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Biomedical subjects

J Wu

Publications and source records attributed to J Wu.

At least 343 records · Page 19Linked to original sources
PubMed2001

[A survey on contraceptive knowledge, attitude and behavior among never-married young women who are seeking pregnancy termination in Beijing].

OBJECTIVE: To identify contraceptive knowledge, attitude, behavior and its determinants among never-married young women who have unwanted pregnancy in Beijing. METHODS: A cross-sectional study under adoption of Lawrence "Procede-Proceed" model, was conducted in China in 1999. Three hundred and six unmarried young women aged 18 to 24, requesting for pregnancy termination were interviewed in person. RESULTS: Findings of this study indicated that only one-eighth (13%) of the young women insisted on contraceptive use every time, and almost an equal proportion (26.4%, 26.8% respectively) occasionally or never using contraceptives. Among 224 women who ever used contraceptives during the past 12 months, condom (49%) ranked the first place followed by withdrawal (27.7%) and the rhythm method (15.6%). One of the most important reasons, cited by 73 percent of women who had never used contraceptives, was that they were not aware of the risk of pregnancy when engaging in sexual activities. The results of logistic regression analysis revealed that knowledge about contraception, boyfriend's approval of contraceptive use, perceived the risk of getting pregnancy, perceived availability of contraceptive service and discussion of contraception with boyfriend were important indicators of young women's behavior on contraceptive use. CONCLUSION: These results indicated an urgent need to develop sex education about contraception among young women and men, through enhancing perception about risk of unwanted pregnancy and complications of induced abortion among young women, and to promote men's co-operation and participation in contraceptive use as well as strengthening communication on contraception between young women and their partners.

Abortion, Induced↗
PubMed2001

[A study on effect of basic fibroblast growth factor on human limbal stem cell proliferation cultured in low calcium medium].

OBJECTIVE: To study how to obtain relatively pure stem cells and the effects of bFGF on the human limbal stem cell proliferation in order to set up the foundation of cultured limbal stem cells for treatment of ocular surface and tear diseases. METHODS: Limbal button inoculation was used in low calcium culture. Monoclonal antibody AE5 was used to identify the stem cells. To examine the effects of bFGF (1 - 100 ng/ml) on stem cell proliferation, the technique of digital camera and computer image analysis system were used. RESULTS: Cultured cells were mostly corneal stem cells. This was the first time to combine the method of obtaining basal cell layer and culture with low calcium medium, which could harvest relatively pure and undifferentiated stem cells in localization and nature. The basic FGF (1 - 100 ng/ml) promoted cultured stem cells to proliferate significantly (P < 0.001). CONCLUSIONS: Limbal stem culture provides resources of stem cells. It is very useful in treatment of ocular surface and tear diseases. The basic FGF plays an important role in proliferation of stem cells in graft. Computer image analysis system is an effective and simple way for evaluating the proliferation of a primary culture.

Calcium↗
PubMed2001

[The difference of intracellular cytokines produced by different lymphocyte subsets of cord blood and adult peripheral blood].

OBJECTIVE: To explore the difference of intracellular cytokines between cord blood and adult peripheral blood and elucidate its significance for GVHD. METHOD: Different lymphocyte subsets were categorized in cord and adult peripheral blood by monoclonal antibodies with 3 color fluorescence using flow cytometry. The IL-2, TNF-alpha and IFN-gamma produced by various lymphocyte subsets were compared between the two groups. RESULT: (1) In cord blood, dominant T cells were CD(4)(+)CD(45RA)(+), while the major T cells were both CD(4)(+)CD(45RO)(+) and CD(8)(+)CD(45RO)(+) in adult peripheral blood. (2) The quantities of IL-2, TNF-alpha and IFN-gamma produced by cord blood lymphocytes were lower than that of adult peripheral blood lymphocytes. Moreover, the majority of cells that produced cytokines in the cord blood were CD(4)(+)CD(45RA)(+), but in adult peripheral blood were mainly CD(4)(+)CD(45RO)(+) and CD(8)(+)CD(45RO)(+). CONCLUSION: As compared with adult peripheral blood, the cord blood not only possessed different proportion of lymphocyte subsets, but also significant low level of cytokines. The differences of various lymphocyte subset proportions and lower level of cytokines in cord blood probably related to the lower incidence of GVHD in cord blood stem cell transplantation.

Adult↗
PubMed2001

[Changes serum level of tumor specific growth factor and their clinical significance in mechanical trauma patients].

