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Biomedical subjects

J Otto

Publications and source records attributed to J Otto.

At least 73 records · Page 4Linked to original sources
PubMed1989

Methemalbumin in acute pancreatitis: an evaluation of its prognostic value and comparison with multiple prognostic parameters.

Methemalbumin (MHA) and Ranson's prognostic parameters were measured in 90 patients with acute pancreatitis. Thirteen died (14.4%). The mortality rate was 36% in MHA-positive versus 6.2% in MHA-negative patients. Severe renal (56% vs 18.5%) and pulmonary (44% vs 10.2%) complications occurred more frequently in the MHA-positive as compared with the MHA-negative group. Hemorrhagic pancreatitis was found in all MHA-positive patients who were subject to surgery or postmortem examination. By way of comparison, the presence of more than four Ranson signs was associated with about the same risk of mortality and the development of complications as positive MHA findings. There was a good correlation between single and multiple prognostic factors for slight but not for moderate-severe or severe cases.

Acute Disease↗
PubMed1988

Detection of pancreatic steatorrhea by oral pancreatic function tests.

Pancreolauryl and NBT-PABA tests were performed in urine of 54 patients with exocrine pancreatic insufficiency and, additionally, in serum of 29 of these patients. All patients underwent a secretin-pancreozymin test and a 72-hr fecal fat analysis. Pancreatic steatorrhea occurred (with only three exceptions) when the pancreolauryl test revealed a T/C ratio [recovery of the fluorescein of the test (T) and the control (C) day] of less than 10, or when serum fluorescein concentrations were below 0.5 microgram/ml. The NBT-PABA test also showed a negative correlation between urinary PABA excretion or serum PABA concentration and fecal fat excretion, but there was no diagnostically useful cutoff limit indicating decompensation of exocrine pancreatic insufficiency. These findings indicate that the pancreolauryl test may facilitate clinical evaluation of patients with chronic pancreatitis by simultaneously assessing exocrine pancreatic insufficiency as a cause and predicting pancreatic steatorrhea as a sequel of maldigestion. In clinical practice, the pancreolauryl test can be used as a parameter for deciding whether to initiate pancreatic enzyme substitution if direct pancreatic function tests and fecal fat analysis are not available.

4-Aminobenzoic Acid↗
PubMed1988

When should treatment of acute experimental pancreatitis be started? The early phase of bile-induced acute pancreatitis.

Sodium taurocholate pancreatitis in the rat is a frequently used experimental model for evaluating therapeutical regimes in this disease. It is, however, uncertain when treatment should be started, as the early phase of this experimental model and thus the time when the pancreatitis really develops is unknown. Serum and pancreatic enzymes, as well as pancreatic morphology, were therefore studied 5, 30, and 60 min after induction of sodium taurocholate pancreatitis. It was found that increase in serum enzymes and decrease in pancreatic enzymes and morphological changes characteristic for acute pancreatitis develop as early as 5 and 30 min after induction of pancreatitis. Thus, therapy in this model may be started shortly after induction of acute pancreatitis.

Acute Disease↗
PubMed1988

Identification and subcellular location of talin in various cell types and tissues by means of [125I]vinculin overlay, immunoblotting and immunocytochemistry.

In the present study, we have examined the cellular and subcellular distribution of talin in several tissues of the chicken. By immunocytochemistry, Western Blot analysis and [125I]vinculin overlay, talin was demonstrated in most of the main tissues and cell types of the body. Corresponding to the property of talin to bind to the fibronectin receptor, talin was found to be confined to the site of the plasma membrane that abuts the extracellular matrix in various types of mesenchymal and epithelial cells. In the central nervous system talin was almost exclusively confined to cells of the connective tissue, i.e., blood vessels and the connective tissue sheaths. No evidence was obtained for the association of talin with any type of intercellular junction. In nonadhering cells such as circulating platelets and leukocytes, talin displayed a diffuse distribution throughout the cytoplasm. These findings suggest a general role for talin in certain aspects of cellular adhesion to the extracellular matrix.

Animals↗
PubMed1987

Influence of buprenorphine on acute experimental pancreatitis.

Buprenorphine (15 micrograms/kg b.wt. per hour) distinctly reduced pain sensitivity in acute 3% sodium-taurocholate pancreatitis in male Wistar rats without interfering with the course of the disease. This was seen by assessment of enzyme elevation in serum and ascites and by histological evaluation of the pancreas. Buprenorphine is therefore recommended for animal experiments to study the effect of therapeutic principles in acute pancreatitis.

Acute Disease↗
PubMed1987

Subcutaneous administration of the synthetic trypsin inhibitor Foy-305 induces hypertrophy of the exocrine pancreas.

The serine protease inhibitor camostate (Foy-305; 200 mg/kg body weight) had been administered twice daily either subcutaneously or orally to mice for 5, 10 and 15 days. Within 5 days, pancreatic weight, concentration of trypsin, amylase and protein were significantly increased and even more increased after 10 and 15 days. This effect is less pronounced after subcutaneous administration in comparison to oral treatment.

Administration, Oral↗
PubMed1987

Serological screening for allergy to inhalants by multi-allergen-radioimmunoassay.

201 children with inhalant allergy were examined with a new serological screening test ("Multi-Allergen-Test"). The sensitivity (95.1%) and specificity (91.9%) of the test were determined by comparing it to a "clinical classification", including results of history, physical examination, skin test and investigation for specific IgE antibodies. This yielded a diagnostic efficiency of 93.5%. A subsequent comparison of these results with the classification of patients by age-related total IgE levels showed the superiority of the "Multi-Allergen-Test" as far as screening for inhalant allergies is concerned. Additionally, the results for the specificity of the test were reproduced by examining another 18 children with food allergy, who had elevated total IgE levels but no inhalant allergic symptoms. The "Multi-Allergen-Test" proved to be a suitable screening procedure for inhalant allergy.

