Biomedical subjects

J V Schneider

Publications and source records attributed to J V Schneider.

3 recordsLinked to original sources

PubMed1982

Selenium in livestock production: a review.

A review of the literature concerning the role of selenium in livestock is presented. Selenium was originally known for its toxicity but in 1957 it was discovered that supplementation of this element prevented liver necrosis in rats. The occurrence of selenium in soils and uptake by plants is discussed, as well as the biochemistry, metabolism, interactions with other elements and nutrients, known functions, requirements of livestock and its toxicity.

Animal Nutritional Physiological Phenomena↗

PubMedDate not supplied

Some quantitative aspects of the human monocyte erythrophagocytosis and rosette assays.

The sensitivity of peripheral blood mononuclear phagocytes (PBMPs) in the red cell (RBC) adherence and erythrophagocytosis assays were investigated using anti-D of subclasses IgG1 and IgG3. Particular emphasis was placed on identifying variability between PBMP preparations from different healthy donors. About 500 molecules of IgG1 type anti-D per RBC and about 100 molecules of IgG3 antibody per RBC were the lowest levels of sensitization which elicited a weak, positive test. Considerable variation between different preparations was seen, and it appeared that the capability of a PBMP preparation to react with weakly sensitized RBCs may not be closely related to its strength of reaction with strongly sensitized cells. PBMPs which were recovered after storage in liquid nitrogen were more reactive in both assays than freshly prepared PBMPs from the same donor. This study demonstrated the importance of weakly sensitized RBCs as part of the control system and indicated a need for caution when establishing a negative test result when the clinical significance of RBC antibody is being investigated.

Blood Preservation↗

PubMedDate not supplied

A solid-phase antiglobulin test.

A solid-phase test (SPT), a machine-readable adaptation of the indirect anti-human globulin test (IAHG), is described. The SPT level of sensitivity was comparable to that of the manual IAHG test for the detection of IgG antibodies and was compared with manual pretransfusion compatibility tests. The average difference from the mean of duplicate SPT results was 5.94 percent of the optical density value. The data also demonstrated a linear and overlapping relationship between the scores of manual IAHG tests and SPT optical density values. The accuracy of the SPT declined slightly as the concentration of red cell samples increased; the optimum concentration of immunoglobulin used in the preparation of the solid phase was about 275 mg per l. The 97.5th percentile of negative tests occurred at the same optical density (0.13) when serums from either healthy donors or hospitalized patients were tested. Compatibility tests completed on 1304 blood samples from donors and 437 serum samples from hospitalized patients showed that 1.38 percent of results were SPT-positive and manual IAHG-negative, and 5.37 percent were SPT-negative and manual IAHG-positive. The lowest possible score in the manual test was allocated to 97.14 percent of the latter group, indicating good correlation between the two techniques.

Coombs Test↗