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Biomedical subjects

J Reina

Publications and source records attributed to J Reina.

At least 55 records · Page 3Linked to original sources
PubMed1996

Clinical and microbiological characteristics of Rahnella aquatilis strains isolated from children.

We report the microbiological characteristics of two Rahnella aquatilis strains isolated in the faeces of two patients with acute gastroenteritis, one of whom is an AIDS patient. The biochemical behaviour was studied with different automated identification systems, and the few clinical cases to be found in the literature were reviewed. Of the nine strains isolated in clinical samples, two were obtained from blood cultures, two from respiratory samples, one from urine, one from a burn wound, one from a surgical wound, and two (our strains) from faeces. In almost all cases the patient presented an underlying condition facilitating infection by opportunistic microorganisms. The majority of strains are characterized by their resistance to ampicillin, cephalothin and cefoxitin. Due to the rarity of the isolation of R. aquatilis in human samples it is not yet possible to establish, with any degree of certainty, its true pathogenic capacity.

Child, Preschool↗
PubMed1996

Evaluation of a direct immunofluorescence assay, dot-blot enzyme immunoassay, and shell vial culture in the diagnosis of lower respiratory tract infections caused by influenza A virus.

We prospectively evaluated the efficacy of two commercial rapid methods for antigenic detection, a dot-blot enzyme immunoassay (EIA-DB) (Directigen FluA, Becton-Dickinson, USA) and a direct immunofluorescence assay (DIF) (Monofluokit Influenza A, Diagnostics Pasteur, France), compared with the shell-vial culture in the MDCK line, incubated 2 to 3 days and stained with the monoclonal antibody clone IA-52, the diagnosis of lower respiratory tract caused by Influenza A virus (IA). In the study period the presence of IA virus was detected in 59 of the 377 samples analyzed (15.7%). Only the SVC method detected all positive samples (100% sensitivity), being used as a reference method for comparison with the other techniques). The EIA-DB technique detected 50 cases (84.7%) and the DIF only 35 (59.3%). In nine (15.2%) cases the diagnosis was obtained only with the SVC method. The results of the comparison of the EIA-DB technique with SVC were: sensitivity 84.7%, specificity 100%, positive predictive value 100%, and negative predictive value 97.2%. The DIF technique gave values of 59.3%, 100%, 100%, and 92.9%, respectively. A statistically significant difference was observed between the sensitivity of the EIA-DB and the DIF method (p = 0.0001). In view of the results we recommended the use of the EIA-DB as a screening method when infection by the IA is suspected. But to obtain the maximum diagnostic yield all samples would be inoculated in a shell vial culture with the MDCK cell line.

Bacteriological Techniques↗
PubMed1996

Correlation between human cytomegalovirus quantitative p72 antigenemia and viremia.

OBJECTIVE: We present a prospective study of the correlation between the human cytomegalovirus (HCMV) quantitative antigenemia with monoclonal antibody to p72 protein (immediate-early antigen) and the number of infected cell foci detected in the shell-vial culture. A comparative study was made of the value of quantitative antigenemia (pp65 and p72) in 14 patients. RESULTS: The average value of the pp65 antigenemia was 195 pp65-positive PMNLs per 10(5) PMNLs (range 10-1000) and that of the p72, 21 p72-positive PMNLs per 10(5) PMNLs (range 0-120) (P < 0.001). The p72 antigenemia value represented 10.7% of the pp65 value (range 4.4-70%). A statistical correlation was observed between the total number of infected cell foci detected in the shell-vial culture and the total number of p72-positive PMNLs (P < 0.001), but not with the number of pp65-positive PMNLs (P = 0.4). A study of the number of infected cell foci detected in the shell-vial per 100000 PMNLs inoculated showed a statistical correlation with the value of the p72 antigenemia (P < 0.001). CONCLUSIONS: According to results, there seems to be a general population of PMNLs carrying viral particles which are defected by means of the pp65 monoclonal antibody, and a subpopulation carrying active and replicative viral particles which is detected with the p72 antibody. This last subpopulation would be responsible for the formation of infected cell foci in the shell-vial culture. However due to the technical difficulties presented by the routine performance of p72 antigenemia, we recommend the routine application of the quantitative shell-vial culture and the use of the number of infected cell foci x 100000 PMNLs inoculated as a parameter of replicative viral load for the diagnosis of infection and disease caused by HCMV.

Antibodies, Monoclonal↗
PubMed1996

Gastroenteritis caused by Aeromonas trota in a child.

A case of acute diarrhoea caused by Aeromonas trota (formerly HG 13 group) in a Spanish child is reported. The strain was isolated in the faeces using the CIN agar (cefsulodin-irgasan-novobiocin) culture media. The strain was initially identified as A sobria by the commercial GNI card and API 20E biochemical systems. The strain was, however, VogesProskauer and sucrose negative, so complementary tests of cellobiose fermentation and gluconate oxidation were performed. These tests, together with the strain susceptibility to ampicillin (MIC 1 microgram/ml) and carbenicillin (MIC < 16 micrograms/ml) led to the final identification of A trota. The microbiological characteristics of this new species and the principal tests required for its identification are presented. The isolation, for the first time, of A trota in the Mediterranean area confirms the suspected worldwide distribution of this species.

