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J Sander

Publications and source records attributed to J Sander.

At least 73 records · Page 4Linked to original sources
PubMed1985

Screening for rubella IgG and IgM using an ELISA test applied to dried blood on filter paper.

IgG and IgM rubella antibodies were measured in dried blood samples from the neonatal metabolic screening program. Of 6613 samples from four central screening laboratories, 289 did not contain rubella IgG antibodies, indicating that only 4.3% of the mothers at term were not immune to the rubella virus in the spring of 1984. The proportion of women at term who were not immune was 9.3% in 1979. Rubella IgM was detected in nine of 37,000 samples; congenital rubella infection was confirmed serologically in eight infants. Of the infected babies, three showed severe clinical findings. We suggest that rubella IgG and IgM should be determined regularly in the surplus material of metabolic screening centers, thus giving the opportunity to detect infected infants. The screening procedure is also useful as a serologic tool for monitoring immunity patterns in women, and thus the effectiveness of vaccination programs.

Antibodies, Viral↗
PubMed1984

[The IgM enzyme immuno-screening test for congenital rubella].

A commercially available rubella-IgM-enzyme immunological assay (Enzygnost, Behringwerke) was used to test 20 000 samples for rubella-specific IgM antibodies. Examination of dry-blood samples on filter paper avoided any false-positive or doubtful readings. In five children congenital rubella infection was detected by definitely positive test results. In three first clinical examination was unremarkable, two had cardiac and other abnormalities. Two of the children were of mothers in whom positive results of serological screening for rubella had been misinterpreted as sufficient protection against rubella. These results favour the inclusion of IgM-specific tests in a screening programme. Because of possible late sequelae of the infection, symptom-poor children should be carefully examined and followed for several years.

Female↗
PubMed1984

Pyruvate carboxylase activity in subacute necrotizing encephalopathy (Leigh's disease).

Leigh's disease is a heterogeneous group of disorders, in which clinical and biochemical features suggest abnormal pyruvate metabolism. In two patients with Leigh's disease, diagnosed according to rigorous clinical, radiographic, and histologic criteria, we tested the hypothesis that pyruvate carboxylase deficiency might be the primary etiology. Pyruvate carboxylase specific activities in extracts of cultured skin fibroblasts from both patients were in the normal range. These results, together with other evidence, suggest that isolated pyruvate carboxylase deficiency does not cause the Leigh's disease phenotype.

Brain Diseases↗
PubMed1984

Plasma levels of the leucocyte L1 protein in febrile conditions: relation to aetiology, number of leucocytes in blood, blood sedimentation reaction and C-reactive protein.

Plasma levels of a novel leucocyte protein (L1) were determined in a series of 176 febrile patients and compared with CRP, BLC, and BSR. Among 82 patients with bacterial infection, 81 had L1 levels above the 0.95 interval. Levels of 40 to 130 times the normal mean were seen frequently during life-threatening infections like septicaemia, meningitis, or pneumonia. In contrast, 23 of 27 patients with viral infections had normal L1 levels. A poor correlation was found between L1 levels, CRP, BLC, and BSR, suggesting that these parameters reflect different aspects of the response to tissue damage and inflammation. L1 and CRP seem equal in distinguishing between bacterial and viral infections. Low or normal L1 levels argue strongly against a bacterial infection, while elevated L1 levels discriminate poorly between bacterial and non-infectious inflammatory or malignant diseases.

Adolescent↗
PubMed1984

Late sequelae after meningococcal disease. A controlled study in young men.

The occurrence of sequelae 3-15 years after meningococcal disease has been investigated in a study on 71 patients and 64 controls. The patients were young men, aged 18 to 24 years at the time the disease was contracted. Participants filled in a questionnaire on possible symptoms. Audiometry and EEG were also carried out. The response rates were 84% among patients and 75% among controls. We found that 61% of the patients had one or more symptoms of possible sequelae compared to 20% in the control group (p less than 0.001). The symptoms were generally light and of mental or neurological type. Among the patients 13% stated that they had obvious complaints commonly attributed to meningococcal disease, compared to 2% only in the controls (p less than 0.05). Twenty-nine per cent of the patients stated that the disease had affected their education or working capacity. No statistical differences between patients and controls were demonstrated by audiological or EEG examinations. In only one single ear could deafness unequivocally be attributed to the disease.

Adolescent↗
PubMed1984

[Determination of free thyroxine (FT4) for the detection of connatal hypothyroidism within the scope of neonatal screening].

