we names Y6 strain as Carnobacterium-like organism.

Base Sequence↗
PubMed2000

[Purification and properties of beta-D-mannanase from Nocardioform actinomycetes].

After cultivating in liquid medium for 3 days, an extracellular endo-1,4-beta-D-mannanase (EC-3.2.1.78) from a Nocardioform actinomycetes strain NA3-540 was purified 137-fold to electrophoretic homogeneity by ammonium sulfate precipitation, 95% alcohol precipitation, CM-sephadex A-50, hydroxyaptite column chromatography, DEAE-cellulose anion-exchange column chromatography and Sephadex G-100 column chromatography. The enzyme had a molecular mass of 41 kDa(by SDS-PAGE) and 40 kD (by gel filtration), a pI of 4.8 (PAGEIEF) and carbohydrates content of 3.5%. The enzyme was optimally active at pH 8 and 75 degrees C and showed stability at pH range of 6.5 to 12 at the temperature below 60 degrees C. The amino acid composition analysis of the enzyme proved that there were large amount of Gly, Asp, Ala and Glu. The enzyme was strongly inhibited by Hg2+, Cu2+, Pb2+, Fe3+, Co2+, Zn2+ and partly inhibited by Mn2+, Fe2+, Ag+ and slightly inhibited by Na+, K+, Li+, Mg2+, Ca2+, Ba2+.

Actinomycetales↗
PubMed2000

[Production of monoclonal antibodies to broad bean wilt virus and application in virus detection].

Six hybridoma cell lines secretion monoclonal antibodies(MAbs) against broad bean wilt virus(BBWV) were produced by fusing mouse myeloma cells(SP 2/0) with spleen cells from BAL B/c immunized by the BBWV particles. The hybridoma cell lines secreted MAbs stably after cultured in vitro for 3 months or stored in liquid nitrogen and then revived for several times. The titres of ascitic fluids of six MAbs ranged from 1:256,000 to 1:640,000 when measured by indirect ELISA. In agarose gel immunodiffusion test, it showed that the six MAbs represented the same isotype of murine antibodies, IgG1. Six MAbs could detect 4 tested BBWV isolates, but didn't crossreact with other 5 plant viruses. The result of Western blot showed that all the six MAbs can react with the 44.7 kD large coat protein subunit of BBWV. This is the first report of production of MAbs against BBWV.

Animals↗
PubMed2000

[Studies on monoclonal antibodies of Magnaporthe grisea and its interfering effect on appressorium formation].

Selecting by indirect ELISA eleven hybridoma cell lines, secreting monoclonal antibodies (McAbs) against Magnaporthe grisea were produced by fusing mouse myeloma cells(SP2/0) with spleen cells from BALB/c mice immunized with the mixture of conidia, germ tubes and appressoria of M. grisea using 50% PEG. The result of IFTC test showed that four of McAbs, named as 2B4, 4A1, 1D1, and 2H4 specifically bound to the cell wall surface of the fungus; Western blotting revealed that 2B4, 4A1, 1D1 were recognized different protein antigens from the surface of conidia and germ tubes; These four McAbs could effectively interfere with the appressorium formation both on cellophane membrane and the surface of onion epidermis and inhibit the disease leaf lesions development in vitro test.

Animals↗
PubMed2000

[Artificial skin cultured in vitro].

Artificial skin is a kind of skin which by using tissue engineering techniques, a skin-like material was formed by seeding epithelium cells and dermis fibroblasts on a good biocompatible material after proliferation. The trauma restoration and reconstruction were achieved by transplanting the skin graft an skin defects after the artificial skin graft cultured in vitro. Artificial skin research is a new active field in tissue engineering. It has wonderful prospect for clinics to radically settle the problem of restoration of skin defects. The recent studies on the models, cultured methods and influenced factors for epithium culture, dermis culture and artificial skin in vitro were reviewed in this paper.

Bioartificial Organs↗