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J Ruffié

Publications and source records attributed to J Ruffié.

At least 19 recordsLinked to original sources
PubMed1995

Structural analysis of the RH-like blood group gene products in nonhuman primates.

Rh-related transcripts present in bone marrow samples from several species of nonhuman primates (chimpanzee, gorilla, gibbon, crab-eating macaque) have been amplified by RT-polymerase chain reaction using primers deduced from the sequence of human RH genes. Nucleotide sequence analysis of the nonhuman transcripts revealed a high degree of similarity to human blood group Rh sequences, suggesting a great conservation of the RH genes throughout evolution. Full-length transcripts, potentially encoding 417 amino acid long proteins homologous to Rh polypeptides, were characterized, as well as mRNA isoforms which harbored nucleotide deletions or insertions and potentially encode truncated proteins. Proteins of 30-40,000 M(r), immunologically related to human Rh proteins, were detected by western blot analysis with antipeptide antibodies, indicating that Rh-like transcripts are translated into membrane proteins. Comparison of human and nonhuman protein sequences was pivotal in clarifying the molecular basis of the blood group C/c polymorphism, showing that only the Pro103Ser substitution was correlated with C/c polymorphism. In addition, it was shown that a proline residue at position 102 was critical in the expression of C and c epitopes, most likely by providing an appropriate conformation of Rh polypeptides. From these data a phylogenetic reconstruction of the RH locus evolution has been calculated from which an unrooted phylogenetic tree could be proposed, indicating that African ape Rh-like genes would be closer to the human RhD gene than to the human RhCE gene.

Amino Acid Sequence↗
PubMed1994

Molecular genetics of chimpanzee Rh-related genes: their relationship with the R-C-E-F blood group system, the chimpanzee counterpart of the human rhesus system.

As the chimpanzee R-C-E-F blood group system appears to be the chimpanzee counterpart of the human Rhesus (RH) system, we have tried to determine whether chimpanzee Rh-like genes encode R-C-E-F-related proteins. Chimpanzee genomic DNA, digested by any of eight endonucleases and hybridized with three Rh exon-specific probes, exhibits a high degree of polymorphism. Analysis of DNA from unrelated individuals of different R-C-E-F types revealed that the presence of some restriction fragments is correlated with particular R-C-E-F types. The cosegregation of these fragments with R-C-E-F haplotypes was confirmed by family studies. Oligonucleotides complementary to regions flanking human exons were used as PCR primers on chimpanzee DNA; the resulting amplified fragments were identical in size to their human counterparts. Moreover, the nucleotide sequences of the fragments present a high degree of similarity to the corresponding human regions.

Animals↗
PubMed1994

Length polymorphism of a microsatellite in human and non human primates.

Microsatellites are tandem repeats of short sequences elements (most often CA repeats) interspersed in many genomes and which frequently show multiallele polymorphism. They have proved invaluable for genomic mapping in man and other species and may be used for evolutionary studies provided that the available primers can be used in different species. The dystrophin gene, which shows high sequence conservation between man, rodents and chicken contains such polymorphic CA repeats. Using the oligonucleotides primers developed for testing the polymorphic CA repeat of the 3'untranslated region of the dystrophin gene in man, we achieved the amplification by polymerase chain reaction (PCR) of the analogous region in five non human primates species (Pan troglodytes, Gorilla gorilla, Macaca tonkeana, Macaca fascicularis, Lemur fulvus). All were proved to possess the CA repeat while intraspecies variations of the microsatellite length was observed in chimpanzees, gorillas and tonkean macaques. As it was demonstrated by sequencing, these length variations depend on the number of CA repeats in the microsatellite.

Animals↗
PubMed1993

Comparative study of human monoclonal anti-D antibodies of IgG and IgM classes in tests with red cells of nonhuman primates.

Forty-nine human anti-D (Rho) monoclonal antibodies of the IgG and IgM classes were tested with red blood cells of various nonhuman primates, from anthropoid apes to Prosimians, and significant differences in reactivity were observed among antibodies of two classes depending on taxonomic position of primate animals. By and large, higher percentage of IgM mAbs gave positive reactions with nonhuman primate red cells and, particularly, with blood of lower monkeys: Old and New Worlds monkeys and Prosimians, than did those of IgG class. Allotypic reactions with red cells of African apes were produced by majority of IgG mAbs but by very few IgM reagents. Some of those reactions defined epitopes related to human D, such as chimpanzee Rc and gorilla Dgor. By contrast, individual differences among Old World monkey species were revealed almost exclusively in tests using anti-D mAbs of IgM class. Some of the epitopes detected by these antibodies on the red cells of macaques are related to human D alloantigen, as confirmed by absorption experiments. Differences among mAbs evidenced in tests with nonhuman primate red cells reflect the complexity of the immune reactions to the human D antigen.

Animals↗
PubMed1993

The R-C-E-F blood group system of chimpanzees: serology and genetics.

Six chimpanzee alloimmune antibodies define 20 phenotypes of the R-C-E-F blood group system, the counterpart of the human Rh system. Of the several specificities of this system, the Rc constitutes the crucial link with human Rh since the reactions of some chimpanzee alloimmune anti-Rc sera with human red cells parallel those obtained with human anti-Rho reagents. Reciprocally, properly absorbed human anti-Rho sera detect Rc specificity on chimpanzee red cells. Tests with large panels of human monoclonal anti-D antibodies confirm the notion of shared epitopes between human alloantigen Rho(D) and chimpanzee alloantigen Rc.

