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Biomedical subjects

J Rozniecki

Publications and source records attributed to J Rozniecki.

At least 19 recordsLinked to original sources
PubMed1997

[The influence of multiple histamine inhalation on nonspecific airway hyperresponsiveness to histamine in patients with bronchial asthma].

The study was performed in 19 patients with stable mild/moderate asthma aged from 16 to 66. Nonspecific airway hyperresponsiveness (AH) to histamine was measured according to Cockcroft's et al. method and expresses as PC20H in mg/ml. The study was controlled by inhalation of PBS. The histamine or PBS challenges were performed three times at 45 min. intervals on the third day in case when the stability of AH on two preceding days was observed. The geometric means of PC20H (xg PC20H) during the consecutive three days did not change. They were 0.97, 0.92, 0.87 mg/ml (p > 0.05) respectively. Three times histamine challenges performed on the same day induced the decrease of AH (xg PC20H increased from 1.48 to 6.55 mg/ml) in 5 patients. In 4 other subjects the increase of AH was observed (xg PC20H decreased from 1.14 to 0.20 mg/ml). In 10 subjects the three times histamine challenges performed on the same day had no influence on AH to histamine.

Administration, Inhalation↗
PubMed1997

[An increase of nonspecific airway hyperreactivity after repeated challenge with subthreshold doses of allergen in asthma patients].

The study was performed in 11 patients suffering from stable mild and moderate atopic asthma. Bronchial allergen challenges were performed according to Chai et al method. Airway hyperreactivity-AH (PC20H in mg/ml) was determined in each study day before subthreshold allergen inhalation dose, meaning the dose which did not induce asthmatic responses. Then FEV1 was measured in 2, 5, 15, 30 i 60 min. after allergen provocation throughout 4 to 9 consecutive days. A significant increase of PC20H was observed in 8 patients (xg PC20H in whole group decreased from 0.93 to 0.35 mg/ml after allergen challenges). The authors conclude that allergen inhalation challenge without asthmatic bronchial responses can cause an increase AH.

Adult↗
PubMed1997

[Complications after BCG vaccination in the urban section of Lodź in the years 1994-5].

The purpose of this paper was to evaluate the incidence of complications after BCG vaccination in children from urban area of Lódź in 1994-1995 and to give their pathological and prognostic interpretation on the basis of immunological and Groër allergometric examinations. The obtained data demonstrate that postvaccinal complications occurred in 46 children, that is 0.7/1000 of vaccinated population. They were observed mainly in newborns (45.7%), whereas they were particularly rare in revaccinated six-year-old children (13%) and schoolchildren (8.7%). In half of the cases there was an evidence of ulceration and suppuration in the site of vaccination, in another half--suppuration of local lymph nodes with or without fistula. Immunological and allergometric examinations were carried out in 21 children with post-vaccinal complications and 21 children with normal post-vaccinal period. In both groups the following were the subjects of evaluation: values of B and T lymphocytes and their CD4 and CD8 subpopulations, lymphocytes proliferative response to mitogenic PHA doses and tuberculin, as well as IgG, IgA and IgM levels. Immunological and allergometric examinations indicated that immunosuppression was neither the cause nor the effect of BCG complications.

Adolescent↗
PubMed1997

[The effect of nedocromil sodium on spontaneous production of histamine releasing factor (HRF) by mononuclear blood cells in patients with nonatopic bronchial asthma].

The aim of the study was to evaluate the effect of 28-days antiinflammatory treatment with nedocromil sodium (Ned.sod.) (Tilade-8 mg/24 hours) on nonspecific bronchial hyperreactivity (PC20H in mg/ml) and spontaneous HRF activity production by mononuclear blood cells in patients with nonatopic bronchial asthma treated with beclomethasone dipropionate. The correlation between PC20H and HRF was also examined. The study was performed in 10 subjects with mild and moderate chronic, stable asthma in a double-blind, placebo-controlled way. Placebo and Ned. sod were given through 4 weeks in a randomized way with 8 weeks wash-out period. It was shown that Ned. sod. did not influence clinical asthma symptom scores, ventilatory parameters and PC20H. No changes in the spontaneous production of HRF activity were observed. The correlation between HRF activity and PC20H assessed 4 times was not significant. The authors conclude that studies with Ned. sod. in nonatopic asthma should be continued, but the dosage of the drug ought to be bigger and the time of treatment should to be longer.

