Search PubMed⌕ Search

Biomedical subjects

J Okada

Publications and source records attributed to J Okada.

At least 199 records · Page 11Linked to original sources
PubMed1983

Immunological study of IgA deficiency during anticonvulsant therapy in epileptic patients.

Of 10 epileptic children with IgA deficiency, one showed normal IgA synthesis and secretion on in vitro pokeweed mitogen (PWM) stimulated lymphocyte culture in contrast to IgA deficiency in vivo, two showed IgA synthesis in cytoplasm without any release of IgA into the supernatant and seven failed to synthesize IgA. Co-cultures with allogeneic T or B cells in various combinations with PWM showed intrinsic IgA-B cell defect without T cell defect in two of the second group affected at IgA secretion and in five of the third group, and intrinsic IgA-B defect with dysfunction of T cells in two of the third group. Thus, the IgA deficiency in these epileptic patients was demonstrated to be heterogenous.

Adolescent↗
PubMed1983

The effect of bifonazole on the structure of Trichophyton.

The effect of a new azole antimycotic, 1-[(4-biphenylyl)-phenylmethyl]-1H-imidazole (bifonazole, Bay h 4502, Mycospor) on the morphology of hyphae of Trichophyton mentagrophytes grown in a liquid medium was studied by high-resolution scanning electron microscopy. With bifonazole at concentrations ranging from 1 to 10 ng/ml that were much lower than the MIC value of the drug for this test strain (630 ng/ml), the hyphal growth was significantly inhibited and several morphological changes of the hyphae were produced. They were characterized by: (1) development of wavy or curled hyphae, (2) occasional formation of swollen cells often arranging in chain; (3) partial exfoliation of hyphal walls; and (4) excretion of fibrillar materials. These morphological changes of the hyphae became more prominent with increasing drug concentrations up to 200 ng/ml, and many hyphae were collapsed and distorted. These results strongly suggest that subinhibitory concentrations of bifonazole profoundly affected the normal growth and induced degenerative changes of the hyphae of T. mentagrophytes probably by damaging the cell membrane.

Antifungal Agents↗
PubMed1982

Alloantigens expressed on a subset of unstimulated human T lymphocytes that generate suppressor function.

Human alloantisera were tested for antibodies reacting with T-cell subpopulations. T-cell subsets were separated using the monoclonal antibodies OKT4 and OKT8. Five sera reacting with the T4-T8+ subset and two sera reacting with T4+T8- lymphocytes were identified. Serum Z. G. reacted with T4-T8+ cells from 8 of a panel of 19 donors. T cells treated with Z. G. serum and rabbit complement lost the capacity to generate suppressor cells but showed no decrease in the development of cytotoxic effector cells. ZG antigens were demonstrated by absorption also on monocytes but not on B cells. Their reactions on T cells were blocked by chicken anti-human Ia serum, but not by turkey anti-beta 2-microglobulin or by a monoclonal anti-human DR (L227). Studies in four informative families suggested that the ZG determinants are inherited in linkage with HLA. Although the similarities between ZG antigens and mouse I-J products are striking, structural studies are needed to establish their homology.

Antibodies, Monoclonal↗
PubMed1982

A simple bile-disk method for the identification of Bacteroides fragilis.

A simple method for the separation and identification of Bacteroides fragilis from other Bacteroides species through the use of oxgall-impregnated filter paper disks is described. As in the antibiotic sensitivity test, filter paper disks containing defined amounts of bile salt were placed on a lawn of bacterial cells on Gifu anaerobic medium agar plates. Bile-resistant bacteria were identified as strains of Bacteroides fragilis after incubation in anaerobic jars containing hydrogen and carbon dioxide generators at 35 C for 24 hr. The optimum concentration of bile salt, yielding results very similar to those of the conventional tube method, was determined to be 25 mg per disk, empirically. Since this method is easy to perform, time saving, economical and gives clear results, it may be readily used as a daily routine test in the clinical microbiology laboratory.

Bacteriological Techniques↗
PubMed1982

Therapeutic effects of tiopronin on chronic hepatitis: a double-blind clinical study.

