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Biomedical subjects

J Takeuchi

Publications and source records attributed to J Takeuchi.

At least 289 records · Page 16Linked to original sources
PubMed1982

[Transportation of cefotiam from serum to cerebrospinal fluid (author's transl)].

Cefotiam (1 g) was administered by one-shot intravenous injection to the patients proceeding a brain surgery. The cerebrospinal fluid (CSF) and serum were taken as a specimen, and the concentration CTM was assayed by agar-well method using Proteus mirabilis ATCC 21100. The most high concentration of CTM was 2,273 micrograms/ml in serum, and 2.3 micrograms/ml in CSF, respectively. The concentration of CTM in serum, CSF, and CSF/serum ration were determined at the indicated time. It appears likely that CTM can pass into CSF more easily than other cephalosporins.

Adult↗
PubMed1982

[Detection and clinical significance of immune complexes in the sera of brain tumor patients].

Levels of circulating immune complexes (IC) in the sera of 87 patients with brain tumor were measured by Raji cell radioimmunoassay and Clq binding assay. The control samples were obtained from 40 healthy blood donors and 40 cerebrovascular patients. The patients with glial tumor had a high incidence of elevated level of IC than patients with non-glial benign tumor, elevated level of IC than patients with non-glial benign tumor, cerebrovascular disease and normal controls. There was a significant association between these Clq binding activity and Raji cell binding activity in the sera of glial tumor patients. As regards the sensitivity, high IC levels are more frequent among Raji cell assay than Clq binding assay. The level of IC was found to be higher in patients with recurrent glial tumor than in those with primary tumors. However, the level of IC in the sera from patients with glial tumor did not always decrease during remission after successful treatment. Our data indicate that the measurement of IC may prove useful in the management of patients with brain tumor, in particular, to detect recurrence or progression of tumor growth.

Antigen-Antibody Complex↗
PubMed1982

Activation of platelets in patients with chronic proliferative glomerulonephritis and the nephrotic syndrome.

Platelet count, volume and aggregation and plasma levels of beta-thromboglobulin (beta-TG) and platelet factor 4 (PF-4) were measured in 54 patients with chronic glomerulonephritis (CGN). Platelet count and platelet aggregation induced by ADP, adrenaline and collagen were significantly higher in the patients than in normal subjects, and platelet aggregation was markedly increased in the cases with progressive glomerular lesions. Plasma levels of beta-TG and PF-4 were significantly higher in the patients than in the normal subjects. There was a significant inverse correlation between plasma beta-TG and creatinine clearance. Nephrotic patients showed significantly smaller platelet volume and markedly elevated plasma beta-TG levels when compared to the controls. Plasma beta-TG decreased remarkably in 3 out of 4 patients with markedly increased beta-TG levels when they were given antiplatelet drugs. The results suggest that platelet aggregation and the release reaction were increased in patients with CGN. Activated platelets may be an important factor in the genesis of the thrombotic tendency in the nephrotic syndrome.

Adolescent↗
PubMed1982

The role of heparin in the changes of lipid patterns during a single hemodialysis.

The effects of heparin administered during dialysis on serum lipids and lipoproteins were evaluated by comparing dialysis with heparin and dialysis without heparin. Gabexate mesilate (GM), a synthetic proteinase inhibitor, was used as an anticoagulant during dialysis without heparin. In dialysis with heparin, significant increases were observed in free fatty acids (FFA), high-density lipoprotein cholesterol (HDL-C), and plasma lipolytic activity (PLA). Triglyceride (TG) was decreased reciprocally and the lipoprotein electrophoretic pattern was markedly modified. In contrast, in dialysis without heparin, no significant changes were observed in all parameters of lipid metabolism. These data suggest that dialysis induced changes in lipids are mainly caused by heparin and that acetate and glucose in dialyzate make an almost negligible contribution to abnormal lipid patterns in patients on maintenance hemodialysis.

Electrophoresis, Agar Gel↗
PubMed1982

[Changes of natural killer activity following local 60Co irradiation in intracranial tumor-bearing mice (author's transl)].

