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Biomedical subjects

J Hustin

Publications and source records attributed to J Hustin.

At least 73 records · Page 4Linked to original sources
PubMed1984

Cellular proliferation in villi of normal and pathological pregnancies.

DNA and protein synthesis have been studied in placental villi from normal and pathological cases by in vitro incorporation of tritiated thymidine or tritiated proline and subsequent counting or autoradiography. It appeared that cytotrophoblastic DNA synthesis continued until term and that it was particularly important in preeclampsia cases and in cases of villous immaturity (rhesus sensitization). Protein synthesis was also increased in preeclampsia and seemed to be due to a very active cytotrophoblastic metabolism. The most interesting finding was that in preeclampsia cases, especially when intrauterine growth retardation was superimposed, villous capillary endothelial cell proliferation was as prominent as in cases where villous maturation was not achieved. Such results are highly suggestive of an important compensatory proliferative mechanism in the placentae of preeclamptic women.

Autoradiography↗
PubMed1984

[Are chorionic gonadotropin (HCG) and its alpha and beta subunits useful markers in non-trophoblastic tumors?].

HCG and its subunits alpha and beta are produced by trophoblastic cancers constituting an index of early detection and monitoring for these tumors. Unlike HCG-alpha, we can obtain specific HCG and HCG-beta assays with LH-neutralized antiserum. Many normal non-trophoblastic tissues exhibit a HCG-like immunoreactivity. All choriocarcinomatous testicular tumors produce HCG and HCG-beta. Half of all testicular teratomas produce HCG and its subunits while a third of all seminomas exhibit an HCG-like immunoreactivity, whether choriocarcinomatous component is present or not. Serum HCG levels are elevated in seminomas (5 to 22%) as well as teratomas '55 to 89%). Less than 15% of breast, digestive and lung cancers have increased serum levels of HCG and/or its 2 subunits. HCG is most often produced by undifferentiated lung cancers, hepatoblastomas and adrenal carcinomas. There is usually a parallel relation between these serum levels and the clinical evolution of the disease under chemotherapy. In breast cancer, these levels do not constitue a "prognosis index". HCG production by non-trophoblastic tumors can induce clinical symptoms such as precocious puberty and gynecomastia.

Breast Neoplasms↗
PubMed1983

[Sites of production of prolactin in the fetoplacental unit].

Immunocytochemical techniques can be used to demonstrate prolactin immunoreactivity in amniotic membranes, especially in the decidual cells, but also in primary trophoblastic cells embedded in the choriodecidual junction. While it is possible that some of the prolactin is synthetised in situ, part of it may also be bound to its specific receptors.

Decidua↗
PubMed1983

[Influence of ultrasonics on the fecundity of female rats].

We have studied the effects of ultrasound on the female rat fertility. When utilized at similar power than for human beings, the echography reduces significantly the number of foetus counted at the 15th day of the gestation. A direct action of the ultrasounds on the mechanisms of the ovulation is to be considered.

Animals↗
PubMed1983

[The placenta in trisomy in the last trimester of pregnancy].

18 cases of trisomies 13, 18, or 21 were diagnosed either by examining the caryotype or on clinical criteria. When the placenta was examined in 60% of cases there was a marked retardation of villous maturation with frequent persistence of embryonic forms of villi. The authors suggest that the failure of the villi to mature was often a reflection of pathology in the oocyte which started to show itself at the beginning of the pregnancy.

Chromosomes, Human, 13-15↗
PubMed1983

Maternal vascular lesions in pre-eclampsia and intrauterine growth retardation: light microscopy and immunofluorescence.

Placental bed biopsies were performed during caesarean section in a series of 137 patients. Analysis of the morphological findings confirms that vascular physiological changes were reduced in pre-eclampsia and in normotensive intrauterine growth retardation. In pre-eclampsia, acute atherosis in the decidual segments of uteroplacental arteries was a prominent feature. Intimal thickenings of the myometrial segments of the uteromaternal arteries were also noted. Normotensive intrauterine growth retardation cases were characterized by intimal thickenings of the myometrial segments of the uteroplacental arteries. Immunofluorescent investigations have demonstrated that the deep vascular stenoses were not associated with immunoglobulin deposition while in distal arterial segments displaying acute atherosis a positive immunofluorescence for IgG and fibrin and, more irregularly, for C'3 and IgM could be noted. These findings lead us to suggest that an immunological mechanism may be involved in the pathogenesis of acute atherosis.

Complement C3↗
PubMed1982

Organ cultures of mammalian testes. II. Meiotic chromosomes of adult mice in vitro.

