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Biomedical subjects

J Richard

Publications and source records attributed to J Richard.

At least 91 records · Page 5Linked to original sources
PubMed1990

A novel covalent enzyme-linked immunoassay (CELIA) for simultaneously measuring free and immune complex bound antibodies of defined specificity. I. Application to naturally occurring antipolyamine antibodies in human sera.

A simple covalent enzyme-linked immunoassay procedure (CELIA) is described for the routine determination of free and immune complex-bound antibodies in sera. Assays for the latter could not have been performed by adsorption ELISA due to the high ionic strength of the reassociating buffer. For the measurement in human sera of free naturally occurring IgG and IgM antibody directed against the hapten spermine, polycarboxystyrene microtiter plates with covalently coupled spermine were used. For the determination of immune complex-bound antipolyamine IgG and IgM antibody titers, serum was first dissociated at pH 2.3 in tubes and then reassociated at pH 8.1 in the wells of a microtiter plate containing covalently bound spermine. The reactivity of anti-spermine antibodies was increased from 2- to 13-fold after dissociation and reassociation compared to that of non-dissociated area. The apparent reaction constant (Rapp.) of free IgG antibodies to spermine in the sera of 19 bronchopulmonary patients with cancer differed significantly from Rapp. values of IgG antibodies having this specificity in ten other patients with non-malignant disease.

Animals↗
PubMed1990

Path of bullet and injuries determined by radiography.

Radiography is commonly used to find bullet pathways in forensic pathology. In the case presented here, a man had sustained multiple gunshot wounds and one bullet could not be traced. A radiograph was used to find the bullet and showed an interesting bullet pathway. By observing the bullet pathway on a radiograph, sometimes we can surmise the victim's body posture at the time of an incident.

Firearms↗
PubMed1990

So-called branchiogenic carcinoma is actually cystic metastases in the neck from a tonsillar primary.

In 1974, we reported on six patients with squamous cell carcinoma arising in the tonsillar area who presented with a characteristic form of cystic nodal metastasis. This study reports on an additional 21 patients with this entity. Treatment of these patients included removal of the cystic metastasis with or without radical neck dissection and irradiation of Waldeyer's ring. Two patients have died of unrelated disease and two were lost to follow-up, but the remainder are alive without disease 2 months to 12 years following initial diagnosis. We believe that cystic metastases from tonsillar squamous cell carcinomas have often been mistaken for either primary squamous cell carcinomas of branchiogenic origin or with branchial cleft cysts. The distinctive histological features of cystic metastases reviewed after its correct identification can lead to the discovery of an unsuspected primary lesion and result in specific treatment options.

Adult↗
PubMed1990

Non-Hodgkin's lymphoma of the adrenal gland.

Primary non-Hodgkin's lymphoma of the adrenal gland was diagnosed in a 64-year-old woman. The histologic and immunohistologic features are characteristic of a large-cell noncleaved lymphoma (B-cell origin). The patient died 3 weeks after right-sided adrenalectomy. The patient's family refused an autopsy.

Adrenal Gland Neoplasms↗
PubMed1990

[Intrathoracic Pott's abscesses, radiologic pitfalls for the pneumologist].

The authors have conducted a retrospective study of 200 cases of tuberculous spondylitis, and wish to draw attention to the clinical and, chiefly, radiological aspects of the disease. Intrathoracic Pott's abscesses are principally observed in developing countries but they are not exceptional in France, and their varied semeiology deserves to be described.

Adolescent↗
PubMed1989

High-performance liquid chromatographic determination of pirprofen and five of its metabolites in human plasma without hydrolysis and in human urine before and after chemical hydrolysis.

Selective high-performance liquid chromatographic methods for the simultaneous determination of pirprofen and five of its metabolites either in plasma or in urine before and after chemical hydrolysis were developed. After addition of an internal standard and a buffer, the compounds were extracted from plasma using reversed-phase C18 Bond-Elut columns and from urine using pre-packed silica Extrelut 1 columns, back-extraction into sodium hydroxide and acidification of the alkaline phase before injection. Pirprofen, its five metabolites and the internal standard were separated using a linear elution gradient chromatographic system and wavelength programming. The analysis of spiked samples demonstrated the good accuracy and precision of the methods with limits of quantitation of 100 or 200 ng/ml for the different compounds in plasma, 200 or 360 ng/ml in urine without hydrolysis and 1 or 1.8 micrograms/ml in urine after chemical hydrolysis.

Chemical Phenomena↗
PubMed1989

Neural and glandular localisation of substance P in Echinostoma caproni (Trematoda-Digenea).

