Biomedical subjects

J Wu

Publications and source records attributed to J Wu.

At least 505 records · Page 28Linked to original sources

PubMed2000

[Spiral CT features of hepatocellular caicinoma].

OBJECTIVE: To analyze the features of two-phase spiral scanning in 54 cases of hepatocellular carcinoma and to evaluate its usefulness in diagnosis and differential diagnosis. METHODS: Precontrast and postcontrast CT scans of the whole liver in all cases were carried out with GE-Hispeed Advantage spiral CT scanner. The arterial phase scanning was started at 25 sec after injection of contrast medium and the portal venous phase scanning at 70 sec. RESULTS: In the 54 cases examined, 72 lesions were found in the arterial phase. In 28 of these lesions, it was enhanced homogeneously, while in 40 lesions, the enhancement was not homogeneous. Supplying artery could be seen in 4 of them. The remaining 4 lesions had no enhancement. There were 62 lesiosns with low density in portal venous phase. Portal vein tumor thrombus was seen in 8 cases, and pseudocapsule was observed in 7 lesions. CONCLUSION: Two-phase spiral CT scanning is of value in the diagnosis and differential diagnosis of hepatocellular carcinoma.

Adult↗

PubMed2000

[Dendritic cell infiltration in lung adenocarcinoma and its effect on prognosis].

OBJECTIVE: To study the infiltration degree of dendritic cells in lung adenocarcinoma and its effect on prognosis. METHODS: S-100 protein was used as the specific marker for dendritic cells, and immunohistochemistry was employed to detect the distribution of dendritic cells in lung adenocarcinoma. RESULTS: Among 46 cases of lung adenocarcinoma, 19 were markedly infiltrated by dendritic cells, the five-year survival rate being 61%; 27 showed slight infiltration, the five-year survival rate being 21%. Among the 46 cases of adenocarcinoma, 26 were low differentiated adenocarcinoma, in which 10 were infiltrated markedly by dendritic cells, five-year survival rate being 57%; and 16 were slightly infiltrated, five-year survival rate being 16%. These data were evaluated with the log-rank test, which showed a significant difference between the markedly infiltrated group and the slightly infiltrated group. CONCLUSION: The prognosis of lung adenocarcinoma which markedly infiltrated by dendritic cells is better than that of slightly infiltrated by dendritic cells.

Adenocarcinoma↗

PubMed2000

[Genistein exerts multiple suppressive effects on human breast carcinoma cells].

OBJECTIVE: To study the suppressive effects of genistein on progression of breast carcinoma. METHODS: In vitro cell culture, animal studies, zymography, Northern and Western blot were used in this study. RESULTS: Genistein inhibited in vitro invasion of MCF-7 and MDA-MB-231 cells. This inhibition was associated with down-regulation of MMP-9 and up-regulation of TIMP-1. These effects were also demonstrated in vivo. Genistein inhibited growth of the breast cancer cells transplanted in nude mice. The growth inhibitory effect was associated with increased tumor cell apoptosis, and up-regulated p21WAF1/CIP1 expression. Furthermore, genistein inhibited angiogenesis as shown by decreased vessel density and decreased production and release of VEGF and TGF-beta 1. CONCLUSION: Genistein exerts multiple suppressive effects on breast carcinoma. It may help halt its progression.

Animals↗

PubMed2000

[Clinical implication of apoptosis of breast cancer induced by preoperative chemotherapy].

OBJECTIVE: To investigate whether apoptosis could be induced by preoperative chemotherapy in breast cancer patients, and determine if it is correlated with clinical response and patients' survival. METHODS: Apoptosis was assayed by in situ DNA end labeling and receptors for estrogen and progesterone were examined immunohistochemically. Comparison of the differences in apoptotic index (AI) and clinical response was made between patients received preoperative chemotherapy and those did not, using the one tailed Student's t-test. The Kaplan-Meier method was utilized to calculate relapse-free and overall survival time. Cox proportional hazards model was used to conduct multivariate analysis of prognostic factors and differences in overall survival and relapse-free survival after adjustment for other variables. RESULTS: Apoptosis was induced by preoperative chemotherapy, especially in lymph node positive patients. Post-chemotherapy AI correlated with better clinical response and increased patient survival, including relapse (disease) free survival and overall survival. CONCLUSION: The extent of apoptosis induced by preoperative chemotherapy of primary breast cancer may possibly predict an individual patient's overall response to treatment.

