Biomedical subjects

J Huang

Publications and source records attributed to J Huang.

At least 307 records · Page 17Linked to original sources

PubMed2000

Pulmonary embolism associated with nephrotic syndrome in children: a preliminary report of 8 cases.

OBJECTIVE: To investigate the incidence, clinical features of pulmonary embolism (PE) secondary to nephrotic syndrome(NS) in Chinese children. METHODS: Pulmonary perfusion imaging (PPI) and chest radiography was performed on 20 NS patients with elevated plasma D-dimer. The patients with PE were given anticoagulative therapy with heparin and persantine. RESULTS: Among 20 NS patients, eight cases, aged from 5 to 12 years, 6 boys and 2 girls, were found to have PE. Of them 5 were primary NS and 3 were secondary NS. PPI showed an involvement of 1 pulmonary segment in 3 cases, of 2 pulmonary segments in 2, of 3 pulmonary segments in 2, and of 6 pulmonary segments in 1. Most patients did not present clinical signs and symptoms. Two weeks after the administration of intravenous heparin for anticoagulative therapy, 6 out of 8 cases showed a recovery or an improvement. CONCLUSION: The results of this study suggest that PE be not a rare complication in NS children. Most patients had no obvious clinical manifestation and their prognose was favorable if diagnosed and treated in time.

Adolescent↗

PubMed2000

Platelet-derived growth factor and basic fibroblast growth factor immunolocalized in proliferative retinal diseases.

OBJECTIVE: To study the role of platelet-derived growth factor (PDGF) and basic fibroblast growth factor (bFGF) in the development of proliferative vitreoretinopathy (PVR). METHODS: Immunohistochemical localization was performed on frozen sections of the epiretinal or subretinal membranes from 21 PVR patients using! monoclonal antibodies against PDGF and bFGF and the streptavidin peroxidase system. RESULTS: PDGF was expressed in 15 of 21 specimens (71.4%), and bFGF was expressed in 14 specimens (66.7%). Different cell density was seen in different types of epiretinal membranes. The cell density of vascular membranes induced by PDR and Eales disease was the highest (> or = 61 cells/field, x 1000); nonvascular membranes induced by PVR or traumatic PVR had moderate cell density (31-60 cells/field); and the macular membrane had the lowest cell density (< or = 30 cells/field). CONCLUSION: The presents of PDGF and bFGF in PVR tissues suggest that PDGF and bFGF may play an important role in the development of PVR.

Epiretinal Membrane↗

PubMed2000

Molecular and immunohistochemical study of the inactivation of the p16 gene in primary hepatocellular carcinoma.

OBJECTIVE: To determine whether p16 gene is involved in the genesis of primary hepatocellular carcinoma (HCC). METHODS: Twenty-five HCC tumor samples with corresponding non-tumor liver tissue specimens were examined for p16 gene alterations. The identification of deletion of exon 1 and exon 2 in p16 gene was performed using comparative multiplex polymerase chain reaction (PCR) analysis. The point mutation of exon 2 in p16 gene was investigated by single strand conformational polymorphism (SSCP) analysis, and the status of p16 gene methylation was screened using a PCR based methylation analysis. 35 parafin-embedded specimens of HCC with corresponding non-tumor liver tissues, including the 25 cases described above for screening p16 gene alterations, were investigated for p16 protein expression using immunohistochemical analysis. RESULTS: Among 25 cases, 2 homozygous deletions and 1 hemizygous deletion were found in HCC samples. No point mutation was identified in the remaining 22 tumor samples without p16 gene deletions. Hypermethylation was detected in 24% (6/25) of tumor samples. However, the corresponding non-tumor liver tissue specimens were always unmethylated at the p16 locus. Loss of p16 protein expression occurred in 16 of 35 (45.7%) tumor samples, and all the non-tumor liver tissue specimens showed positive p16 staining. For the 25 cases examined for p16 gene alterations, the loss of p16 protein expression was observed in all tumors with p16 gene alterations and also in 3 tumors without p16 gene alterations. CONCLUSION: Inactivation of the p16 gene may play an important role in the genesis of primary hepatocellular carcinoma.

