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Biomedical subjects

J Lu

Publications and source records attributed to J Lu.

At least 685 records · Page 38Linked to original sources
PubMed1987

Genetic resistance to mammary tumorigenesis in a mouse strain with high murine mammary tumor virus expression.

Although II-TES mice release large amounts of murine mammary tumor virus (MMTV) in milk, they are resistant to mammary tumorigenesis. High mammary tumor incidence was observed in (BALB/ca X II-TES)F1 and (C57BL/6N X II-TES)F1, whereas no mammary tumors developed in BALB/ca X OZ-F)F1. Mammary tumors developed in 68% of (OZ-F X (OZ-F X II-TES)F1 and 45% of (II-TES X (OZ-F X II-TES)F1). These results suggest that the II-TES mouse carries a recessive gene for mammary tumor resistance which does not inhibit MMTV release, and two independent dominant mammary tumor promoting genes which are inhibited by the resistant gene.

Animals↗
PubMed1986

Analysis of surface deposits on intrauterine contraceptive devices.

Surface deposits on stainless steel contraceptive devices removed from the uterus after varying periods of insertion were examined and analyzed by means of scanning electron microscopy (SEM), X-ray energy dispersive analysis (XEDA), Augar electron spectroscopy (AES), X-ray photoelectron spectroscopy (XPS), transmission electron microscopy (TEM) and atomic absorption spectrophotometry for microquantitative analysis. The data obtained were compared with the data for copper-bearing IUDs (TCu200) obtained by the same method. The wire coils of stainless steel IUDs which had been in situ for 5 years showed no obvious changes, but copper wire coils showed varying degrees of erosion after the same period of time. Analysis clearly showed that the wire coils of both types of IUD were encrusted with deposits. Deposition began earlier on copper-bearing IUDs than on stainless steel IUDs (27 days and 3 months after insertion, respectively). This process began earlier than has previously been reported in the literature. The amount of deposit increased with duration of insertion, but there were large individual variations. The microanalysis showed that the principal component of the deposit on both types of IUD was calcium, which was present in the crystal form of calcium carbonate. The substances attaching to the crystals were similar to the nitrogenous organic compounds. We suggest that the mechanism regulating the deposited formation may be similar to that of calculus. The influence of these deposits on complications relating to IUD insertion, such as uterine bleeding and accidental pregnancy, is briefly discussed in this article. The subject should be studied in greater detail.

Calcium Carbonate↗
PubMed1983

Oxygen quenching of sensitized terbium luminescence in complexes of terbium with small organic ligands and proteins.

Oxygen does not quench the luminescence of either free Tb or of Tb bound to dipicolinate. However, sensitized Tb luminescence in complexes of that ion with elastase, thermolysin, and alpha-amylase is quenched by oxygen at rates that far exceed that with which the intrinsic fluorescence of the proteins is quenched. We infer that this more rapid quenching of Tb luminescence indicates a major role for energy transfer from tryptophan moieties in a triplet excited state.

Amylases↗
PubMed1982

Cloning of a yeast gene coding for arginine-specific carbamoyl-phosphate synthetase.

Several recombinant plasmids containing cpaII, the gene that encodes the large subunit of yeast arginine-specific carbamoyl-phosphate synthetase [carbamoyl-phosphate synthetase (glutamine-hydrolyzing), carbon-dioxide: L-glutamine amido-ligase (ADP-forming, carbamate-phosphorylating), EC 6.3.3.5], have been isolated. The plasmids were selected by transformation of a yeast strain with a mutation in the structural gene of the large subunit of carbamoyl-phosphate synthetase. By using a recombinant pool with inserts of yeast nuclear DNA of 5-20 kilobase pairs, we obtained 13 transformants. Of five transformants studied, three have been found to have stable plasmid inserts. These plasmids could be amplified in Escherichia coli and transferred back into the yeast carbamoyl-phosphate synthetase-deficient strains with concomitant complementation of the nuclear mutation. Plasmids pJL2/T1 and pJL2/T5 contain identical nuclear DNA inserts of 5.9 kilobase pairs. Although the insert of plasmid pJL2/T3 is also 5.9 kilobase pairs long, the sequence overlap with pJL2/T1 and pJL2/T5 is only 4.5 kilobase pairs long. The T3 insert has an orientation in the vector opposite to that of the T1 and T5 inserts. The recombinant plasmids with the yeast cpaII gene fail to cross-hybridize with a cloned fragment of E. coli DNA containing the carA and carB genes for the bacterial carbamoyl-phosphate synthetase.

Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzin↗
PubMed1982

Diet and vitamin nutrition of the high esophageal cancer risk population in Linxian, China.