Adult↗

PubMed2000

[Effects of hexamethylene bisacetamide on in vitro adhesion, motility and invasion of human mucoepidermoid carcinoma cell line MEC-1].

OBJECTIVE: To investigate the effects of hexamethylene bisacetamide (HMBA) on the in vitro adhesion, motility and invasion of human mucoepidermoid carcinoma cell line MEC-1. METHODS: MEC-1 cells were induced with 2 mmol/L of HMBA in vitro, the adhesion of MEC-1 cells to the reconstituted basement membrane Matrigel was investigated with MTT colorimetric assay; the motility and invasion of MEC-1 cells were assayed using biopore membrane Millicell-PCF inserts culture system. RESULTS: The adhesion of MEC-1 cells treated with HMBA for 30 min or 90 min reduced by 26.47% and 31.13%, respectively; the motility and invasion of MEC-1 cells treated with HMBA reduced by 63.49% and 67.65%, respectively. CONCLUSIONS: HMBA has anti-invasion effects on human mucoepidermoid carcinoma cells in vitro.

Acetamides↗

PubMed2000

[Effect of xiaoqinglong decoction drug-serum on degranulation and histamine releasing of peritoneal mastocyte in sensitized rats].

OBJECTIVE: To study the mechanism of anti-allergic effect of Xiaoqinglong Decoction (XQLD). METHODS: The influence of XQLD drug-serum frozen powder on degranulation and histamine releasing of peritoneal mastocyte in sensitized rats was examined by morphologic and fluorimetric method. RESULTS: The drug-serum prepared by serum obtained 2 hours after medication for 15 min has obvious inhibitory effect on degranulation and histamine releasing of peritoneal mastocyte which showed a basically unified time-effect relationship. CONCLUSION: The anti-allergic effect of XQLD is related with the allergic medium (histamine) inhibition of releasing of peritoneal mastocyte.

Animals↗

PubMed2000

[Effect of reinforcing qi and moistening intestine oral liquid on anorectal manometry of asthenia type constipation patients].

OBJECTIVE: To study the change of anorectal manometry in asthenia type constipation patients and effect of reinforcing Qi and moistening intestine oral liquid (RQMI) on it. METHODS: The fotal of 135 cases were divided into healthy group, RQMI treated group, Maren pill (MRP) treated group and prepulside (PPS) treated group, their anal maximal voluntary squeeze pressure, rectoanal contraction reflex, rectoanal inhibitory reflex, defecation reflex, rectal volume sensory threshold and rectal maximal tolerable volume were observed. RESULTS: The rectal sensory function of patients weakened obviously and anal sphincter reactivity reduced as compared with those of healthy person (P < 0.01), and both were improved by RQMI treatment (P < 0.05). CONCLUSION: RQMI is superior to MRP and prepulside in improving anorectal dynamic abnormality in constipation patient of asthenia type.

Adult↗

PubMed2000

[Experimental research of gene therapy for human gliomas with vascular endothelial growth factor(165) antisense RNA].

OBJECTIVE: To investigate the feasibility of gene therapy for human gliomas with vascular endothelial growth factor(165) (VEGF(165)) antisense RNA. METHODS: The eukaryotic expression vector of antisense VEGF(165) was constructed and identified. Then the vector was transfected into human glioma cells (SHG44). The biological characteristics and tumorigenesis of SHG44 cells before and after transfection were inspected and compared. The changes were detected by Western blot, immunohistochemistry, micrangium counting, electron microscopy and flow cytometry. RESULTS: The eukaryotic expression vector pcDNA-AVEGF(165) was successfully constructed and transfected into SHG44 glioma cells. The characterstics of the cells were not influenced by the expression of antisense gene. The capability of tumorigenesis and angiogenesis of the transfected cells in nude mices was greatly reduced (tumour end volume, experiment group 212 mm(3); control group 7 897 mm(3); P < 0.01; Blood vessel counting: experiment group, 5.50; control group 11.22; P < 0.01 ). CONCLUSION: The angiogenesis and tumor growth of human gliomas are effectively inhibited by VEGF(165) antisense RNA. This experiment lays a foundation for solid tumor-specific gene therapy.