Carcinoma, Hepatocellular↗

PubMed2000

Effect of preoperative transcatheter arterial chemoembolization on tumor cell activity in hepatocellular carcinoma.

OBJECTIVE: To evaluate the role of preoperative transcatheter arterial chemoembolization (TACE) as a palliative approach in hepatocellular carcinoma (HCC). METHODS: From January 1992 to December 1998, 279 patients with HCC underwent curative liver resection. One to five courses of TACE prior to liver resection were performed in 117 patients (TACE group), while the other 162 patients received only liver resection (control group). All 279 specimens of resected tumors were submitted to the following assessments: PCNA and expression of P53 protein. All specimens from the TACE group were examined for downstaging or necrosis of tumors. RESULTS: In the TACE group, gross inspection revealed downstaging or necrosis of tumor in all cases. Total necrosis (100%) of tumor was observed in 11.1% of 117 patients, > 90% but incomplete necrosis in 15.4%, 50%-90% necrosis in 46.2% and < 50% necrosis in 27.3%. Microscopically, extensive and homogenous coagulative necrosis was observed. Viable cancer cells were also present within and outside the tumor capsule in 111 cases. In the remaining 6 cases, the tumor necrosed completely. In control group, necrosis was observed in 8.0% of 162 cases and reduction of tumor size was < 20%. Microscopically, viable HCC cells were noted in all cases. There was no statistical difference in expression of P53 protein between the TACE and control group. High labeling index of PCNA was significantly higher in the TACE group. CONCLUSIONS: TACE has a marked antitumor effect resulting in various degree of tumor necrosis, but only a small proportion of tumors show complete necrosis. Since the residual tumor cells following preoperative TACE may have more aggressive behavior, we conclude that sequential liver resection is the preferred therapy whenever feasible and preoperative TACE should be avoided in resectable HCC.

Adult↗

PubMed2000

[Expression of S-100 proteins and intermediate filament proteins in pleomorphic adenoma].

OBJECTIVE: To observe immunohistochemical expression of S-100A1, S-100A2, S-100A4, S-100A6, S-100B, K8.12, KL1, Vimentin, GFAP and NSE in pleomorphic adenoma of salivary gland, and to evaluate the differential localization of S-100 proteins and biological behaviour of neoplastic myoepithelial cells. METHODS: 23 cases of normal salivary gland and 60 cases of pleomorphic adenoma were embedded, in paraffin and were routinely diagnosed on the basis of hematoxylin and eosin-stained sections. Serial sections at 4 microns from the paraffin embedded blocks were used for the immunohistochemical studies. RESULTS: Normal salivary glands had positive immunoreactivity for S-100A families, K8.12 and KL1 in the ductal cells, while S100B, GFAP and NSE were observed in peripheral nerve fibers innervating the gland. In pleomorphic adenomas, luminal tumor cells in duct-like structures had positive immunoreactivity for S-100A subfamilies and keratin. Nonluminal tumor cells had positive immunoreactivity for S-100B, as well as Vimentin, keratins detected by monoclonal K8.12 and KL1, GFAP and NSE. CONCLUSIONS: These findings may suggest that the neoplastic myoepithelial cells contain Ca2+ binding proteins which may have a role in the regulation of calcium ions or calcium signaling mechanisms in the modulation of extracellular matrix deposition in pleomorphic adenoma which may in turn affect the extracellular matrix synthesis as well as histomorphology of the tumor.

Adenoma, Pleomorphic↗

PubMed2000

[Clinical study on the expression level of lymphocyte function-related antigen of breast cancer patients].