Using the reagents of a commercially available test kit (Henning, Berlin) for the determination of free thyroxin (FT4) in serum we developed a radio immuno assay procedure to measure FT4 in whole dried blood on filter paper. The inter assay coefficient of variation was 19.1%, 10.7%, and 11.0% for the hypo-, normo-or hyperthyroid range for whole blood on filter paper. The corresponding within assay values were 7.0, 10.3, and 5.3% respectively. In 15,793 samples of dried blood on filter paper which were collected on the 5th or 6th day of life for the screening of inborn errors of metabolism or hypothyroidism FT4 was measured in addition to our routine determination of thyroid stimulating hormone (TSH). The mean concentration of FT4 was 20 to 22 pg/ml. 97,8% of all results were found between 10 and 35 pg/ml. Connatal hypothyroidism was confirmed clinically in 5 children showing FT4-values of 3.5, less than 2.0, less than 2.0, 3.4, and 6.2 pg/ml. The corresponding TSH-values were greater than 224, greater than 224, 99, 203, and 129 microU/ml. To make sure that a sufficient amount of blood had been eluted from the filter paper we measured the concentration of hemoglobin as an additional parameter. Low FT4 together with low hemoglobin concentrations were obtained in 18 samples indicating that a mismeasurement of FT4 might have occurred. We suggest that the determination of TSH in doublicates, which is the routine screening procedure in West Germany might be replaced by a combined determination of FT4 and TSH.

Congenital Hypothyroidism↗
PubMed1984

Late sequelae after meningococcal disease as related to anamnestic and clinical factors recorded during the acute illness.

In 71 males who survived acute meningococcal disease 3 to 15 years ago at an age of about 20, associations between acute clinical conditions (including a few pre- and post-admission variables) and late sequelae have been studied. There was a higher rate of sequelae symptoms (mainly light neurological and mental disturbances) among survivors from meningitis (76%) than among those who had had both meningitis and septicemia (58%) or pure septicemia (50%). Twenty percent of control persons experienced such symptoms. "Changed Life" because of serious educational and working problems followed in 29% of the meningitis cases and 70% of the septicemia cases. Most of the clinical and laboratory factors separately examined were not significantly correlated to the sequelae rates. However, less than 2.5 mmol/l glucose in the cerebrospinal fluid (CSF) on admission (p less than 0.01), more than 1000 X 10(6) white blood cells per 1 in the cerebrospinal fluid (p less than 0.05), fever for more than 8 days (p less than 0.05), and probable cerebral symptoms the first week (p less than 0.05), were all positively correlated to a high rate of late sequelae. Well documented early sequelae correlated with serious late sequelae (p = 0.05). No conspicuous associations between acute antibiotic treatment and late sequelae were found. A combination of CSF glucose, blood thrombocytes, and cells in CSF on admission yielded a multiple regression score which seems to be a moderately reliable predictor of sequelae (R = 0.46). Hospital treatment should both aim at avoiding death and escaping residual effects. Because many prognostic factors for sequelae on admission are different from those for lethality, scoring for sequelae may be helpful in such secondary prevention of sequelae. Early standardized registration of sequelae may also be of value in tertiary prevention.

Acute Disease↗
PubMed1983

[Incidence of primary toxoplasmosis infection in pregnant women].

Toxoplasma-specific antibodies transferred across the placenta were determined in 1953 blood samples of newborn infants from the Lower Saxony newborn-screening-laboratory using the indirect immunofluorescence test. There were no antibodies demonstrable in 54.4% of the samples. Related to the age of the mothers there was increase of positive findings of about 1% per year. Among 10 000 pregnancies about 70 to 80 mothers can be expected to undergo a primary infection with Toxoplasma gondii. With a fetal infection rate of about 50% derived from the literature 30 to 40 cases of congenital toxoplasmosis of variable severity, three among them serious, may be expected.

Antibodies↗
PubMed1983

[Tube feeding].

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Humans↗
PubMed1983

[Rubella immunity in pregnant women in 1983].

The measurement of diaplacentally transferred specific antibodies in 2153 samples of dried blood of our neonatal screening programme for the detection of inborn errors of metabolism showed that only 4.3% of the children's mothers lacked immunity to rubella in March 1983. This demonstrated a substantial improvement as compared with 1979 and 1982 when 9.3 and 5.3% of the mothers were susceptible to infection with the rubella virus. The reimbursement of serological testing by the legal insurance companies and the vaccination in case of negative results were considered instrumental in reducing the susceptibility to rubella.

Adolescent↗