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Biomedical subjects

J Morenz

Publications and source records attributed to J Morenz.

At least 19 recordsLinked to original sources
PubMed1991

[Determination of thyroglobulin autoantibodies using enzyme immunoassay, radioimmunoassay and passive hemagglutination].

A solid-phase enzyme immunoassay, a radioimmunoassay and the passive hemagglutination were tested for the determination of antithyroglobulin autoantibodies. The antibody-titres detected with these methods showed a good correlation. The sensitivities of the solid-phase immunoassays are four times higher than the sensitivity of the passive hemagglutination. For the diagnosis of thyroid autoimmune diseases the enzyme immunoassay.

Autoantibodies↗
PubMed1989

[Axial filament antigen enzyme immunoassay (AF-ELISA) in syphilis serology].

179 sera from patients with various stages of syphilis were investigated in the axial filament enzyme linked immunosorbent assay (AF-ELISA), the Treponema pallidum haemagglutination assay (TPHA), the fluorescence treponemal antibody absorption (FTA-ABS) test and the cardiolipin micro precipitation test (CMT). For the control of specificity 30 problem sera from non Treponema pallidum infected patients with a high quantity of Treponema genus specific antibodies and 40 sera from people without syphilis were also investigated. The sensitivity of the AF-ELISA (98.3%) was comparable with the TPHA (99.4%) and the FTA-ABS-test (98.9%). It was a high unspecificity in the group of problem sera.

Antigens, Bacterial↗
PubMed1989

[Characterization of treponemal axial filament antigens using the Western blot].

Analysis by SDS-PAGE of axial filaments of cultivable treponemes showed 3 major bands: a 33/34 kD doublet and a 37 kD polypeptide. In sera from patients with various stages of syphilis, from biological false positive reactors, and from negative controls the most consistent reaction was detected against the doublet, but only sera from patients with primary or secondary syphilis reacted with the 37 kD axial filament polypeptides. About 6 weeks after antibiotic treatment sera had lost their reactivity with 37 kD antigens in agreement with decreasing antibodies in VDRL-test. Because of their recognition early in infection 37 kD-proteins are postulated to use in serodiagnostic assays, but our data emphasize also the need for use further antigens in tests for diagnosis in later stages of syphilis.

Antigens, Bacterial↗
PubMed1989

[Incidence and significance of humoral antibodies in females with recurrent spontaneous abortions].

Serum samples from 70 patients with two or more early spontaneous abortions and, for comparison, sera from 20 healthy and fertile women were assayed by indirect immunofluorescence for the presence of antibodies to sperms and genital organ antigens and for non-organ specific and organ specific antibodies. All sera were tested for thyreoglobulin antibodies by hemagglutination. The frequency of sperm antibodies and autoantibodies was significantly higher in patients with recurrent abortions than in the control group. There was no relation between the type or titer of antibodies and the clinical data of the patients.

Abortion, Habitual↗
PubMed1988

[Clinical, immunologic and therapeutic aspects of endangiitis obliterans].

In the period of 1975-1983 twenty-three patients with thromboangiitis obliterans were examined at the Surgical Clinic of the Medical Academy of Magdeburg. The diagnosis was established clinically, angiographically histologically and immunologically. In 7 out of 12 patients histology revealed inflammatory vascular alterations including lymphocyte infiltrates throughout the entire vessel wall and also in the perivascular area. Immunohistology showed in 10 out of 15 patients segmental granular fluorescence identifiable as deposits of IgM and IgG. In 6 cases a complement formation was found. The endangiitis group revealed more frequent and increased immune complexes concentrations. Circulating immune complexes were established by phase-locked radio-immunoassay. The therapy is depended on the localization of the obliteration and can be a lumbar sympathectomy or a vascular reconstruction.

Adult↗
PubMed1988

[Determination of immune complexes in the serum of pregnant patients and puerperal females with EPH gestosis].

We analyzed the blood sera from 100 patients with EPH gestosis and from 50 women with normal pregnancy to explore the immunological reactions during gestosis. The sera of cord blood were included in the investigations. We determined the circulating immune complexes by conglutinin and C1q solid phase radioimmunoassay and by polyethyleneglycol precipitation. It has been established by conglutinin RIA that during pregnancy the women with gestosis have significant higher concentrations of immune complexes than the control group. During puerperium and in the cord blood the values are significantly lower than during pregnancy in both groups. The estimations of immune complexes by C1q RIA have shown that the differences between the two groups are insignificant and the lowest values are in the cord blood. The concentrations of immune complexes estimated by PEG precipitation were not significantly different between the two groups. It is concluded that immune complexes are in connection with the pathogenesis of preeclampsia.

Adult↗
PubMed1987

[Detection and differentiation of autoantibodies to extractable nuclear antigens with immunoenzyme tests].

Nuclear antigens were extracted from calf thymus with phosphate-buffered saline The 60-80% ammonium sulfate fraction (ASF) of thymus extract contained SS-B-, the 30-60% ASF Sm- and U1-RNP-, and the chromatographically purified 30-60% ASF only Sm antigen. With these fractions enzyme immunoassays were developed for quantitative determination of Sm-, U1-RNP-, and SS-B autoantibodies. Out of 144 sera with antinuclear antibodies 85% were positive in the enzyme immunoassays, 41% in immunofluorescence tests on liver sections (12% speckled, 29% homogeneous immunofluorescence pattern), and 10% in Ouchterlony tests. 26% of the sera were positive in enzyme immunoassays and immunofluorescence tests. All antibody specificities detected by immunodiffusion could be confirmed by enzyme immunoassay. The enzyme immunoassay is far more sensitive then immunodiffusion and in contrast to immunofluorescence allows antibody specificities to be determined. Enzyme immunoassays are recommended for the diagnosis of rheumatic diseases.