The efficacy of tiopronin (2-mercaptopropionyl glycine) 600 mg daily in the treatment of chronic active or chronic persistent hepatitis has been assessed in a double-blind controlled clinical trial of 12 weeks involving 165 Japanese patients with histologically proven disease. Treatment with the drug was associated with a significant improvement in abnormalities of serum transaminase and gamma glutamyl transpeptidase, with reversion towards baseline values on stopping the drug. Improvement was independent of the histological classification of the disease, or HBsAg status. The drug was well tolerated with few side-effects. The results of this short-term study indicate that tiopronin may be of value in the treatment of chronic hepatitis.

Acyltransferases↗
PubMed1982

Search for the central origin of efferent fibers in the carotid sinus nerve of the cat.

Using the horseradish peroxidase (HRP) technique, the central origin of efferent fibers in the carotid sinus nerve (CSN) was examined in the cat. To avoid false labeling due to uptake from the nerve endings in the adjacent tissues of the CSN, the critical amount of HRP was sought, which was estimated at 0.5 mg. Applying 0.5 mg of HRP to the central cut end of the CSN, many labeled cells were found in the petrosal ganglion, but no labeled cells were found in the central structure. The findings of this study indicate that the origin of the most efferent fibers in the CSN is not in the central structure, but that it may be in the extracranial part of the glossopharnygeal nerve.

Animals↗
PubMed1982

[Change of pattern of gastric emptying after selective proximal vagotomy].

The patterns of gastric emptying after selective proximal vagotomy (SPV) and SPV with pyloroplasty (SPV + D) were studed using a liquid labelled with 99mTc-DTPA. By means of on-line computer facility, it was possible to analyse the relative role of the Whole stomach and the antrum within the overall frame work of gastric emptying. In the time/activity curve of the whole stomach, all of 3 groups emptied in exponential curve. Comparing with normal subjects, SPV subjects were tending to empty slowly, while SPV + D subjects were tending to empty rapidly. In the time/activity curve of the antrum, normal and SPV + D subjects had two types of emptying--one was represented in the decreasing curve and another was represented in the increasing-decreasing curve. While SPV subjects had only one type of emptying which was represented in the increasing-decreasing curve. From a consideration of the pattern of gastric emptying curve, SPV subjects had two different emptying states compared with normal subjects--one was the delay of entering the antrum from the fundic side of stomach, and the other was that effective antral emptying did not readily start. While SPV + D kept more neally normal gastric emptying that it of SPV.

Adult↗
PubMed1981

Factors influencing the development of Moyamoya phenomenon.

Both the Moyamoya Phenomenon and occlusion of the internal carotid fork are essential radiological findings in true Moyamoya Disease of unknown aetiology. However, the Moyamoya Phenomenon is often observed in occlusive diseases of the internal carotid bifurcation of known aetiology. The authors recently observed acute development of the unilateral Moyamoya Phenomenon following severe vasospasm of the anterior and middle cerebral arteries due to rupture of an anterior communicating aneurysm. The following four factors have been suspected of contributing to development of the Moyamoya Phenomenon: 1. The chronology of arterial occlusion. 2. Extent and location of occlusion. 3. The cause of occlusion. 4. Anatomical and functional disposition of the basal circulation. As regards the chronology, chronic or slowly progressive arterial stenosis has been thought to be a mandatory factor in development of a Moyamoya network, which plays an important role in the form of collateral channels. However, based on the findings outlined in this paper, the congenital factor may be the most important of the four factors.

Arterial Occlusive Diseases↗
PubMed1981

Familial basal ganglia calcifications visualized by computerized tomography.

Intracranial calcification can now be detected easily and precisely with the advent of computerized tomography. A familial case of striopallidal calcification with a rare hereditary pattern of autosomal dominancy is presented. None of the family members, aged from 8 to 62, displayed any neurological abnormality. All female family members had, bilaterally, short fourth metatarsals. Serum calcium and phosphorus values were not abnormal, although such physical findings are compatible with pseudohypoparathyroidism. The family tree suggested autosomal dominant heredity with a penetrance rate of 100%. Our survey revealed that no more than 10 cases of familial striopallidal calcification excluding ours have been reported to date. Only by utilizing CT was the hereditary pattern of our case determined accurately.

Adolescent↗