Changes of natural killer activity (NK activity) by local 60Co irradiation in intracranial tumor-bearing mice were studied by the method of 51Cr release assay. Local irradiation was administered 10 days after intracranial transplantation of 203-Glioma which had been originally induced by 20-methylcholanthrene in C 57 BL mice. Irradiation suppressed the growth of tumor and prolonged the mean survival time. The 50% survival time of untreated mice was about 2.5 weeks but that of mice treated by a single dose of 1000 rad and 1500 rad of irradiation was about 4.5 weeks and 6.5 weeks respectively. NK activity of spleen cells in these mice was serially examined. NK activity was gradually increased in mice treated by local irradiation, while it was gradually decreased in mice without treatment. On the other hand, NK activity remained unchanged in non-tumor-bearing control mice. Mice treated with 1000 rad and 1500 rad of irradiation showed 44.0% and 47.6% of % specific 51Cr release respectively 11 days after irradiation while normal mice showed 18.0%. The increased NK activity after local irradiation suggested that local irradiation might have enhanced the immunological defence mechanism against the tumor in the tumor-bearing hosts. Some characteristics of effector cells in this assay system were examined. The cytotoxicity of spleen cells was removed by the treatment of anti-BAT serum and complement but was not removed by the treatment of anti-Thy-1. 2 serum and complement. Since NK activity reflects the immunological resistance to tumors to some extent, it is felt important to clarify the significance of changes of NK activity in patients with brain tumors in relation to various treatments including surgery, radiotherapy, chemotherapy and immunotherapy in the next step.

Animals↗
PubMed1982

[Intracerebral picibanil injection in the rat neurogenic tumor--its histopathological changes].

Penicillin-free-Picibanil was injected into the brain of Wistar/Fibiger strain rat to investigate the histological changes. Its effect on the growth of rat schwannoma inoculated in the brain was also examined histologically. Picibanil caused the most prominent but focal lymphoreticular cell reaction around the injected site in the brain 24 to 48 hours after injection. The cells infiltrating from the blood vessels were neutrophils, monocytes, lymphocyte-like cells, histiocyte-like cells and unidentified cells. This lympho-reticular cell reaction gradually subsided in the next 4 to 5 days. Neutrophils and monocytes (macrophages)stayed till the later phase. Two weeks later, the lesion was minimal with only mild glial changes. Picibanil was however, considered to exert at the site of injection neurotoxic and glia-toxic activity at a concentration of 0.1 KE/10 microliters. The rat schwannoma (T1) cells, when inoculated without any treatment, showed vivid growth in the brain and no lympho-reticular cell reaction was observed. When the T1 tumor cells, suspended in the Picibanil solution, were inoculated into the brain, no lymph-reticular cell reaction was observed 7 and 14 days after inoculation. However, when the rat was pretreated with Picibanil into the brain 7 days before inoculation, only a mild lymphoreticular cell reaction was observed around the T1 cells till 7 days after inoculation. Accordingly, Picibanil was considered to induce nonspecific immunological in the rat brain even around the glioma.

Animals↗
PubMed1982

Hypouricemia due to an increment in renal tubular urate secretion.

Two patients had hypouricemia due to increased uric acid clearance. Their daily urinary uric acid excretions were normal. In both patients, pyrazinamide decreased urinary uric acid clearance to almost while probenecid increased it markedly. No other renal tubular or metabolic abnormalities were found. The serum electrolytes and the urinary electrolytes excretions showed no abnormalities. It appears that increased secretion of uric acid in the renal tubule is most probably responsible for hypouricemia in these patients. This report adds to the list of hypouricemic conditions due to a new isolated renal tubular abnormality.

Adult↗
PubMed1982

[Contrast echocardiographic evaluation of right ventricular filling profiles in patients with right ventricular volume or pressure overload].