Alleles↗
PubMed1993

Quantitative study of chimpanzee and gorilla counterparts of the human D antigen.

The reactivities of three human anti-D monoclonal antibodies (mAbs) with human, chimpanzee, and gorilla red blood cells (RBCs) were compared by quantitative radioimmunology and indirect immunofluorescence methods. The number of antigenic sites varies widely in gorillas (from 48,000-283,000), while in chimpanzees this number is very close to that observed in human R1R2 RBCs. The affinity of the anti-D antibodies was slightly lower with ape RBCs than with D-positive human RBCs. In chimpanzee, the D-like epitopes recognition is enhanced by papain while the gorilla D-like epitopes are destroyed by enzyme treatment.

Animals↗
PubMed1993

Relationship between chimpanzee Rh-like genes and the R-C-E-F blood group system.

The antigenic closeness between the chimpanzee alloantigen Rc of the R-C-E-F system, and the human alloantigen Rho(D) suggests a phyloge-connection between their genes. To confirm at the molecular level the common origin of these genes, genomic DNA from 16 unrelated chimpanzees of various R-C-E-F phenotypes were digested by three restriction enzymes and analyzed by Southern blot using a human Rh cDNA probe and three exon-specific probes. Restrictions profiles displayed reach polymorphism. Correlations between some bands and certain R-C-E-F phenotypes demonstrate that the human Rh cDNA probe defines in chimpanzee genomic DNA some genes of the R-C-E-F system.

Animals↗
PubMed1993

Immunoglobulin Gm allotypes in apes: comparison with man.

Serum samples from 245 apes (184 Pan troglodytes, five Pan paniscus, 28 Gorilla gorilla, 23 Pongo pygmaeus abelei, and five Pongo pygmaeus pygmaeus) were tested for G1m (1,2,3,17), G2m (23), and G3m (5,6,10,11,13,14,15,16,21,24,28) immunoglobulin allotypes by the classical method of inhibition of hemagglutination. Some phenotypes are species specific while a few are shared by man and African apes.

Animals↗
PubMed1992

[DNA polymorphism in a Brazilian population: relationship to allele frequencies in France].

The allele frequency distribution of two highly polymorphic DNA sequences was determined in a population from the metropolitan area of Fortaleza (Northeast Brazil). DNAs from 234 individuals were digested with Pvull and successively hybridized to each probe: alpha-Globin 3'HVR and Mucin HVR. The resulting allele frequencies were compared to those observed in a French population. There is no statistically significant difference between these populations in the 3'HVR system. On the contrary, the distribution is statistically different in the Mucin HVR/Pvull system.

Alleles↗
PubMed1992

[Study of equivalents of rhesus antigens in non-human primates].

Human alloantibodies specific of some Rh antigens cross-react with non human primates red blood cells. These crossreactions demonstrated that only African apes express equivalents of Rho (D) and hr' (c). The antigenic resemblance between these two human antigens and their primate homologues is confirmed by the reactivities of human anti-D and anti-c monoclonal antibodies. The use of a human Rh cDNA probe allowed to confirm by Southern blot hybridization that nonhuman primates possess Rh-like genes. The number of Rh-like genes per haploid genome was deduced from the results obtained with exon-specific probes.

Animals↗
PubMed1990

The p49/TaqI Y-specific polymorphisms in three groups of Indians.

The TaqI/p49 Y-specific RFLPs were studied in 98 Indians coming from 3 locations in the country. A new allele (G0) and five new haplotypes (XX-XXIV) were found, not present in Caucasians and in Africans. In the genealogy of haplotypes, the new Indian haplotypes appear grouped together, and all Indian haplotypes occupy an intermediate position between Caucasian and African haplotypes.

Alleles↗
PubMed1989

Complexity of the Rh antigen demonstrated by comparative tests using antisera of human and primate origins.

Comparative analysis of two antisera, one produced in chimpanzee and another of human origin, demonstrates the existence of the whole spectrum of antibodies directed against at least four, and possibly five, antigenic determinants connected with the Rh reactivity. Some of the determinants are shared by chimpanzee and human red cells, while others are restricted to one species only. Based on this study, it is suggested that both the human Rh(D)-positive type and its chimpanzee counterpart, the Rc-positive type, could be of common origin, while the negative types are the results of later, parallel events during the evolution.

Animals↗
PubMed1987

[Polymorphism of p49 Y-specific probe in Papuas Baruyas of Papua New Guinea].

Taq I polymorphism revealed after molecular hybridization with the Y-specific p49 probe was studied in male Papuas, Baruya tribe, living in the Wonenara valley. All individuals screened were identical at the variable loci A, C, F and I, and fixed for the specific allele Db. The deduced haplotype, number 17 (A2, C0, Db, F1, I1) is Baruya-specific.

Alleles↗
PubMed1983

An analysis of the blood group composition of a population in Brittany, the Bigoudens.

Data are presented on the blood groups of the Bigoudens. Deviations from the expected Hardy-Weinberg distribution are interpreted as the result of a recent mixing of previously separated groups, in addition to the effect of silent alleles and inbreeding. Special reference to the historical evidence of their origin is made.

ABO Blood-Group System↗
PubMed1983

Red cell enzyme polymorphism in a Breton population, the Bigoudens.

Data are presented on the red cell enzymes of the Bigoudens. Deviations fro the expected Hardy-Weinberg distribution are interpreted as the result of a recent mixing of previously separated groups, in addition to the effect of silent alleles and inbreeding. Special reference to the historical background.

Acid Phosphatase↗