OBJECTIVE: To investigate the post-traumatic changes in the serum level of tumor specific growth factor (TSGF) and their relationship with Injury Severity Score and to inquire into the possible mechanism and clinical significance. METHODS: Fifty patients with mechanical trauma were eligible and included. According to the standard of Injury Severity Score (ISS), the patients were divided into there groups: mild degree group, (ISS < or = 16, n = 31) severe degree group (16 < ISS < 25, n = 13) extreme severe degree group (ISS > or = 25, n = 6). The blood samples of all patients were obtained within 24 hours, and those of 25 dynamically observed patients were obtained on days 3, 5, 7, 9 and 12. RESULTS: The serum level of TSGF was significantly higher in patients with trauma in different groups (P < 0.05). The dynamical observation showed that the index demonstrated curve changes with increase in early stage and decrease later. the climax of TSGF appeared on 7 day in all 3 groups. The TSGF serum levels in extremely severe degree and severe degree groups > mild degree group on days 5, 7, 9 and 12 day (P < 0.05). CONCLUSION: TSGF has relationship with the inflammatory response and recovery of trauma.

Adult↗
PubMed2001

[Factors involved in intelligent development of children with cleft lip and palate].

OBJECTIVE: The objective of this study was to investigate the possible factors involved in intelligent development of children with cleft Lip and palate. METHODS: The intelligence quotient (IQ) and developmental quotient (DQ) of 152 children with cleft lip and palate under 14 years old were conducted, and the control group was made up of 80 healthy children. The considered factors included degrees of deformation, situation of perinatal period, way of feeding, psychological conditions of the children, general situation of their parents and education of these children. The data were statistically analyzed and compared. RESULTS: The IQ value of these children generally was normal, but the mean value was still lower than that of the control group (P < 0.01). The mean IQ value of the group of children with only cleft lip or cleft palate was higher than that of the group with both cleft lip and cleft palate, and the mean IQ value of these children with II degree deformity was higher than that of children with III degree deformity. The mean IQ value of these children feed with mother's milk was higher than that of those feed with artificial milk. These children more than 5 years old were disturbed psychologically, and their parents were also with pressure. CONCLUSION: The intelligent development of children with cleft lip and palate is affected by many factors, including types and degrees of deformity, feeding ways, illnesses and social psychological factors in different periods of development. Therefore, it is very important to take steps before 5 years old, including correctly feeding, repairing the deformity as early as possible, preventing the illnesses, necessary psychological treatment, improving social environment.

Adolescent↗
PubMed2001

[Reconstruction of post traumatic auricle partial defect by post-auricle flap schlepping muscle root].

OBJECTIVE: To study the method of repairing post-traumatic auricle partial defect. METHOD: 11 cases of post-traumatic auricle partial defect had been repaired by post-auricle flap schlepping muscle root in our hospital, the follow-up period from 3 months to 1 year. RESULT: It shows excellent shape. The flap necrosis hadn't been happened. CONCLUSION: Advantages of this procedure were simple to manage, texture match and good skin color. The donor scar was unconscious. It is a choice for treating post-traumatic auricle partial defect.

Adolescent↗
PubMed2001

[Realization of voice coil motor control with H8/338 micro-controller unit in left ventricular assist pump].

In this paper, we present a PID algorithm with velocity project of voice coil motor controlled on the base of H8/338MCU, and we find a driving part, which it is smaller, lighter and more reliable, for the left ventricular assist device. By means of the simulated experiment on the system, the result is satisfying. So we can say that it is a good help for the realization of LVAD.

Algorithms↗
PubMed2001

[A clinical evaluation on reconstruction in extended partial laryngectomy for transglottic cancer].

OBJECTIVE: Transglottic cancer has been a definite indication for total laryngectomy in decades, compromising function of the larynx. The purpose of this study is to present a new radical surgery of extended partial laryngectomy and reconstruction to restore the essential function of larynx. METHODS: Forty-one cases of transglottic cancer were treated by extended vertical laryngectomy (26/41), extended frontolateral laryngectomy (5/41) and subtotal laryngectomy (10/41); the defects of larynx were reconstructed by rotary door myocutaneous flap in twenty-seven cases, by sternohyoideus flap in seven cases, by osteomuscular flap in five cases and by sternocleidomastoid flap in two cases. RESULTS: The 3 and 5 year survival rates were 85.7% (30/35) and 74.1% (20/27) respectively, those for stage III were 84.6% (22/26) and 76.2% (16/21), for stage IV were 3/4 and 1/2 respectively. All cases resumed acceptable voice, 92.7% (38/41) and enjoyed satisfactory phonation. The over-all decannulation rate was 87.8% (36/41), those with rotary door myocutaneous flap was 96.3% (26/27), with sternohyoideus flap was 5/7, with osteomuscular flap was 4/5 and with sternocleidomastoid flap was 1/2. All patients had normal swallow function. CONCLUSION: Extended partial laryngectomy is a kind of radical operation in selected cases of transglottic cancer. The method of reconstruction with rotary door myocutaneous flap can improve curative effect and the essential functions of the larynx.