Meiotic chromosomes of adult mice testes cultured in vitro have been studied. All meiotic stages are present and normal until the 8th day; suitable figures for chromosome studies may be obtained with routine cytogenetic methods. During the 9th and 10th day in vitro, the number of cells in meiotic progress falls abruptly. By the 14th day, only a few pachytene stages are still observed. This breakdown of meiosis, linked with the disappearance of the germ cell line, is not due to chromosome anomalies that could have arisen in the new environment. Labeling with tritiated thymidine suggests that spermatogonia do not enter meiotic division in vitro. Chromosomes observed with this technique belong to cells already involved in meiotic processes at time of the initiation of the culture.

Animals↗
PubMed1982

[Tumor markers].

Several substances, referred to as tumor markers, are associated with neoplasms development. The specificity of these cancer related substances or antigens depends on their nature (onco-fetal antigens, placental antigens) or on their concentration and physico-chemical forms (hormones, exocrine products, enzymes,...). On the basis of physico-chemical, immunochemical and biochemical analogies which exist between these tumor markers and substances normally found at particular times of life, a classification of these markers may be proposed. Tumor markers are almost constantly found within carcinoma cells by immunocytochemical techniques and are secreted by carcinoma explants in culture medium. On the hand, the release of tumor markers in biological fluids (blood, cerebrospinal fluid, urines,...) is less frequently detected by sensitive methods such as radioimmunoassay. Several factors are responsible for this discrepancy between the intra-tumoral presence of tumor markers and the lower incidence of their detection in biological fluids. These factors are discussed. These tumor markers have attracted considerable attention from pathologists and clinicians. Thus, detection of these substances, especially by immunocytochemical methods, may be related to a situation of neoplastic transformation and allow a functional classification superimposed to histological classification superimposed to histological classification of tumors. Moreover, ectopic production of hormones and/or neuromediators explains some clinical symptoms in cancer processes. Furthermore, products of normal cell activity at the origin of cancer (hormones, enzymes, exocrine products) when evidenced within the neoplastic cells or within serum might constitute a hormonal dependence index useful for therapeutical orientation. Finally, tumor marker levels are related to the local and systemic extension of the neoplasia and may be considered as valid index of prognosis. The determination of the levels of these tumor markers provides a quantitative criterion of the evolution of the neoplastic disorder and for following the efficacy or inefficacy of treatment.

Adrenocorticotropic Hormone↗
PubMed1982

[Neonatal autopsy and genetic counseling. 350 cases].

Congenital anomalies or malformations have now become the principal cause of neonatal deaths, as confirmed by the present study of 350 consecutive autopsies studied from 1977 to 1981. The overall incidence of congenital malformations for the whole period considered was of 36.8%. However, during this short four-year lapse of time, a steady increase from 26.8% in 1977 to 48.1% in 1981 could be noticed. This increase is concomitant to the decrease of the "classical" causes of neonatal deaths, namely prematurity sequellae and intrapartal death. Indeed, many different entities have been identified; it seems interesting to point out, however, the relatively great number of autosomal recessive diseases found in the present series. For a full genetic profit, neonatal autopsy should be complete and systematically followed by genetic counseling.

Abnormalities, Multiple↗
PubMed1981

Comparison of levels of cytosol estrogen receptors with "arterial" and "venous" concentrations of gonadic steroids in mammary tumors.

Plasma steroid concentrations measured in the post-menopausal women with breast cancer showed a close correlation between the various androgens. The post-menopausal women exhibited a correlation between estrogen receptors (ER) and "arterial" 17 alpha-hydroxyprogesterone levels in the breast. ER levels expressed as a function of plasma (arterial) steroid (P/17 P) molar concentration ratio were distributed within a hyperbola, suggesting the existence of a control mechanism for breast cell ER levels.

Aged↗
PubMed1979

Estrogen treatment of postmenopausal vaginal atrophy--a cytological assessment.

The following is an assessment of the maturation value (MV) based upon cytohormonal smears of postmenopausal or hysterectomized patients: (1) There is a negative correlation between the MV and the number of postmenopausal years. (2) There is a positive correlation between MV values higher than 60 and urinary excretion of estrogens. (3) The MV is ideally suited to demonstrate the beneficial effect of various estrogens on the vaginal mucosa. (4) Though its action is delayed, and necessitates a threshold dose of 33 micrograms/kg weight/wk, estriol might be considered as the drug of choice for a beneficial effect on the vaginal mucosa, because of its lack of endometrial action at this dosage.

Adult↗