In order to examine the nutritional status of the population of Linxian (in Henan, China) known to be at high risk for esophageal cancer (EC), we analyzed blood samples and conducted 3-day dietary surveys on 3 groups of normal adults, age 40-50, from Henan province. Two groups were from Linxian, where the EC mortality rate is about 138/100,000 (Group C, consisting of volunteers with a family history of EC, and Group NC, consisting of those who did not have a family history of EC). The third group, Group F, was from Fanxian, where the EC mortality rate is about 24/100,000. The average plasma retinol level was found to be about 0.27 micrograms/ml in all 3 groups; about 13%-20% of the individuals had a level less than 0.2 micrograms/ml. The average plasma carotene level of all 3 groups appeared to be adequate, ranging from 0.5-1.0 micrograms/ml. About 23% of the subjects in both Groups C and NC had low (less than 2 micrograms/ml) plasma ascorbate levels; in Group F, 16% had low ascorbate levels. Less than 12% of the individuals in each of the 3 groups had erythrocyte glutathione reductase activation coefficients less than 1.2, indicating a widely occurring riboflavin deficiency. Consumption of alcoholic beverages was not found to be a contributing factor to EC. The results of the present study reveal a deficiency of vitamins in the study populations, but do not indicate that the average nutritional status of the volunteers in Group C was worse than that of Groups NC and F with regard to vitamin A, ascorbate, and riboflavin.

Adult↗
PubMed1982

"Medical oophorectomy" using a long-acting GNRH agonist--a possible new approach to the treatment of endometriosis.

Five women with endometriosis were given a daily dose of a potent long-acting GnRH agonist, D-Trp6-Pro9-Net-LHRH (GnRH-A) for 28 days in an attempt to suppress ovarian estrogen secretion. The mean level of estradiol (E2) during sampling over 24 hours decreased (P less than 0.01) from 62 +/- 11 to 10 +/- 1 pg/ml at the end of treatment. Mean concentrations of androstenedione, testosterone, estrone and E2 on day 28 of therapy were similar to those measured in oophorectomized women. The level of FSH was decreased (P less than 0.001) during GnRH-a, whereas that of LH was significantly (P less than 0.001) increased, suggesting differing intracellular control mechanisms for release of the two gonadotropins. Desensitization of the pituitary was demonstrated at the end of treatment by a complete lack of acute response of FSH or LH to the daily dose of GnRH-a. "Medical oophorectomy" provides a new approach to the treatment of endometriosis.

Endometriosis↗
PubMed1982

Hepatic damage in experimental and clinical paragonimiasis.

The hepatic damage induced by Paragonimus skrjabini, and the migration route of this species and that of P. westermani, were investigated in a series of animal experiments and in 34 clinical and four autopsy cases of paragonimiasis. The major symptoms and signs of an unique case reported from Sichuan Province included hepatic tenderness, disturbance of hepatic functions, remittent fever, and cough with bloody sputum. Laparotomy revealed marked adhesions surrounding the liver, spleen and duodenum. Diagnosis was made by finding P. skrjabini in a bulla on the abdominal wall about 5 months after the onset of illness. After specific treatment with bithional, body temperature and liver functions returned to normal. Twenty-three of 33 cases independently studied from Fujian Province also showed similar hepatic function disturbance. Autopsy cases showed hepatic chronic eosinophilic abscesses with Charcot-Leyden crystals. Experimentally, P. skrjabini infection caused hepatic damage in all laboratory animals used. The main pathologic changes were initially acute eosinophilic abscesses in the liver, worms being found sometimes in the necrotic cavities of the abscesses; hepatomagaly also occurred. Some cysts containing mature worms were found in the liver of dogs 3-9 months after infection. Numerous eggs were deposited in the cyst walls. The migration route of P. skrjabini was studied in young dogs. Worms appeared in the liver initially on day 3 and reached their highest level on day 14. P. westermani also migrated into liver, but their numbers were less than those of P. skrjabini.

Acute Disease↗
PubMedDate not supplied

Two applications of wavelets and related techniques in medical imaging.

We present two applications of wavelet and related techniques to problems arising in medical imaging. Both make considerable use of the edge detection and classification properties of wavelet-type representations. First we describe simple and effective techniques for image denoising and contrast enhancement based on the multiscale edge representation of images. These techniques are sufficiently flexible to successfully address the varying requirements posed by several different medical imaging modalities in common use today. Experimental results are presented to illustrate the application of these techniques to various types of medical images. Next we describe adapted waveform encoding, a technique for magnetic resonance imaging. One advantage of this technique is that it can be used to efficiently encode edge features of the object being imaged. This has a particular diagnostic application in tracking heart wall thickness during the cardiac cycle, which we present along with some experimental results along this line. We also present an analysis of the signal-to-noise ratios of images formed with this technique, as this is a factor of paramount importance in MRI. The fact that wavelet schemes tend to concentrate energy near edge features makes the result rather different than that found in standard Fourier based approaches. We indicate an exciting potential application of our technique: reducing spectral leakage in phosphorus spectroscopy.

