Search PubMed⌕ Search

Biomedical subjects

J Vidal

Publications and source records attributed to J Vidal.

At least 271 records · Page 15Linked to original sources
PubMed1982

Glutamine Synthetase in Spinach Leaves : IMMUNOLOGICAL STUDIES AND IMMUNOCYTOCHEMICAL LOCALIZATION.

By polyacrylamide gel electrophoresis, DEAE Sephacel, and hydroxyapatite chromatography, one form of glutamine synthetase has been identified in spinach (Spinacia oleracea L. cv. Monstrueux de Viroflay) leaves. It is localized only inside the chloroplast. The enzyme was purified to homogeneity and specific antibodies against the protein were raised by immunization of rabbits. The intracellular localization of glutamine synthetase in spinach leaves was studied by indirect immunofluorescence microscopy on thin-sectioned spinach leaves. It has been demonstrated that the enzyme is specifically associated with the chloroplasts of parenchymatous cells.

Journal Article↗
PubMed1982

Glutamate synthase isoforms in rice: immunological studies of enzymes in green leaf, etiolated leaf, and root tissues.

Rabbit antiserum was raised against ferredoxin-dependent glutamate synthase (EC 1.4.7.1) purified from green leaves of Oryza sativa L. cv Delta. Ferredoxin-dependent glutamate synthase, detected in green leaf, etiolated leaf, and root tissues cross-reacted completely with the antiferredoxin glutamate synthase immunoglobulin G. In contrast, the immunoglobulin G did not cross-react with NADH-dependent (EC 1.4.1.14) and NADPH-dependent (EC 1.4.1.13) glutamate synthases found in nonphotosynthetic etiolated leaf and root tissues. In addition, ferredoxin-dependent glutamate synthase was separated and distinguished by its affinity to ferredoxin from NAD(P)H-dependent glutamate synthase on ferredoxin-Sepharose affinity chromatography. Based on the immunological studies, it is suggested that ferredoxin-dependent glutamate synthases in green leaf and etiolated leaf tissues are closely related proteins; in contrast, ferredoxin-dependent glutamate synthase in root tissue is a distinct protein from the leaf enzymes.

Journal Article↗
PubMed1982

[Tibio-fibular graft as a treatment of non-union of the tibia (author's transl)].

The authors have reviewed 47 cases of septic non-union of the tibia treated by tibio-fibular graft, Bone union was obtained in 31 cases, 2 limbs were amputated and 13 tibiae were united after another procedure. In 17 cases the grafts were performed in the presence of a discharging sinus. These cases did as well as any others. In cases of bone loss after diaphyseal resection, tibio-fibular grafting appeared to be a better procedure than Papineau's technique. Several cases of failure of this technique were cured successfully by tibio-fibular grafting when the bone loss was extensive, tibio-fibular grafting had to be completed by a supplementary tibial graft. The authors consider that grafting procedure should be done early in cases of extensive bone loss or in cases of severe compound comminuted fracture.

Adult↗
PubMed1982

[Scarification: its role in the treatment of chronic fistulated pyogenic osteoarticular infection].

The "scarification" technique is a reliable method of healing chronic bone infection by promoting revascularisation. A wide excision of scarred or infected soft tissue is first carried out. The bone is then carefully "scarified", or petaled, with minimal removal of bone substance. A small window is made to allow drainage and curettage of the intramedullary space. Revascularisation of diseased bone is stimulated by this technique, thus encouraging the growth of granulation tissue with consequent healing. The authors claim a success rate of 93% in 86 patients.

Adolescent↗
PubMed1981

[A study of scoliotic curve. The importance of extension and vertebral rotation (author's transl)].

The authors consider that scoliotic curves should be studied in the spatial planes, not only in the frontal plane. Lateral views show that the displaced segments of the spine are always an extension even when there is a kypho-scoliosis. This element must be considered when the deformity is about to be reduced or treated by bracing. In the axial plane, the rotational displacement must be measured. In a dorsal curve, there is specific rotation of the upper vertebrae. The measurement of this rotation may make it possible to predict the extent of the curve at the end of growth in cases of spontaneous evolution.

Biophysical Phenomena↗
PubMed1981

Results of operations upon abdominal aortic aneurysms at a community hospital.

Review of a retrospective series of 140 consecutive abdominal aortic aneurysms in a community hospital has disclosed a low operative mortality and a low morbidity rate. Seventy patients were more than 70 years of age. Of 82 patients operated upon before rupture of the aneurysm, three died and 11 had complications. Of 38 patients operated upon after rupture of the aneurysm, 22 died and eight had complications. The age of 70 years or older and shock were identified as significant risk factors adversely affecting the outcome of operations done as an emergency upon patients with ruptured aneurysms. Renal failure occurred only in patients with ruptured aneurysms; when renal failure did occur, it was usually fatal. Myocardial infarction seldom occurred after elective operations because of aggressive, thorough cardiac evaluation, including selective coronary arteriography. Myocardial infarctions contributed to the deaths of six patients in whom the aneurysms ruptured prior to operation.

Aged↗
PubMed1979

Humero-spinal dysostosis.

A 2 year old boy with humero-spinal dysostosis is described. This is the third case of this disease reported in the literature. Humero-spinal dysostosis is characterised radiologically by distal humeral bifurcation, elbow subluxation and coronal cleft vertebrae. Congenital, progressive heart disease, possibly with fatal outcome, is probably part of the syndrome.

Bone Diseases↗
PubMed1979

[Radiological manifestations of Meckel's diverticulum (author's transl)].

Multiple clinical and radiological manifestations of Meckel's diverticulum are commented, presenting four cases with acute abdominal manifestations and one with chronic symptomatology, which were selected from a series of nineteen cases that were surgically verified. The authors realize a critique of the radiological techniques in the literature of the suspected cases of said entity.

Child↗
PubMed1978

[Experience and application of diagnostic pneumoperitoneum in the anomalies in the umbilical region (author's transl)].

By means of 30 pneumoperitoneograms performed "postmortem" and five performed for diagnosis, the exploratory technique, radiologic anatomy of umbilical region, and the usefulness of the process in the diagnosis in children are discussed. Pneumoperitoneum may be indicated in diagnosis of bleeding Meckel's diverticulum, in exclusion or confirmation of remnants of the omphalomesenteric duct in cronically moist lesions of the umbilicus resistent to symptomatic treatment, in cases of non-communicating urachal cysts which can not be diagnosed by cystogram, and in the differential diagnosis of abdominal tumors related to the umbilical region.

Child, Preschool↗
PubMed1977

Indications for pneumoperitoneum in the diagnosis of congenital anomalies in the umbilical region.

Thirty pneumoperitoneograms were performed postmortem and 5 were performed for diagnosis: the technique itself, the radiological anatomy of the umbilical region, and the usefulness of the procedure in diagnosis are discussed. Pneumoperitoneum may be indicated in the investigation of a bleeding Meckel's diverticulum, in the exclusion or confirmation of remnants of the omphalomesenteric duct, in chronically moist lesions of the umbilicus resistant to symptomatic treatment, in suspected cases of non-communicating urachal cysts which cannot be diagnosed by cystogram, and in the differential diagnosis of abdominal tumours related to the umbilical region.

Abdominal Neoplasms↗