Allergens↗
PubMed1986

Salivary isoamylase in duodenal aspirates.

Salivary isoamylase was measured in duodenal aspirates of 124 consecutive patients during a secretin-pancreozymin test, using an inhibitor method. The isoenzyme was found in 22 patients (17.7%), more often in the patients with abnormal pancreatic function (40.5%) than in those with normal function (8.0%). Before the subtraction of the salivary isoamylase component, total amylase output was falsely normal in three of 37 patients with exocrine pancreatic insufficiency. It is concluded that measurement of amylase isoenzymes rather than of total amylase during the secretin-pancreozymin test may be recommended for scientific purposes. It may also improve discrimination between normal and abnormal exocrine pancreatic function if amylase is chosen as the only parameter for evaluation of pancreatic enzyme secretion.

Adolescent↗
PubMed1986

Pancreatic calcifications: no indicator of severe exocrine pancreatic insufficiency.

Pancreatic calcifications are believed to occur only in advanced stages of exocrine pancreatic insufficiency. This belief has been reevaluated by correlating the results of the secretin-pancreozymin test, fecal fat analysis, and the presence of pancreatic calcifications on plain abdominal x-rays in 79 patients with chronic pancreatitis. Exocrine pancreatic insufficiency was classified as slight, moderate, or severe according to the results of the function tests, and pancreatic calcifications were assessed semi-quantitatively (grades 1-3) by an independent examiner. The results showed that severe exocrine pancreatic insufficiency did occur even in the absence of calcifications. Calcifications were more frequently detected with increasing severity of exocrine pancreatic insufficiency. The qualitative demonstration of pancreatic calcification was, however, not an indicator of severe, decompensated exocrine pancreatic insufficiency (50% false-positive results). It is concluded that pancreatic calcification is not necessarily an indicator of severe exocrine pancreatic insufficiency, and vice versa. Comparison between the results of tests for endocrine pancreatic function and plain abdominal x-ray showed such similarity that it can also be concluded that pancreatic calcifications are no indication of abnormal endocrine function, and vice versa.

Calcinosis↗
PubMed1986

Protein binding of nifedipine.

The protein binding of nifedipine in concentrations up to 1200 ng ml-1 has been measured in serum, pure human albumin solution and pure human alpha 1-acid glycoprotein (AAG) solutions by ultrafiltration. The drug was extensively bound in serum from four healthy volunteers with a mean (+/- s.d.) fraction bound of 0.992 +/- 0.008. In albumin solution (40 g litre-1) the mean (+/- s.d.) fraction bound 0.970 +/- 0.012, was not significantly different (P greater than 0.05) from that in serum, suggesting that albumin is the major, but not necessarily the only, binding protein for nifedipine in serum. The binding of nifedipine in solutions of AAG was proportional to the AAG concentration and ranged from 0.514 +/- 0.059 to 0.755 +/- 0.035 in solutions containing 50 and 150 mg % AAG, respectively. Binding of nifedipine in all protein solutions was linear.

Adult↗
PubMed1986

Pancreolauryl and NBT-PABA tests. Are serum tests more practicable alternatives to urine tests in the diagnosis of exocrine pancreatic insufficiency?

Serum fluorescein and p-aminobenzoic acid were measured during a urine pancreolauryl and an N-benzoyl-l-tyrosyl-p-aminobenzoic acid (NBT-PABA) test in 22 healthy controls, 17 patients with gastrointestinal nonpancreatic diseases (normal secretin-pancreozymin test), and 31 patients with abnormal exocrine pancreatic function due to chronic pancreatitis. The optimal cutoff point for separating normal from abnormal pancreatic function was after 210 min in the pancreolauryl test and after 150 min in the NBT-PABA test. The latter test was slightly less sensitive and specific than the pancreolauryl test. Serum tests seem to offer a practicable alternative to the established indirect pancreatic function tests in urine and may be used in the elderly and severely ill, as well as in outpatients in whom correct collection of the urine may be difficult.

4-Aminobenzoic Acid↗
PubMed1986

Estimation of serum pancreatic isoamylase: its role in the diagnosis of exocrine pancreatic insufficiency.

Using an inhibitor method, fasting levels of pancreatic isoamylase were measured in 46 healthy controls and in 218 patients undergoing a secretin-pancreozymin test for diagnostic purposes, and compared with immunoreactive trypsin (IRT). Exocrine pancreatic insufficiency was found in 82 of the patients. The specificity of both enzymes was high in patients with nonpancreatic diseases (pancreatic isoamylase: 98.5%, IRT: 96.3%). No patient with nonpancreatogenic steatorrhea had a low serum enzyme value. Sensitivity was, unfortunately, low in patients with exocrine pancreatic insufficiency (pancreatic isoamylase: 45.1%, IRT: 41.5%) and increased only slightly when patients after an acute attack of the disease, or patients with pseudocysts or older than 60 were excluded (pancreatic isoamylase: 67.9%, IRT: 58.5%). Although highly specific, pancreatic isoamylase measurement is not sensitive enough to be used as a screening test for exocrine pancreatic insufficiency but may be used to determine the etiology of steatorrhea.

Cholecystokinin↗
PubMed1985

[Development of a questionnaire for problem solving].

Presented is the development of a measuring instrument in order to assess the problem solving ability (PLF). The PLF consists of 5 scales: a) experiencing problems, b) problem denial, c) tendency to solve problems in an unconventional way, d) dealing with the problem and e) tendency to conservative problem solving. Test-theoretical analyses are reported as well as, in greater detail, studies for test validation on various populations. High covariations can be shown especially for personality factors.

Adaptation, Psychological↗