Sensitization to penicillin was determined in vitro in twenty subjects, 30 days after cutaneous side-effects due to ampicillin. The study inclued the detection of anti-penicillin IgE, IgM, a lymphoblastic transformation test and a leucocyte migration test. No difference could be demonstrated between the sensitization 30 days after an ampicillin reaction and the sensitization 30 days after a penicillin reaction.

Adult↗

PubMed1976

Value of lymphoblast transformation test in cow's milk protein intestinal intolerance.

Lymphoblast transformation tests were carried out in the presence of alpha-lactalbumin and beta-lactoglobulin. In patients with cow's milk protein intestinal intolerance in seventeen of forty-five (37.8%) the lymphoblast transformation tests were positive. Sensitization in the first month of life seemed to favour lymphoblast transformation. In control children in only four of forty-three (9.5%) the lymphoblast transformation tests were positive, the difference from intolerant patients being significant 0.01 less than P less than 0.001. Lymphoblast transformation tests were negative in the seven children with active coeliac disease. Although a negative test does not exclude cow's milk protein intolerance, lymphoblast transformation tests can be considered a useful aid in diagnosis because of its specificity.

Allergens↗

PubMed1976

Lymphocyte transformation, leucocyte migration, specific IgE, IgG and IgM before, during, and after penicillin treatment without adverse reaction. A follow-up study.

This in vitro follow-up study of the penicillin-induced immune response by a well-tolerated regimen was conducted with an association of four tests: lymphocyte transformation test; leucocyte migration test; passive anaphylaxis on monkey lung tissue; and passive hemagglutination. It showed (1) that previously sensitized subjects (including IgE) tolerated the treatment by pencillin. (2) That the treatment induced either a transient positive conversion of previously negative tests or a negative conversion of previously positive tests. These in vitro findings are in accordance with previously established concepts based upon cutaneous tests and must be taken into consideration when interpretating in vitro diagnosis of penicillin allergy.

Adolescent↗

PubMed1976

The penicillin antigens: presentation in the leucocyte migration test (a study with penicillin and penicilloylated proteins).

The response of the leucocyte migration test (LMT) to benzyl penicillin (BP) was compared with the response of LMT to benzyl penicilloylated human gamma globulins (BPO HGG) and to benzyl penicilloylated human serum albumin (BPO HSA). Patients with and without hypersensitivity to penicillins were investigated. No statistical differences were found between the response to BP and to BPO HGG or BPO HSA; this finding supports the fact that the BPO groups generated by BP are mainly implicated in the triggering of the LMT when BP is used as antigen. A strongly penicilloylated HSA appeared to be the most effective mode of the antigen presentation for the detection of BPO-specific cellular immunity in patients with and without clinical symptoms of hypersensitivity to the penicillins.

Adult↗

PubMed1976