Animals↗

PubMed2000

[The experimental research of the rhBMP-2-induced apoptosis in human glioma cells].

OBJECTIVE: To investigate the effects of recombinant human bone morphogenetic protein (rhBMP-2) on the biological characteristics of human glioma cells (SHG44) in vivo and in vitro. METHODS: The growth curve of SHG44 cells was ploted with or without rhBMP-2. The proliferation quantity of SHG44 cells with rhBMP-2 was determined by MTT method. The cell cycle, ultrastructure and DNA fragments of SHG44 cells were detected respectively by flow cytometry (FCM), electron microscope and agarose gel electrophoresis. Tumor growth state of SHG44 gliomas, which grew under the skin of nude mice, was observed with local injection of rhBMP-2. RESULTS: FCM showed that rhBMP-2 inhibited the growth of SHG44 cell in vitro and its effect depended on dosage. DNA content of SHG44 cells in G1 phase was obviously increased (5 mg/L group, 77.4%; control group, 55.2%, P < 0.05) and the content of its S phase was reduced (5 mg/L group, 15.2%; control group, 33.3%, P < 0.01). The apoptosis spike (12.3%) occurred in the 5 mg/L group. The nucleolus chromatin condensation, bound of aggregation and caryorrhexis were observed under the electron microscope and the apoptotic cells appeared. DNA electrophoresis showed a ladder strap (DNA ladder). SHG44 glioma growth was relatively slower and its volume was smaller with the biquadratic local injection of rhBMP-2 (tumor end volume in average: experimental group 894 mm(3) +/- 145 mm(3), control group 5 163 mm(3) +/- 1 028 mm(3), P < 0.01 CONCLUSION: rhBMP-2 can suppress the proliferation of SHG44 gliomas and induce its apoptosisN:

Animals↗

PubMed2000

[Correlation between epidermal growth factor receptor messenger RNA expression and the onset of labor].

OBJECTIVE: To study the correlation between epidermal growth factor receptor messenger RNA expression and the onset of labor. METHOD: Semiquantitative reverse transcriptase polymerase chain reaction, using GAPDH as internal standard, was applied to determine the levels of epidermal growth factor receptor messenger RNA in human myometrium (n = 20) and fetal membranes (n = 20) before and during labor. RESULTS: In myometrium, epidermal growth factor receptor messenger RNA increased from 0.91 +/- 0.32 before labor to 1.33 +/- 0.23 after the onset of labor (P < 0.05). In fetal membranes, epidermal growth factor receptor messenger RNA increased from 1.05 +/- 0.30 before labor to 1.65 +/- 0.71 after the onset of labor (P < 0.05). CONCLUSION: The study indicates it is Up-regulation of epidermal growth factor receptors messenger RNA expression in myometrium and fetal membrane before and after the onset of labor.

Amnion↗

PubMed2000

[Ultrasonically guided radio-frequency ablation of liver tumors with a clustered electrode: a report of 100 cases].

OBJECTIVE: To evaluate the efficacy of ultrasonically guided radio-frequency (RF) thermal ablation of liver tumors with a clustered electrode. METHODS: Clinical records of 100 consecutive patients who underwent RF tissue ablation 120 times were analysed. Image data, tumor size, clinic manifestation, and AFP value before and after the procedure were compared. RESULTS: A hundred cases at 10 days, thirty at 30 days, ten at 60 days after the procedure were subjected to color ultrasonography and CT scans, respectively. The tumor size decreased by 20% at 10 days in 70% patients, decreased by 30% at 30 days in 80% patients, decreased by 50% at 60 days in 3 patients. AFP value decreased in 50% of patients, no operative death was noted, and the rate of operative complication was 23% after ablation. CONCLUSION: Ultrasound guided RF thermal ablation of hepatic tumors is safe, applicable and effective.

