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J Sugar

Publications and source records attributed to J Sugar.

At least 109 records · Page 6Linked to original sources
PubMed1988

Histochemical studies of keratoconus.

We quantitated the amount of collagen in corneas obtained from patients with keratoconus. Following a previously described procedure, we deparaffinized tissue sections and stained them with the collagen-specific dye Sirius red. Twenty-two (group I) of 31 keratoconus corneas examined were normal, whereas the remaining 9 (group II) showed reduced collagen staining per mm2 of surface area compared with the controls. These results agreed with our previous data demonstrating heterogeneity in keratoconus in terms of collagen content analyzed biochemically. In addition, we adapted another histochemical method, using safranin O, to measure the polyanion (mainly glycosaminoglycan) content in paraffin sections of the same specimens. All except two keratoconus corneas showed markedly higher polyanion staining per mm2 of surface area than the controls. This suggests that keratoconus corneas may contain an increased amount of glycosaminoglycans and other polyanions. Such an abnormality may be related to some of the pathologic changes seen in these corneas.

Azo Compounds↗
PubMed1987

An ultrastructural and histochemical study of long-term wound healing after radial keratotomy.

Using correlative microscopy and plant lectins conjugated to fluorescent dyes, we studied two human corneal specimens obtained 66 and 70 months after radial keratotomy. In one case a second radial keratotomy had been performed 11 months before we obtained the corneal specimen, which allowed us to compare wounds 11 and 66 months old in the same specimen. The 11-month-old incisions demonstrated morphologic features consistent with incomplete wound healing, whereas the incisions examined 66 and 70 months postoperatively demonstrated complete wound healing. A different lectin binding pattern of wheat germ agglutinin and concanavalin-A was observed in the 11-month-old wounds, compared with the 66-month-old wounds. Published and unpublished morphologic studies of 17 human keratotomy specimens have demonstrated incomplete wound healing up to 47 months postoperatively. The present study documents complete corneal wound healing 66 months after radial keratotomy.

Adult↗
PubMed1987

Ne-like Ca XI-Mn

Explore the source record for details and available documents.

Journal Article↗
PubMed1987

Leiomyoepithelioma of iris pigment epithelium.

A 4-year-old boy had a jet-black, nodular lesion growing in his right iris. Detailed clinical and histopathologic studies of this unusual tumor showed the lesion to be derived from the anterior iris pigment epithelium. The tumor cells appeared to be benign and exhibited characteristics of both pigment epithelium and smooth muscle. We have called this tumor "leiomyoepithelioma of iris pigment epithelium."

Child, Preschool↗
PubMed1987

Application of homologous fibronectin to persistent human corneal epithelial defects.

Six eyes with persistent corneal epithelial defects of various etiologies were treated in a randomized, prospective, double-masked study to evaluate the effect of fibronectin on corneal epithelial wound healing. Study eyes received homologous human fibronectin (300 micrograms/ml), mixed with gentamicin and artificial tears, 4 times a day. Control eyes received the mixture of gentamicin and artificial tears. If no healing occurred after 2 weeks, the drug was unmasked and where control drops had been given, the mixture containing fibronectin was administered in open-label fashion. All 4 eyes in diabetic patients had successful epithelial healing when treated with the fibronectin solution. The 2 remaining corneas, which had defects secondary to a thermal injury and a presumed infectious etiology, did not heal with fibronectin.

Adult↗
PubMed1986

Nanophthalmic sclera. Morphologic and tissue culture studies.

We performed morphologic and tissue culture studies on scleral tissues obtained from a nanophthalmic patient. Seen by light and electron microscopy, collagens in these unusually thick scleral tissues were arranged in irregularly interlacing bundles. The size of the collagen fibers was more variable than that observed in the controls. These abnormal fibers appeared twisted and, in some areas, were more closely packed. In tissue culture, scleral cells derived from the nanophthalmic patient synthesized proteins and collagen at a rate similar to that of normal control cells. The level of glycosaminoglycan produced, however, was markedly reduced. The modified glycosaminoglycan metabolism in scleral cells may be related to the abnormal packing of collagen bundles, which may in turn contribute to the thickening of sclera and the formation of nanophthalmos.

Adult↗
PubMed1986

Collagen staining in corneal tissues.

We adapted a previously described procedure to quantitate collagen in corneal tissue sections prepared from paraffin-embedded samples. The method entailed staining the deparaffinized tissue sections with Sirius red, eluting the bound dye with NaOH-methanol, and estimating the color in a spectrophotometer as an indication of the collagen content. This simple, rapid and reproducible method is comparable to biochemical assays and can be applied to study various corneal specimens readily available from eye pathology laboratories. We examined corneal sections from patients with aphakic bullous keratopathy, pseudophakic bullous keratopathy, Fuchs' dystrophy and lattice corneal dystrophy with this method. No significant difference in collagen staining was found between these pathologic specimens and normal control tissues. Biochemical assays also confirmed these findings. Sections from patients with macular and granular corneal dystrophies showed reduced staining suggesting a possible alteration in collagen content. This possibility, however, was not supported by data from biochemical analysis.