Immunocytochemical studies using antibodies against various peptides were performed on Echinostoma caproni. Only the presence of substance P or a related peptide was clearly demonstrated in this digenetic trematode. In the central nervous system, substance P-immunoreactive neurons were few and constituted two populations according to their size. Labelled axons were observed in the ventral and dorsal nerve cords. Scarce, small neurons and fibres were detected in the peripheral nervous system. The comparative study of 6- and 14-day-old worms showed an overall increase in substance P-immunoreactive nerve structures in the latter. Strong immunoreactivity for substance P was found in prostate cells from 14-day-old worms, whereas none was observed in 6-day-old worms. Thus, positive immunoreactivity seemed to be related to sexual maturity.

Animals↗
PubMed1989

Factors associated with the course and outcome of schizophrenia in India. Results of a two-year multicentre follow-up study.

The paper describes the two-year follow-up results from 323 out of 386 patients fulfilling a modified version of Feighner's criteria for diagnosis of schizophrenia, in Lucknow, Vellore, and Madras. There was remission in 66% of cases. Short duration of illness, consistent compliance with medication, positive attitudes of relatives and neighbours, absence of economic difficulties, increase in religious activities on the part of patients, a rural background, and a non-schizoid pre-morbid personality were associated with good outcome. Features of depression, dangerous behaviour, and absence of agitation were associated with poor outcome.

Adolescent↗
PubMed1989

[Contribution of the ethologic concept to the clinical approach].

Using an ethological approach in the context of Geriatric Psychiatry, an introductory attempt was made at the practical application to 5 cases of Alzheimer's disease, with an average evolution of 15 years. The analysis of several behavioural schemata can be of help to demonstrate the relevance of such an approach, and also to identify some of the most fruitful areas of investigation. Their study may help to enrich and modify current options to care.

Activities of Daily Living↗
PubMed1989

[Bronchial fistula caused by Pott's abscesses. Report of 3 cases].

The authors report 3 cases of intrabronchial fistulisation of paravertebral abscesses due to tuberculous spondylitis of the thoracic spine. This complication is relatively rare (these 3 cases were in a series of 178 cases of Pott's disease) and is easy to diagnose due to its characteristic radio-clinical features. As mentioned in old publications, this type of fistulisation does not seem to have serious consequences. There are no particular aspects of treatment.

Adult↗
PubMed1989

Factors influencing breastfeeding behaviour.

The influence of nine socio-demographic factors (family size; child spacing; socio-economic status; mother's employment, education, age and weight; birth weight; and father's education) on the duration of exclusive breastfeeding was studied in a group of urban mothers. Only a small fraction (15%) of the wide variability in breastfeeding behaviour could be related to these factors. Educated mothers alone started artificial feeds significantly earlier (p less than 0.05). Our findings suggest that the decision to breastfeed is largely an individual one and not related in any simple way to the easily measurable characteristics.

Breast Feeding↗
PubMed1988

Isoenzyme analysis of Echinostoma liei: comparison and hybridization with other African species.

Isoenzyme analysis was carried out on the laboratory strain of Echinostoma liei. The results were compared with those from a preliminary study on Echinostoma caproni, Echinostoma togoensis, and Echinostoma sp. (A. Voltz, J. Richard, B. Pesson, and J. Jourdane, 1986, Annales de Parasitologie Humaine et Comparée, 61, 617-623). Isoelectrofocusing showed characteristic phenotypes for phosphoglucomutase (PGM, EC 5.4.2.2) and glucosephosphate isomerase (GPI, EC 5.3.1.9). The four experimental strains were monomorphic. Their genotypes were defined. Isoenzyme analysis of F1-hybrids and their F2 descendants indicated the subunit structure of both isoenzymes and showed that they were encoded by independent genes. Finally, it also suggested that the four strains corresponded to variants of the same species.

Alleles↗
PubMed1988

Accuracy of rotavirus diagnosis: modified genome electrophoresis versus electron microscopy.

530 human faecal samples were examined for the presence of rotavirus by electron microscopy (EM) and by a modified electrophoretic analysis of viral genome (PAGE). 516 stools gave identical results by both methods (97.4% agreement). The proportion of EM+, PAGE- samples (1/530) was significantly lower than the proportion of EM-, PAGE + samples (13/530) (P less than 0.01 with McNemar test). Relative sensitivity was 99.6% for PAGE and 95% for EM. False positive results could be excluded for each method because of the characteristic morphology of rotavirus particles (EM) and specificity of rotavirus electrophoretypes (PAGE). Discrepant cases were reexamined whenever possible and the causes of misdiagnosis are discussed.

Electrophoresis, Polyacrylamide Gel↗