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Biomedical subjects

J Ursing

Publications and source records attributed to J Ursing.

At least 55 records · Page 3Linked to original sources
PubMed1988

New naphthalene-degrading marine Pseudomonas strains.

Over 100 strains that utilized naphthalene as the only carbon and energy source were isolated from samples of marine sediments taken from a heavily polluted area. The isolates were characterized taxonomically and physiologically. Most of these strains belonged to the genus Pseudomonas, and seven of them did not fit any previous taxonomic description. They differed from type strains in a few biochemical characteristics and in the utilization of aromatic compounds. None had catechol 1,2-dioxygenase activity, and catechol 2,3-dioxygenase was responsible for the aromatic ring cleavage. DNA hybridization demonstrated a close relationship between two isolates and the Pseudomonas stutzeri type strain, and between five isolates and the Pseudomonas testosteroni type strain. On the basis of nutritional and enzymatic characteristics, it was assumed that the seven isolates represent new biovars belonging to the species P. testosteroni and P. stutzeri that are able to degrade aromatic hydrocarbons.

Biodegradation, Environmental↗
PubMed1988

Fosfomycin versus ampicillin in the treatment of acute pyelonephritis.

In a controlled open study of 38 patients with acute pyelonephritis, treatment with fosfomycin 8 g b.i.d. was compared to treatment with ampicillin 2 g t.i.d., both for one week. The most common pathogen in both groups was Escherichia coli, susceptible to fosfomycin. However, 17% were resistant to ampicillin. The success rate was 44% in the fosfomycin treated group and 28% in the ampicillin group. The difference was not significant (p greater than 0.20). Peak concentrations of fosfomycin in serum were 395 mg/l and in tissue fluid 85 mg/l. Urine concentrations ranged from 6990 to 24,320 mg/l.

Acute Disease↗
PubMed1987

Genetic heterogeneity of Flavobacterium meningosepticum demonstrated by DNA-DNA hybridization.

DNA-DNA reassociation studies on 52 strains of Flavobacterium meningosepticum showed two main hybridization groups about 40-55% interrelated and comprising 4 and 48 strains respectively. The larger group could be further divided into four subgroups if differences in thermal stability of the reassociated duplexes were taken into consideration. Of a total of 20 strains isolated from cerebrospinal fluid, 18 were found in one subgroup of 28 strains, which also contained seven blood isolates. The results indicate that genetically defined subgroups within the species might differ with regard to pathogenic significance.

DNA, Bacterial↗
PubMed1987

Phenotypic characterization of Flavobacterium meningosepticum strains identified by DNA-DNA hybridization.

Fifty-two strains found to belong to Flavobacterium meningosepticum on the basis of DNA-DNA hybridization analyses were characterized and found to be phenotypically homogeneous. All strains were oxidase, catalase, indole, gelatinase and beta-galactosidase positive, and produced acid from glucose, mannose, fructose, maltose and mannitol; nitrate was not reduced. F. meningosepticum could be differentiated from Flavobacterium group IIb by its ability to produce beta-galactosidase, and by the latter taxon's ability to produce a bright yellow pigment in contrast to the weak or non-existing pigmentation of F. meningosepticum. Phenotypic characteristics that could differentiate between the two main DNA relatedness groups of F. meningosepticum were not discovered, wherefore a subdivision of the present species into two species cannot be recommended. Strains from the DNA relatedness groups comprising 19 of 20 CSF isolates were found to be able to grow at 40 degrees C and to produce a weak yellow pigment; in contrast, strains from the three other DNA relatedness groups were unable to grow at 40 degrees C and produced no pigment.

DNA, Bacterial↗
PubMed1986

Clinical strains of Enterobacter agglomerans (synonyms: Erwinia herbicola, Erwinia milletiae) identified by DNA-DNA-hybridization.

Using DNA-DNA-hybridization it could be shown that 52 of 86 clinical isolates of Enterobacter agglomerans were closely related to each other, to the type strain of the species and also to the type strains of Erwinia herbicola and Erwinia milletiae. Most of the strains investigated were of the biogroups 1 and G1 of Ewing & Fife. All strains of the genetically defined group belonged to these two biogroups; none of these isolates fermented dulcitol, and with few exceptions they were also cellobiose, lactose and sorbitol negative.

DNA, Bacterial↗
PubMed1985

Antibiotic treatment during surgery for diffuse peritonitis: a prospective randomized study comparing the effects of cefuroxime and of a cefuroxime and metronidazole combination.

In a prospective randomized open study of patients operated upon for diffuse peritonitis, the effects of two different antibiotic regimens were evaluated. Cefuroxime given as a single drug (Group I; n = 59) was compared with a combination of cefuroxime and metronidazole (Group II; n = 63). Bacteriological cultures, both aerobic and anaerobic, were obtained peroperatively and in the event of any complication. The antibiotic sensitivities of isolated bacteria, and the serum and tissue concentrations of cefuroxime were determined. Postoperative infectious complications occurred in 22 per cent of Group I patients (cefuroxime), and in 17.5 per cent of Group II (cefuroxime plus metronidazole). The mortality rates were 5 per cent for Group I and 8 per cent for Group II. Tissue concentrations of cefuroxime were well above the MIC (minimal inhibiting concentration) values for most of the bacteria isolated. From a few patients in Group I, however, cultures were obtained with isolates sensitive to metronidazole but resistant to cefuroxime. Our findings suggest that, in the antibiotic treatment of patients operated for diffuse peritonitis, an agent which is primarily effective against aerobic bacteria (but not entirely without effect on anaerobes) is as effective as combination therapy covering both aerobic and anaerobic bacteria.

