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Biomedical subjects

J Mitra

Publications and source records attributed to J Mitra.

104 records · Page 6Linked to original sources
PubMedDate not supplied

Chinese hamster ovary chromosomes and antigens in tobacco/hamster heterokaryons.

Tobacco GGLL/hamster CHO hamster heterokaryon frequency following cell fusion has been raised to 4-10 percent with a modified PEG procedure. In heterokaryons, GGLL nuclei become activated, adhere to and appear to fuse with CHO nucleo. Metaphase CHO chromosomes can be identified 24 hours after fusion and immunofluorescent CHO cell surface antigens diffuse into the plant cell membrane. Diffusion coefficients, D, are estimated to be between 0.6 X 10(-11) and 1.6 X 10(-11) cm2sec-1.

Animals↗
PubMedDate not supplied

Spleen-shape as a genetic marker in X/Gf mice.

This study revealed that the triangular shaped spleen of X/Gf mice constitutes a genetically dominant characteristic over normal spleen shape of C3H mice. The agouti coat color of C3H mice is dominant over the white color of the X/Gf strain. The mode of transmission of triangular shape of the spleen in X/Gf mice, as well as their white coat color indicates Mendelian autosomal inheritance involving two independent genes. Although the pedigree lends support to this inference, we are aware that the small number of F2 offspring available for this study limits the conclusions to be drawn from the segregation ratio and statistical evaluation.

Animals↗
PubMedDate not supplied

Regulation of gene expression and cell growth in vivo by tetracycline using the hollow fiber assay.

The hollow fiber assay presents a potentially unique tool to study the effects of regulated gene expression in cell lines that do not form tumors in vivo. The hollow fibers allow small molecules to pass freely through while keeping the cells within the fibers and segregated from host cells. OSp16.1 cells, derived from the U24 clone of the U2-OS osteogenic sarcoma tumor line, express the p16INK4a tumor suppressor under the regulation of tetracycline (tet) (Mitra J et al. Mol Cell Bio 19:3916, 1999). The in vitro induction of p16 in the OSp16.1 cell line is regulated by tet. The hollow fiber assay was used to determine whether the regulation of the p16 gene could be achieved in vivo, since these cells did not grow in the xenograft model. There were no differences in the in vivo growth pattern of U24 cells loaded into the hollow fibers with and without tet: 807% and 839% net growth, respectively. OSp16.1 cells in fibers in mice receiving 3.33 mg/kg/day tet had a 644% net growth after 21 days. There was a 194% net growth without tet. Immunoblotting of extracts prepared from the hollow fibers confirmed that p16 was induced in the absence of tet. These data demonstrate this assay is a useful tool for studying the effects of regulated gene expression in vivo.

Animals↗
PubMedDate not supplied

Utilisation pattern of blood in a teaching hospital of Kolkata.

An annual utilisation of blood transfusion services at several depts. of R.G. Kar Medical College and Hospital, Kolkata were assessed among a sample of 3122 patients selected by Systematic Random Sampling Technique from the register made available in the blood bank there. Record analysis revealed that overall 79.4% of supplied blood units were actually transfused, wastage of 21.6%. Wastage was maximum in the Department of Gynae & Obstetrics (33.11%) and Surgery (32.87%). Only single unit of blood was requisitioned and transfused for 5.44% & 30.90% of the patients respectively. Most common indications of blood transfusion were for surgical cases (37.92%), followed by anaemia (34.80%) and haemorrhage (26.92%). The use of blood and blood products merit attention, appraisal and instructional guiding accordingly.

Anemia↗
PubMedDate not supplied

Length of stay of in-patients of a gynaecological ward in a sub-divisional hospital in West Bengal.

Bed utilisation efficiency and hospital resource utilisation are of prime importance to remove the "Hospital Bottlenecks" which in turn reduces length of stay of in-patients. The present study on in-patient system in Gynaecology Ward of a non-teaching hospital revealed that a considerable number of bed days were unnecessarily lost during various activities related to investigation, operation and post-operative stay. Overall length of stay found in this study (14.02 days) is considerably higher than Indian optimum. Average time-lags found between admission to pre-operative investigation and post-operative stay were 3.25 days and 10.22 days respectively.

Bed Occupancy↗
PubMedDate not supplied

Coverage evaluation surveys amongst children in some blocks of West Bengal.

Immunisation coverage evaluation was carried out in 9 Blocks of West Bengal amongst 12-23 months old children. Fully immunised status of 5 blocks were under 16%. Poor measles vaccine coverage thought to be the reason. Other vaccine coverage was more than 60%, in most of the blocks excepting Hilly Balurghat and Tamluk. "Child ill-not brought" was the important reason for immunisation failure in most of the blocks.

Cluster Analysis↗
PubMedDate not supplied

Measuring the interactions of the determinants of health.

Considering environmental sanitation and health care services as most important determinants of health, this study was undertaken to know their impacts in an urban slum area of Calcutta. The families were studied in-depth and detail in relation to their health status in the form of morbidities and utilisation of preventive and promotive services. The health service impact was mainly assessed through comparative study between these who availed the services and to these who did not. Similarly, environmental sanitation services impact on health was measured through comparing the slum group with those who lived in Pucca houses. The criteria of priority decision on determinants was attempted through analysis of cost of these services and comparing their beneficial effects on health.

Adolescent↗
PubMedDate not supplied

Drug consumption pattern in low socio-economic group in an urban community.

117 families, having a total population of 558 were studied regarding drug consumption pattern. It was found that the average no. of episodes per person was 0.99 of which drugs were consumed for 72% of episodes. Significant difference was observed in consumption of drugs between males and females. Allopathic medicines were consumed for 93.3% of the episodes and the major source (53.8%) of medical care was from private practitioners. It was seen that the number of drugs consumed per episode of sickness rose as the per capita income increased. Vitamins and Minerals (22.3%) were the main group of drugs consumed. Drugs acting on the alimentary system (17.5%), Respiratory System (14.7%), Antimicrobials (14.6%) and Analgesics (10.8%) contributed to 80% of drug intake.

Adolescent↗
PubMedDate not supplied

Epidemiology of group-A streptococcal infection amongst children of different ethnic groups in Darjeeling.

The carrier rate of Group A Streptococcus (GAS) was studied amongst 932 children from 1+ upto 12 years of age in three major racial groups in the foothills of the Darjeeling district of West Bengal. It was altogether 13 per cent and was found to be evenly distributed in the three ethnic groups as Gurkhas: 11 per cent, Rajbanshis: 15 per cent and Cosmopolitans: 13 per cent, but the incidence of Rheumatic fever and/or Rheumatic heart diseases were unnoticed among the Gurkha children who also had significant low ASO titres.

Carrier State↗
PubMedDate not supplied

An assessment of missed opportunities for immunization in children and pregnant women attending different health facilities of a state hospital.

The present study carried out in Calcutta National Medical College, Calcutta, has revealed problem of missed opportunities of immunisation in children and pregnant women and factors contributing to the problem. The percentage of missed opportunity in children and pregnant woman are 30% and 7% respectively. The major cause in case of children were that all the vaccines are not administered in all the days of week (71.05%) and in pregnant woman were wrong ideas about contraindication (42.86%) and indifferent attitude of the health worker (42.86%). All the missed opportunities were attributed to the health care providers.

Attitude of Health Personnel↗