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Biomedical subjects

J Keller

Publications and source records attributed to J Keller.

At least 217 records · Page 12Linked to original sources
PubMed1987

[Comparative immunofluorescence study of actinic keratosis and chronic discoid lupus erythematosus].

Regarding systemic (SLE) and chronic discoid lupus erythematosus (CDLE), the diagnostic value of the lupus band test ist generally accepted. In the literature, however, there are but few obligatory criteria concerning the definition of a positive lupus band. In order to illustrate the influence of sunlight on the evolution of junctional deposits of immunoglobulins, we examplarily studied actinic keratosis (AK) as a chronic light-dependent dermatosis. The junctional deposits in AK were qualitatively and quantitatively compared with the lupus band typical for CDLE. In CDLE we mostly found more distinct band-like junctional deposits of immunoglobulins and complements. Light-dependent, non-specific junctional patterns of immunofluorescence similar to LE, therefore, require clear morphological criteria of immunohistology.

Aged↗
PubMed1987

Detection of c-rel-related transcripts in mouse hematopoietic tissues, fractionated lymphocyte populations, and cell lines.

A portion of the human cellular homolog of v-rel, the transforming gene of the leukemogenic retrovirus reticuloendotheliosis virus, strain T, was used to survey RNAs from several mouse tissues, selected lymphocyte populations, and hematopoietic cell lines for c-rel expression. Relatively high levels of a high-molecular-weight transcript were observed in peripheral B and T cells, whereas lower levels were detectable in functionally immature thymocytes. These results suggested that, unlike c-myb and c-ets, the c-rel proto-oncogene plays a role in later stages of lymphocyte differentiation.

Animals↗
PubMed1987

Bone repair inhibited by indomethacin. Effects on bone metabolism and strength of rabbit osteotomies.

We measured mineral content, maximum bending strength, and regional blood flow after tibial osteotomy fixed with a small metal plate in 38 rabbits. Half of the animals were treated with indomethacin (10 mg/kg/day) while the other half served as controls. After 2 and 6 weeks, the bone mineral content and maximum bending strength were lower in the indomethacin group when compared with the controls. Compared with the controls, the blood flow at the osteotomy site was decreased after 2 weeks and increased after 6 weeks in the indomethacin-treated animals. Inhibition of blood flow increase by indomethacin medication in the early period following osteotomy, as well as retarded bone healing, are probably caused by inhibition of the inflammatory reaction.

Animals↗
PubMed1986

[Sweet syndrome with immune complex vasculitis in a child].

We report on a rare case of Sweet's syndrome (SS) in a 7-year-old boy showing typical symptoms such as high fever, neutrophil leucocytosis in peripheral blood, elevated blood sedimentation rate, and arthralgia. The cutaneous lesions were painful erythematous, raised plaques on both limbs and the face. Histology confirmed the clinical diagnosis, including remarkable signs of cutaneous small vessel vasculitis, which was verified by direct immunofluorescence for IgM and complement (C3) in vessel walls as well as by the presence of circulating immune complexes in the blood. By means of monoclonal antibodies, T-lymphocytes and macrophages could be identified in the perivascular infiltrates in the delayed phase of SS. According to these immunological findings, we discuss the pathomechanism and the therapeutical benefit of inhibitors of cyclooxygenase.

Capillaries↗
PubMed1986

[Benign chronic bullous dermatosis in childhood with involvement of the mouth mucosa].

We report on a rare case of benign chronic bullous dermatosis of childhood (BCBDC) in a 5-year-old girl. In addition to the characteristic symptoms such as abrupt onset of the disease, tense blisters, predominant affection of the face, and later imitation of impetigo, we observed uncommonly extensive involvement of the oral mucosa. Whereas histopathology was not diagnostic, direct immunofluorescence could verify BCBDC as it showed linear deposits of IgA along the basement membrane zone. This case gives rise to the discussion of the clinical entity and nomenclature of BCBDC in relation to similar bullous diseases of childhood and adults.

Basement Membrane↗
PubMed1986

Visceral cortex lesions block conditioned taste aversions induced by morphine.