Adolescent↗

PubMed2000

[Microsurgical treatment of cranial base meningioma].

OBJECTIVE: To improve the total removal rate of cranial base meningiomas, and to reduce the mortality and morbidity. METHODS: 103 patients with cranial base meningiomas were treated by microsurgery. Tumors of different position were resected by variant transcranial base approaches and the operative methods. RESULTS: and complications were analyzed retrospectively. RESULTS: Total removal of Simpson's 1 to 2 grade was performed in 63 patients, subtotal removal in 31 and partial removal in 9. Good postoperative recovery was noted in 75 patients, skull nerve paralysis in 24 and death 4. CONCLUSION: Using skull base microsurgery to resect cranial base meningiomas could shorten the distance of surgical approach and lessen the damage to brain.

Adolescent↗

PubMed2000

[Effect of transforming growth factor - beta1 on expression of CD69(+) and CD25(+) T cell induced by allo - corneal epithelial cells in vitro].

OBJECTIVE: To study the effect of transforming growth factor - beta(1) (TGF-beta1) on changes of CD(69)(+) and CD(25)(+) T cell population induced by allo - corneal epithelial cells in culture. METHOD: The expression of CD(69)(+) and CD(25)(+) in peripheral T cells was identified by using indirect immune fluorescence flow cytometry analysis system. RESULT: The expression of CD(69)(+) and CD(25)(+) in peripheral T cells was significantly increased after peripheral blood had been cultured with allo - corneal epithelial cells for 3 hours However, the expression of CD(69)(+) was significantly decreased while TGF - beta(1) was added to the culture. CONCLUSION: TGF - beta(1) significantly inhibits the expression of CD(69)(+) and CD(25)(+) in peripheral T cells induced by allo-corneal epithelial cells in vitro.

Antigens, CD↗

PubMed2000

[Apoptosis and related gene expression in lacrimal gland of cases with Sjögren's syndrome].

OBJECTIVE: To assess the roles of apoptosis and expression of the related genes in lacrimal glandular destruction of patients with Sjögren's syndrome (SS). METHODS: Small pieces of palpebral lobes of lacrimal glands were obtained from 12 patients (10 with primary Sjögren's syndrome, 2 with secondary Sjögren's syndrome) and 7 normal controls. They had been investigated by the in situ end labeling and immunohistochemical staining to detect the apoptotic cells and the expression of Fas, FasL, bcl-2 and Bax. RESULTS: The number of epithelial apoptotic cells in lacrimal glands of patients with SS was significantly higher than that in normal glands, and SS epithelial cells showed increased expression of Fas, FasL and Bax. There was significant positive correlation between the number of the apoptotic cells and that of the cells expressing Fas, FasL and Bax, respectively, and there was significant negative correlation between the number of the apoptotic cells and that of the cells expressing bcl-2. CONCLUSIONS: The apoptosis of the epithelial cells in the lacrimal gland may be one of the mechanisms leading to the glandular destruction found in SS. In SS lacrimal glands, the expression of Fas, FasL and Bax may promote apoptosis, and the expression of bcl-2 may inhibit apoptosis.

Adolescent↗

PubMed2000

[Analysis on genetic pattern of familial type II diabetes mellitus].

OBJECTIVE: To analyse hereditary familial type II diabetes mellitus (type II DM), including its pattern and the transmitted expression on different sex of type II DM. METHODS: Familial analysis, segregation analysis, tests were used for multifactorial inheritance and threshold analysis. One hundred seventy-seven pedigrees of familial type II DM were studied. RESULTS: The heritability of familial type II DM was 143.06% +/- 3.3%. One dominant major gene might influence the genesis of type II DM. There were 214 nuclear families in 3 different mating types: U x U, U x A and A x A. The segregation ratios of U x U, U x A and A x A were 0.4703, 0.4838 and 0.6712 respectively. The results of tests for multifactorial inheritance and segregation analysis indicated that the genetic pattern of U x A and A x A nuclear families was not multifactorial inheritant and autosomal recessive (AR) but autosomal dominant (AD) inheritance. The genetic pattern of U x U nuclear families was neither AD nor AR inheritance. CONCLUSION: There was genetic heterogeneity in type II DM discovered with subgroupes of autosomal dominant inheritance in type II DM.

