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Biomedical subjects

J Smolle

Publications and source records attributed to J Smolle.

At least 199 records · Page 11Linked to original sources
PubMed1986

Langerhans cell density in epithelial skin tumors correlates with epithelial differentiation but not with the peritumoral infiltrate.

We investigated the intraepithelial density of Langerhans cells in 17 epithelial skin tumors by immunohistologic and morphometric methods. There was a significant difference between seborrheic keratosis (Langerhans cell density 431 +/- 31/mm2; normal epidermis: 378 +/- 20/mm2), basal cell carcinoma (28 +/- 6/mm2), and squamous cell carcinoma (100 +/- 21/mm2). No correlation was found between the Langerhans cell density and the number of intraepithelial T lymphocytes or the extent of the peritumoral inflammatory infiltrate. A significant inverse correlation was demonstrated between mean nuclear area of the epithelial tissue and the Langerhans cell density (r = -0.7; p less than 0.05). These data indicate that the number of Langerhans cells does not influence the extent of the antitumoral immune response. The correlation with the level of epithelial differentiation may be due to different homing conditions.

Antibodies, Monoclonal↗
PubMed1986

[Perioperative adjuvant chemotherapy with bleomycin in bronchogenic squamous cell cancer. A prospective randomized study].

Bleomycin (BLM) was given perioperatively and 3 and 6 months postoperatively in a dosage of 150 mg per course (total: 450 mg) to 37 patients with surgically resectable squamous-cell lung cancer. 37 randomized control patients with resectable squamous-cell lung cancer were compared to the chemotherapy-group. 5-year overall survival of the BLM group was 46.3%, that of the controls 40.5% (Mantel-Haenszel test; p greater than 0.05). BLM-treated stage I showed a 5-year survival of 47.9% (controls: 55%), BLM stage II 50% (controls: 33.3%), BLM stage III 40% (controls: 21.4%). The difference is not statistically significant. No severe adverse effects of therapy on either wound healing, or perioperative mortality of lung function were observed.

Bleomycin↗
PubMed1986

Pitfalls in the diagnosis of pulmonary metastases in malignant melanoma.

Solitary pulmonary lesions were detected roentgenographically in two melanoma patients during routine follow-up examinations. Melanoma metastases were suspected and the tumors were surgically removed. One turned out to be bronchogenic carcinoma, the other was found to be a chondroma. These cases indicate that in suspected solitary pulmonary melanoma metastases a surgical approach may be reasonable.

Carcinoma, Bronchogenic↗
PubMed1986

S-100-protein-positive dendritic cells in nodular vasculitis.

We investigated the distribution of S-100-protein-positive dendritic cells in 20 cases of nodular vasculitis (erythema induratum Bazin). The cells were identified by immunohistochemical demonstration in paraffin sections. S-100-protein-positive dendritic cells were present in all cases and comprised up to 11% of the infiltrating cells in lymphohistiocytic areas (mean 3.7%). Because of the dendritic shape and the association with lymphocytes the cells are considered to represent T-zone histiocytes. Our findings suggest a possible role of delayed-type hypersensitivity in nodular vasculitis.

Adult↗
PubMed1986

[Immunologic phenotyping of cutaneous lymphomas].

Monoclonal antibodies against lymphoid cell-associated antigens have contributed substantially to our understanding of cutaneous lymphomas. The distribution and function of the most important T- and B-cell antigens are summarized and their expression on cutaneous lymphomas is discussed. Monoclonal antibodies can provide a valuable diagnostic approach to supplement routine histology, especially in histologically doubtful cases.

Antibodies, Monoclonal↗
PubMed1986

[Retroviruses and the immune system].

Three examples are presented to illustrate the importance of retroviruses and associated oncogenes in neoplasms of the immune system. In adult T-cell leukemia/lymphoma the HTLV I-provirus is often integrated in the human genome on chromosome 8 adjacent to the c-myc protooncogene. Cytogenetic studies of Burkitt's lymphoma (translocation of a chromosome 8 fragment) and a case of mycosis fungoides (trisomy of chromosome 8) indicate that the same oncogene may possibly be activated not only by retroviruses, but also by chromosomal aberrations.

Acquired Immunodeficiency Syndrome↗
PubMed1986

[Classification and prognosis of cutaneous T-cell lymphomas].

Modern concepts on the classification and prognosis of cutaneous T-cell lymphomas are discussed in this paper. The full spectrum of cutaneous T-cell lymphomas is not yet known. A new classification of histomorphological types of mycosis fungoides and Sézary's syndrome in correlation with prognostic features is proposed.

Humans↗
PubMed1985

[Importance of immunohistochemistry of skin tumors].

Immunohistological demonstration of cell- and tissue-specific antigens in microscopic slides has remarkably influenced the classification of certain cutaneous neoplasms. The following markers are of particular interest in dermatopathology: cell surface antigens of lymphocyte subpopulations, intracytoplasmic immunoglobulins, intermediate filaments, neural markers, epithelial cell membrane antigens, and endothelial products. During the last years, these methods have contributed to our understanding of histogenesis and classification especially of cutaneous lymphomas, epithelial neoplasms, neurogenic tumors, and angiosarcomas.