OBJECTIVE: To explore the protein expression level of the lymphocyte function-related antigen CD11a, CD18, CD54 of breast cancer patients' blood, cancer tissue and drainage lymph node, and the effect of chemotherapy as well as Yiqi Yangxue Granule on its expression level. METHODS: The immunohistochemical assay and flow cytometry were used to determine the change of the protein expression level of CD11a, CD18, CD54 on the lymphocyte of blood, cancer tissue and lymph node between before and after chemotherapy and chemotherapy plus Yiqi Yangxue Granule in 72 patients suffering from breast cancer. RESULTS: In breast cancer patients, the level of CD54 was increased more obviously than that of control group, the CD11a, CD18, CD54 of lymphocytes in cancer tissue increased remarkably, while the CD54 expression positive rate reached 100%, the expression level of CD11a, CD18, CD54 which increased significantly in negative lymph node obviously higher than that of positive lymph node. CONCLUSION: The level of adherence cell was markedly abnormal in breast cancer patients, whose immune adherence function disturbed. Although chemotherapy kills the tumor cells, it damages the immune adherence function, Yiqi Yangxue Granule could improve the immune function and enhance the anti-cancer effect of patients.

Adult↗

PubMed2000

[Study on relationship of parameters of nitric oxide, endothelin and blood stasis syndrome in traditional Chinese medicine in acute cerebral infarction].

OBJECTIVE: To explore the relationship of parameters of nitric oxide (NO), endothelin (ET) and blood stasis syndrome (BSS) of traditional Chinese medicine (TCM) in acute cerebral infarction (ACI). METHODS: Seventy-three patients of ACI (ACI group) were divided into two groups by the score of BSS: BSS group (included 18 cases of serious BSS, 19 cases of moderate BSS, and 21 cases of mild BSS); and non-BSS group (15 cases); while 30 healthy subjects were taken as the control group, their NO and ET were determined. RESULTS: Compared with the control group, the level of ET and ratio of ET/NO raised significantly in the ACI group and the BSS group (P < 0.01). The levels of ET and ratio of ET/NO of mild, moderate and serious BSS increased in order, but only the serious BSS in comparing with mild, moderate group of BSS had significant difference (P < 0.01). The NO levels of ACI group, non-BSS group in comparing with healthy control, there was significant decrease (P < 0.05, P < 0.01). The NO concentration among the mild, moderate and serious group of BSS had insignificant difference (P > 0.05). CONCLUSION: NO and ET participates the formation and development of ACI, owing to the disturbed NO and ET secretion by vascular, nerve cells etc., these might be the pathologic basis of BSS occurrence.

Acute Disease↗

PubMed2000

[Relationship between serum insulin, C-peptide in hypertension and syndrome differentiation-typing in TCM].

OBJECTIVE: To study the relationship between insulin-resistance (IR), hyperinsulinemia and TCM Syndrome Differentiation-typing. METHODS: The serum insulin, C-peptide level of the four Syndrome-types of hypertension (30 cases each type) and the control group (30 cases) were determined. RESULTS: The serum insulin level in hypertension patients were significantly higher than that of control group, and there were obvious difference among the four types of Syndrome. The following order was: The abundant phlegm-dampness type > exuberant Liver-Fire type > both Yin-Yang deficiency type > Yin deficiency and Yang-Excess type > control type. CONCLUSIONS: The Excess Syndrome was severe and deficiency Syndrome was mild in hyperinsulinemia. The pattern of change was in accordance with etiology and pathogenesis of TCM. It has the guiding significance to the clinical practice and research of TCM and integrated TCM-WM.

Aged↗

PubMed2000

[The treatment of premature arrest of growth plate with a novel engineered growth plate: experimental studies].