Antibodies, Antinuclear↗
PubMed1987

[Effect of drugs on superoxide formation by neutrophilic granulocytes].

The in-vitro influence of drugs on the superoxide production of human neutrophils was investigated. The superoxide production is inhibited by colchicine, g-strophanthin, propranolol, fluphenazine and stimulated by levamisole and lithium. The superoxide production is inhibited or stimulated by chloramphenicol, hydrocortisone and amphotericin B in concentration dependent manner.

Amphotericin B↗
PubMed1987

[Detection of circulating immune complexes in patients with EPH gestosis or intrauterine fetal growth retardation].

Sera from 19 patients with EPH gestosis, 22 patients with hypotrophic newborns, and a control group of ten women with normal pregnancy and fetuses were studied for the presence of circulating immune complexes. Both the C1q solid-phase radioimmunoassay and the polyethylene glycol precipitation test revealed significantly higher levels of circulating immune complexes in both groups of patients.

Antigen-Antibody Complex↗
PubMed1986

[Pathogenetic mechanisms in autoimmune diseases].

In addition to an earlier review [Z. ges. inn. Med. 35 (1980) 58] newer concepts in the pathogenesis of autoimmune diseases are presented. Acantholysis in pemphigus is caused by proteases released from cells of stratified squamous epithelium stimulated by antibodies to cell membrane antigens. The dermoepidermal cleavage in pemphigoid is presumably due to proteases from granulocytes accumulating at the dermoepidermal junction by antibody-mediated complement activation. Receptor autoantibodies can directly stimulate or block receptor function, increase cell membrane receptor degradation, or activate complement with lysis of receptor-carrying membrane structures. Tissue injury in immune complex diseases is caused by reactive oxygen metabolites and proteases released from stimulated phagocytes.

Autoantibodies↗
PubMed1986

[Animal experiment studies of the modification of pregnancy by heterologous antibodies to placental antigens].

Pregnancy impairment in mice with abortion and fetal resorption was caused by rabbit antibodies to total mitochondrial, mitochondrial membrane and microsomal fractions of mouse placentas injected intraperitoneally at 12-14 days of gestation. Antibodies to cytosol and soluble mitochondrial constituents were ineffective. According to the marker distribution in the biologically active and inactive fractions membrane-bound antigens are thought to be responsible for the abortigenic effect.

Abortion, Spontaneous↗
PubMed1985

[Effect of drugs on granulocyte motility].

The in-vitro influence of drugs on the chemokinesis and chemotaxis of neutrophils was investigated in order to prevent additional drug-induced motility impairment of cells in cases of already existing host defense disorders and for an eventual specific treatment of motility defects. Granulocyte motility is unimpaired by penicillin, ampicillin, carbenicillin, streptomycin, nystatin, and cyclophosphamide. The chemokinesis and chemotaxis of neutrophils are inhibited by erythromycin, oxytetracycline, doxycycline, chloramphenicol, hydrocortisone, g-strophanthin, digoxin, and digitoxin and in higher concentrations also by sulfonamides, gentamycin, prednisolone, methylprednisolone, dexamethasone, and phenylbutazone. Chemotaxis is selectively or rather more inhibited than chemokinesis by amphotericin B, griseofulvin, vinblastine++, trifluoperazine, and promethazine. Granulocyte motility is, however, stimulated by ascorbic acid, potassium thiocyanate, levamisole, lithium, and metofenazate.

Anthelmintics↗
PubMed1984

Pregnancy impairment in mice by antibodies to subcellular placenta fractions.

The administration of rabbit antibodies to whole mitochondria, mitochondrial membranes, or microsomes of mice placentas at 12-14 days of gestation resulted in abortion or fetal resorption, whereas antibodies to administered cytosol, mitochondrial matrix, or the mitochondrial intermembrane fraction were ineffective. From the distribution of marker enzymes in the biologically active and inactive fractions the abortigenic antigens are thought to be membrane-associated. Because of insurmountable cross-reactivities of the heterologous antisera, the immunopathogenesis of abortion could not be adequately clarified. However, preliminary experiments with antibodies (immune ascites) from mice immunized with the mitochondrial-microsomal fraction proved successful. A homologous animal model might be more helpful in elucidating the role of antibody-mediated immune mechanisms in human pregnancy impairment.

Abortion, Spontaneous↗
PubMed1981

Immunological factors in human male infertility: immunopathological studies of testes.

Biopsy specimens of human testes from 190 men of infertile couples were treated with fluorescein-labelled antibodies to demonstrate tissue-bound IgG, IgA, IgM, IgD, C3, and C4. In 24 cases (12.6%) autoantibodies and/or complement could be detected. Most often IgG bound to walls of seminiferous tubules was found. Three men had antibodies or complement in germinal cells. In one case each IgA immunofluorescence was found in spermatids or Leydig cells. The results suggest that autoantibodies to testicular antigens may be implicated in the pathogenesis of some cases of infertility in man.

Adolescent↗