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Biomedical subjects

J Pazdera

Publications and source records attributed to J Pazdera.

32 records · Page 2Linked to original sources
PubMed1982

The technique of ureterocystoneostomy in experimental renal orthotopic allotransplantation in the pig.

Experimental orthotopic renal allotransplantation performed in pigs allowed us to verify some techniques of ureterocystoneostomies from the urological point of view. For the reconstruction of the urinary pathways we employed anastomosis in 7 cases, and implantation of the ureter into the bladder in 3 cases, using the antireflux technique in 9 animals and the now common ureteral implantation with the terminal segment of the ureter placed in tunnel serosa in 24 animals. The results of our modification confirm the importance of peritoneal plasty of the terminal ureteral segment for the prevention of urinary fistulas. This technique seems to be applicable to reconstruction of urinary pathways in humans.

Animals↗
PubMed1981

The presence of blood group and lymphocyte antigens on porcine granulocytes.

Suspensions of highly viable (less than 95%) granulocytes minimally contaminated by other cell types were isolated from the peripheral blood of pigs by a single centrifugation with low molecular weight dextran and after preferential lysis of erythrocytes by hypotonic shock. A complement-dependent cytotoxic test showed the presence of antigens of the SLA major histocompatibility complex, the SLB leucocyte system and the A and E blood group systems on the granulocytes. Some SLA typing reagents against class I (SD) antigens did not react with granulocytes, however, or yielded dubious reactions. The findings showed that the reactivity of SLA sera resembles the reactivity fo human HLA sera. The results also show that compatibility in the SLA, SLB, A and E systems will have to be taken into account when preparing alloimmune sera for the determination of granulocyte-specific antigens of pigs.

Animals↗
PubMed1981

The effect of haemagglutinating factor from boar seminal vesicle fluid on leucocytes.

Complete agglutination of porcine, bovine, ovine, and rabbit leucocytes and of porcine and bovine thrombocytes was observed after their exposure to a 1% solution of the haemagglutinating protein isolated from boar seminal fluid. Bull seminal vesicle fluid had the same agglutinating effect on leucocytes, but did not agglutinate thrombocytes. Ram seminal plasma and other fluids from the reproductive tract of boar and bull did not agglutinate either leucocytes or thrombocytes. A viability test showed that the agglutinin of boar seminal vesicle fluid, bull seminal vesicle fluid, and boar prostate fluid were all lethal for leucocytes.

Agglutination↗
PubMed1981

Immunogenetic studies in semi-inbred pigs.

Seventy-six siblings from 11 semi-inbred families of Landrace pigs (inbreeding coefficient 0.70-0.82) were used for transplantation of skin and renal allografts. The animals were selected with regard to the major histocompatibility complex SLA, alloantigenic systems SLB, SLC and blood groups. Using the test of the difference in mean level between two groups of observations, the influence of compatibility in the E blood group system on the survival of transplants was proved. The observed difference suggests that the E blood group complex or a linked locus behaves as a minor histocompatibility system. The induction of tolerance in piglets treated and non-treated with allogeneic sera from long-term liver recipients was also investigated. Large doses of sera did not exert any effect on skin graft survival.

Alleles↗
PubMed1979

The blood group factor Kf and allele Kae in the pig.

By means of alloimmune reagents used in the indirect Coombs test and the dextran test a new factor Kf in the K bloodgroup system of pigs was found, controlled by alleles Kacf, Kacef and Kbf. A new allele Kae was also detected. The K system with 6 alleles, 11 phenotypes and 21 combinations of genotypes remains (from the genetic point of view) an open system.

Alleles↗
PubMed1978

SLB, a new alloantigenic system of the pig.

An alloantigen segregating in the double back-cross families, distinct from serologically determinalble transplantation antigens controlled by the major histocompatibility complex (SLA), was detected in pigs by the complement-dependent lymphocytotoxic technique. The system was designated SLB and its product (antigen SLB-A1) was demonstrated in all lymphocytes from the lymph nodes, thymus and peripheral blood, but not in the erythrocytes. Back-cross matings supplied evidence of the dominant heredity of SLB-A1. Detection of further SLB antigens was unsuccessful, so that only two alleles (SLB-A1 and SLB-A-) could be determined in families. Double back-cross families revealed a close linkage between the SLB locus and the L blood group locus. Maximun lod score values were calculated in recombination fraction theta=theta.10.

Animals↗
PubMed1977

Serologically defined specificities of the pig main histocompatibility complex (SL-A).

Six antigens detectable by the complement-dependent lymphocytotoxic technique were determined in pigs by six groups of alloimmune sera. It was confirmed that these specificities are controlled by the main histocompatibility region (SL-A). Serological and genetic studies showed that the given specificities (provisionally designated L1 to L6) form at least 6 haplotypes. In addition, family studies confirmed the linkaged between SL-A region and C blood group locus. Maximum lod score values are in recombination fraction omicron = 0.2.

Animals↗