Adolescent↗
PubMed1985

Genetic relationship between "Campylobacter fecalis" and Campylobacter sputorum subsp. bubulus.

Nine strains of "Campylobacter fecalis" were compared with two references strains of C. sputorum subsp. bubulus considering DNA base composition and DNA-DNA-hybridization. The results showed that the two taxa could not be separated by these methods, their mol%(G+C) being 31-32 and DNA relatedness 79% or more. It is suggested that the catalase-positive "Campylobacter fecalis" should be regarded as a subspecies of the catalase-negative C. sputorum.

Animals↗
PubMed1984

Immunological aspects of humidifier fever.

An epidemiological investigation was made of eight employees working in a defined section of an office building who complained of febrile reactions accompanied by respiratory tract symptoms and fatigue. Culture of the water from the humidifier for their section yielded strains of Pseudomonas acidovorans and an unidentified Pseudomonas species. In vitro studies showed that these strains activated the alternative pathway of the complement system in Mg-EGTA supplemented serum, as evidenced by properdin depletion and conversion of C3-proactivator (Factor B) and C3. Whereas the employees with symptoms had significantly reduced properdin values during the symptomatic phase, a slight C3 conversion and antibodies to Pseudomonas spp., staff working in another part of the building with a different ventilation system did not. It was concluded that staff with humidifier fever symptoms had been exposed to Pseudomonas spp. through the faulty ventilation system, and their symptoms were most likely a result of complement activation.

Adult↗
PubMed1984

Genetic and phenotypic characteristics of a new group of Campylobacter isolated from pigs and cattle.

Five intestinal strains of Campylobacter isolated from pigs and cattle are described. The strains grew in anaerobic as well as in micro-aerobic environment; they were catalase positive and resistant to nalidixic acid but sensitive to cephalothin and metronidazole. They had a mean G+C content of 35.4 mol %. DNA-DNA-hybridizations showed that the group was homogeneous: about 50% related to C. fetus ss fetus and less than 25% related to other Campylobacter species.

Animals↗
PubMed1981

Single dose doxycycline prophylaxis and peroperative bacteriological culture in elective colorectal surgery.

A prospective study was performed on 196 consecutive patients undergoing elective colonic surgery to evaluate the prophylactic effect of a single dose of doxycycline. The patients were randomized into four groups: group I 200 mg i.v. preoperatively, group III 600 mg i.v. preoperatively, group III 600 mg i.v. postoperatively, and group IV 200 mg i.v. preoperatively and 200 mg i.v. daily 3 days postoperatively. The rate of septic complications for the different groups were: I 13 per cent, II 7 per cent, III 20 per cent, and IV 19 per cent. There was no statistically significant difference in occurrence of septic complications between the groups. The degree of bacterial contamination during operation was estimated by culture from wound irrigation fluid and from cotton swabs. Bacteria were recovered from the irrigation fluid in 97 per cent, while culture from cotton swabs proved to be much less sensitive. A high number of bacteria in the irrigation fluid was significantly correlated to a high complication rate. It is suggested that direct plating from irrigation fluid can be used for defining a high risk group of patients in colonic surgery.

Aged↗
PubMed1981

Incidence of Legionella pneumophila in acute lower respiratory tract infections.

The etiological role of Legionella pneumophila and other infectious agents in acute lower respiratory tract infections in 112 patients attending an infectious disease clinic was estimated by a serological study of paired sera and bacteriological culture of nasopharynx swabs and culture and immunoelectroosmophoresis of expectorates. Only 2 of the patients had a 4-fold rise in antibody titre to L. pneumophila, suggesting a similar incidence of legionnaires' disease as reported from USA.

Adolescent↗
PubMed1979

Bacterial infection in relation to pain and irregular bleeding in women using IUD.

Pain and metrorrhagia in women using IUDs are often the reason for removing the IUD. In 54 women where the IUD was removed, uterine fluid for bacteriologic cultures was taken from the removed IUD. There was a correlation between positive cultures and symptoms of pain and metrorrhagia. Spotty bleeding caused by microtraumata and increased fibrinolytic activity of the IUD might thus consitute a substrate for bacterial invasion resulting in endometritis and in turn increased bleeding and pain. In 18 male consorts of females with positive cultures, asymptomatic bacteriospermia was found. Treatment offered alleviated symptoms. Removal of the IUD need thus not be necessary.

Bacterial Infections↗
PubMed1977

Characterization of Enterobacter agglomerans (Erwinia spp.) from clinical specimens.

The biochemical reactions of 18 anaerogenic and 45 aerogenic isolates of Enterobacter agglomerans are described and used for subdivision in the biogroups suggested by Ewing & Fife. The presence of special cultural characteristics (symplasmata and biconvex bodies) was also recorded and was found to be unrelated to formation of gas from glucose. Previous authors have reported such structures in anaerogenic cultures only. The amount of gas produced varied to a great extent between strains and also proved to be dependent on incubation temperature. The results do not support the division of E. agglomerans into one anaerogenic and one aerogenic major subgroup. The value of the gelatin liquefaction test for characterization of the species is emphasized.

Bacteriological Techniques↗