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Biomedical subjects

J Peters

Publications and source records attributed to J Peters.

At least 541 records · Page 30Linked to original sources
PubMed1985

[Method for contaminating test objects with coagulated blood].

A special method to contaminate test objects with blood is described. It is characterized by the use of coagulable blood and its coagulation when adhering to the test objects. Above all, the method is suitable for the microbiological evaluation of the efficacy of agents and methods for the disinfection of surfaces to which coagulated blood will adhere (e.g. instruments). Immediately upon sampling, the blood is heparinized (Liquemin 500 from Hoffmann-La Roche AG at a ratio of 0.1 ml per 100 ml blood). Until use, the coagulable blood is stored at 0 degrees C. For the contamination of test objects, the test organisms are added to the blood (at 0 degrees C), either by mixing the blood with a small amount of a suspension of the test organism or by centrifuging this suspension and dispersing the sedimented organisms in the blood. To start coagulation, Protamin 1000 (Hoffmann-La Roche) is added at a ratio of 0.15 ml per 10 ml blood. Immediately afterwards, the test objects are contaminated with the coagulating blood. Until complete coagulation of the blood, taking ca. 15 min, the test objects are stored in a humid chamber at ca. 20 degrees C.

Blood Coagulation↗
PubMed1985

Amebiasis presenting as pleuropulmonary disease.

Seven patients with amebic liver abscess presenting as pleuropulmonary disease were admitted to hospital initially because of pulmonary symptoms and were found to have amebic liver disease. Three categories of pleuropulmonary involvement included reactive inflammation of the pleura or lung, rupture of a hepatic abscess into the pleural space and rupture of a hepatic abscess into the bronchial airways. The preferred medical treatment is with metronidazole, but rupture of hepatic amebic abscess into the pleural space requires drainage in addition to medical therapy. In contrast, rupture into the bronchus may provide spontaneous drainage so that only medical therapy is needed. Recovery from amebiasis in all three categories is generally complete. Morbidity and mortality increase with failure to correctly identify amebic infection of the liver as the underlying cause. Because, in new cases, no findings specifically suggest that pleuropulmonary disease is a complication of hepatic amebic abscess, this possibility needs to be considered, especially in persons who are at risk of having been infected with amebae.

Adult↗
PubMed1985

[Ileocecal invagination--an unequivocal computer tomography diagnosis].

The diagnosis "ileocaecal invagination caused by lipoma" is possible by CT alone. The intraluminal lipoma can be identified via density measurement. The invaginated ileum causes an intraluminal mass caudal to the lipoma. Intussuscipiens and intussusceptum are incompletely separated by a crescent of mesenteric fat.

Adult↗
PubMed1984

Conservative management of pregnancy in diabetic women.

In 1979 the obstetric management of pregnancies in diabetic women in Cardiff was changed from elective delivery at 37-38 weeks to delivery at term. This change was facilitated by home monitoring of blood glucose concentrations and improved techniques for assessing fetal wellbeing. There were 35 pregnancies in insulin dependent diabetics in 1972-8 and 45 in 1979-82. The quality of diabetic control during pregnancy was equally good in both periods. The average gestation at final admission to hospital increased from 30 to 37 weeks. Amniocentesis to assess fetal pulmonary maturity was necessary in 26 patients (74%) in the first period of study and in only four (9%) in the second. Gestational age at delivery increased from 37.4 to 39.4 weeks after the change in policy. The proportion of mothers entering spontaneous term labour and delivering vaginally increased from 14.3% to 37.8%. The mean birth weight of live born, singleton infants increased from 3090 g to 3650 g, the feeding pattern improved, and respiratory problems were less common. Morbidity was reduced and perinatal mortality was not increased with conservative management of pregnancy in diabetic women.

Apgar Score↗
PubMed1984

The Pk-3 gene determines both the heart, M1, and the kidney, M2, pyruvate kinase isozymes in the mouse; and a simple electrophoretic method for separating phosphoglucomutase-3.