OBJECTIVES: To observe the role of the engineered growth plate (EGP) in the treatment of premature arrest of growth plate and to establish a novel treatment method for the premature arrest of the growth plate. METHODS: The engineered growth plates were cultured for the first time by using polylactic acid (PLA) as cell scaffold seeded with growth plate chondrocytes and they were implanted into the medial proximal defects of growth plates of New Zealand rabbit tibia. The degree of deformity of the tibia was evaluated by X-ray and the expression of collagen II mRNA of regenerating growth plate was detected by in situ hybridization technique. RESULTS: Little deformity appeared in the EGP group 8 weeks after operation. Some deformity was seen in the EGP group 16 weeks after operation, whereas it's degree was much less than that of the control group (P = 0.0001). The degree of the angular deformity of the EGP precultured with bFGF and TGF-beta group was less than that of the EGP group (P < 0.05). The cells in the regenerating growth plate arranged in column and were stained blue by in situ hybridization technique. CONCLUSION: The EGP can prevent the formation of bony bridge and restore the growth of the damaged growth plate.

Animals↗

PubMed2000

[The recognition of the epidemic area of tsutsugamushi disease on Nan Peng Lie Island in China and the strategy of prevention].

OBJECTIVE: To investigate the region for the epidemic area of tsutsugamushi disease. METHODS: Epidemiological studies, Orientia tsutsugamushi isolation, and preventive measures were used. RESULTS: The region belonged to epidemic area of south subtropical zone. The main host was found Rattus norvegicu. The main biological vector was Leptotrombidium deliens. The seasonal trends of the quantity of Rattus norvegicu and Leptotrombidium deliens were consistent with the incidence change of the disease in the region. Orientia tsutsugamushi has been isolated from Rattus norvegicu and Leptotrombidium deliens. Data showed that Orientia tsutsugamushi isolated strains mainly belongs to Karp. Sero-epidemiological data showed that antibodies of Orientia tsutsugamushi are 100.0% positive in the residents of the region and 4.0% in army men. After taking comprehensive preventive measures, the incidence was descending. CONCLUSION: Nan Peng Lie island was proved an epidemic area of tsutsugamushi disease.

Animals↗

PubMed2000

[Increased expression of peritoneal macrophage CD14 in severely burned mice].

OBJECTIVE: To observe the dynamic changes in peritoneal macrophage (Mphi) CD14 expression in severely burned mice. METHODS: The mice, being inflicted with 20% TBSA III degree burn, were randomly divided into two groups: Group A: The mice in this group received fluid resuscitation instantaneously after burn. Group B: The mice in this group received delayed fluid resuscitation at 6hr postburn. The Mphis at different postburn time points were obtained in two groups for the detection of their expression of CD14 and CD14 mRNA with immunohistochemical and RT -- PCR analysis, and also for the determination with ELISA of the changes in TNF and IL -- 6 levels in the supernatant of their culture. RESULTS: The expression of CD14 and the levels of TNF and IL -- 6 increased obviously in both groups from 1 to 24 hrs postburn. CONCLUSION: Burn injury would induce the increase of the expression of Mphi CD14 mRNA in mice. As a result, the Mphi became more readily to be activated by low levels of LPS. The activated Mphi might play an important role in the development of postburn SIRS.

Animals↗

PubMed2000

[Tangent time constant and wave-speed mechanism of F-V curve configuration in smokers and clinical significance].

This study was conducted to explore the mechanical mechanism of the formation of F-V curve configuration with tangent time constant (tau 1) and wave-speed theory for smokers. F-V curves were measured on 22 passive smokers and 94 active ones. FVC, V75, V50 and V25 were obtained, and tangent time constants at high, mid, low lung volumes, tau t75, tau t50 and tau t25 were calculated, and analysed with wave-speed theory. The results showed that the chief changes taking place only in heavy smokers and convex type of F-V curve were: decreased V50 and V25; tau t25 > or = 1s; tau t75-tau t50 < tau t25 in "trumpet fashion" change; and tau t75 < tau t50 < tau t25 in "tower fashion" change. Then, tau t25 > or = 1s, tau t75-tau t50 < tau t25 and tau t75 < tau t50 < tau t25 were assigned as three tau t characters of smoking effect. They led to an increased convexity of the convex type of F-V curve, and reflected the decrease of small airway functions. Similar to the decrease of V50 and V25, they have important significance in assessing small airway functions and thus merit clinical use.