Adult↗
PubMed2001

[Clinical evaluation on second stage reconstruction for laryngostenosis after partial laryngectomy for laryngeal cancer patients].

OBJECTIVE: To present a new method of decannulation for laryngostenosis patients after partial laryngectomy and extended partial laryngectomy, and restoration of the essential functions of larynx and normal neck appearance. METHODS: Nineteen cases of laryngostenosis after partial laryngectomy and extended partial laryngectomy were treated with second stage reconstruction. The patients had been treated by vertical laryngectomy (6/19) using extended vertical laryngectomy (11/19) and frontolateral laryngectomy (2/19). Among these patients, second stage reconstruction of larynx was performed by using rotary door myocutaneous flap (17/19). Stemohyoideus flap (1/19) and sternocleidomastoid flap (1/19). RESULTS: Three and five year-survival rates were 91.7% (11/12) and 3/5, respectively. Over-all decannulation rate was 84.2% (16/19), but 94.1% (16/17) in patients with rotary door myocutaneous flap and 0% (0/2) in both patients with sternohyoideus flap and sternocleidomastoid flap. There were 3 decannulation failures (15.8%). All patients resumed acceptable voice, 94.7% (18/19) enjoyed satisfactory phonation, but 5.3% (1/19) showed severe hoarseness. All except 2 patients returned normal swallow function. The latter 2 patients experienced mild abnormal swallow during eating fluid food in early stage of surgery, but had normal swallow function after 1-2 weeks. CONCLUSION: Functional laryngectomy is a radical operation in selected cases with advanced laryngeal cancer. The second stage reconstruction with bi-pedical rotary door myocutaneous flaps can help decannulation in patients who developed laryngostenosis after partial laryngectomy and extended partial laryngectomy, and restore the essential function of larynx and normal neck appearance.

Adult↗
PubMed2001

[Propagation of hepatitis E virus in several cell lines including human embryo lung diploid cell KMB17].

OBJECTIVE: To investigate the hepatitis E virus (HEV) sensitive cells and its tissue culture conditions. METHODS: The HEV from dejecta supernatant of patients with acute hepatitis E was amplified and activated by passaged in Rhesus. Then, the positive dejecta samples of infected monkeys were dealt with super-centrifugation and virus for culture was obtained. Various human-derived (including KMB17, A549, BEL7402, and Hela) and non-human primates derived cells (Vero) were inoculated with HEV. Sensitivity of cells to HEV was measured by CPE (cytopathic effect), RT-PCR and immunofluorescence. RESULTS: CPE in KMB17, A549 and BEL7402 cells appeared during 7-9 days, meanwhile, cells shelled during 11-13 days on the first filial generation. The existence of HEV genome +RNA and replicated -RNA was still detectable by RT-PCR after the tenth filial generation. Neither CPE nor amplification of HEV genome RNA could be detected in Hela and Vero cells after the second to fourth filial generation. HEV could also be detected from inoculated KMB17 cells by immunofluorescence and RT-PCR. CONCLUSIONS: It indicates that KMB17, A549 and BEL7402 cells are sensitive to HEV under the experimental culture conditions, while Hela and Vero cells are insensitive. Tissue culture system of HEV in certain filial generation is established.

Animals↗
PubMed2001

Association between low-density lipoprotein receptor-related protein gene, butyrylcholinesterase gene and Alzheimer' s disease in Chinese.

