Search PubMed⌕ Search

Biomedical subjects

J Wolf

Publications and source records attributed to J Wolf.

At least 253 records · Page 14Linked to original sources
PubMed1987

Influence of N,N-dimethylaniline on the association of phenobarbital-induced cytochrome P-450 and NADPH-cytochrome c(P-450) reductase in a reconstituted rabbit liver microsomal enzyme system.

N,N-Dimethylaniline when added to reaction mixtures provokes deviation from Michaelis-Menten law of the interaction kinetics of NADPH-cytochrome c(P-450) reductase (NADPH:ferrihaemoprotein oxidoreductase, EC 1.6.2.4) with highly purified phenobarbital-induced rabbit liver microsomal cytochrome P-450 (P-450LM2). This phenomenon is not associated with the low-to-high spin transition in the iron-coordination sphere of the haemoprotein, as elicited by the arylamine. Substrate-triggered departure from linearity of the kinetics is abolished by inclusion into the assay media of p-chloromercuribenzoate, hinting at a vital role in the process of thiols. Similarly, the parabolic progress curve (nH = 1.7) is transformed to a straight line (nH = 1.01) when the N-terminal reductase-binding domain in the P-450LM2 molecule is selectively blocked through covalent attachment of fluorescein isothiocyanate (FITC); such a modification does not alter the affinity of the haemoprotein for the amine substrate. Steady-state fluorescence polarization measurements reveal that N,N-dimethylaniline perturbs the motional properties of the fluorophore-bearing reductase-binding region, suggesting the induction of a conformational change. Summarizing these results, the data possibly indicate N,N-dimethylaniline-induced cooperativity in the association of reductase with P-450LM2.

Aniline Compounds↗
PubMed1987

[Neopterin in the serum and urine in the differential diagnosis of disorders of kidney function following kidney transplantation].

In a period of 10 months neopterin in serum and urine was determined by radioimmunoassay in 33 renal allograft recipients treated with cyclosporin A. While in allograft rejections the highest neopterin concentrations were found in the serum, patients with viral infections after renal transplantation showed the most elevated concentrations in the urine. For early diagnosis of allograft rejection the ratio of neopterin clearance and serum-neopterin was the most significant criterion of the parameters measured in this study. Patients without complications during the follow-up showed slightly elevated and stable neopterin levels in serum and urine. The presented results indicate that neopterin is a useful parameter for the follow-up after renal allograft transplantation and for the diagnosis of immunological complications.

Adult↗
PubMed1987

Injury and physiological responses of Larrea tridentata (DC) Coville exposed in situ to sulphur dioxide.

The response of shrubs of Larrea tridentata (DEC) Coville (creosotebush) exposed to sulphur dioxide (SO(2)) was evaluated using in situ plants of the Majove Desert. Larrea was exposed to acute levels of 0.3 to 2.0 microl litre(-1) SO(2) for periods up to 13 days using field chambers or an open-air fumigation system. Plants exposed in the spring exhibited considerable leaf injury (necrosis and defoliation) when exposed to 2.0 microl litre(-1) SO(2), and in the autumn had leaf injury when exposed to >0.4microl litre(-1) SO(2). Injured plants had higher transpiration rates, less negative water pressure potentials, and/or lower photosynthetic rates than control plants. It is likely that Larrea would not be injured by the typically low SO(2) concentrations and dry environmental conditions of the Mojave Desert. However, if injury were to occur, it would be accompanied by changes in plant-water relations and photosynthesis, followed by recovery after the SO(2) stress was removed.

Journal Article↗
PubMed1987

Dental anomalies in familial adenomatous polyposis coli.

Forty-seven Danish and 50 Finnish patients with familial adenomatous polyposis coli (FPC) were studied by panoramic tomography (PTG) of the mandible, which showed dental abnormalities in 17% of the cases. Eleven patients (11%) had supernumerary teeth and/or compound osteomas, and nine patients (9%) had impacted permanent teeth. The frequency of the dental anomalies was statistically significantly higher in FPC patients than in the normal population; it is therefore concluded that this dental abnormality should be included in the list of extracolonic manifestations that may occur in any FPC patient. The frequency of dental anomalies was higher in Finnish than in Danish patients, probably owing to a higher frequency of extracolonic manifestations in the Finnish series.

Adenomatous Polyposis Coli↗
PubMed1987

An endoscopic comparison of the effects of etodolac, indomethacin, ibuprofen, naproxen, and placebo on the gastrointestinal mucosa.

To assess the effects of various nonsteroidal antiinflammatory drugs on gastroduodenal mucosa, 72 normal men were administered either etodolac, indomethacin, ibuprofen, naproxen, or placebo for 7 days. Gastric and duodenal mucosa were scored before and after treatment by direct endoscopy and evaluation of photographs taken during endoscopy. Subjects treated with indomethacin, ibuprofen, and naproxen had significantly worse direct gastric scores, and those treated with indomethacin and naproxen also had significantly worse direct duodenal scores, when compared with pre-treatment and placebo scores. Subjects given either of 2 doses of etodolac had gastric and duodenal scores comparable to pretreatment and placebo scores. The direct gastric scores of the etodolac groups were significantly better than the scores of subjects receiving indomethacin, ibuprofen and naproxen. Similar results were obtained when endoscopy photographs were scored.