We have found that in mice carrying Pk-3r, an allele leading to loss or activity of kidney pyruvate kinase, the activity of heart pyruvate kinase is also diminished. Electrophoretic studies on tissues from mice carrying Pk-3r and/or Pk-3b, an allele determining an electrophoretically detectable variant, show that Pk-3 affects the expression of both the heart, M1, and the kidney, M2, pyruvate kinase isozymes. These results, together with linkage data, indicate that both isozymes are determined by the same structural gene, Pk-3. We also report a simple method for separating phosphoglucomutase-3 (PGM-3) by electrophoresis on cellulose acetate plates.

Animals↗
PubMed1984

Whole body oxygen consumption in awake, sleeping, and anesthetized dogs.

To study the metabolic effects of anesthesia, whole-body oxygen consumption (VO2) was compared on 242 occasions in six dogs under standard conditions while awake, sleeping, or anesthetized. The dogs were trained to lie unrestrained in the lateral position for the measurement of VO2 (STPD) in the unanesthetized state. Arterial blood gas tensions, pHa, heart rate, and blood pressure also were determined. The maximum VO2 of the alert resting and the minimum of the drowsy resting state averaged (+/- SE) 5.57 +/- 0.48 and 3.97 +/- 0.41 ml X kg-1 X min-1, respectively. VO2 was lowest and least fluctuating during natural sleep (2.46 +/- 0.2 ml X kg-1 X min-1). During deep anesthesia with methohexital, thiopental, and etomidate, VO2 averaged (+/- SE) 4.68 +/- 0.26, 4.26 +/- 0.28, and 4.77 +/- 0.35 ml X kg-1 X min-1 during spontaneous ventilation (open-circuit flow-through technique) and 3.54 +/- 0.27 ml X kg-1 X min-1 during controlled ventilation (open-circuit collection technique) with 2% halothane. Anesthesia reduces VO2 relative to the resting values of the alert state but increases it relative to that of natural sleep. Accordingly, anesthetics should not be considered general metabolic depressants without qualification.

Anesthesia, General↗
PubMed1984

Chromosome maps of man and mouse II.

Chromosome displays and listings are presented showing loci whose position is known in both man and mouse, in similar manner to our previous report (Dalton et al. 1981). There is now evidence for at least 27 conserved autosomal segments with two or more loci in the two species. The human and mouse chromosome maps show the location of homologous genes. The mouse map also shows the positions of translocations used in gene location and of some other genes used in linkage studies on them.

Animals↗
PubMed1984

Transverse topography of the photochemical reaction center polypeptides in the Rhodopseudomonas capsulata membrane.

The exposure of the three polypeptide subunits H, M, and L of the photochemical reaction center (RC) on both surfaces of the membrane of Rhodopseudomonas capsulata was studied by partial proteolysis with proteinase K and sodium dodecyl sulfate-polyacrylamide gel electrophoresis of of degradation products. The possible association of RC subunits with bacteriochlorophyll a and bacteriopheophytin was investigated by spectroscopical measurements. Chromatophores (inside-out oriented) and spheroplasts (right-side-out oriented), as well as purified, detergent-solubilized RCs and RCs reconstituted into phosphatidyl choline liposomes, were used. Subunit H of the RC was degraded to fragments with apparent MrS of 15,000 and 12,500, which were possibly derived from cleavage of a loop exposed on the cytoplasmic surface. Polypeptide M was digested at a comparable rate. The apparent Mr of M decreased by roughly 4,000 upon proteolytic cleavage. Subunit L was relatively insensitive to protease attack, except that a small peptide was clipped off. The primary donor P870 was also found to be only slightly affected proteinase K. All three RC subunits appear to be exposed on the chromatophore surface.

Antigen-Antibody Complex↗
PubMed1984

Clinical evaluation of a percutaneous pneumothorax catheter.

