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Biomedical subjects

J Potel

Publications and source records attributed to J Potel.

At least 55 records · Page 3Linked to original sources
PubMed1982

[In vitro testing of yeast resistance to antimycotic substances].

Investigations have been carried out in order to clarify the antibiotic susceptibility determination of yeasts. 291 yeast strains of different species were tested for sensitivity to 7 antimycotics: amphotericin B, flucytosin, nystatin, pimaricin, clotrimazol, econazol and miconazol. Additionally to the evaluation of inhibition zone diameters and MIC-values the influence of pH was examined. 1. The dependence of inhibition zone diameters upon pH-values varies due to the antimycotic tested. For standardizing purposes the pH 6.0 is proposed; moreover, further experimental parameters, such as nutrient composition, agar depth, cell density, incubation time and -temperature, have to be normed. 2. The relation between inhibition zone size and logarythmic MIC does not fit a linear regression analysis when all species are considered together. Therefore regression functions have to be calculated selecting the individual species. In case of the antimycotics amphotericin B, nystatin and pimaricin the low scattering of the MIC-values does not allow regression analysis. 3. A quantitative susceptibility determination of yeasts--particularly to the fungistatical substances with systemic applicability, flucytosin and miconazol, -- is advocated by the results of the MIC-tests.

Antifungal Agents↗
PubMed1981

Killing of bacteria with electric pulses of high field strength.

Bacteria of the type E. coli K12 have been treated in experiments using high-voltage pulses of short time (microseconds) as a killing agent. The role of different experimental parameters has been studied: kind of electrolyte, concentration, length of pulses, field strength, pH and temperature. Electrolytes with bivalent cations were found to reduce the lethal action. the relative rate of killed bacteria was shown to be mainly governed by the field strength and the treatment time, which is defined by the product of pulse number and decay time constant. From the obtained results a function has been developed which enables the precalculation of the killing rate for E. coli, provided that certain limits of experimental conditions are considered. No correlation between the applied electric energy and the lethal effect could be found.

Bacterial Physiological Phenomena↗
PubMed1979

[Mezlocillin: tissue concentration and efficacy (author's transl)].

Studies on serum and tissue (cutis, muscle and liver) concentrations were carried out with mezlocillin (Baypen), a semi-synthetic new acyl ureidopenicillin, in 51 patients. Serum half life was 59 minutes. The highest concentrations were measured in the cutis, the lowest in the muscles. Concentrations in the liver were found to be relatively constant and persistent.--The efficacy and tolerance of mezlocillin were examined in 48 patients affected by 56 mostly severe infections, which were treated with a dose of 5 g twice or three times daily. The indications for antibiotic treatment were bronchopulmonary infections, peritonitis, soft tissue infections, biliary and urinary tract infections and postoperative febrile conditions of unknown origin. Clinical cure was achieved in 78.5% and an improvement of symptoms in 5.4% of infections. 76% of the causative microorganisms were eliminated. Mezlocillin was locally and systemically well tolerated.

Drug Evaluation↗
PubMed1979

[On the serology and immunbiology of listeriosis. VII. Communication: Further investigations on the agglutination-immobilization test (author's transl)].

Factor antisera oriented to different serovars of Listeria moncytogenes, expecially to serovar 7 and Murray grayi (Listeria grayi) were tested. It was found that the agglutination-immobilization test detects antibodies oriented to the H-antigen. By contrast, the growth inhibition test measures antibodies oriented to the O-antigen. Summarizingly, it can be stated that the following three seroreactions, each based on a different mode of action, are available for the determination of antibodies specifically oriented to the somatic or flagellar antigens of L. monocytogenes: 1. Bacterial agglutination reaction for the detection of O and H antibodies 2. The growth-inhibition test for the detection of O antibodies 3. The agglutination-immobilization test for the detection of H antibodies.

Animals↗
PubMed1978

[Hospital infections with pseudomonas aeruginosa. II. Intensive care units (author's transl)].

72 newly admitted patients of three surgical intensive care units of the Medical School Hannover were examined bacteriologically for pseudomonas aeruginosa for a period of 7 months. A total of 810 specimens was examined during therapy. 95 strains of pseudomonas aeruginosa were isolated in 32 of 72 patients, taken either at the time of admission or during the stay of the patients in the hospital. The frequency of contamination increased with the duration of the stay of the patients in the hospital as follows: 20 per cent at the time of admission, 71 per cent after 8 to 10-day stay and up to 100 per cent for a duration of stay exceeding 14 days. The germs were mainly localized in the nose-throat region and in the respiratory tract. The results of phage typing suggested a hospital infection in about 50 per cent of the patients. The relationship between infection and infectious disease was discussed with respect to the epidemiologic characteristics of intensive care units. Furthermore, it was attempted to formulate recommendations for interrupting or abolishing the infection chains.

Cross Infection↗
PubMed1977

[Hospital infections with pseudomonas aeruginosa: I. Technical devices and wet areas as sources of infection in intensive care units (author's transl)].

In an intensive care unit system comprising three surgical intensive care wards bacteriologic investigations on the presence of Pseudomonas aeruginosa were carried out over a period of 7 months (as an example for the aetiology of hospital infections). Centers of contaminations were found to exist in wet areas and in technical devices (ultrasonic nebulizers, respirators etc.). In many cases, the bacterial strains isolated from patients were found by means of phage-typing to be identical with strains isolated from devices and wet areas. Additional experimental investigations showed the predominant role of ultrasonic nebulizers for germ dissemination. On the basis of the results obtained a patient oriented scheme of probable infection chains was tried to establish. Hereby the wet areas play a central role as reservoirs of germs and therapeutical devices as vectors. The results obtained were evaluated in order to define a catalogue of practical measures for interruption of infection chains.

Bacteriophage Typing↗