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Biomedical subjects

J M Sherman

Publications and source records attributed to J M Sherman.

At least 55 records · Page 3Linked to original sources
PubMed1981

Mucous glycoproteins from cat tracheal goblet cells and mucous glands separated with EDTA.

Respiratory tract mucous glycoproteins (MGP) are secreted by goblet cells in the surface epithelium and by submucosal glands. The MGP in airway secretions collected in vivo and from airway explants in vitro represent an admixture of secretions from these sources. Incubation of cat tracheal explants in 20 mM EDTA allowed physical separation of the surface epithelium from the underlying tissues and the culture of each isolated secretory element. Separated tissues appeared morphologically intact and continued to secrete MGP. The MGP arising from the surface epithelium had more sialic acid relative to galactose and N-acetylglucosamine and appeared to be less highly sulfated, as determined by DEAE cellulose chromatography, than MGP from submucosal glands. The EDTA-mediated separation of the airway secretory elements may allow further investigation into specific secretory functions of these two epithelial elements.

Animals↗
PubMed1978

The psychiatric screening clinic at a VA hospital: making it work.

A psychiatric crisis and screening clinic in a general medical and surgical hospital has expanded psychiatric treatment available to general medical patients and has eliminated overcrowding on the psychiatry service. The authors describe admissions procedures and problems that existed before the clinic was developed and ways clinic staff found to deal with the bureaucracy, which include promoting personal responsibility for patient care. The clinic has met its initial goal of decreasing the amount of time patients have to wait for their first contact with professional staff. Feedback from wards indicates that staff have been successful in making appropriate dispositions.

Attitude of Health Personnel↗
PubMed1976

alpha-Adrenergic, beta-adrenergic and cholinergic mechanisms for amylase secretion by rat parotid gland in vitro.

1. Rat parotid gland slices, incubated in a balanced, buffered salt solution, were found to be physiologically stable for up to 2 hr with respect to O2 consumption, water content, extracellular space and cation content. 2. The slices could be stimulated to secrete amylase by activation of alpha-adrenergic, beta-adrenergic or muscarinic cholinergic receptors. 3. The secretion elicited through all three receptors appeared to involve exocytosis as revealed by electron microscopy. 4. The beta-agonist, isoprenaline, increased tissue content of cyclic adenosine 3',5'-monophosphate (cyclic AMP); alpha-adrenergic and cholinergic agents had no effect on the level of this cyclic nucleotide. 5. Secretion via cholinergic or alpha-adrenergic mechanisms required extra-cellular calcium; the beta-adrenergic mechanism did not. 6. It was concluded that stimulation of rat parotid cells activates distinctly separate pathways leading ultimately to exocytosis, one pathway involving cyclic AMP, and the other, external Ca2+ ion.

Amylases↗