Collagen↗
PubMed1985

Nodular adenomatosis of iris pigment epithelium.

A 62-year-old man manifested multiple nodular growths in his left iris and unilateral glaucoma. Detailed clinical and pathologic studies showed that these lesions were adenomas derived from the spurs of Fuchs, Michel, and Grunert in the iris pigment epithelium. We suggest terming this newly described entity nodular adenomatosis of the iris pigment epithelium and propose a classification of primary neoplastic lesions of the iris.

Adenoma↗
PubMed1985

Circinate-pattern interstitial keratopathy in daily wear soft contact lens wearers.

Three male and two female patients (aged 16 to 22 years) developed bilateral deep corneal stromal vascularization and circinate-pattern lipid exudation resembling interstitial keratitis after wearing adequately fitting hydrogel daily wear soft contact lenses comfortably for periods of ten to 24 months. The circinate pattern consisted of lipid deposition in circular distribution either parallel to the limbus or around a deep stromal blood vessel. All had negative serologic test results for syphilis. The cause of these changes is uncertain.

Adolescent↗
PubMed1985

RNA metabolism in cultures of corneal stromal cells from patients with keratoconus.

Total cellular RNA was extracted from cultured keratoconus and normal human corneal stromal cells. The translational activity of these RNAs was examined in a cell-free translation system derived from reticulocyte lysate. Results indicated that keratoconus cells can be separated into two groups, as has been shown previously. Group I keratoconus cells contained the same amount of total RNA as normal cells. RNA activity and the rate of mRNA synthesis in this group of keratoconus cells were also normal. By these criteria it seems that the protein synthesizing system is functioning properly, and group I keratoconus cells should have a normal rate of protein synthesis. These results correlate well with previous findings. Group II keratoconus cells, in contrast, contained more RNA than normal cells. The translational efficiency of RNA was so markedly reduced that the elevation in RNA content did not compensate for the decrease in translational efficiency. It is likely that the reduced protein and collagen synthesis in this group of cells is related to the reduction in the RNA activity. An inhibitory component was present in the keratoconus RNA which affected synthesis of all proteins and suppressed translation of normal RNA.

Adolescent↗
PubMed1985

Lysis of herpes simplex virus (HSV) infected targets. III. Effect of HSV on natural killer activity.

Freshly isolated peripheral blood lymphocytes from patients (PBL-P) with recurrent herpetic corneal and skin lesions, and controls (PBL-C) with no recollection of herpetic disease, effectively lysed herpes simplex virus type 1 (HSV-1) infected but not uninfected allogeneic fibroblasts. However, after 48 hr of stimulation with HSV-1, the lytic activity of PBL-C declined, while that of PBL-P increased. PBL-P and PBL-C produced similar amounts of interferon when stimulated with HSV-1. Thus, the decline in lytic activity by HSV-1 stimulated PBL-C was not due to a lack of interferon production. Interestingly, the lytic activity of HSV-1 stimulated PBL appeared to be directed against a component commonly expressed on both uninfected and HSV-1 infected fibroblasts. This was indicated by the fact that after incubation with HSV-1, PBL-P lysed the uninfected fibroblasts as effectively as the HSV-1 infected fibroblasts. The augmented lysis of uninfected fibroblasts was not due to infection of the "uninfected fibroblasts" by HSV-1 carried over or produced by the HSV-1 stimulated PBL. Furthermore, the capacity of HSV-1 to augment lytic activity was not diminished markedly by ultraviolet irradiation, suggesting that augmentation is due to the stimulatory capacity rather than the infectivity of the virus. Our data show that interaction of PBL-P with HSV-1 results in enhancement of nonspecific lytic activity and loss of the capacity to discriminate HSV-1 infected from uninfected allogenic fibroblasts.

Adult↗
PubMed1985

Lysis of herpes simplex virus (HSV) infected targets. IV. HSV-induced change in the effector population.

Herpes simplex virus type 1 (HSV-1) stimulation of peripheral blood lymphocytes from patients (PBL-P) with both recurrent corneal and oral-facial HSV-1 lesions results in altered natural killer (NK) activity. Freshly isolated PBL-P exhibit high lysis of HSV-1 infected allogeneic fibroblasts but low lysis of uninfected fibroblasts. After stimulation with HSV-1, PBL-P exhibit markedly increased lysis of uninfected fibroblasts such that the HSV-1 infected and uninfected fibroblasts are lysed with equal efficiency. The NK activity in freshly isolated PBL-P is mediated by effector cells that are phenotypically distinct from those responsible for the NK activity in HSV-1 stimulated PBL-P. In freshly isolated PBL-P most of the lysis of HSV-1 infected fibroblasts is mediated by lymphocytes expressing the phenotype OKM1+, OKT3+, and Leu-7+. After incubation with HSV-1, most of the lysis of HSV-1 infected and uninfected fibroblasts is mediated by lymphocytes that express the OKM1 antigen but lack detectable OKT3 or Leu-7 antigens. The change in function and phenotype appears to reflect in part the activation of Leu-7-, OKM1+, and OKT3- precursors of cytotoxic lymphocytes that are inactive in freshly isolated PBL-P.

Adult↗