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Biomedical subjects

J Malec

Publications and source records attributed to J Malec.

At least 73 records · Page 4Linked to original sources
PubMed1977

Regulation of growth and DNA synthesis in mouse lymphoma L5178Y cells under conditions of nutrient depletion.

The effect of cultivation of L5178Y lymphoblasts to high density and in two kinds of nutrient-depleted medium on their growth was observed. It was found that in cell population grown to stationary phase the mitotic index and labeled thymidine incorporation dropped about 3 times in comparison with exponential control. Upon dilution with fresh medium the cells resume traverse the life cycle in partially synchronized manner. Cultivation of the cells in medium with 0-5% serum induced very rapid loss of their viability. Cultivation of the cells in medium without asparagine and with dialyzed serum caused significant slowing of their growth-rate and upon dilution with complete medium the cells entered the exponential rate of division in an asynchronous manner.

Blood↗
PubMed1977

Comparison of hydroxyurea and excess thymidine as synchronizing agents in cultures of L5178Y mouse lymphoma cells.

Comparison of double hydroxyurea and hydroxyurea-excess thymidine blockades as synchronizing procedures in cultured L5178Y cells revealed that hydroxyurea mimics the effect of thymidine as regards the degree of the resulting synchrony evaluated by the mitotic index and 3H-thymidine incorporating activity. However, the peaks of the mitotic and 3H-incorporating activity appear later after thymidine than after hydroxyurea blockade and the initial maximum incorporation of 3H-deoxycytidine into DNA was about twice higher in thymidine- than in hydroxyurea-treated cells. On the other hand, the timing of 14C-formate incorporation into purine DNA bases was closely similar in both cell populations.

Animals↗
PubMed1971

[Nuclear envelope].

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Adenosine Triphosphatases↗