Adult↗
PubMed1996

[Chemotactic serum activity for neutrophils and eosinophils and spontaneous production of histamine releasing factor in patients with seasonal bronchial asthma treated with nedocromil sodium].

Nedocromil sodium (NS- Tilade) is an effective therapeutic agent against asthma and has been shown to exhibit antiinflammatory activity in vitro. The aim of this study was to assess the effect of NS on the serum neutrophil (NCA) and eosinophil (ECA) chemotactic activity and mononuclear cells-derived histamine releasing factor (HRF) activity in 14 patients with seasonal allergic asthma. The chemotactic activities, HRF and bronchial reactivity to histamine (PC20H in mg/ml) were determined before the grass-pollen season and at the end of the 14-day placebo treatment and 21-day NS treatment during the pollen-season. NCA and ECA were assessed using of the Boyden chamber methods. Blood samples for mononuclear cells culture were collected during the trial. The supernatants were assayed for HRF activity with basophils from a single donor. The NCA index was unaltered during the trial. There was a significant (p < 0.05) increase in the ECA after the NS treatment period (ECA index--2.5) compared with out of season and after placebo treatment (ECA index, 1.88 and 1.82). HRF activity increased after placebo compared with out of season (HRF % activity--38.62 before season, 47.61 after placebo p < 0.05) but NS did not revealed the further effect on HRF activity (49.81 p > 0.05). During placebo the significant decrease in PC20H from 1.81 mg/ml to 0.54 mg/ml (p < 0.05) was observed. NS did not have the effect on nonspecific bronchial reactivity--PC20H--0.74 mg/ml (p > 0.05). The correlation between PC20H and spontaneous production of HRF activity was not found. The results indicate that NS has some effect on inflammatory process which takes place in seasonal asthmatic patients during the natural allergic exposure.

Adolescent↗
PubMed1996

[Bronchial hyperresponsiveness in patients with seasonal allergic rhinitis and the influence of loratadine and beclomethasone on this hyperresponsiveness].

60 patients aged 16 to 46 years with seasonal allergic rhinitis were selected for study. Daily symptom scores of seasonal allergic rhinitis and nonspecific bronchial hyperresponsiveness to histamine (PC20H in mg/ml) before, during the pollen season and after 3 weeks of treatment with loratadine, beclomethasone dipropionate were evaluated. The control group received oxymetazoline. Nonspecific bronchial hyperresponsiveness for 11 patients (18.3%) before the seasons was observed. At the beginning of the season frequency of nonspecific bronchial hyperresponsiveness increased to 26.3% and to 36.8% after 3-week treatment course. Bronchial hyperresponsiveness was not related to any of the way of treatment. The patients treated with beclomethasone dipropionate and oxymetazoline showed significant relief of nasal symptoms. The patients without bronchial hyperresponsiveness showed lower value of symptom scores.

Adolescent↗
PubMed1996

[The effect of bronchial allergen provocation on spontaneous production of histamine releasing factor (HRF) by mononuclear cells in patients with bronchial asthma].

The study was performed in 8 patients suffering from mild asthma. Bronchial allergen challenges with Dermatophagoides pretonyssinus were performed according to Chai et al method. Nonspecific bronchial reactivity to histamine (PC20H in mg/ml) were determined before the allergen challenge and in 90 min. and 24 hrs. after allergen provocation. Blood samples for mononuclear cells culture were collected before the trial and after the allergen challenge in 15, 90 min. and 8 and 24 hrs. The supernatants were assayed for HRF activity with basophils from a single donor. The significant increase of PC20H was observed xgPC20H in mg/ml 0.43 before allergen challenge and 0.08 and 0.125 after allergen provocation. Blood samples did not reveal any significant changes in HRF % activity--mean and SD before challenge test 47.7 16.2 and 49.9 15.0 in 15 min, 53.3 19.0 in 90 min, 56.6 15.8 in 8 hr, 52.5 20.4 in 24 hr after allergen provocation. The correlation between postallergen, nonspecific bronchial reactivity and spontaneous production of HRF activity was not found. The authors discuss the role of HRF in pathogenesis of nonspecific bronchial reactivity of asthmatic patients.

