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Biomedical subjects

J Koch

Publications and source records attributed to J Koch.

At least 271 records · Page 15Linked to original sources
PubMed1977

Urinary kallikrein in rats bred for susceptibility and resistance to the hypertensive effect of salt and in New Zealand genetically hypertensive rats.

Urinary kallikrein excretion was studied in rats bred for susceptibility and resistance to the hypertensive effect of salt. The rats were on a regular rat chow diet (0.45% sodium content) and tap water and were not hypertensive at the time of the study. Urinary kallikrein excretion, measured by kinin radioimmunoassay, was 10 to 20 times lower in the susceptible rats than in the resistant rats (4.39 +/- 1.61 microgram/24 hours and 56.4 +/- 5.8 microgram/24 hours, respectively; P less than 0.001). Urinary kallikrein excretion was also measured in New Zealand genetically hypertensive rats and in normotensive Wistar-Otago rats (controls). Kallikrein was found to be significantly lower in the genetically hypertensive rats than in the controls (49.1 +/- 6.2 microgram/24 hours and 76.8 +/- 6.9 microgram/24 hours, respectively); however, when expressed per 100 g of body weight, there was no significant difference. In conclusion, although urinary kallikrein excretion per rat was decreased in the genetically hypertensive rats when compared with the controls, this difference could be caused by the lower body weight of the genetically hypertensive rats. Urinary kallikrein excretion, when expressed per 100 g of body weight, is significantly lower in susceptible than in resistant rats. This could be a consequence of a genetic defect that may play a role in the development of hypertension, perhaps through alteration of renal function.

Animals↗
PubMed1976

Nuclease S1 cleavage and the primary structure of mitochondrial DNA.

The single-strand-specific nuclease S1 from Aspergillus oryzae rapidly converts superhelical mitochondrial DNA (African Green Monkey cells, Vero ATCC; CCL 81) into nicked circular DNA. These nicked mitochondrial DNA molecules contain two nicks, one in each strand. The phosphodiester backbones are cleaved during this reaction at or near sites that are alkali-labile. In a second slow reaction the circular mitochondrial DNA is converted into a linear duplex DNA. Permutation tests indicate that this linear DNA represents a nonpermutated collection of DNA molecules. These results suggest that two of the alkai-labile sites in the phosphodiester backbones of the mitochondrial chromosome are closely spaced on opposite strands and at specific positions.

Aspergillus oryzae↗
PubMed1976

[Urethro-cysto-colpo-rectography, a radiological method for the diagnosis of insufficiency of the pelvic floor (author's transl)].

Contrast examination of pelvic organs was carried out in 200 female patients with insufficiency of the pelvic floor. The topography of the organs in the true pelvis and their functional relation was recorded on full-size film and 70 mm. roll film. Radiological examination provides more accurate indications for plastic surgery of the pelvic floor, influences the operative procedures and permits better evaluation of operative results.

Contrast Media↗
PubMed1976

J blood group active lipoproteins extracted from bovine erythrocytes.

Ghosts of J-positive bovine erythrocytes were frozen overnight, thawed the next day, washed with ion-free water, and then extracted with a water-n-pentanol mixture. After centrifugation, the aqueous phase contained a great deal of the membrane proteins and lipids in a soluble form along with the J blood-group activity. After preparative ultracentrifugation, about 2/3 of the J activity were recovered in the high-density lipoprotein fraction, while the low-density lipoprotein fraction contained 1/3 J activity. This result is consistent with out finding that on treatment of the stroma lipoproteins with dextran sulphate, about 2/3 of J activity were recovered in the supernatant, 1/3 in the precipitate. These fractions obtained by dextran sulphate treatment were characterized by protein and lipid assay.

Animals↗
PubMed1976

[Representation of gynaecological fistulas by wick urethrocystocolporectography (author's transl)].

In connection with gynaecological diseases and/or their therapy, genital fistulas constitute a grave complication. Accurate representation of these fistulas in conjunction with the adjacent hollow organs is of curcial importance for their successful removal. A modified kind of wick urethrocystocolporectography, which is a method that has proved efficient in cases of a dislocation in the female genital tract and also in cases of insufficiency of locking of the urinary bladder, provides these preconditions for an efficient therapy. That is, through the difference in the course of motion during holding and pressing in comparison with rest there are detected not only any valve mechanisms present but also dislocations, if any. Moreover, the method, easy to apply as it is and involving only little strain, provides an advantage which cannot be overlooked.

Colon, Sigmoid↗
PubMed1976

[Participation of spouses of neurotic inpatients on the neurotic's views pertaining to their superiors and place of employment].