Adult↗

PubMed2000

[Analysis on the causes of burn among 17,339 patients].

OBJECTIVE: To find out the common causes of burn and to develop preventive measures to prevent or reduce the injury of burn. METHODS: Descriptive analysis was used to review the burn-cases in our Burn Department from 1970 to 1998 and to summarize the epidemic features and common causes of the injuries. RESULTS: From 1970 to 1998, a total number of 17 339 inpatients were seen in our Burn Department. According to the causes of injury, burn patients were categorized as heat-injured, electric-injured, chemical-injured, etc. Most patients were injured with heat, taking up 89.11% of the total patients (P < 0.01). Most of the heat-injured patients were scalded with hot water (49.31%, P < 0.01). Children being burnt, accounted for 32.82% of the total patients (P < 0.01), whom should not be ignored. CONCLUSION: Burn happens frequently in daily life, so the preventive methods should be taken seriously.

Adolescent↗

PubMed2000

[A study on relationship between plasma heat stress protein 70 and cytokine in patients with heat apoplexy].

OBJECTIVE: To study the change of levels of plasma heat stress protein 70 (HSP70) and cytokine and their interrelationship in pathogenicity of heat apoplexy. METHODS: Plasma HSP70 was determined with Western blot, and plasma interleukin-2 (IL-2), soluble interleukin-2 receptor (sIL-2R) and interleukin-6 (IL-6) were determined with enzyme-linked immunosorbent assay (ELISA). RESULTS: Plasma level of HSP70 was 4 211.2 +/- 1,286.2 (integral optical density) in average in the patients of heat apoplexy group, 4,137.8 +/- 1, 207.5 in those of severe heat apoplexy group, and 6,043.5 +/- 1,354.8 in control group, with very significant difference (P < 0.01). Plasma IL-2 level was (64.3 +/- 32.3) pg/ml and (200.0 +/- 50.0) pg/ml and sIL-2R (54.9 +/- 33.3) U/ml and (167.5 +/- 89.0) U/ml in the patients with heat apoplexy and in controls, respectively, with very significant difference. But, there was no significant difference in IL-6 between varied groups. CONCLUSIONS: The above-mentioned results suggest that establishment of heat acclimatization correlates to the interaction of network of neuroendocrine-cytokine-HSP, in which decrease in plasma IL-2 was one of the important factors causing reduction of HSP70 expression. Balance of network regulation was broken by pathological factors, finally resulting in heat apoplexy.

Adolescent↗

PubMed2000

[Preliminary study on relationship between antibody against heat stress protein 70 and hypertension].

OBJECTIVE: To preliminarily explore the relationship between main antibody against heat stress protein 70 (anti-HSP70) and hypertension. METHODS: Serum anti-HSP70 was determined with Western blotting technique in hypertensive and normotensive groups. Logistic regression model was used to analyze the relationship between anti-HSP70 and hypertension. RESULTS: It was found that level of antibody against the main heat shock protein 70 was significantly higher in patients with hypertension (based on criteria of 160/95 mm Hg, 1 mm Hg = 0.133 kPa) than in controls, after adjustment for the generally recognized risk factors for hypertension (such as age, sex, length of employment, body mass index, job, et al.). There still was stronger association between anti-HSP70 and hypertension (based on criteria of 140/90 mm Hg), but its strength of association was weaker than the former. CONCLUSIONS: Anti-HSP70 may potentially serve as a serum biomarker to assess hypertension, or anti-HSP70 itself may be involved in the development of hypertension.

Autoantibodies↗