Adult↗
PubMed1996

Changes of serum concentration of lipid peroxidation products in patients with pneumonia.

During lower respiratory tract infection, massive influx and activation of phagocytes is observed. Reactive oxygen species (ROS) released by macrophages and polymorphonuclear neutrophils (PMNs) kill microorganisms and cause damage to host tissues. One feature of this damage may be enhanced lipid peroxidation. Therefore, the aim of this study was to estimate the serum concentration of lipid peroxidation products in combination with clinical and biochemical indicators of inflammation in 32 patients with pneumonia. Serum concentration of lipid peroxides (CLP) and malondialdehyde (CMDA) was measured at Day 1, 4, 10 and 14 of observation, whilst chest radiography and routine blood analysis were performed at Day 1 and 14 during a 2 week treatment of lower airway infection. The CLP decreased during treatment (p < 0.05) from 0.059 +/- 0.024 to 0.043 +/- 0.017 (A532 nm) and the CMDA (p < 0.05) from 3.5 +/- 1.4 to 2.8 +/- 1.3 nmol.mL-1. A negative correlation between CLP and radiological regression (r = 0.49) and a drop in white blood cell count (WBC) (r = 0.39) was observed during the treatment. A positive correlation between CMDA and serum trypsin inhibitory capacity (STIC) (r = 0.47) and erythrocyte sedimentation rate (ESR) (r = 0.43) was found. Our data indicate that an enhanced process of lipid peroxidation occurs during pneumonia and that serum concentration of lipid peroxides returns to normal values quicker than the concentration of malondialdehyde during recovery. The use of antioxidants is suggested as an adjuvant treatment in patients with pneumonia.

Adult↗
PubMed1995

[Course of non-specific bronchial reactivity to histamine after bronchospasm induced by allergen challenge in patients with bronchial asthma].

Bronchial hyperresponsiveness is the characteristic feature of bronchial asthma. Inhalation of allergen can cause dual asthmatic response--early and late reaction. Thirteen patients with mild and moderate asthma underwent bronchial allergen challenge. Non-specific bronchial reactivity was measured before, 90 minutes, on the 2nd, 7th, 14th day after provocation. Peak Expiratory Flow was measured every hour during 12 hours after challenge to search for late phase reaction. Increased bronchial reactivity was discovered as early as 90 minutes after challenge, it was still observed on the 2nd and 7th but not on the 14th day after allergen provocation. No relationship was found between appearance of late asthmatic reaction and increased bronchial reactivity.

Adult↗
PubMed1995

[Effect of loratadine, selective antagonist of histamine H1 receptors, on histamine-induced bronchoconstriction].

The aim of this study was to assess the duration of Loratadine effect on histamine H1 receptor in the airway. Six patients with mild or moderate asthma were examined. Bronchial reactivity to histamine was measured according to Cockcroft. The significant blockade of histamine H1 receptors in airway was observed from the 2nd day when 10 mg of Lo was given daily. The maximal protection with 10 mg doses was observed after 3-4 days. After another 5-6 days without Loratadine bronchial responsiveness return to the initial values. Single dose of 30 mg Loratadine given better protection against histamine bronchoconstriction than 10 mg given for 5 days. This effect was observed as early as 60 minutes after administration of Loratadine.

Adult↗
PubMed1995

[Effect of tobacco smoke on serum antioxidant activity].

