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Biomedical subjects

J Hustin

Publications and source records attributed to J Hustin.

At least 91 records · Page 5Linked to original sources
PubMed1979

Organ culture of mammalian testis. III. Inhibin secretion.

Mouse testes were cultured for 19--20 days at either 31 or 37 degrees C with a change of medium every 4 days. After treatment with charcoal and dextran T, the recovered testis media were incubated with rat anterior pituitary cells, and secretions of follicle-stimulating hormone (FSH) and luteinizing hormone (LH) were estimated by radioimmunoassay 3 days later. FSH release was significantly lowered when pituitary cells were grown with media of testes cultured 31 degrees C compared to cultures grown with fresh medium or with media of testes cultured at 37 degrees C for more than 4 days. LH secretion was normal in one experiment and reduced in the other with the media of testes cultured at 31 degrees C. Treatment of testicular media by heat or trypsin reduced the inhibiting activity. After 8 days at 37 degrees C, both germinal and Sertoli cells were damaged in the testis cultures, while at 31 degrees germinal cells alone were destroyed, Sertoli cells remained normal. These studies suggest that (1) a substance which responds to the definition of inhibition (protein--preferentially acting on FSH) is secreted in the medium of testis culture; (2) inhibin is produced by Sertoli cells; (3) inhibin is secreted only if the temperature is inferior to 37 degrees C.

Animals↗
PubMed1979

[Incorporation of tritiated thymidine into testicular deoxyribonucleic acid of the rat. Effect of inhibin].

tritiated thymidine is incorporated into DNA of spermatogonia type B as proved by autohistoradiography when injected in vivo three hours before the sacrifice. Maximum binding and specific activity (labelled thymidine expressed in DPM per mg DNA) are obtained in pubertal rats aged 42 days and weighting 150 g. Inhibin preparation extracted from rete testis fluid (RTF3) specifically inhibits tritiated thymidine into testicular DNA. Thus, no modification of incorporation into hepatic DNA is observed and the preparation loses its inhibitory effect when denatured by heating and trypsin digestion. Tritiated thymidine incorporation into testicular DNA is poor in normal adult rats and in pubertal hypophysectomized animals, RTF3 does not modify the thymidine incorporation in both conditions. The reasons for this lack of effect are discussed. An experimental condition of spermatogonial regeneration is obtained by testicular irradiation. Inhibin preparation inhibits the regenerative DNA synthesis.

Aging↗
PubMed1978

Immunohistochemical demonstration of several tumour markers in neoplastic and preneoplastic states of the uterine mucosa.

Enzymic immunocytochemical demonstration of five tumour markers (CEA, AFP, kappa-casein, total HCG and its beta-subunit) was performed on various pathological states of the uterine mucosa. CEA was constantly found in areas of atypical hyperplasia and in more than 60% of cancers. kappa-Casein on the contrary was an unexpected but frequent finding in cystic hyperplasia. The other antigens were not, or exceptionally, detected in the tissues. We believe that CEA detection may be a very useful tool in delineating preneoplastic changes of the endometrium while the presence of kappa-casein "storing cells" might point to an unopposed estrogen action.

Carcinoembryonic Antigen↗
PubMed1978

[Organ cultures of mammalian testes. I. Adult mouse testis].

Organ cultures of adult normal mice testes have shown that the tubular structure is best preserved at 31 degrees C. With the simple anhormonal conditions we have used, the germ cells actively involved in meiosis disappear in ten days, following a stereotyped pattern. During the same time, Sertoli cells become enlarged, with a marked cytoplasmic vacuolisation. After 2 weeks in vitro, the seminiferous tubules are completely filled up with Sertoli cells and no more spermatocytes are recognized.

Animals↗
PubMed1978

[Cellular receptors of gonadotropic hormones. Immunocytochemical evidence in rat testicle].

We have performed topical applications of HCG and LH on fixed paraffin-embedded sections of adult rat testes. Thereafter, immuno-cytochemical demonstration of the peptides was carried on and demonstrated that HCG and LH specifically bound to the cell membrane of Leydig cells. This technique is highly reproductible provided that adequate fixation (with formalin containing fixatives) as been performed.

Animals↗
PubMed1978

[Fate of Leydig cells in testicular organ culture].

In organ culture experiments, the Leydig cells of mouse testes can still be recognized after 12 days at 31 degrees C. HCG binding sites are effectively demonstrated. Meanwhile, when HCG is added at the initiation of the cultures, it is selectively stored in the cytoplasm of the Leydig cells. These findings point to a fairly good survival of Leydig cells in our tissue culture system.

Animals↗
PubMed1977

Comparison of histological changes seen in placental tissue cultures and in placentae obtained after fetal death.

The histological changes in placental tissue cultures were observed and compared with those seen in placentae obtained after intrauterine fetal death. Early damage of the syncytiotrophoblast and improvement in the appearance of cytotrophoblast were seen in both cases. It is suggested that it is the cessation of the fetal circulation which causes these changes.

Culture Techniques↗
PubMed1977

[Effect of synthetic progestogens on endometrial cancer. Cytochemical criteria].

1) Well-differentiated carcinomas of the endometrium contain acid mucins with carboxyl or, more rarely, sulphated radicals. The latter are found in certain hypersecretory papillary tumours. 2) The neoplastic cells are surrounded by a border rich in acid muco-substances, including sialic acid. This pericellular film is unaffected by progestogens. 3) With progestogen therapy, there is a marked increase in the secretion of muco-substances in sensitive malignant cells. There is also more glycogen in the cytoplasm. No qualitative change in secretion may be demonstrated. 4) The intracytoplasmic accumulation of glycogen and mucopolysaccharide material is inversely proportional to the mitiotic index.

Cytoplasm↗
PubMed1975

Effect of protein hormones and steroids on tissue cultures of endometrial carcinoma.

Neoplastic tissue was obtained from 30 patients with endometrial carcinoma and the response of this tissue to various hormones was studied in vitro by histological methods and by measuring the incorporation of 3-H thymidine into DNA. Progesterone and synthetic progestogens had a consistently adverse effect on tissue survival in vitro while pregneolone had a beneficial effect.

Autoradiography↗