OBJECTIVE: To research the relations between low-density lipoprotein receptor-related protein gene (LRP) polymorphism, butyrylcholinesterase gene (BchE) polymorphism and Alzheimer's disease (AD) in Chinese. METHODS: The gene polymorphisms of LRP and BchE were genotyped in 38 AD cases and 40 controls with polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) methods. AD groups were classified according to the LRP C/C genotype and compared with matched controls. RESULTS: AD group had higher frequencies of C/C homozygote (81.69% vs 60.0%, P < 0.05) and of C allele (89.5% vs 76.3%, P < 0.05), with no significant difference between any of these LRP genotypes classified AD groups and their respective control groups. CONCLUSIONS: A positive correlation was found between LRP gene polymorphism and AD, but not between BchE gene polymorphism and AD in Chinese AD cases.

Aged↗
PubMed2001

[Uncommon fluorescent properties of nano-structured terbium (III)-phthalic acid complex in polyvinylpyrrolidone].

Nano-sized terbium(III)-phthalic acid complex was prepared in polyvinylpyrrolidone. It's fluorescent spectrum has been investigated, compared to that of common terbium(III)-phthalic acid complex and terbium(III)-polyvinylpyrrolidone complex. The results show that fluorescent characterizations of Nano-sized terbium(III)-phthalic acid complex is obviously different from that of common terbium(III)-phthalic acid complex and terbium(III)-polyvinylpyrrolidone. The relative intensity and half-height width of main emission peaks are obviously varied, two splitting peaks are displayed.

Chelating Agents↗
PubMed2001

[Determination of 10 elements in the 12 kinds of Chinese traditional medicines by AAS].

The 10 kinds of inorganic elements were determined in 12 kinds of Chinese traditional medicines. The relation showed that there were numerous inorganic elements in the traditional chinese medicines. The relation between these inorganic elements and the efficacies of these medicines was studied and the efficacies of these Chinese traditional medicines were discussed. The efficacies of these traditional medicines were greatly influenced by the content of inorganic elements. To guarantee the efficacies of these traditional medicines, the reasonable control lines concerned with the content of inorganic elements shall be established and the lines will be discussed in the future.

Calcium↗
PubMed2001

[Green electroluminescence generated from a new rare earth complex: Tb(asprin)3phen].

Pure Green and narrow bandwidth emission from organic electroluminescent device was presented by using a new rare earth complex Tb(asprin)3phen as emissive layer. The structure of the device was ITO/PVK:Tb(asprin)3phen/Al, where PVK was used to improve the film-forming ability and conductivity of Tb(asprin)3phen. The electroluminescent property of the device was studied. It proved that this new kind of rare earth complex has excellent optoluminescent and electroluminescent properties. The electroluminescent mechanism of the device was proposed by measuring and analyzing the emission and excitation spectra of the emissive layer. The excitation spectrum of Tb(asprin)3phen-dispersed PVK film was very similar to that of the PVK. We proposed that the excited carriers of PVK and Tb(asprin)3phen were captured by Tb3+ and light was emitted when the electrons and holes recombined at Tb3+.

Chelating Agents↗
PubMed2001

[Study on calcium-cholate-phosphate ternary complexes by FTIR spectroscopy].

The aim of this paper was to examine the calcium-cholate-phosphate tenary complexes by FTIR spectroscopy. The results indicated that the mole ratio of cholate to phosphate were 1:3 and 1:4 in ternary complexes respectively when the initial mole ratio of cholate to phosphate were 3:1 and 1.5:1 in reaction. The FTIR spectroscopic results demonstrated that in the ternary complexes Ca2+ was coordinated with O in both COO- and PO(4)3- groups.

Calcium↗
PubMed2001

[FTIR study of transition metal 2-acrylamido-2-methyl-1-propanesulfonates].

Compounds of transition metal ion (Cu2+, Co2+, Ni2+) 2-acrylamidomethyl-2-methyl-1-propanesulfonates were studied by FTIR and X-ray powder diffraction techniques. The FTIR spectra of Co and Ni compound are similar but are different from that of Cu compound. The results of X-ray diffraction indicated that the crystal structure of Co and Ni compound are different.

Acrylamides↗
PubMed2001

[Effect of crystallization and branch on fine structure of orthorhombic in polyethylene].

The CH2 rocking bands are used as a probe to monitor the crystalline behavior of polyethylene segments in a series of poly (ethylene-octene) copolymers. High resolution and cryogenic FTIR spectra reveal that different composition and/or crystalline condition of the copolymers result in significant variation on the CH2 rocking bands including the change in peak position, band width and A730/A720. We conclude that side chain in the copolymer can influence the structure of the orthorhombic polyethylene crystallite although they can not enter the crystal lattice.

Crystallization↗