Rats with bilateral ibotenic acid or sham lesions of the visceral (agranular insular) cortex were tested for a conditioned taste aversion (CTA) to saccharin after five pairings of morphine sulphate injections (15 mg/kg IP) with consumption of a novel solution (0.1% saccharin). Lesioned animals demonstrated no evidence of the morphine-induced CTA that was seen in the sham operated animals. A third group of rats received ibotenic acid lesions but had saccharin consumption paired with saline vehicle injections. This group had the normal preference (seen in naive rats) for saccharin on testing, showing that the visceral cortex lesion had no effect on the ability of the rats to discriminate saccharin from water. In order to test if visceral cortex lesions abolish specifically the CTA induced by morphine, we ran a similar set of CTA experiments using two new novel flavours and either 15 or 75 mg/kg IP lithium chloride (LiCl) as the unconditioned stimuli. Dose dependent CTA's to the LiCl were established in all groups indicating that the visceral cortex plays no role in mediating the aversive effect of LiCl. Using the condition place preference paradigm we investigated the role of the visceral cortex in the expression of morphine's rewarding aspects. Identical place preferences were found in groups of rats with or without visceral cortex lesions suggesting that this cortical region plays no role in either the perception of morphine's rewarding effects or the association of morphine's rewarding properties with sensory stimuli. Visceral cortex lesions also had no effect on the establishment of a conditioned place aversion to a high dose of LiCl (75 mg/kg IP). Thus, visceral cortex appears critical for the establishment of a morphine-induced CTA, but is not crucial for mediating gross taste discrimination, the aversive aspects of LiCl nor the rewarding properties of morphine.

Animals↗
PubMed1986

Type III collagen aminopropeptide levels in serum of patients with progressive systemic scleroderma.

Sera from 101 patients with progressive systemic scleroderma were analyzed for circulating aminopropeptides of type III collagen using a radioimmunoassay which measures the intact and degraded forms (Fab assay). About 41% of the patients were found to have values above the normal range. A good correlation was observed between elevated levels of aminopropeptides and the degree of involvement of the skin and internal organs in the patients. Most patients (89%) with an active progression of the disease but not those in a stationary phase showed increased serum levels of aminopropeptides. Treatment with corticosteroids apparently normalized the levels of aminopropeptides. Only minor changes were observed with an antibody-based radioimmunoassay which measures primarily the intact form of the aminopropeptide.

Adult↗
PubMed1986

[Sterile eosinophilic pustulosis].

Sterile eosinophilic pustulosis (SEP) first described by Ofuji et al. in 1970 as "eosinophilic pustular folliculitis," is obviously a new entity. The majority of patients are Japanese. So far only four European cases have been reported. However, we have recently observed a 46-year-old Greek male (the fifth case in Europe) with the typical clinical features of SEP: repeated eruptions of aggregated pruritic (follicular) papulopustules (1-2 mm in diameter). The eruptions developed in slightly elevated erythematous patches localized on the extremities, face, and trunk. Histologic examination revealed intraepidermal pustules containing eosinophils and moderate dermal infiltrates with mononuclear cells and eosinophils around follicles, sebaceous glands, and vessels. In addition to the characteristic clinical and histological features, our patient showed all the typical laboratory findings previously described: negative bacterial cultures from the pustules, blood eosinophilia, increased total IgE, negative reactions to intradermal tests of recall agents. Furthermore, the rate of suppressor/cytotoxic T-cells (OKT 8-cells) in peripheral blood was significantly diminished, and mitogenic stimulation of lymphocytes in vitro was negative (decreased LTT). These laboratory data resemble the immunopathological findings in atopic diseases. On the other hand, seborrheic skin with sterile eosinophilic pustules predominantly occurring in skin areas rich in sebaceous glands seems to be another remarkable sign of SEP. The question of whether the co-incidence of atopylike immunological constellation and seborrheic skin observed in SEP has pathogenetic importance, however remains open. Apart from corticosteroids and sulfones, a favorable therapeutic effect may be obtained by inhibitors of cyclooxygenase. In our case indomethacin caused a quick and lasting therapeutical benefit.

Antibodies, Monoclonal↗
PubMed1986

Phase II evaluation of bolus gallium nitrate in lymphoproliferative disorders: a Southeastern Cancer Study Group trial.

Gallium nitrate was administered as a 700-mg/m2 iv bolus infusion over 15-30 minutes every 2 weeks to 138 patients with malignant lymphoproliferative diseases. Responses occurred in patients with well-differentiated lymphomas (five responses among eight patients), but the drug produced few responses in any other group of patients. Toxic effects were primarily gastrointestinal and reversible renal abnormalities and anemia. As a single agent, bolus gallium nitrate has little activity in lymphoproliferative diseases.

Adolescent↗
PubMed1985

Correlation functions in the analysis of visual evoked potentials.

Our "vision" laboratory has been working for several years on stimulation methods and data processing. We present here the results of an analysis of correlation functions between VEPs obtained by flashes and patterned stimulations. This study provides additional information about possible interpretations.

Evoked Potentials, Visual↗