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Biomedical subjects

J Planas

Publications and source records attributed to J Planas.

At least 55 records · Page 3Linked to original sources

Further advantages of the "vest over pants" abdominoplasty.

The standard abdominal lipectomy is a risky procedure with respect to where the first incision (i.e., the transverse suprapubic incision) is located. Failure to place the incision successfully may distort the whole procedure, resulting in an overly tight abdominal wall, hypertrophy, and upward displacement of the scar along with the pubic hair, and also excessive tension causing skin necrosis of the abdominal flap. Advantages of our reversed technique are emphasized.

Abdominal Muscles↗

Seasonal variations of insulin and some metabolites in dogfish plasma, Scyliorhinus canicula, L.

Plasma levels in insulin, glucose, ketone bodies, and lactate were analyzed during a 1-year period in the lesser spotted dogfish (Scyliorhinus canicula) in captivity. Plasma insulin levels fluctuated similarly for both sexes. The highest insulin levels were found during late prespawning (from January to March) and another increase was observed during the active feeding period (September and October). During the spawning period, insulin decreased and in the postspawning period the lowest values were reached. Glucose, however, showed a different trend. It was at a minimum in late prespawning and it reached its highest values in postspawning. It decreased again in September and October. Plasma ketone body levels were highest in late prespawning and could be considered as an alternative energy source for this hypoglycemic period. In the summer months plasma lactate levels rose, with maximum levels occurring in July.

3-Hydroxybutyric Acid↗

The effect of temperature on immunoreactive glucagon plasma level in carp Cyprinus carpio.

Plasma immunoreactive glucagon levels (IRG), plasma glucose levels and brain and liver glycogen concentrations were analyzed in carp (adapted to 15 degrees C) subjected to short-term temperature changes (1.6 or 11 h, at 5 degrees C or 28 degrees C, respectively) and to long-term temperature changes (21 months at 28 degrees C). The high temperature (28 degrees C) produced significant increases in IRG in both short and long-term experiments. Brain glycogen also decreased in both experiments whereas liver glycogen only changed in the long-term experiment. Low temperatures did not provoke any changes either in IRG or in liver glycogen, whereas brain glycogen decreased in the 1 h exposure. In short, under these conditions in carp, IRG did not respond to low temperature but could play an important role in high temperature acclimation.

Animals↗

Iron metabolism in iron-deficient male quail.

1. Male quails submitted 20 and 120 days to a low iron diet (7 ppm) were compared to female laying quails, exposed for 30 days to the same low iron regime, in order to compare the response of the iron metabolic control under a single (erythropoiesis) or a doubled (erythropoiesis and egg formation) iron demand. 2. Iron deposit in storage organs, the classical hematology and the intestinal iron absorption were analyzed in these animals. 3. In males, after 120 days, the iron deposits were reduced 50 and 75%, but hematological values (hematocrit and hemoglobin concentration) were normal, although in laying quails, after 30 days, an anemic condition was evident in both blood parameters and iron deposits, provoking an iron deficient erythropoiesis. 4. The enhancement of the intestinal iron uptake, confirms the anemic character of these birds.

Animals↗

Iron content and distribution in Japanese quail.

Determination of blood haematocrit and haemoglobin, plasma iron content, total iron binding capacity and phosphoprotein (vitellogenin), and the iron content of different organs (pectoral muscle, liver, spleen, intestine, femur bone marrow, blood, gonad and eggs, and feathers) were carried out in prelaying, onset of laying and full laying females, as well as in adult males. The whole iron content was also determined on incinerated specimens giving these results: 53.7 parts per million (p.p.m.) iron in the prelaying group, 54.5 p.p.m. iron at the onset of laying, 64.3 p.p.m. iron in full laying and 53.5 p.p.m. iron in males. The plumage represented 5-8% of the total body weight, and its iron content oscillated between 152-163 p.p.m. iron in males and non-laying females and 177.3 p.p.m. iron at full laying. The laying period induced important variations in plasma levels and in organ distribution, but not in haematological values. The first eggs laid were smaller (9.2 g) and richer in iron (427 micrograms iron) than those laid by older layers (11.8 g and 305 micrograms iron). The percentage distribution of the total iron content of organs was, in prelaying females: feathers, 21.9; blood, 56.6; pectoral muscle 8.1; liver, 9.7; intestine, 2.7. In laying females: 29.1; 46.6; 11.5; 7.4; 4.2. In males: 17.4; 59.6; 11.4; 7.9; 2.3. The increase in intestinal iron content in laying quails coincided with a double intake of food. This distribution differs from the mammalian model, as egg production (where each egg represented 2.5-5.5% of the total iron) and the great inert iron deposits in the plumage require an elaborate iron metabolism control system to cover all the iron needs in birds.

Animals↗

Pentachlorophenol and hexachlorobenzene in serum and urine of the population of Barcelona.

1 Urinary chlorophenols of the general population of Barcelona, Spain were determined. Pentachlorophenol (PCP: 25.0 +/- 3.9 ng/ml; mean +/- s.e.m., n = 50) and tetrachlorophenol (TCP: 6.2 +/- 1.6 ng/ml; mean +/- s.e.m., n = 25) were found in all samples. 2 Pentachlorophenol and hexachlorobenzene were also determined in serum. Both were present in all samples (PCP: 21.9 +/- 1.9 ng/ml; HCB: 11.1 +/- 1.1 ng/ml; mean +/- s.e.m., n = 100). Their concentrations do not show any correlation, suggesting no metabolic relation between them.

Chlorobenzenes↗

Plasma glucagon levels in different species of fish.