Oxidants of cigarette smoke and those released from phagocytes in smoker's lungs may inactivate alpha-1-antiproteinase. Thus, the "elastase-antielastase" imbalance may lead to lung tissue destruction and emphysema. Insufficient antioxidant protection, postulated by some authors might be an additional factor contributing to this process. The presented data on the influence of acute in vitro exposure to cigarette smoke in humans did not show difference in serum antioxidant activity (AOA) before and after the exposure. Attempts were made to evaluate the effect of whole cigarette smoke, its gas-phase and water-soluble phase on serum AOA in vitro. The results show that gas-phase leads to the depletion of serum AOA, whereas water-soluble phase exerts protective effect. Thus, at least part of oxidants might be inactivated in the stream of inhaled smoke. In conclusion, we doubt that serum AOA is influenced as a result of smoking and that the depletion of serum AOA is a decisive factor in the development of pulmonary emphysema.

Adult↗
PubMed1995

[Characteristics of hay fever during pollen season with regard to the influence of specific immunotherapy. I. Clinical course and biochemical changes in nasal lavage].

Nasal lavage is a useful tool for monitoring of inflammatory process in pollinosis. In 27 patients with pollen allergy the nasal lavage was performed before, during and after pollen season. The concentration of total protein, albumin and lysozyme were determined in obtained fluid. In one group Pollinex (Bencard) was applied before pollen season, and in the second one--placebo in similar injections. The concentrations of total protein in nasal lavage fluid was significantly lower after the pollen season in both analyzed groups. On the other hand, the concentrations of albumin and lysozyme were increased during pollen season relatively to values before and after the season. In patients treated with Pollinex observed values after the season were lower than in placebo group.

Adult↗
PubMed1995

[Course of hay fever during the pollen season with respect to the effect of specific immunotherapy. II. Cytologic changes and chemotactic activity in nasal lavage].

The cytological changes and chemotactic activity in nasal lavage fluid were observed in 27 patients with pollen allergy. In one group of patients Pollinex was administered, but in the second--only placebo. Total cell count in nasal secretions was increased after pollen season. The significant increase of eosinophils and metachromatic cells was observed during the season in comparison with the time before and after it. No seasonal dynamic was documented in chemotactic activity of neutrophils in the nasal lavage fluid. Applied immunotherapy gave a some protection on evaluated parameters and observed symptoms.

Adult↗
PubMed1995

[Effect of nedocromil sodium on clinical course and histamine airway responsiveness in patients with pollen induced bronchial asthma].

Nedocromil sodium is a non-steroidal prophylactic agent for the management of asthma. We assessed the effect of inhaled of nedocromil sodium 8 mg/24 h in a double blind, placebo controlled study in 14 patients suffering from seasonal (grass-pollen) asthma. Symptom scores for dyspnoe, cough, rhinitis and airway responsiveness to histamine (PC20H) according to Cockcroft et al were estimated before the grass-pollen season and at the end of 14-day placebo treatment and 21-day nedocromil sodium treatment. During placebo period the significant decrease in PC20H from 1.81 mg/ml to 0.54 mg/ml (p < 0.01) was observed. Nedocromil sodium did not have the effect on nonspecific bronchial responsiveness, PC20 - 0.74 mg/ml (p = 0.25). Subjective global assessment scores were significantly better with nedocromil sodium (31.15 mean 2.23) than with placebo (10.5 mean 0.7) p < 0.05.

Adult↗
PubMed1995

[Evaluation of selected factors induced by antioxidative activity in serum of cigarette smokers and in patients with early phase emphysema].

We investigated the concentration of ceruloplasmin (CP), transferrin (T) and metal ions (Cu, Zn) in serum of healthy smokers, emphysema patients and healty non-smoking subjects (control). We observed elevated Cu concentration in smokers and emphysema patients. Elevated CP concentration was observed only in those emphysema patients who smoked cigarettes, but not in healthy smokers. This observation excludes the possibility of compensative CP and Cu increase as the effect of chronic oxidant exposure. Changes in concentration of CP and Cu observed by us are probably due to chronic inflammation of respiratory tract not only of emphysema patients but also of chronic smokers without signs of the disease.

Adult↗