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Biomedical subjects

J Huber

Publications and source records attributed to J Huber.

At least 235 records · Page 13Linked to original sources
PubMed1986

Immunohistology of the thymus in bone marrow transplant recipients.

The immunohistological findings in the thymus after bone marrow transplantation were studied in autopsy samples from 12 patients who had received allogeneic grafts as treatment for acute leukemia. The findings were compared with those in samples from normal individuals and from conventionally treated leukemic patients. The thymuses were hyperinvoluted in all grafted and nongrafted subjects. The remnants were composed of subcapsular and medullary epithelium which expressed the same phenotype as the normal thymus controls. Most samples also contained small remnants of cortical epithelium which lacked normal expression of HLA-DR antigens. The intraepithelial and stromal thymic lymphocytes were virtually all mature T cells, and no immature cortical thymocytes were seen. With the use of HLA-typing methods in 2 recipients of one-haplotype-matched grafts no donor cells could be identified in any of the thymic components. These findings provide no evidence for a functional role for the thymus in the differentiation of donor-derived precursor T cells, at least in the early period after transplantation.

Acute Disease↗
PubMed1985

Glucocorticoids regulate the transcription of glycerol phosphate dehydrogenase in cultured glial cells.

Utilizing a glycerol phosphate dehydrogenase cDNA clone from the rat glioma C6 cell line, we report that the glucocorticoid regulation of glycerol phosphate dehydrogenase gene expression in glial cells occurs at the transcriptional level. Furthermore, our transcription results in vitro, as well as Northern blot analysis, show that the short-chain fatty acid, sodium butyrate, totally blocks this hydrocortisone-induced transcription of glycerol phosphate dehydrogenase, demonstrating that the site of action of this fatty acid resides in the cell nucleus. The C6 glycerol phosphate dehydrogenase cDNA clone (pGPDH-1,1800 base pairs) used in these studies was selected from a C6 library generated from polysomal poly(A)+ RNA. pGPDH-1 hybridized to a 4.7-kilobase RNA from rat muscle and brain, mouse liver, and C6 cells; this mRNA is at least four times larger than the required coding sequence for glycerol phosphate dehydrogenase. Northern blot analysis of developing rat brain reveals a striking increase in glycerol phosphate dehydrogenase transcripts during the period most associated with peak myelination.

Animals↗
PubMed1985

[Perinatal mortality of children at the Vienna University Gynecologic Clinic between 1981 and 1983].

All cases (77) of perinatal death between 1981 to 1983 are described and discussed. 15 pregnant patients arrived for delivery without any examination during the pregnancy. In 11 cases perinatal death happened in spite of regular examinations. The distance between the last examination and the date of perinatal death was in every case more than 4 weeks. 15 malformations could be registrated. 5 gravidae delivered at home and there the fetus died. A mistake in our management could be found 3 times.

Adolescent↗
PubMed1985

[Micromanipulatory sperm injection--a new method in the treatment of infertile males].

Three couples were selected for this preliminary sperm injection study with their consent, since in all three cases the zona free hamster egg penetration test was negative and in one couple previous attempts of in vitro fertilization were unsuccessful. Of 22 eggs, recovered from three patients, 9 from each patient 3 were injected with sperm, while the others were inseminated by the standard IVF-protocol. Before manipulating human eggs extended experiments were performed with animals eggs, so decreasing the rate of damage to under 10%. Additionally we optimized the method by using an individual adapted equipment. Manipulation was performed by penetration of the zona pellucida with a 5 to 7 mu thick injection pipet into the perivitilline space so damaging of the egg cytoplasm was avoided. This special technique for sperm injection was not used till now in this indication. From the nine eggs used in this experiment one egg developed a pronucleus and one egg developed to a four-cell stage. We could not achieve a pregnancy.

Embryo Transfer↗
PubMed1985

Matrix depletion of young and old human articular cartilage by cultured autologous synovium fragments: a chondrocyte-independent effect.

Human articular cartilage of different ages was cultured for 8 days and proteoglycan (PG) release into the medium was measured. Retinol and synovial co-culture increased the PG release of cartilage of all ages. The effect of retinol was dose-dependent. Synovium increased also the PG release of dead cartilage, whereas retinol did not. The increased PG release by synovial co-culture is therefore mainly the result of synovial enzymes acting directly on the matrix rather than of a factor inducing chondrocyte-mediated breakdown.

Age Factors↗
PubMed1985

The thymus in "bare lymphocyte" syndrome: significance of expression of major histocompatibility complex antigens on thymic epithelial cells in intrathymic T-cell maturation.

Thymic biopsies from two patients with combined immunodeficiency and defective expression of HLA class I and class II antigens on blood mononuclear cells ("bare lymphocyte" syndrome) were investigated. This made possible an evaluation of the significance of HLA antigen expression in a detailed (immuno)histologic study. Both thymuses showed a normal lobular architecture with distinct cortex-medulla areas, well-differentiated epithelium, including ultrastructurally defined subtypes, and Hassall's corpuscles. Normal numbers of lymphoid cells were present and normal T-cell phenotype was found. Using anti-HLA-A,B,C antisera, confluent staining of the medulla (stroma and lymphocytes) was observed. One of the thymuses was found to be negative for HLA class II antigen expression: the other revealed only HLA-DR positivity of nonlymphoid cells in the medulla. These cells were not of epithelial nature as judged from double staining with anti-keratin antibody. There was no expression of HLA-DC/DS. These observations differ from findings in the normal thymus, wherein epithelial cells in the cortex carry HLA class I and class II antigens, and epithelial cells in the medulla express HLA class I, and for a minor part class II antigens. The results indicate a normal sequential acquisition of T-cell differentiation antigens in the thymus of both cases. It is suggested that the expression of HLA class I and class II antigens on epithelial cells in the normal thymus cortex does not play a significant role in the sequential acquisition of differentiation antigens on T lymphocytes.