Acute Disease↗
PubMed1995

Evaluation of the API-campy system in the biochemical identification of hippurate negative campylobacter strains isolated from faeces.

The aim was to evaluate the efficacy of the API-Campy system in the biochemical identification of 62 hippurate negative campylobacter strains isolated from the faeces. The strains were identified manually as 34 nalidixic acid susceptible C coli (NAS), 20 nalidixic acid resistant C coli (NAR), and eight C lari. The 34 strains of NAS C coli were identified as such by the API-Campy system. Of the 20 strains of NAR C coli, 15 (75%) were correctly identified by the commercial system. None of the five NAR C coli strains which were also erythromycin resistant was identified as such by the system. The eight C lari strains could not be identified by the API-Campy system because the bionumber obtained does not exist in the database of the computer system. The API-Campy system could be very useful for the identification of NAS C coli. However, failure to allow for a higher percentage of resistance to nalidixic acid in this species does not permit good identification of NAR strains. More important discrepancies are observed in C lari strains. In order to improve the identification of NAR C coli and C lari stains, it is advisable to include, or recommend as complementary, the indoxyl acetate hydrolysis test.

Bacterial Typing Techniques↗
PubMed1995

[Presence of virulence factors and pathovars in strains of Campylobacter jejuni isolated from patients with diarrhea and healthy children].

BACKGROUND: To study the prevalence of virulence factors (adhesion, invasion, cytotoxicity and hemolytic activity) and to establish the presence of pathovars (virulence phenotype) in C. jejuni strains isolated in pediatric patients with inflammatory and secretory diarrhea and asymptomatic carriers. METHODS: We analyzed 95 strains of 48 patients with inflammatory diarrhea (blood and mucus in feces), 30 patients with secretory diarrhea (watery) and 17 strains isolated in asymptomatic children (control group). The study of adherence capacity, invasion and cytotoxicity was made in the Hep-2 cell line, and the analysis of hemolytic activity in blood agar plates with a 5% sheep's blood. The pathovars were defined by the cellular adhesion (phenotypes A and a) and the cytotoxicity (phenotypes E and e). RESULTS: 29.1% of inflammatory strains presented adherence capacity, 66.6% were invasive, 64.5% cytotoxic and 52.1% hemolytic. In the secretory strains the values were 70, 20, 10 and 6.6% respectively; in the control group the 11.7% presented adherence capacity and 5.8% were invasive. We obtained difference statistically significative for the secretory strains in the adherence capacity, and in inflammatory strains in the adherence capacity, cytotoxicity and hemolysis. The phenotype Ae predominate in the secretory strains, and the phenotype ae in the strains belonging to the control group. No pathovar predominates in the inflammatory strains. CONCLUSIONS: The analysis of virulence markers permit us to establish the pathogenic behaviour of the C. jejuni strains isolated in patients with diarrhea. The study of the adherence capacity and cytotoxicity (pathovars) would be used as a virulence markers and to predict the inflammatory or secretory nature of the diarrhea caused by C. jejuni strains.

Bacterial Adhesion↗
PubMed1994

Susceptibilities to 10 antimicrobial agents of 1,220 Campylobacter strains isolated from 1987 to 1993 from feces of pediatric patients.

We report the in vitro antibiotic susceptibility of 1,220 strains belonging to the thermotolerant Campylobacter species, isolated from the feces of pediatric patients with diarrhea in the period from 1987 to 1993. The strains were identified as 1,148 C. jejuni isolates and 72 C. coli isolates. The overall results show that the strains showed drug resistance as follows: 51.8% to ampicillin, 4.4% to clindamycin, 2.6% to chloramphenicol, 21.2% to tetracycline, and 1% to gentamicin. Twenty-one strains (1.7%) displayed resistance to the combination of amoxicillin-clavulanic acid, and 3.2% of the strains were resistant to erythromycin (MIC of > or = 4 micrograms/ml), with a notable difference according to the species under consideration. While C. jejuni remained stable at 0.9 to 4% resistance to erythromycin, for C. coli the percentages detected ranged from 0 to 33%, with overall rates of 2.5 and 15.2% for the two species, respectively. Resistance to nalidixic acid (MIC of > or = 32 micrograms/ml) was found in 27.2% of the strains (27.8% for C. jejuni and 18% for C. coli), and resistance to ciprofloxacin (MIC of > or = 4 micrograms/ml) was found in 24.2% of the strains for C. jejuni and 15.2% for C. coli). Cross-resistance between nalidixic acid and ciprofloxacin was found in 89.1% of the strains (type 1 mutants), while 10.9% were resistant to nalidixic acid but susceptible to ciprofloxacin (type 2 mutants).

Anti-Bacterial Agents↗