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Biomedical subjects

J Johnson

Publications and source records attributed to J Johnson.

At least 919 records · Page 51Linked to original sources

Attenuation of Eimeria necatrix and E. tenella of U.S. origin by serial embryo passage.

Two lines of Eimeria tenella (PS and FS65) and one line of E. necatrix (FS144) of U.S. origin were passaged in chicken embryos. The embryo-passaged line of E. tenella PS was significantly less pathogenic than the parent line when tested in chickens after 33 embryo passages. After 29 embryo passages, E. tenella FS65 was just as pathogenic to chickens as the parent line. A comparison of the immunogenicity of the embryo-adapted lines and the parent lines in chickens showed that embryo-passaged E. tenella PS and E. tenella FS65 were as immunogenic as parent lines. The embryo-passaged line of E. necatrix FS144 was significantly less pathogenic when tested in chickens after 30 embryo passages. Although there was also some loss of immunogenicity in the embryo-passaged line of E. necatrix, immunity to this parasite could be achieved by increasing the dose of oocysts.

Animals↗

Results of modified Bristow procedure.

A modified Bristow procedure was performed on 23 patients over a two-year period. The modification consisted of splitting the subscapularis muscle in line with its fibers and attaching the coracoid process to the neck of the scapula with a malleolar screw. The results were evaluated by questionnaire, physical examination, and radiographs. The average age of the patients was 24 years. There were 20 men and three women. The patients presented 16 left shoulders and seven right shoulders; nine were dominant. The number of previous dislocations was 12. The average follow-up period was 25 months and the average hospital stay was two days. There were no blood transfusions. Each patient returned to his or her preoperative activity level; no patient had pain in the shoulder at rest. With overhead activity, five patients had some pain and 18 had none. Of the nine patients whose surgery was on the dominant side, only two had difficulty in throwing. There were no postoperative recurrent dislocations. There was an average loss of external rotation of 13 degrees and an average loss of internal rotation of 12 degrees. Bony fusion of the coracoid to the neck of the scapula occurred in only one out of 13 shoulders. Complications were minor and resolved completely. The advantages of the procedure are good surgical exposure, minimal tissue trauma, insignificant blood loss, and fewer days in the hospital per patient. The procedure was relatively safe and generally effective.

Adolescent↗

Renal oncocytoma: preoperative diagnosis by aspiration biopsy.

The cytologic, histologic and ultrastructural features of an oncocytoma of the right kidney with the primary diagnosis by thin needle aspiration biopsy are presented. These uncommon tumors are generally considered to be of renal tubular origin and are thought to be benign. In the case reported below, two separate tumor masses were present within the diseased kidney; one of them extended to the perirenal adipose tissue and was accompanied by satellite tumor nodules. This presentation of oncocytoma, associated with focal cellular atypia, suggests that malignant behavior of at least some of these tumors may be anticipated.

Adenoma↗

Attenuation of a strain of Eimeria mivati of U.S. origin by serial embryo passage.

A strain of Eimeria mivati (FS50) isolated in Georgia was purified and serially passaged in groups of developing chicken embryos. Starch gel electrophoresis using glucose phosphate isomerase and lactate dehydrogenase showed the parasite to be similar to another strain of E. mivati isolated in the U.S. The embryo-passaged line of E. mivati (FS50) was less pathogenic than the parent line but retained its immunogenicity. This strain may be suitable for inclusion in an improved coccidiosis vaccine. The status of E. mivati and E. mitis is discussed.

Animals↗