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Biomedical subjects

J Planas

Publications and source records attributed to J Planas.

At least 73 records · Page 4Linked to original sources

Plasma variation of transferrin-iron and phosvitin-iron during the laying period in chicken hens.

The distribution of plasma iron between transferrin-iron (Tf-Fe) and phosvitin-iron (Phv-Fe) over a 98 week laying period was established in hens. Six plasma samples were collected at random from a group of 20 pullets, starting when the birds were 20 weeks old. Samples were collected weekly until the 29th week and subsequently on the 41st, 62nd, and 98th weeks. Detailed assays were carried out to differentiate Phv-Fe from Tf-Fe. Phosphoprotein (phosvitin) concentrations were also determined. At the beginning of sexual activity, (23 to 24 weeks) females showed a parallel increase of Tf-Fe and Phv-Fe. During the period of maximum egg production 29 to 41 weeks) Tf-Fe was 65% and the Phv-Fe 35% of the total plasma iron. During this period the transferrin saturation reached a maximum of 80%; both transport mechanisms worked simultaneously. Estrogen administration to male chickens (2 injections of 5 mg/kg body weight) precipitated similar variation patterns of the two plasma irons over a period of 7 days. It is suggested that the estrogens were responsible for these variations.

Animals↗

Erythrocyte size in some wild Spanish birds.

An analysis was made of the erythrocyte dimensions of 21 species of the following Passeriformes common to the Iberian Peninsula: Fringilidae, Silvidae, Turdidae, Prunellidae, Ploceidae, Certhiidae, Emberizidae, Paridae, Troglodytidae and Apodiformes: F. Apodidae. The erythrocyte dimensions in Passeriformes were very uniform. Only the Silvidae presented a significant correlation (r = 0.96) between body size and cell area. In Apodiformes, cell area and cell length and width were significantly greater (p less than 0.01) than red blood cell dimensions in Passeriformes.

Animals↗

Iron absorption in the everted chicken intestine.

Everted chicken intestines from 2-4 week old males were prepared according to the Wilson and Wiseman technique. The sacs were kept in a glucose ionic balanced solution, containing different cold iron concentrations (0.02; 0.01; 0.5; 1 mM Fe) and 59Fe was added as a tracer. The mucosal iron uptake and the iron transfer in the serosal fluid were determined at different intervals (1; 5; 10; 20 min) and in three main segments. The viability of the intestinal sacs was controlled by manometric O2 evaluation. The iron mucosal uptake was proportional to the iron concentration but the serosal iron transfer presented a saturation level, with similar behavior for both the duodenum and the jejunum. Iron transfer decreased with time and this could suggest a serosal to mucosal reflux. No effect was noted from the presence of metabolic inhibitors (0.01 mM sodium azide or 0.5-2 mM 2-4-dinitrophenol).

Animals↗

Iron mobilization and plasma ferroxidase factors in chickens.

Hematological values, metal content (Fe, Cu, Zn, Cd) in plasma and liver, ceruloplasmin (p-phenylendiamine oxidase) and ferroxidase activity in plasma, were analyzed in chicks, fed during 10 weeks on a commercial diet, supplemented by either 5,000 ppm Zn or 100 ppm Cd. A microcytic and hypochromic anemia was evident in both groups but in the Cd-fed chicks, plasma iron and ceruloplasmin values were normal. Estrogen administration mobilized iron in the Cd-group but not in the Zn-group. The precipitation of plasma phosvitin reduced (90%) the ferroxidase activity that had been previously induced by the estrogens. Correcting copper levels in the Zn-group, by copper injection, restored the ceruloplasmin level. However, the estrogens, in such birds, neither mobilized the plasma iron nor increased the ferroxidase activity. Plasma citrate was determined in laying, non-laying hens and in estrogenized or normal males. The contribution of citrate to the ferroxidase activity of plasma during the laying period, was negligible. It was concluded that plasma phosvitin during laying was the main factor responsible for the ferroxidase activity. However, the ceruloplasmin in chickens, could play a secondary role in iron mobilization.

Anemia, Hypochromic↗

Improving breast shape and symmetry in reduction mammaplasty.

In any bilateral operation, symmetry is of primary importance. When the shape of the remodeled breast is unsatisfactory after excess tissue has been removed, the surgeon may feel that leaving more tissue as support would improve the shape of the breast. When this happens in the second breast, there is not only an unsatisfactory form but asymmetry as well. This paper describes our organization of the steps in the operation, and underscores the importance of Gillies' maxim, "In plastic surgery, never throw anything away."

Adult↗

Iron metabolism in pigeons.

