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Biomedical subjects

J Leonardelli

Publications and source records attributed to J Leonardelli.

At least 55 records · Page 3Linked to original sources
PubMedDate not supplied

Presence of neuropeptide--Y and its C-terminal flanking peptide immuno-reactivity in the seminiferous tubules of human testis.

The presence of neuropeptide Y (NPY) and its C-terminal flanking peptide (C-PON) was described by immunohistochemistry in human testes. The immunopositive material was visualized in the spermatogenic elements of the seminiferous tubules. More NPY occurred in the younger testis and more C-PON in the older ones. NPY positive material was present mainly in the spermatogonia, and in the primary spermatocytes, where C-PON also occurred. The megalospermatocytes, present in aged testis, showed C-PON immunoreactivity. Both NPY and C-PON were present in granular form in the perinuclear zone of the cells. No positive material was detected in the Sertoli cells or in the Leydig cells. It is possible that NPY and its precursor are synthetized within the testis and might play a role in the paracrine and/or autocrine regulation of spermatogenesis.

Adult↗
PubMedDate not supplied

Immunohistochemical detection of prolactin and its receptors in human testis.

Prolactin (PRL) and PRL receptors were localized by light microscopic immunohistochemistry in the human testes on interstitial Leydig-like cells. Similar stainings were obtained with both monoclonal antihuman PRL antibodies and polyclonal antibodies raised against rabbit mammary gland PRL receptors. Moreover, the detection of PRL binding sites by radio-receptor assay on the same samples gave a confirmation of these results. This suggests that PRL might act directly on Leydig cells in the man, in the same way as in the rodent.

Aged↗
PubMedDate not supplied

[Hypothalamic LH-RH neuronal modifications after prolactine intraventricular infusion in the guinea pig (author's transl)].

Ovine prolactine injected at doses of either 31,25 to 500 microgram by lateral intraventricular route in male guinea pigs, affects LH-RH hypothalamic neurons within 2 to 24 hours. After this treatment there is an increase of LH-RH immunoreactive material in eminential terminals and in numerous small granules of hypothalamic neuronal cell bodies. This accumulation of immunoactive LH-RH in pericaryon and in terminals can be interpreted as stimulation of LH-RH synthesis. Preliminary results of radioimmunoassayed plasmatic LH level, 2 h after PRL infusion, are in agreement with this hypothesis. The probability that short feed-back by PRL or PIF secretion would simultaneously be able to check LH-RH secretion is discussed.

Animals↗