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Biomedical subjects

J Monahan

Publications and source records attributed to J Monahan.

78 records · Page 5Linked to original sources
PubMedDate not supplied

Gene transfer into rat renal cells using adeno-associated virus vectors.

Adeno-associated virus (AAV) vectors have a number of attractive features, including lack of cytotoxicity, ability to transduce nondividing cells, and long-term transgene expression. We investigated whether rat renal cells could be efficiently transduced with AAV vectors. Rat glomerular mesangial cells were transduced with AAV-lacZ vector containing beta-galactosidase gene in vitro, and the expression of beta-galactosidase was evaluated by X-gal staining and ELISA. For ex vivo experiments, sections of rat kidneys were incubated with AAV-lacZ, and then evaluated by X-gal histochemical staining. The level of beta-galactosidase expression in cultured rat mesangial cells increased in a dose-dependent manner (ranging from 1 x 10(5) to 5 x 10(6) particles/cell). When transduced with 5 x 10(6) vector particles/cell of AAV-lacZ, about 50% of mesangial cells were stained positively with X-gal, and the level of beta-galactosidase expression reached 9.9 +/- 1.5 ng/mg protein. Expression was detectable during the culture period for at least 7 days. X-gal histochemical examination of the ex vivo transduced renal tissue revealed tubular cell and interstitial tissue staining. However, gene transfer was not clearly observed in glomeruli. These findings suggest that AAV vectors have the potential for gene therapy of renal diseases.

Animals↗
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Group evaluation of programs unites educators.

The Northwestern Washington Chapter of the American Society for Health Manpower Education and Training focused on alternative methods of educational programming to create a forum for planned constructive sharing of ideas and problem solving. This project served not only to enhance the existing program, which consists of a quarterly business meeting and educational events, but also to strengthen the cohesiveness of the membership

Evaluation Studies as Topic↗
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The troubled employee. Dangerous and violent behavior.

The events precipitating violent behavior, demographic characteristics of violent people, sources of stress, cognitive and affective factors, historical and current contexts of violence, the means of violence and the victims are all addressed.

Dangerous Behavior↗