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Biomedical subjects

J Weill

Publications and source records attributed to J Weill.

At least 73 records · Page 4Linked to original sources
PubMed1979

Hemocyanins in spiders, VII. Immunological comparison of the subunits of Eurypelma californicum hemocyanin.

The isolated subunites of Eurypelma californicum hemocyanin were studied by aid of antibodies raised against whole, dissociated hemocyanin. The proportion of impurities was found to be low in almost all subunits. There was no cross reaction between the individual chains, and the total number of antigenically different subunits was found to be seven, confirming results obtained by different methods. If an artificial mixture prepared from purified subunits is compared to whole, dissociated hemocyanin, an overall very similar pattern is obtained but differences appear which are due to specific interaction.--The dimeric subunit 4D was shown to be a heterodimer (asymmetric dimer) composed of chains b and c4.

Animals↗
PubMed1977

[Structure of scorpion hemocyanin: heterogeneity of its sub-units].

Six dissociation products isolated from the Androctonus australis scorpion haemocyanin are studied through analytical ultracentrifugation and acrylamide-SDS electrophoresis: the results show that one of these fractions exists as a dimer and that the five others are monomers. A genetical study shows that two subunits at least can undergo mutations.

Animals↗
PubMed1976

[Isolation of 6 subunits of scorpion, Androctonus australis garzonii, hemocyanin].

A method for the purification of the 5 sub-units 5 S and of a 7 S sub-unit of the haemocyanin of the Scorpion Androctonus australis garzonii is reported. It includes preparation of crude haemocyanin, dissociation in 1 M urea in the absence of divalent cations, starch gel electrophoresis and A 50 DEAE "Sephadex" separation. All the sub-units are homogeneous with regard to ultracentrifugation and polyacrylamide gel electrophoresis. The yield variers from 7 to 20 per cent according to each fraction.

Animals↗
PubMed1976

[Compared efficiency of mean corpuscular volume (MCV) and serum gamma-glutamyltransferase (gamma-GT) as screening tests for excess-ethanol drinkers (author's transl)].

53 percent of ethanol drinkers had, before detoxication, a gamma-GT higher than the upper limit of the reference interval at the 2.5 percent risk level (36 mU/ml). 44 percent had a mean corpuscular volume (MCV) higher than the limit (99.2 mum3). In alcoholics not previously "weaned" during a rest cure or in a hospital the proportion becomes 67 percent for gamma-GT but remains at 44 percent for MCV. gamma-GT thus seems a better test for the screening of an excessive ethanol intake than MCV, especially when the subject has not been previously weaned.

Alcoholism↗
PubMed1976

[Alpha-1-antitrypsin, orosomucoid, transferrin and alpha fetoprotein in the amniotic fluid, Changes in concentration in normal and pathologic pregnancies].

In this article, which is a preliminary report, the authors determined amniotic fluid levels of alpha-I-antitrypsin, orosomucoid, transferrin and alpha-fetoprotein during complicated pregnancies with gestational ages of 31 to 42 weeks. These levels were compared with those observed during normal pregnancies. The clinical value of the variations of the glycoprotein levels is discussed.

Amniotic Fluid↗
PubMed1975

[Decrease in serum gamma-glutamyltranspeptidase following abstention from alcohol in cirrhotics (author transl)].

Gamma-Glutamyltranspeptidase activity of patients having an ascitic cirrhosis due to ethanol consumption is high (139 mU/ml) when the patient is still drinking at the time of the assay; it is lower when the patient had stopped drinking at least two months before the assay (49 mU/ml). On the other hand, in 10 patients out of the 11 who submitted to a second assay gamma-glutamyltranspeptidase decreases as soon as the patient abstains from alcohol. In 4 abstinent patients re-examined one year after the first measurement, the gamma-glutamyltranspeptidase activity had decreased to the reference values of Szasz. The half time of the return to normal has been estimated by extrapolation from the ethylic model at between 11 and 54 days. We conclude that the hyper gamma-glutamyltranspeptidase in cirrhotics is due to the ethanol impregnation and that repeated assays of the enzyme show whether the patient abstains from alcohol or not.

Alcohol Drinking↗
PubMed1975

[Use of the measurement of gamma-glutamyltranspeptidase activity in serum to determine the success of cures for alcohol detoxification (author's transl)].

44 patients were studied during a 2-year period following a cure for alcohol detoxification. 29 patients (group A) did not start drinking again while 15 relapsed less than one year after their cure (group R). The average gamma GT activity (m) in mU per ml serum was, in group A, 148 (standard deviation, S.D. 184) at the beginning of the cure, 21 (S.D. 14) after one year and 19 (S.D. 13) after 2 years. During the same period there was no significant decrease in group R (cure: m 140, S.D. 161: 1 year: m 166, S.D. 164; 2 years: m 162, S.D. 163). On the other hand, all the subjects of group A who had a high gamma GT activity (greater than 29 mU/ml) at the start of the cure showed a decrease after one year.

Alcohol Drinking↗
PubMed1975

[Serum gamma-glutamyl-transpeptidase and alcoholism. Diagnosis and control of withdrawal].

The gammaGT of a generally healthy population is abnormally high due to the presence of ethanol in the diet. We confirm Rosalki's finding that a high proportion of alchoholics show an increased gammaGT activity. After alcohol deprivation, the gammaGT of alcoholics decreases in the first few days according to an exponential law with a half-time of return to normal of 5-17 days. Among past alcoholics, those who stopped drinking for a year have a lower GT activity (mean: 21 mU/ml) than a generally healthy population and in the range of Szasz's reference values. Alcoholics who did not stop drinking have a higher gammaGT activity (mean: 172). Ascitic cirrhotics who drink at least 1 liter of wine per day have also a high activity (mean: 139) while those who stopped drinking for at least 2 months have a much lower activity (mean: 49) and those who stopped for a year a normal activity. In 10 out of 11 alcoholic cirrhotics who stopped drinking, the gammaGT decreased (half-time: 11-54 days). These results show that the determination of gammaGT activity is a good test for the detection of ethanol impregnation.

Adult↗
PubMed1975

[Automation of extraction in the determination of blood triglyceride].

A complete automation of the determination of triglycerides is described which replaces the manual extraction and the final determination with an autoanalyzer chain, based on the method of Royer et al. For the automation of triglyceride extraction, we have modified the sampler, by incorporating a magnetic stirrer under the sample table. The sample table has two rows of holes. The sera, which are taken from the inner row and diluted with sulphuric acid, are reacted quantitatively with the extraction reagent in the beakers of the outer row, which contain a magnetic follower. After stirring, the upper phase is quantitatively removed and the determination is started. The problem of phasing the individual steps of the analysis is thus solved.

Autoanalysis↗