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Biomedical subjects

J Wilson

Publications and source records attributed to J Wilson.

At least 721 records · Page 40Linked to original sources
PubMed1982

Development of secretory protein synthesis in the seminal vesicles and ventral prostate of the male rat.

Functional development of the ventral prostate and seminal vesicles in the male rat has been examined by incubating tissue with [35S] methionine and using specific antisera to detect the synthesis of individual marker proteins. 4 major secretory proteins of the seminal vesicles were chosen along with 4 major prostatic secretory peptides, including 3 which form the prostatic steroid-binding protein. None of the seminal vesicle proteins was synthesised before 25-30 days but all were then induced in parallel to reach adult values by 45-50 days. The 3 peptides of the prostatic steroid-binding protein were synthesised at the earliest age studied (10 days) and were also induced in parallel to maximal levels by 25-30 days. In contrast, the 4th prostatic marker, a glycopeptide, was not synthesised until about 25 days. Treatment of male rats from 3 days of age with testosterone induced precocious development of the seminal vesicles, causing substantial synthesis of marker proteins well before their synthesis would normally have been expected, and also advanced the synthesis of the prostatic 22 K glycopeptide. The results confirm that functional development in the ventral prostate precedes that of the seminal vesicles but that within the ventral prostate there is a considerable difference in the developmental profiles of its secretory proteins.

Animals↗
PubMed1982

A comparison of freezing and thawing methods for the cryopreservation of human semen.

The purpose of this study was twofold. In the first part are compared cryosurvival rates for human semen following two different freezing and three different thawing techniques. In the second part a larger number of samples processed by the best of the six methods described were examined to ensure reproducibility. Eighteen human semen samples with initial motility greater than 60% and density greater than 20 X 10(6)/ml were mixed with a cryopreservative medium at 35 degree C and vacuumed into 0.5 ml straws. Six aliquots were prepared from as many specimens as possible. Three straws were frozen by a programmed method (P) and three by a rapid freezing technique (V). All six straws were stored in liquid nitrogen vapor (-196 degree C) for 1 week. The straws were thawed by 1 of 3 methods: (1) room temperature for 10 minutes and 37 degree C hot plate for 10 minutes, (2) ice water for 10 minutes and 37 degree C hot plate for 10 minutes, (3) 35 degree C water for 12 seconds and 37 degree C hot plate for 10 minutes. Postthaw motility was assessed for each aliquot. The freeze/thaw method P/1 was judged optimal. In the second part of the study a larger number of samples were processed by P/1. The mean +/- standard deviation postthaw motility o 57 semen specimens processed by this method was 61.4 +/- 12.1.

Freezing↗
PubMed1982

Environmental chemodynamic studies with terbufos (Counter) insecticide in soil under laboratory and field conditions.

The effect of temperature, soil moisture, soil type, adsorption, and formulation on terbufos persistence in soil was studied under laboratory conditions. Temperature appeared to be more important than moisture in influencing the dissipation rate of terbufos. More terbufos was recovered from a granular formulation than a technical formulation one month after incubation. Soil adsorption coefficient and calculated first order rate constant were well correlated. Terbufos was relatively immobile on soil thin layers and in field-located soil columns. Laboratory data agreed reasonably well with measurements of persistence in the field. Terbufos degradation kinetics were qualitatively analyzed and the toxicological significance of studying parent terbufos alone was discussed.

Organothiophosphorus Compounds↗
PubMed1982

A randomised trial and a comparative study of the copper 7 200 and the multiload copper 250 intrauterine devices.

A randomized trial of the Copper 7 200 (Cu 7 200) and the Multiload Copper 250 (ML Cu 250) intrauterine devices (IUD) was carried out for 13 months. On life table analysis at 12 months, net cumulative closure rates for use-related terminations (net rates) for the Cu 7 200 were higher than for the ML Cu 250 for accidental pregnancy (NS), expulsions (P less than 0.02) and medical removals for pain and/or bleeding (P less than 0.01). The continuation rate was lower for the Cu 7 200 (P less than 0.001). In a larger comparative study, at 12 months there was a highly significant difference (P less than 0.001) in favour of the ML Cu 250 in accidental pregnancy, expulsions and removals for pain and/or bleeding and continuation of use. Since the ultimate purpose of the use of an IUD is pregnancy protection with a minimum of side effects, the continued use of the Cu 7 200 is not recommended.

Clinical Trials as Topic↗
PubMed1982

Value of computerised tomography in children with non-specific mental subnormally.

Seventy-six children had computerized tomography scans as part of an investigation of mental subnormality; most of them are severely retarded and all those over age 5 years attend special schools. Seventy-two per cent of the children had normal scans. Twenty per cent showed cerebral atrophy and in only 8% was there a specific abnormality (agenesis of corpus callosum, arachnoid cyst, communicating hydrocephalus). None of these findings had any positive prognostic implication. Sedation or general anaesthesia was required for all except one child. Injection pethidine compound was used for children under age 5 years or less than 30 kg in weight, and trimeprazine orally was used for older children. The radiation exposure was high--5.6 rad per scan, which is 100 times greater than that from a posteroanterior and lateral chest x-ray film. For these reasons computerised tomography scans cannot be recommended as a routine part of the investigation of children with non-specific mental subnormality.

Adolescent↗
PubMed1982

Bone demineralisation in patients with Turner's syndrome.

The hypothesis that the demineralisation associated with gonadal dysgenesis is analogous to post-menopausal osteoporosis was investigated. Bone mineral content of the distal forearm was measured in 11 adult patients with Turner's syndrome aged 18 to 57 years. As a group these patients were significantly demineralised (p less than 0.001) when compared with normal subjects. A bimodal distribution of bone mineral was demonstrated, the eight patients below the normal range having a bone mineral content 73% of normal. This may be the usual bone mineral content for a large proportion of Turner's patients. No steady reduction in mineralisation with age was demonstrated. The number of osteoporotic type fractures was obtained from the records of 36 adult patients with Turner's syndrome. From the cumulative total years at risk (770 patient years) from the age of 15 years, it was found that the number of fractures of the distal radius corresponded to the normal premenopausal rather than post-menopausal fracture incidence. The absence of any reduction in bone mineral content with age and no clear evidence of an increase in frequency of fractures both suggest that the demineralisation associated with Turner's syndrome is not analogous to post-menopausal osteoporosis. The regular use of long term oestrogen therapy as a treatment for 'osteoporosis' in these patients is therefore not justified.

Adolescent↗