Search PubMed⌕ Search

Biomedical subjects

J Kato

Publications and source records attributed to J Kato.

At least 559 records · Page 31Linked to original sources
PubMed1984

[Urinary excretion of N-acetyl-beta-D-glucosaminidase in patients with urological disease: with special reference to hydronephrosis].

Activity of urinary N-acetyl-beta-D-glucosaminidase (NAG) has been studied in patients with urological diseases. In most patients with benign prostatic hyperplasia, prostate cancer and bladder cancer, activity of urinary NAG was elevated (92.9%), especially in cases of indwelling catheterization or poor visualization of IVU. Urinary NAGs were also elevated for all patients with renal stones, ureter stones, uretero-cutaneous stomy or a single kidney. In hydronephrosis or hydronephrosis with hydroureter patients, urinary NAG level was low pre-operatively, but increased post-operatively. Urinary beta 2-microglobulin (BMG) showed a similar tendency in patients with hydronephrosis or hydronephrosis with hydroureter. Electronmicroscopic observations of the kidney in such patients revealed many primary and secondary lysosomes.

Acetylglucosaminidase↗
PubMed1984

[A method for determination of prostaglandins in human seminal fluid].

Human seminal fluid has been shown to contain mainly eleven different prostaglandins. Methods for quantitative determination of prostaglandin E and 19-OH prostaglandin E are described. The methods include extraction with acetone and alkali treatment. After alkali treatment prostaglandin E was transformed to prostaglandin B, and 19-OH prostaglandin E to 19-OH prostaglandin B. Prostaglandins were separated by using Lipidex column chromatography and silicic acid column chromatography. Prostaglandin B was quantitated by gas chromatography using C24 fatty acid methyl ester as internal standard. 19-OH prostaglandin B was quantitated by measuring ultraviolet photoabsorbance at 278 nm.

Alprostadil↗
PubMed1984

Decreased progestin receptors in the cerebral cortex of hypothyroid postnatal rats.

The effects of neonatal hypothyroidism were examined on the concentrations of cytosol receptors for progestin and estrogen in the cytosols from 10-day old female and male rats. The concentration of cytosol progestin- and estrogen receptors in the 10-day old female and male rats with propylthiouracil-induced hypothyroidism was determined by multiconcentration saturation analysis. Neonatal hypothyroidism markedly depressed the level of progestin receptors in the cortex but not in the hypothalamus-preoptic area (HPOA). There were no differences in the concentration of estrogen receptors in the cortex and HPOA between the control and PTU rats. These results suggest that thyroid hormone may be one of the factors affecting the development of progestin receptors in the cortex. Its significance was discussed in relation to determination of the length of sexual differentiation of the rat brain.

Animals↗
PubMed1983

On the process of cellular division in Escherichia coli: nucleotide sequence of the gene for penicillin-binding protein 3.

We determined the nucleotide sequence of a DNA fragment containing the ftsI gene coding for the penicillin-binding protein 3 (PBP-3), an indispensable enzyme for cell division of Escherichia coli. The entire ftsI gene was within the 2.8 kilobase PvuII fragment derived from the chromosomal segment on pLC26-6 (Nishimura et al. 1977). The coding region for PBP-3 was identified by comparison with the N-terminal amino acid sequence of in vitro synthesized PBP-3. The structural gene for ftsI consisted of 1,764 base-pairs coding for a 588 amino acid residue-polypeptide with a molecular weight of 63,850. PBP-3 synthesized in vitro showed a lower mobility in SDS-gel electrophoresis than that of the authentic PBP-3, suggesting that the primary translation product of the ftsI gene may be processed to yield mature PBP-3.

Bacterial Proteins↗
PubMed1983

Estrogen receptors and estrogen-inducible progestin receptors in the sexual skin of the monkey.

Estrogen-binding macromolecules were identified in sexual skin cytosol and nuclear fractions from female Japanese monkeys (Macaca fuscata fuscata), by sucrose-glycerol gradient analysis and dextran-coated charcoal adsorption technique. Furthermore, using the highly potent synthetic progestin, R5020 (17,21-dimethyl-19-nor-4,9-pregnadiene-3,20-dione; promegestone), progestin-binding macromolecules were detected in sexual skin cytosols from ovariectomized estrogen-primed monkeys. Both cytosol components sedimented at the 8S region on sucrose-glycerol gradients and had a high affinity for the respective steroids, and competition studies showed a specificity for estrogenic, or progestational compounds. The concentration of cytosol progestin receptors was markedly increased with the elevated level of nuclear estrogen receptors following estrogen priming. The above results demonstrate the existence of cytosol and nuclear estrogen receptor sites, and estrogen-induced progestin receptors in the sexual skin of the female monkeys.

Abdomen↗
PubMed1983

Further isolation of a recombinant virus (H1N2, formerly Hsw1N2) from a pig in Japan in 1980.

In September 1980, an outbreak of febrile respiratory disease was observed in a herd of sows (1-2 years of age) in Ehime Prefecture, Japan. Most of the swine showed clinical signs of disease such as depression, anorexia, fever, nasal discharge, and cough. A hemagglutinating agent was isolated from a nasal swab from one of the diseased pigs. By cross-hemagglutination-inhibition and neuraminidase-inhibition tests with antisera to influenza viruses of swine origin, the isolate was identified as an influenza A virus of the H1N2 (former designation, Hsw1N2) subtype, and designated A/swine/Ehime/1/80 (H1N2). Significant antibody rises against the surface antigens of the isolate were found in convalescent swine sera. The distribution of antibody against H1N2 virus in swine sera in Ehime Prefecture was examined. Seven (8%) of 93 sera collected after the outbreak (in 1981) showed antibodies to only H1 and N2 antigens but none of the sera before the outbreak contained such antibodies, indicating that H1N2 virus had been restricted prevalent among swine but was not wide-spread until 1981.

Animals↗
PubMed1983

Progestin receptors in female rat brain and hypophysis in the development from fetal to postnatal stages.

The fetal and postnatal development of the progestin receptors in the intact female rat brain was investigated by means of the in vitro cytosol binding and the nuclear exchange assay using 3H-R5020 ([17 alpha-methyl-3H] 17 alpha-,21-dimethyl-19-nor-4,9-pregnadiene-3,20-dione). A large 7S peak of progestin binding in the cerebral cortical cytosols was observed at days 7-14 with less evident binding in the hypothalamus-preoptic area (HPOA). The cortical cytosol receptors, first detectable at day 0, rapidly increased at days 1-7, reaching a maximum at day 10, then gradually declined thereafter. The receptors in the HPOA and other brain areas appeared at day 1, increased during the first week, then remained constant at days 10-28. Hypophysial cytosol receptors, first detectable at day 10, increased at day 28. Nuclear progestin binding was low in the HPOA and cortex of the neonates at days 1-3. Interestingly, despite the low level of serum progesterone, the cortical nuclear binding suddenly increased at days 7-10, then remained high at days 14-21. A similar, though less pronounced, pattern was seen in the HPOA. Diethylstilbestrol injection caused an increase in the cytosol binding capacities in the HPOA, but did not in the cortex. These results suggest the appearance of the brain progestin receptor system immediately after birth, and differential patterns of their postnatal development in the intact female rat. The onset of increased nuclear translocation of endogenous progestin-receptor complex may occur in the cortex, and possibly HPOA, at around days 7-10. Progestin receptors in the postnatal rat HPOA are estrogen inducible, but not in the cortex.

Animals↗