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J Pasternak

Publications and source records attributed to J Pasternak.

At least 55 records · Page 3Linked to original sources
PubMed1975

Properties and synthesis of ribosomal RNA in the free-living nematode Panagrellus silusiae.

The normal S values of ribosomes and ribosomal subunits extracted from the free-living nematode. Panagrellus silusiae are similar to those of other eukaryotic organisms. Upon heating (70 degrees C, 15 min) a 5.5-S RNA is released from the larger ribosomal RNA (27 S; 1.40-10(6) daltons). Unlike other protostomes, however, the 27-S rRNA does not dissociate in an orderly manner to yield 18-S components. The electrophoretic mobility of the small ribosomal RNA species (18 S; 0.72-10(6) daltons) is unaltered by heat treatment. The base composition of both Panagrellus rRNA species has a 51% G+C content. Labelling worms with either [Me-3H] methionine or [14C] uridine at various stages of postembryonic development and subsequent analysis of the extracted RNA revealed eight radioactive fractions, two of which were the mature ribosomal RNAs. From both the time sequence of appearance of the eight RNA species and the relative degree of methylation of the various RNA fractions two maturation pathways for ribosomal RNA processing were deduced. A 37-S (3.6-10(6) daltons) RNA is the precursor to both 27-S and 18-S RNA. During processing about two-fifths of the original transcriptional unit is lost. In this organism the "transcribed spacer" regions may be methylated although alternate interpretations have not been precluded.

Cell Fractionation↗
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Emergent infections.

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