Acetates↗
PubMed1987

Early graft function.

1. Most of the first rejections occurred between the first and third weeks after transplantation, with the peak at 7 days. 2. Average serum creatinine values of 2 mg/dl were achieved in 2 days for living-related donor transplants. Cadaver donor grafts reached an average of 4 mg/dl by the tenth day, but in patients without rejection serum creatinine values of almost 2 mg/dl were achieved. 3. Cadaver donor grafts with cold ischemia times less than 12 hours led to the best early serum creatinine values. Cold ischemia times up to 48 hours and possibly beyond led to slightly higher creatinine levels but did not produce damage in proportion to the cold ischemia time. 4. Patients who were sensitized to more than 50% of the panel showed evidence of sensitization by having higher serum creatinine levels from one week to 3 weeks. 5. Even in the first few days after transplantation with a cadaver donor, black recipients had a higher serum creatinine than white recipients. The donor's race did not have a marked effect. 6. CsA doses given to cadaver donor recipients were about 2-4 mg/kg higher than living-related donor transplants. Patients experiencing a rejection episode were found to have been treated with about 1 mg/kg more than those without rejections. The corresponding blood levels of CsA were higher in the cadaver donor grafts and those with rejections. 7. Early serum creatinine levels were very strongly correlated with the one-year graft survival rate. Even one day after transplantation, the difference between patients with the best and worst one-day serum creatinine levels was as much as 30% in the one-year graft survival rate. At 2 weeks, the group with the best serum creatinine had a one-year survival rate that was 50 percentage points higher than the worst creatinine group. At one month, the best serum creatinine group had a one-year survival rate that was about 80 percentage points higher than the worst serum creatinine group. 8. Patients with high CsA levels of over 400 ng/ml in the early one-month posttransplant period, had poorer one-year graft survival than those with lower levels. The optimum level appeared to be less than 100 ng/ml by the tenth day and about 100-200 ng/ml to the third week posttransplant.(ABSTRACT TRUNCATED AT 400 WORDS)

Creatinine↗
PubMed1987

[Intensified conventional insulin therapy in children and adolescents with type I diabetes].

Children and adolescents with type-I-diabetes are examined with regard to the influence of Intensified Conventional Insulin Therapy (ICT) on metabolic control, insulin requirement and weight development. On average the period of treatment with ICT lasted 10 months (SD: 3 months). The average insulin requirement under ICT slightly increased from 0.82 to 0.87 I.U./kg BW. The need for longer acting insulin decreased from 0.5 to 0.35 I.U./kg BW. Snacks were not necessary. On average the insulin-BE quotient increased to 2.4 I.U./BE in the morning, 1.9.I.U./BE at lunch time and 2.1 I.U./BE at night. Compared to a group being treated with Conventional Insulin Therapy the quality of metabolic control improved. Within the ICT group the part of well controlled patients rose from 10 to 30% whereas the part of poorly controlled patients decreased from 30 to 10%. Provided that Continuous subcutaneous insulin infusion pumps (CSII) cannot be applied, ICT represents the best form of therapy for well educated and highly motivated diabetics aged 12 years old or more.

Adolescent↗
PubMed1986

Development of a Stafne mandibular bone cavity. Report of a case.

The present study reports a case in which a clinically asymptomatic radiolucent change surrounded by a sclerotic wall developed below the second molar in the mandible of a middle-aged man. The change was diagnosed as a typical case of Stafne bone cavity. In the earlier literature, development of a mandibular bone cavity has been described in only three patients. The appearance of bone cavities in a mandible that has previously been found normal indicates that these defects are not congenital but developmental processes.

Adult↗
PubMed1986

Gardner's dento-maxillary stigmas in patients with familial adenomatosis coli.

Fifty patients with familial adenomatosis coli (FAC) studied by panoramic tomography (PTG) showed osteomatous jaw changes in 82% as compared with 10% in matched controls. Supernumerary teeth, compound odontomas and/or impacted teeth were seen in 30% of the patients compared with 4% of the controls. A correlation was found between dental abnormalities and the large number of osteomas. The results support the view that both osteomas of the jaws and dental abnormalities are features of FAC. PTG of the jaws may serve as a valuable tool for early detection of FAC by oral surgeons, at least in the case of the most striking changes (about 20%).

Adenomatous Polyposis Coli↗
PubMed1986

Osteosarcoma of the mandible: analysis of nine cases.

A total of 16 cases of mandibular osteosarcoma were diagnosed histologically in Finland and reported to the Finnish Cancer Registry between 1953 and 1983. Review of the histologic slides confirmed the diagnosis in only nine cases. The overall five-year survival rate was 44%. Three patients who were free of clinical disease at the end of the follow-up period (mean, 9.9 years) either had tumors of low-grade malignancy (two cases) or had undergone prompt radical surgery (one case). The importance of radiography in the diagnosis of mandibular osteosarcoma is stressed, because the widening of the periodontal membrane spaces, the sunray effect, and the widening of the mandibular canal are almost pathognomonic for osteosarcoma.

