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Biomedical subjects

J Wolf

Publications and source records attributed to J Wolf.

At least 271 records · Page 15Linked to original sources
PubMed1983

Rat serum albumin synthesis in variant rat hepatoma cells.

Variant subclones of the rat hepatoma cell line FU5-5 have been isolated that are altered in their production of rat serum albumin. Three of these variants, isolated in a random screening, have been categorized as high, intermediate, and low producers. They secrete albumin into the culture medium at different rates: 16, 1.7, and 0.3 microgram/mg cell protein/48 h. A fourth variant, isolated on the basis of altered morphology, secretes no detectable albumin. Unlike the albumin-producing variants, this null variant is also deficient in the level and inducibility of tyrosine aminotransferase activity. Albumin biosynthesis as determined in pulse-labeling experiments is affected similarly in the four variants, yielding albumin synthetic rates of 0.24, 0.035, 0.006, and less than 0.002% of total protein synthesis. The translatable albumin messenger RNA content in these variants was measured using a rabbit reticulocyte lysate system. The null variant contains no detectable mRNA, and the three quantitative variants contain levels of translatable albumin messenger RNA corresponding to 0.07, 0.03, and 0.005% of total stimulated polypeptide synthesis. The highest producing variant contains less translatable albumin mRNA than expected on the basis of cellular biosynthetic measurements, suggesting a translation efficiency difference in this clone. Cell hybrids constructed by fusing the high-producing clone and the null variant produce little or no albumin. This extinction indicates that the null variant contains a diffusible regulatory factor capable of decreasing albumin gene expression. The relatively stable and discrete heritable phenotypic changes exhibited by these clones may serve as a model for similar changes that occur during hepatic differentiation.

Animals↗
PubMed1983

The pharmacokinetics of temocillin in patients with normal and impaired renal function.

The pharmacokinetics of temocillin was studied in 16 subjects with varying degrees of renal functional impairment. The subjects were divided into three groups, depending on their creatinine clearance: Group A greater than 70 ml/min/1.73 m2; Group B 20-70ml/min/1.73 m2 and Group C less than 20 ml/min/1.72 m2. Following intravenous administration the total serum clearance of temocillin was reduced in patients with renal insufficiency. The distribution of temocillin into the tissues was not affected by renal dysfunction, apparent distribution volumes being 15.11, 16.01 and 13.81 in Groups A, B and C respectively. The change in clearance was reflected in an increased area under the serum concentration versus time curve from 418 mg h/l in Group A to 1301 mg h/l and 1553 mg h/l in Groups B and C respectively. The beta elimination half-life was also prolonged from 3.5 h in Group A 12.7 h in Group B and 17.5 h in Group C. The urinary excretion of temocillin was both delayed and reduced according to the extent of kidney damage. The reduction in temocillin clearance was proportional to the extent of reduction from normal of the clearance of creatinine. This relationship has been used to suggest dosage schedules for temocillin in patients with renal insufficiency.

Adolescent↗
PubMed1983

A reconsideration of the relation of ventricular enlargement to duration of illness in schizophrenia.

The size of the ventricles of 19 young hospitalized patients with chronic schizophrenia was compared with that of 29 same-age control subjects. The patients had a slightly but significantly larger bicaudate ratio. Analysis of these results in the context of previous studies of ventricular size and schizophrenia suggests that, contrary to current views, there is a direct relationship between size of ventricles and duration of schizophrenic illness. The authors argue that the conflicting results of different studies of ventricular size and schizophrenia can be reconciled by postulating that both clinical schizophrenia and ventricular enlargement are overt signs of an underlying neuronal degeneration that is hereditary and progressive.

Adult↗
PubMed1983

[The tendon test of the tibial nerve in Hansen's disease].

Seventeen patients, presenting Hansen's disease, were studied by the Tendon-test. Nine presented pathological answers and 4 did not answer at all, probably due to serious damage. So the amount of pathological answers reached to 76.5% (13 patients). On the other hand, the answer of two patients was considered within the limits and they were classified with the normal answering patients that reached to 23.5% (4 patients). In order to find out if the tibial nerves are also injured simultaneously wit the ulnar and the median ones, it is advisable to employ this method in a larger number of patients and in a different social-geographical environment.

Adult↗
PubMed1982

Gardner's stigmas in patients with familial adenomatosis coli.

Extracolonic lesions compatible with Gardner's syndrome were studied in 34 patients from 17 unrelated families with established familial adenomatosis coli. Thirteen patients (38 per cent) had the complete syndrome and 12 patients (35 per cent) a bisymptomatic syndrome. Bone lesions were found in 79 per cent of the patients examined, epidermoid cysts in 35 per cent, desmoid tumours in 18 per cent and dental changes in 18 per cent. One extracolonic manifestation of the triad in at least one affected family member was detected in 14 families (82 per cent). The findings provide additional evidence for the view that all cases of familial adenomatosis coli belong to a single genetic entity. The usually innocent extracolonic signs of Gardner's syndrome severe as valuable markers for early detection of asymptomatic family members at risk. Panoramic X-ray of the jaws seems to be especially suitable for this purpose.

Adolescent↗
PubMed1982

The measurement of total respiratory resistance in small children.

A new, simplified apparatus to determine total respiratory resistance (Rrs) was presented. This apparatus, which utilizes the principle of forced oscillation, is portable, relatively simple to operate and calibrate, and small children can be tested with no apparent difficulties. To investigate the usefulness of the apparatus, total respiratory resistance was measured in 101 healthy children, ages 3-5. Rrs was found to be the highest in the younger children and was decreased with increasing age. The greatest variability in Rrs was found among the youngest children. Linear regression equations for Rrs versus height, weight, and body surface area were established. The best correlation was found between Rrs and height (r = -.325; P less than 0.01) of the children. Utilizing the described apparatus, Rrs can be determined relatively easily without requiring special effort by the subject and is a useful tool to monitor the airways function of young patients.

Airway Resistance↗
PubMed1982

Quasistationary states in red cell suspensions with special regard to blood and red cell preservation.

A biophysical model of a red cell suspension which is considered as a closed two-compartment system is established. The model includes the main components of resuspending solutions for red cell preservation which are of biophysical importance. Knowing the composition of the resuspending solution and the mixture cell sediment/resuspending solution the relative cell volume, the cell pH, the pH of the medium, and the transmembrane potential can be calculated. The model is in good accordance with experimental findings (temperature dependence of suspension pH, depolarisation of cells by addition of sucrose or citrate to the outer medium, acidification of the medium by lactate production) and enables one to explain these observations to a certain degree of accuracy also quantitatively. Relations to biochemical aspects of red cell and blood preservation are shown.

Blood Preservation↗
PubMed1981

Rationale and application of beta-2-microglobulin measurements to detect acute transplant rejection.

Serum and urinary concentrations of beta 2-microglobulin were measured for the first 21 days after renal transplantation to aid in diagnosis of acute rejection. Criteria developed after study of 15 patients were applied to the entire group of 31 consecutive cases. 29 instances meeting our criteria were identified in 651 days at risk and were associated with a mean maximal increase of serum creatinine of 74.8%. beta 2-Microglobulin methods may make possible detection of what is now subclinical rejection. beta 2-Microglobulin methods, however, are an adjunct to, not a replacement for classical methods for detecting acute rejection.

Beta-Globulins↗