Algorithms↗
PubMedDate not supplied

Nanoscale fluorescent sensors for intracellular analysis.

There is a growing interest in the development of submicron optochemical sensing devices. Miniaturization of sensors to nano-dimensions decreases their typical response time down to the millisecond time scale. Their penetration volume is reduced to a few cubic micrometers and they exhibit a spatial resolution at the nanometer scale. In this review the fabrication of submicron optical fiber fluorescent sensors and particle-based fluorescent nanosensors is described. The functional characteristics of these exciting miniaturized fluorescent sensors and their applications for quantitative measurement of intracellular analytes are demonstrated.

Animals↗
PubMedDate not supplied

Albendazole chemotherapy for human cystic and alveolar echinococcosis in north-western China.

Human echinococcosis is highly endemic in north-western China; the main treatment is by surgery. In this paper, we report the results of chemotherapy with albendazole (ABZ), 15-20 mg/kg/d orally, for 30 d with intervals of 10 d between treatments for 3-6 courses. For multi-organ cystic echinococcosis (CE) and alveolar echinococcosis (AE), patients were given 12-18 courses of ABZ. Patients were divided into 4 groups: (i) ABZ surgery group, albendazole with surgery for 21 CE cases: (ii) non-ABZ surgery group, 80 CE cases treated by surgery alone; (iii) ABZ CE group, albendazole treatment alone in 58 CE cases, and (iv) ABZ AE group, 14 AE patients treated by albendazole and surgical intervention and 5 AE patients treated by albendazole alone. Twenty-seven of 34 (79.4%) cysts in group (i) patients showed increased necrotic changes and decreased viability of the cysts compared to group (ii). However, 10 of 84 (11.9%) cysts in group (ii) patients showed spontaneous evidence of necrosis at surgery. In group (iii), ABZ treatment alone was successful in 14 (24.1%), resulted in improvement in 29 (50%) and had no effect in 15 (25.9%) patients. Seven cases in group (iv) improved, with diminished size of lesions which were non-viable. The remaining 7 cases in group (iv) showed evidence of cyst viability at surgery; 2 could not be saved after a further 15 courses of albendazole. Of the five AE patients in group (iv) who received only ABZ, one improved, 2 stabilized, one deteriorated and one died. Albendazole chemotherapy, while not completely effective, has an important role in treatment of both cystic and alveolar echinococcosis.

Administration, Oral↗
PubMedDate not supplied

Vulnerability of long thoracic nerve: an anatomic study.

The anatomic course of 40 long thoracic nerves was studied in relation to anatomic landmarks and reference lines, that is, the axillary lines and first 2 ribs. After its supraclavicular course, the nerve passes beneath the clavicle within the axillary sheath and then emerges from the axillary sheath. As it passes inferiorly and posteriorly from the point of emergence to the posterior angle of the second rib (that is, the attachments of serratus anterior muscle), it makes a posterior angle of 30.7 degrees +/- 4.3 degrees on average, relative to the anterior axillary line. It then continues to descend inferiorly between the middle and posterior axillary lines. When the arm is raised, the axillary neurovascular bundle moves superiorly with the movements of the arm. The long thoracic nerve is angulated and stretched at the point it passes out of the axillary sheath.

Adult↗
PubMedDate not supplied

A model of a segmental oscillator in the leech heartbeat neuronal network.

We modeled a segmental oscillator of the timing network that paces the heartbeat of the leech. This model represents a network of six heart interneurons that comprise the basic rhythm-generating network within a single ganglion. This model builds on a previous two cell model (Nadim et al., 1995) by incorporating modifications of intrinsic and synaptic currents based on the results of a realistic waveform voltage-clamp study (Olsen and Calabrese, 1996). Due to these modifications, the new model behaves more similarly to the biological system than the previous model. For example, the slow-wave oscillation of membrane potential that underlies bursting is similar in form and amplitude to that of the biological system. Furthermore, the new model with its expanded architecture demonstrates how coordinating interneurons contribute to the oscillations within a single ganglion, in addition to their role of intersegmental coordination.

Animals↗
PubMedDate not supplied

Chromosomal location of lectin genes indicates they are not the basis of Rhizobium strain specificity mutations identified in pea (Pisum sativum L.).

A lectin gene family is located on linkage group 7 in pea. The lectin genes are arranged as a cluster, with no recombination observed within the multigene family. A lectin-like cDNA clone, pEA207, and eight DNA fragments generated by random priming also were mapped in the region of the lectin genes. None of the known pea mutants altering Rhizobium leguminosarum strain specificity map to this region of the genome, and therefore their altered specificities appear not to be directly produced by mutations in the lectin genes.

Base Sequence↗