Adult↗
PubMed1985

The transcervical cardiac catheter: its transverse pretracheal placement in the treatment of cardiac catheter complications with ventriculo-atrial shunts in infantile hydrocephalus.

In 40 hydrocephalic children with ventriculo-atrial shunts 58 operative revisions of the cardiac catheter were evaluated. The review of the patients with cardiac catheter complications showed that the majority (68%) had undergone the primary shunting procedure as newborn or as prematurely born within the first 6 months of life. Most frequently the revisions had become necessary because of obstruction of the cardiac catheter at its distal end (22 times) and the slipping of the catheter from the valve (21 times). Other revisions were carried out for too long (2 times) or too short (once) cardiac catheter, rupture (5 times), kinking or coiling (7 times) of the catheter. The transcervical cardiac catheter, which was given particular attention, used for insertion in revisions for venous obliteration on the side of the previous catheter placement, was performed in 11 patients. It yielded good results in respect of practicability and prognosis and can be recommended as a useful method for shunt revision in venous obstruction.

Cerebrospinal Fluid Shunts↗
PubMed1984

The role of lipid peroxidation in the N-oxidation of 4-chloroaniline.

Irradiation with u.v. light of aerobic aqueous media containing both rabbit liver microsomal fraction and 4-chloroaniline results in N-oxidation of the arylamine. The reaction is severely blocked by exhaustive extraction with organic solvents of the microsomal membranes to remove lipids. Further, scavengers of OH. and O2.-impair the photochemical process. These findings suggest that the observed phenomenon may be closely associated with light-induced lipid peroxidation. Indeed, N-oxidation of 4-chloroaniline is fully preserved when either phospholipid liposomes or dispersed linoleic acid substitute for intact microsomal fraction. Co-oxidation of the amine substrate occurs during iron/ascorbate-promoted lipid peroxidation also, but H2O2 or free OH. radicals do not appear to be involved. Cumene hydroperoxide-sustained rabbit liver microsomal turnover of the amine generates N-oxy product via O2-dependent and -independent pathways; propagation of lipid peroxidation is presumed to govern the former route. Lipid hydroperoxides, either exogenously added to rabbit liver microsomal suspensions or enzymically formed from arachidonic acid in ram seminal-vesicle microsomal preparations, support N-oxidation of 4-chloroaniline. The significance, in arylamine activation, of lipid peroxidation in certain extrahepatic tissues exhibiting but low mono-oxygenase activity is discussed.

Aniline Compounds↗
PubMed1984

Analysis of proteins, helper dependence, and seroepidemiology of a new human parvovirus.

A new type of defective parvovirus, tentatively designated as adeno-associated virus type 5 (AAV-5), is characterized as far as its proteins, its helper dependence, and its seroepidemiology are concerned. The protein analysis of AAV-5 in polyacrylamide gels demonstrated the presence of three structural polypeptides, corresponding to VP 1, VP 2, and VP 3 of other AAV types. The preparation of monoclonal antibodies against AAV-5 permitted the analysis of viral structural antigen expression by using adenovirus type 12 (Ad 12) or several herpes group viruses as helper viruses, respectively. AAV-5-infected cell cultures coinfected with either Ad 12, Herpes simplex virus (HSV), Cytomegalovirus (CMV), or Varicella Zoster virus (VZV) efficiently synthesize AAV-5 specific antigens. Epstein-Barr virus (EBV) and Herpesvirus saimiri, in contrast, provide only a very weak helper activity for AAV 5 antigen expression. The development of a specific ELISA test permitted screening of human sera for antibodies to AAV-5. Forty-five percent of 926 sera from all age groups and approximately 60% of the adult population reveal antibodies to structural components of this virus. The seroepidemiology differs from that reported for other AAV serotypes. Highest average titers against AAV-5 are observed in the age group between 15 and 20 years. Sera from patients with cervical carcinoma revealed average titers of antibodies well below those of age-matched control groups. Attempts to find higher antibody levels against AAV-5 in specific human diseases failed thus far.

Adolescent↗
PubMed1984

The influence of genetic background on the susceptibility of inbred mice to streptozotocin-induced diabetes.

Multiple low-dose injections of streptozotocin (STZ) induce a delayed but progressively increasing state of hyperglycemia in mice. Different inbred strains of mice show different susceptibility to this treatment. We examined whether genetic factors associated with the H-2 complex influence the susceptibility or resistance, using a selected group of 12 inbred and 5 congenic resistant strains of mice. We found that different congenic strains differed significantly in their susceptibility to STZ-induced diabetes, suggesting that H-2-associated genes do influence the susceptibility. However, at least some inbred strains sharing the same H-2 haplotype also differed in their susceptibility, indicating that genes outside the H-2 complex may also affect the susceptibility. Therefore, there appear to be at least two genes, one within and one or more outside the H-2 complex, that determine the susceptibility to multiple low doses of STZ.

Animals↗