Antibodies, Monoclonal↗
PubMed1985

Significance of non-lymphoid ('accessory') cells in malignant lymphomas and pseudolymphomas of the skin.

We investigated the co-distribution of lymphocyte subpopulations and non-lymphoid 'accessory' cells in 35 cases of cutaneous lymphoproliferative diseases (T-cell lymphoma, 10 cases; B-cell lymphoma, 17 cases; pseudolymphoma, 8 cases) using immunohistochemical methods. T-zone histiocytes and particularly Langerhans cells were abundant in all cutaneous T-cell lymphomas, but were also found in B-cell lymphomas. T-zone histiocytes were associated with T-lymphocytes, especially T-helper cells, but not with T-suppressor cells. Dendritic reticulum cells were essentially confined to well differentiated germinal centres. Macrophages occurred in both lymphomas and pseudolymphomas without definite relationship with either B- or T-cells. In malignant lymphomas of high grade malignancy, macrophages represented the only non-lymphoid cell type. Our results indicate that malignant lymphoid cells, like normal lymphocytes, require definite micro-environments which are, at least in part, maintained by certain non-lymphoid cells.

Antigen-Presenting Cells↗
PubMed1985

T-zone histiocytes in granulomatous skin diseases.

We investigated the distribution of T-zone histiocytes by immunohistochemical demonstration of S-100 protein in granulomatous skin diseases (granuloma annulare, 8 cases; necrobiosis lipoidica, rheumatoid nodule, sarcoidosis, lupus vulgaris, and foreign-body granuloma; 5 cases each). T-zone histiocytes were regularly found in the lymphohistiocytic mantle, but also occasionally between epitheloid cells. Our results show that, besides the monocyte-macrophage system, T-zone histiocytes consistently contribute to the formation of cutaneous granulomas. These findings may indicate a role of delayed-type hypersensitivity.

Foreign-Body Reaction↗
PubMed1985

Inflammatory cell types in normal human epidermis--an immunohistochemical and morphometric study.

Inflammatory cells in 15 specimens of normal human epidermis were selectively stained by a monoclonal antibody immunoperoxidase technique. The quantitative assessment using an interactive image analysis system revealed OKT 11 positive cells (T lymphocytes) and Leu 2a positive cells (suppressor/cytotoxic cells). As there was no significant difference in the distribution of these markers, helper/inducer cells obviously are not present in considerable amounts. OKM 5 positive cells outnumbered OKM 1 positive cells, indicating the presence of a OKM 5+, OKM 1-macrophage subset. The epidermal dendritic cells clearly showed a striking heterogeneity regarding the expression of HLA-DR (62% of OKT 6-positive cells) and Leu 3a (47%), suggesting the existence of immunologically distinct subsets of human epidermal dendritic cells.

Antibodies, Monoclonal↗
PubMed1985

HLA-DR-antigen bearing keratinocytes in various dermatologic disorders.

In normal human epidermis HLA-DR-antigen is only present on Langerhans' cells and the acrosyringeal epithelium. We investigated the distribution of HLA-DR-antigen in 78 specimens of various skin diseases by an immunoperoxidase method using a monoclonal anti-HLA-DR antibody. HLA-DR-antigen bearing keratinocytes were not only found in lichen planus and mycosis fungoides, as it has been referred previously, but were also observed in some cases of cutaneous B-cell lymphoma, pseudolymphoma, lupus erythematosus, parapsoriasis en plaque, bullous pemphigoid, drug reaction, contact dermatitis, actinic keratosis and verrucous carcinoma. Direct contact of lymphoid cells with keratinocytes was not necessary for Ia-antigen expression.

Antibodies, Monoclonal↗
PubMed1985

[Therapy of pemphigus. Critical remarks based on 44 clinical cases].

The results are evaluated of therapy administered between 1957 and 1983 to 44 patients with pemphigus (28 pemphigus vulgaris, 5 pemphigus vegetans, 11 pemphigus seborrhoicus). The mean initial steroid dosage was 87 mg prednisone equivalent per day. The 5-year survival rate was 83%. Fifteen patients who were additionally treated with azathioprine during the initial phases of the disease had a 5-year survival rate of 100%. However, after having the disease for 5 years 41% of all pemphigus patients have to be rehospitalized. This percentage is only 21% in the group requiring less than 100 mg prednisone per day initially. Mortality related to pemphigus or infection occurs significantly earlier than cardiovascular deaths. The prognosis for survival is relatively good, but the prognosis for recovery remains uncertain. Combined therapy with corticosteroids and azathioprine in the early stages of the disease resulted in a 5-year survival rate of 100%, and is therefore recommended. However, even this therapeutic modality does not change the uncertain prognosis regarding recovery.

Adrenocorticotropic Hormone↗
PubMed1984

[Histological technics in dermatology].

Apecial histotechnical problems with reference to dermatology are reviewed. Analysis of the physical and chemical conditions helps to avoid common failures.

Chemical Phenomena↗