Antigens, Surface↗
PubMed1985

Effects of different-shaped tissue expanders on transluminal pressure, oxygen tension, histopathologic changes, and skin expansion in pigs.

This study, performed on domestic pigs, was designed to show whether the shape and size of a tissue expander influences the amount of surface area increase. When a defect is irregular, a larger custom-fitted implant is more efficient than several smaller expanders. Oval implants gained 5 percent more skin using 50 percent less time and almost 20 percent less intraluminal pressure, while U-shaped implants yield 2.2 percent more skin in 10 percent less time with 35 to 50 percent less pressure when compared with several smaller implants covering the same surface area. Transcutaneous TpO2 measurements and histologic evaluation revealed no significant difference between all implant shapes. These results show that less time, pressure, and volume are needed in a larger implant to gain the same surface area as would be attained from several smaller implants, thus sparing the patient more discomfort, trauma, and risk of infection by reducing the number of filling domes and hence the number of fillings.

Animals↗
PubMed1985

Medroxyprogesterone acetate therapy in hirsutism.

In a pilot study, 26 hirsute females were treated with medroxyprogesterone acetate (MPA). We used an ointment containing 0.2% MPA in 13 patients, subcutaneous injection of MPA into the hairy areas of the face in five patients and intramuscular injection of low doses of MPA in 13 patients, each for an average duration of 16 weeks. The best clinical result was achieved by subcutaneous injection, the next most successful was the intramuscular injection, and the least successful was the topical application. Hair diameter measurements, carried out in 12 patients, were found to be reduced by 33% on average. Serum androgen levels, measured monthly, remained unaffected by topical treatment, but were decreased by intralesional and intramuscular injection. The clinical improvement with topical therapy despite the lack of effect on serum androgen levels supports the idea that MPA works partly at the cellular level. The intralesional and systemic injection of MPA may exert an effect both by systemic androgen suppression and a local action.

Administration, Topical↗
PubMed1985

Steroid hormone levels in serum and skin receptor concentrations in hirsutism.

In 36 women androgen (AR), estrogen (ER) and progesterone (PgR) receptor were determined by dextran coated charcoal assay and subsequent Scatchard plot analysis in skin slices obtained from hirsute areas under local anesthesia. The biopsies were performed between day 18-21 of the menstrual cycle. Concomitantly venous blood was sampled in these and additional 14 patients for radioimmunoassay estimation of serum levels of testosterone (T), dehydroepiandrosterone sulfate (DHEA-S), androstenedione (A), estradiol (E2) and prolactin (PRL). However, it should be noted that all receptors and hormones were not examined in all patients. The incidence of ER-positive patients was smaller (i.e. 17%) than previous findings in acne and normal skin, while 26% of the examined patients were AR-positive. This result was similar to findings in acne, whereas the receptor levels are three times higher than in acne lesions. This suggests a greater androgen sensitivity of hirsute areas compared to acne lesions. Two out of 10 examined patients were PgR-positive. T was elevated in 63%, DHEA-S in 50% and A in 37% of examined patients. Lack of correlation between hormone serum levels and corresponding receptors concentration indicates a direct involvement of hormone action at the cellular level. The data combine to suggest that greater target organ sensitivity was responsible for hirsutism in those patients with normal androgen serum levels, while the absence of receptors in skin samples may characterize patients with idiopathic hirsutism.

Adult↗
PubMed1984

[Serum endorphins in the perinatal period].

In 22 pregnant women beta-ELIR and N-terminal-beta-Lipotropin serum levels were determined by radioimmunoassay (New England Nuclear) in the last three weeks of pregnancy, at the beginning and at the end of delivery and during 48 hours after delivery. The difference between the results of the two examinations (beta-ELIR and N-terminal-beta-lipotropin) represents with close approximation the beta-endorphin concentration. In 12 patients the beta-endorphin level during delivery was significantly higher than during late pregnancy; in three cases an extremely high beta-endorphin concentration was found in pregnant women with a dystrophic infant. Therefore, beta-endorphin is discussed as an indicator for foetal distress.

Endorphins↗
PubMed1984

Dento-alveolar abnormalities in oculomandibulodyscephaly (Hallermann-Streiff syndrome).

Oculomandibulodyscephaly (OMD) is characterized by anomalies of skull, mandible, skin and eyes and also by a proportionate nanism. Dental abnormalities are often observed but poorly defined. Examination of a 2-month-old infant that suffered from OMD, having died from an unrelated cause revealed premature eruption of several deciduous teeth, crowding of unerupted deciduous teeth and agenesis of all permanent teeth excepting the first permanent molars.

Hallermann's Syndrome↗