UNLABELLED: Contrast echocardiographic examinations were performed to evaluate hemodynamic filling profiles in patients with right ventricular overload as well as in normal adults. The subjects were 15 cases of atrial septal defect (ASD) as a representative of right ventricular volume overload, 13 cases of mitral stenosis (MS) as a condition with right ventricular pressure overload and 15 cases of normal adults. M-mode contrast echocardiograms were recorded through the ultrasonic beam direction determined by two-dimensional echocardiograms as shown in Fig. 1. RESULTS: 1) In diastole, continuous rapid linear flow through the tricuspid valve orifice was observed in 11 patients with ASD, while biphasic rapid linear flow was noted in normal adults (control) and in patient with MS. 2) Maximum flow velocity (MFV) in early diastole and flow velocity in atrial contraction phase (AFV) were larger in cases with ASD than that of the control subjects. However, there was no difference in rapid filling time (RFT) between patients with ASD and MS. 3) The characteristic findings of tricuspid valve echoes in cases of ASD included increased valve opening velocity (TOV), decreased diastolic descent rate (TDDR), and high F point. These findings were correlated well with increased MFV and prolonged RFT. 4) MFV was decreased secondarily to the increment of pulmonary artery pressure. 5) Turbulent flow in the right-sided chamber was observed in patients with pulmonary hypertension. These results suggest that linear contrast echoes in the M-mode echocardiograms may be useful for the evaluation of flow velocity and flow profiles across the tricuspid valve orifice in patients with right ventricular volume or pressure overload.

Adult↗
PubMed1981

A high level of glycosaminoglycan-synthesis of squamous cell carcinoma of the parotid gland.

Glycosaminoglycan content of a squamous cell carcinoma derived from the parotid gland was analyzed. The tumor tissue contained a large amount of glycosaminoglycans, and the amount was strikingly larger than found in other kinds of tumor tissues (pleomorphic adenoma, scirrhous carcinoma, myxoma, etc.) analyzed previously. A culture cell line established from this tumor showed a morphologic characteristic of differentiated squamous cell carcinoma, in vitro, forming many tonofilaments in the cytoplasma and numerous desmosomes in the intercellular connection. The carcinoma cell synthesized and secreted a large amount of glycosaminoglycans, consisting mainly of hyaluronic acid. The amount of 3H-labelled hyaluronic acid secreted by this carcinoma cell was about 20-fold larger than that by HeLa cell or KB cell. Conceivably, a high level of hyaluronic acid synthesis is one of the biologic characteristics of squamous cell carcinoma derived from the duct epithelium of the salivary gland.

Carcinoma, Squamous Cell↗
PubMed1981

Hepatic glomerulonephritis. Characteristics of hepatic IgA glomerulonephritis as the major part.

Glomerular lesions associated with hepatic disease were evaluated. Among 752 consecutive patients with hepatitis and cirrhosis, nephritic urinary changes appeared in 1.0% of chronic hepatitis and 9.2% of cirrhotics, but none in patients with acute or subacute hepatitis. Kidney tissue was obtained from 141 cases, of which 59 underwent immunofluorescent studies. Except for a few with possibly coincidental glomerulonephritis, the main glomerular pathology was mesangial depositive or proliferative lesions with frequent circumferential mesangial interposition. The highest incidence (up to 69.2%) occurred in liver cirrhosis. The glomerular immunohistology was not necessarily homogeneous. In acute or subacute hepatitis, IgG or IgM, if present, was dominant. The more chronic the course the liver disease followed, the more frequently significant IgA deposition emerged, occurring in 60.5% of cirrhotics. The IgA positive cases often disclosed paramesangial dense deposits, which is one of the characteristics of primary IgA nephritis. Hepatic IgA nephritis exhibited a lower nephritogenicity and a proneness to show mesangial interposition when compared with primary or purpuric IgA nephritis. The possible origin of glomerular IgA associated with liver disease is discussed.

Chronic Disease↗
PubMed1981

Hepatic glomerulonephritis. Role of hepatitis B surface antigen (HBsAg).

A possible role of HBsAg in hepatic glomerulonephritis was evaluated in kidney specimens from 104 patients with various diseases. Huang's method using formalin-fixed and paraffin-embedded sections was applied to the kidney from 65 cases. Only 4 (3.8%) with liver cirrhosis had glomerular HBsAg deposition; 3 on frozen and one on paraffin sections. In one case of crescentic glomerulonephritis, HBsAg appeared to have had a pathogenetic role with glomerular immunofluorescence dominant for IgM, less intense IgG and negative IgA. The glomerular HBsAg in the remaining 3 patients with hepatic IgA glomerulonephritis was likely to be concomitant or superimposed. One of them had a nephrotic syndrome for which corticosteroid treatment was given, resulting in a near-complete remission and disappearance of HBsAg in the glomeruli, but the glomerular immunohistology was unaffected by the treatment. The present findings suggest that HBsAg has only a minor role.

Chronic Disease↗