This study prospectively evaluates the efficacy and complications of the percutaneous pneumothorax catheter. Twenty-five patients were treated with the percutaneous catheter for 19 episodes of spontaneous or iatrogenic pneumothorax, six malignant effusions, and one infected bulla. The catheter was effective in all but a single episode of spontaneous or iatrogenic pneumothorax and was especially useful in three patients with tension pneumothorax. Further experience is required to define fully its feasibility in the treatment of malignant effusions or infected bullae. The ease of insertion, good response, and low incidence of complications suggest that this catheter may be a useful alternative to tube thoracostomy in selected situations.

Adult↗
PubMed1984

[How many biological indicators have to be tested to get reliable information on their resistance?].

Biological indicators are used in the efficacy test of microbicidal procedures. The indicators consist of an object carrying or holding micro-organisms which exhibit resistance to microbicidal agents. The biological indicators are exposed to the procedure to be tested and afterwards examined for viable germs. If test germs are still found to grow in the cultures, the microbicidal effect of the procedure is considered as insufficient. Biological indicators are suitable for such tests only if it is known how intensive the action of the microbicide has to be to destroy the test germs. The individuals of a germ population do not die at the same time under the action of a microbicide. This phenomenon can also be observed with germs simultaneously grown as a pure culture under identical conditions. Therefore, the biological indicators do not become sterile after one and the same period of action or dose of the microbicide but within a certain period or dose range. At the beginning of this transition range, sterile biological indicators will be found very rarely. With increasing period of action or dose, the frequency of indicators carrying viable germs decreases until, eventually, hardly any biological indicators with viable germs are detectable. When samples of identical biological indicators equal in size are exposed to one and the same period of action or dose of a microbicide, the number of indicators carrying viable germs will vary from one sample to another in the transition range. The number of biological indicators that has to be exposed to the resistance test per period of action and dose, respectively, in order to obtain reliable results, can be estimated only if the regularities are known by which the findings vary from one sample to another. Twelve different batches of biological indicators were employed to determine the variation of the resistance values obtained. Spores of Bacillus subtilis served as test germs. The batches differed in the number of spores per biological indicator. The microbicide used was saturated steam of 100 degrees C with a 9 min period of action. Forty-eight samples of five indicators each were taken per batch. The number of indicators carrying viable germs (n+) varied more or less and characteristic frequency distributions were observed (Table 2, columns 3 and 4). Afterwards, the mean relative frequency of indicators with viable germs was calculated for the different batches (Q; Table 2, column 2).(ABSTRACT TRUNCATED AT 400 WORDS)

Bacillus subtilis↗
PubMed1984

[Carotid aneurysm following apparent complications in catheterization of the internal jugular vein].

During resuscitation of a patient wih ruptured abdominal aortic aneurysm his right internal jugular vein was cannulated with a 12 gauge catheter-through-cannula assembly (Cavafix 458, Braun-Melsungen), using a high central approach, without any apparent complications. Five weeks later a dissecting aneurysm of the right common carotid artery developed. From clinical, angiographic and histological data it is very likely that the aneurysm resulted from the catheterization procedure.

Aortic Dissection↗
PubMed1984

Evaluation of hemoptysis in patients with a normal chest roentgenogram.

Hemoptysis is a common presenting complaint in patients with pulmonary disease. Although controversial, many clinicians suggest that all patients with hemoptysis should be evaluated with bronchoscopy to exclude the presence of a serious pathologic condition. We reviewed records for an 18-month period, during which 113 patients had hemoptysis as their principal complaint. In all, 26 of the 113 patients had normal chest roentgenograms. All underwent bronchoscopy as part of their evaluations. Results of bronchoscopy in these patients neither altered therapeutic decisions nor led to diagnoses of specific pathologic processes. We conclude that close observation of patients with normal chest roentgenograms who have hemoptysis may be an acceptable clinical approach.

Hemoptysis↗