Plasma glucagon levels in 11 different teleosts and in dogfish were evaluated using an adapted classical mammalian radioimmunoassay (RIA). The values obtained are considered relative; sensitivity was inferior to the original method, but the validation experiment results are acceptable. The mean value of the coefficient of the variation for interassay was 6% and for intraassay 4%, recovery was 98%, and a good linearity in the dilution test was found. Some species (Sparus aurata and cyprinids) presented high plasma glucagon concentrations (0.50-1.60 ng/ml) versus the lower levels (0.08-0.40 ng/ml) found in salmonids and others, and the lowest in dogfish (0.02-0.20 ng/ml). A positive correlation (P less than 0.02) between insulin and glucagon plasma levels was found in different species. The low levels obtained in all parameters analyzed in the selachian were remarkable. This adapted RIA could serve as a useful tool in amplifying knowledge on the endocrine pancreatic response in fish in different biological situations.

Animals↗

Effects of confinement stress and additional zinc treatment on some blood parameters in the dogfish Scyliorhinus canicula.

Several blood parameters were studied in the dogfish Scyliorhinus canicula subjected to different confinement conditions (transport and maintenance) and zinc contamination. Confinement stress significantly decreased erythrocyte counts (RBCC), haematocrit (Ht), haemoglobin (Hb), leucocrit (Lt), mean corpuscular haemoglobin (MCH) and mean corpuscular haemoglobin concentration (MCHC). Mean corpuscular volume (MCV) did not change with this stress and plasma glucose concentration significantly increased. In fish subjected to confinement stress conditions, Zn treatment significantly decreased Hb, MCH and MCHC and plasma glucose concentration. Ht and MCV did not change and Lt and RBCC significantly increased. Results are discussed related to other stress effects and effects of zinc treatment on fish confined in non-stressful conditions.

Animals↗

Influence of laying on iron metabolism in quail.

Haematological and iron content values for liver, spleen, intestines, blood, ovary and eggs were determined in female quails, from the prelaying state to full laying. A drop in hematocrit, haemoglobin and in the liver stores occurred at the onset of laying (48 to 51 d of age), but these values recovered after 5 to 10 d. The iron lost in eggs (0.3 mg Fe/egg) was responsible for this. An active homeostatic control must be necessary to supply adequate iron for both eggs and blood formation. Quails fed on an iron-deficient diet distributed iron preferentially to the haematopoietic processes, at the expense of the iron content of the eggs and a subsequent reduction of viability.

Animal Nutritional Physiological Phenomena↗

Gill metabolism after chronic subacute zinc treatments.

ATP and lactate concentration of the gill tissue of Scyliorhinus canicula were determined after short-term and long-term subacute zinc exposure. Increase of lactate and decrease of ATP levels were detected after short-term exposure, while no significant changes were observed after long-term treatment, suggesting a recovery process or an activation of compensatory mechanisms.

Adenosine Triphosphate↗

Daily rhythms of insulin and glucose levels in the plasma of sea bass Dicentrarchus labrax after experimental feeding.

Significant and inverse circadian rhythms are demonstrated in glucose and plasma insulin in fish fed a natural diet. The highest glucose levels are found during the light period, around feeding time, and the insulin level peaks during the dark period. As a possible cause for the insulin rhythmicity, the daily variations in several plasma amino acids are considered. The feeding times could be a training factor for metabolic rhythms, which are maintained even during a fast of 7 days. The differences in the compositions of the diets could be responsible for the lack of circadian rhythms in fish fed on a commercial diet.

Animals↗

Iron mobilization in estrogenized male quail.

A single diethylstilbestrol (DES) injection (5 mg DES/100 g body wt) was administrated to several lots (three specimens each) of adult male quail (Coturnix coturnix japonica). The birds were sacrificed 24, 48, 72 and 96 hr after the DES injection. A significant increase in liver weight and a clear drop in the hemoglobin concentration were observed after 24 hr. Later, a progressive rise was observed in plasma iron, total iron binding capacity, plasma copper and the phosphoprotein (vitellogenin), which reached highest values after 96 hr. In the liver, the iron showed an initial increase (24 hr), due to a rise in non-ferritin iron followed by a progressive decrease. Ferritin iron increased slowly but was significantly higher after 96 hr. This experimental model on male quail suggests an estrogen response in birds that could be more general and uniform than in mammals.

Animals↗

The use of a buried wire frame to reduce contracture of grafts.

Contracture following the successful take of a split-thickness cutaneous graft may be difficult to avoid if a force opposite to the contraction cannot be applied. In this paper, a procedure is described in which a wire-frame buried around the graft maintains it expanded during the contractile period.

Adolescent↗

Erythrocytic phosphates and flying activity in birds.

1. The intraerythrocitary organic phosphates in different avian species (Sturnus vulgaris, Apus apus, Gallus gallus domesticus, Coturnix coturnix japonica and Columba livia) were analyzed by ionic exchange chromatography. The chromatograms of these species revealed the presence of ADP, ATP, GTP, IPP and inorganic phosphate. 2. The main organic phosphates (ATP and IPP) and the inorganic phosphate were also evaluated, directly, in other specimens. The IPP levels were very similar in all species; however, for ATP, there were great differences, with the lowest value in chickens (1.53 mumol/ml) and the highest value in starlings (5.58 mumol/ml). 3. A progressive training program for homing pigeons with flights from 80 to 760 km did not cause significant variations in IPP levels. Nevertheless, the ATP concentration values showed significant changes. 4. A specific relationship between the flying habits in different species of birds or the training exercise for pigeons and the erythrocyte organic phosphates, was not evident. 5. Hematocrit and hemoglobin values showed significant changes during the progressive training program.

Animals↗