In a total of 72 sessions, 40 neurotic patients and their partners in marriage, who were interrogated separately, expressed their views of the superiors and employers of the patients. Statistical analyses showed that there was a great similarity between the comments made by the patients and their spouses. The basic psychodynamic process of identification was considered responsible for this. Six modes of identification with the neurotic partner were described. Also discussed by the author are the value and limits of "objective anamnesis". The need for including the entire family in the program of therapy is pointed out.

Adjustment Disorders↗
PubMed1975

Tertiary structure and function of closed-circular mitochondrial DNA and its transcription in vivo.

The conformation of the closed circular mitochondrial DNA in cultured human cells is changed by the addition of berenil to the culture medium in such a way that the 34-S mitochondrial DNA is converted into a 29-S DNA and finally into a 24-S DNA. The superhelix density of the covalently closed 29-S mitochondrial DNA is sigma O = -1.5 times 10(-2) (turns/10 base pairs) and consequently is intermediate between the superhelix density of the 34-S DNA (sigma0 = -2.8 times 10(-2)) and that of the closed circular 24-S DNA (sigmao appromiately 0). Removal of the drug reverses the transition, covalently closed circular 34-S DNA to covalently closed circular 29-S DNA to covalently closed circular 24-S DNA. The covalently closed circular 24-S mitochondrial DNA is not replicated. Transcription of this DNA is also inhibited as indicated by the fact that no synthesis of the mitochondrial ribosomal RNAs occurs as long as the mitochondrial DNA is in this conformation. The covalently closed circular 29-S mitochondrial DNA is replicated but not transcribed in vivo.

Anti-Bacterial Agents↗
PubMed1975

Replicating mitochondrial DNA from human, monkey and mouse cells. The superhelix density of the closed-circular parental strands.

Pulse-labeled replicating mitochondrial DNA molecules of three different cell lines, mouse NCTC 929 (ATCC;CCL 1), monkey Vero (ATCC;CCL 81) and human WISH (ATCC;CCL 25) were classified according to their sedimentation velocity in neutral sucrose gradients. Replicating molecules were isolated and and the newly synthesized strands were dissociated from the parental DNA strands. This procedure yielded parental closed circular duplex DNA with a higher sedimentation velocity in neutral sucrose gradients and higher absolute values of superhelix density in comparison with values for mature unreplicating supercoiled mitochondrial DNA. Replicating molecules with these properties, i.e. higher absolute superhelix densities of the parental strands, comprise 2--4% of the total mass of the mitochondrial DNA in all three cell lines. In addition, mouse cells contain a large proportion of D-loop mitochondrial DNA molecules (28 S). Upon dissociation of the 8-S single-stranded initiation sequence from the parental strands, these molecules yielded parental supercoiled duplex DNA with a superhelix density similar to that of mature supercoiled mitochondrial DNA. D-loop mitochondrial DNA represents about 30% of the total mass of the mitochondrial DNA in mouse cells. Therefore, two populations of replicating mitochondrial DNA can be distinguished in mouse cells. One of these accumulates as D-loop mitochondrial DNA with constant topological winding, while the other replicates in a manner similar to that of Vero and Wish cells under progressively decreasing topological winding of the parental strands.

Animals↗
PubMed1975

[Functional risks in the surgical therapy of urinary incontinence and prolapse (author's transl)].

In addition to the known surgical risks in the treatment of incontinence or prolapse there exists a functional risk inherent in the surgical methods, which can even cause a deterioration in the functional condition if the surgical aim is not achieved. The vaginal bladderneck sutures are only worth as much as the tissues they hold together. The surgical treatment of certain cases of prolapse can eliminate the stopcock mechanism of the urethra and lead to the manifestation of a preoperatively-existent incontinence. With the sling operations success depends, among other factors, on the degree of restraint and the correct placement of the sling. The outcome of hysterectomy is endangered by failure to recognize a coexistent prolapse or enterocoele. With Ward's manipulation, high peritonization and adequate treatment of the overextened posterior vaginal vault, the involved functional risks can be minimized. Typical examples are mentioned in the text. Particular emphasis is placed on the necessity of exact knowledge of the circumstances threatending the functional success and their recognition by means of wick-urethrocystocolporectography. For the maintenance of a good doctor-patient relationship, the patient may receive prior to surgery an understandable explanation of the limitations of the methods used in achieving a functional cure.

Aged↗
PubMed1975

[Demonstration of position variations of the female ureter in the work urethrocystocolporectogram].

With wick urethrocystocolporectography, an improved type of colpocystorectography, the form and undisturbed function of the urethra at rest, and during holding and pressing, may be optimally observed. The simultaneous picture of the female pelvic organs allows conclusions to be drawn as to the statics and dynamics of the viscera, as well as the functional strength of the pelvic floor. Illustrations show the behavior of the female urethra accompanying vertical and rotatory descensus, and prolapse.

Female↗