Several haematological parameters such as haemoglobin concentration, haematocrit, erythrocyte number, reticulocyte concentration, plasma iron and total iron binding capacity were determined in 118 urban pigeons of both sexes. No statistically significant sex differences among these parameters were found. In 36 specimens (23 males and 13 females), the plasma iron turnover was determined using 59Fe. The results obtained in this species, expressed per 100 ml-1 blood. day-1 and Kg-1 body weight. day-1, were compared with those of turkeys, ducks and chickens calculated from earlier papers. The highest values versus body weight were observed in pigeons. Organ (liver, spleen, tibia, heart, leg muscle, ribs, sternal keel, gonads and blood) distribution of 59Fe intravenous injection was analyzed during a period from 5 min up to 120 days (19 different times) in groups of 4 pigeons. At the 6 h period, the organs retained the highest dose (20% of total Fe injected), but by the 2nd day period, the radioiron in the blood represented about 98% of the total injected. A fast iron uptake by the circulatory blood was checked and compared with that of other species (domestic fowl, ducks and turkeys). The reticulocyte count in pigeons normally ranged from 4 to 12%, which was consistent with these results. A linear decreasing radioactivity in blood, with an inflexion point on the 40th day was observed. An inverse correspondence between blood and liver was found. Content in other organs decreased uniformly with time, except the heart where the iron content was practically constant during the whole time. Ribs and sternal keel are erythropoietic organs in young pigeons.

Animals↗

Haptoglobins in some Galliformes birds.

In several groups of galliform birds (chicken, turkey, pheasant and guinea fowl) the presence and function of plasma haptoglobin (Hp) have been studied. Paper, acetate and starch-gel electrophoresis were carried out and two benzidine stain regions were observed when haemoglobin (Hb) was added to normal plasma (Hb-Hp complex and methemalbumin) besides the free Hb when the binding capacity of these proteins were surpassed. The disappearance of the administered Hb-59Fe was exponential which is evidence of the existence of two different rates. Liver, bone marrow and kidney are the main organs related to the 59Fe uptake. These results on Hp in galliforms and the metabolism of the Hb are concordant with the data in the literature on mammals. This suggests the same common physiological role for Hp.

Animals↗

The twisted nose.

To the patient seeking correction of a twisted nose, it is as important to obtain a good shape as it is to achieve normal breathing. The functional problem must be solved regardless of the extent of the resection required. The plastic surgeon may choose among many techniques, some of which have been elucidated. Our preferred approach has been described in detail. We reiterate that experience has shown that a deviated septum which requires stabilization after surgery will recur to a greater or lesser degree at a later date. A twisted nose, to be permanently corrected, should maintain its corrected position without external support at the end of the operation.

Anesthesia, Local↗

Introduction of breast implants through the abdominal route.

We present our technique of doing an abdominal lipectomy and augmentation mammaplasty in the same operation, through the same incision, in selected patients. We introduce the breast implants through tunnels made from the top of the abdominal pocket into the breast areas. We have had no complications in 12 patients in whom we have done this operation.

Abdominal Muscles↗

Mammary augmentation--surgical techniques, evaluation of results, and complications.

Mammary augmentation is the procedure of choice in all forms of breast underdevelopment, atrophy or absence due to disease (subcutaneous or radical mastectomy), or trauma (burns of the thoracic region). Old reconstructive methods employing autogenous tissues have no substitute in special cases, but they are used less and less in favor of the new alloplastic materials (silicones) introduced as implants under the mammary gland. These implants have been shown to be permanently accepted by body tissues, the aesthetic results are good, and the operation is easier, faster, and less dangerous. Complications, however, do exist and the most frequent is the constriction of the fibrous envelope surrounding the implant. The etiology of this complication is still unknown. The patient should be advised of such a possibility. Circular incision of the fibrous envelope seems to be the best treatment for this complication. Silicone implants do not modify the physiology of the mammary gland and there is no reason to believe they have any influence on the incidence of breast cancer.

Adipose Tissue↗

The serum ferroxidase system and the effect of estrogen on plasma iron.

Estrogen effects on plasma iron and ferroxidase activity in some mammals and domestic fowl are studied, to investigate a possible estrogen mechanism on iron through its action on the ferroxidase system. Although estrogen generally induces ceruloplasmin, iron mobilization, characterized by a rise in plasma iron, was evident only in rats and chickens. Gonadotrophin treatment confirmed these results. A decreasing affect on plasma iron was noted in rabbits and guinea-pigs, substantiated by some bibliographical data. Ferroxidase activity increased and a copper-dependent factor was evident in copper injected species. Iron mobilization, however, was produced only in rats and chickens. D-penicillamine treatment considerably lowered ferroxidase activity in rats and suppressed the estradiol increasing plasma iron effect. This response to the copper-chelating drug did not take place in the other species. This phenomenon could be explained by the presence of two copper-dependent ferroxidases (ferroxidase I or ceruloplasmin and ferroxidase II) in rat plasma, as recently published.

Animals↗