Adult↗

PubMed2000

[The relationship of brainstem auditory evoked potential to cerebral blood flow volume in with vertebrobasilar transient ischemic vertigo].

This study was aimed at the relationship of brain-stem auditory evoked potential(BAEP) with cerebral blood flow(CBF) volume and vascular pathological changes in patients with vertebro-basilar transient ischemic vertigo (VBTIV). 65 patients were examined by magnetic resonance angiography(MRA), transcranial Dopplar(TCD) and BAEP; 26 controls were examined by MRA and TCD. In the patient group, MRA showed that vascular pathological changes were obvious in 50 patients, and obscure or absent in 15 patients. The CBF volume [112.3-278.9 ml/min (2s)] of control group was higher than that (48.0-262.0 ml/min) of the patients group (t = 2.43, P < 0.01) in which 15 patients had low CBF volume and 50 patients had normal CBF volume. The BAEP of 47(72.3%) patients was abnormal. Out of 15 patients with low CBF volume, 14(93.3%) had abnormal BAEP, but out of 50 patients with normal CBF volume, only 33(66%) patients had abnormal BAED (chi 2 = 4.34, P < 0.05). In the 50 patients with obscure obvious vascular pathological changes, 40(80%) patients had abnormal BAEP, but in the 15 patients with obscure or without the changes, only 7(43.3%) patients had abnormal BAEP (chi 2 = 4.86, P < 0.05). These results suggested that there might be a close relationship of BAEP with CBF volume and vascular pathological changes.

Adult↗

PubMed2000

[Fasting serum glucose and its related indexes in 1477 middle aged and elderly people].

In order to study the relationship between higher fasting serum glucose (FSG) and other related indexes in middle aged and elderly people, FSG, triglyceride, cholesterol, urate and insulin of serum, blood pressure and body mass index(BMI) of 1477 men and women over 40 years of age were analyzed. The results showed that the level of FSG rised with the increase of age. The relations between FSG and cholesterol, urate, blood pressure and BMI were significant. The levels of cholesterol, urate, blood pressure and BMI in the abnormal FSG group were higher than those in the normal FSG group. There were positive relationships between FSG and cholesterol, urate, blood pressure, BMI, age, waist and buttock circumferences. The factors related to FSG were age, BMI, cholesterol and diastolic pressure by stepwise regression.

Age Factors↗

PubMed2000

[Column efficiency prediction of two dimensional chromatography by artificial neural network].

This paper used artificial neural network (ANN) modeling method to study the relationship between the column efficiency and the operating conditions. This method solved the problem that it is not easy to establish a quantitative model between the column efficiency and its main effecting factors using those traditional modeling methods, as the relationship is usually quite complex and non-linear in fact. The varied-pace BP (back-propagation) learning algorithm was adopted, and a three-layer weight-connected ANN model for a typical dual column system was established. The effective plate number representing the column efficiency acted as the output vectors, while the temperature of the pre-column, the temperature of the main column, the pressure difference between the columns and the vent rate acted as the input vectors. Then the model acquired was used to predict column efficiency (characterized by "effective plate number") under different operating conditions. The results showed that the model predicting value was in consistent with the value found. This work proved that ANN modeling method was suitable for the study on the relationship between the column efficiency of two-dimensional column chromatography system and the operating conditions.

Algorithms↗

PubMed2000

[Determination of vitamin D2 included with beta-cyclodextrin complex in "longmu zhuanggu chongji" by HPLC].

In order to enhance the stability of vitamin D2(VD2), the inclusion complex of VD2 with beta-cyclodextrin(beta-CD) was added in "Longmu Zhuanggu Chongji", a medicine of children for the prevention and cure of rickets. The VD2 in beta-CD-VD2 inclusion complex was satisfactorily determined by both reversed-phase high performance liquid chromatography(HPLC) and ultraviolet spectrometry(UV). The experimental results from UV were in well consistent with those from HPLC. The accuracy and recovery of HPLC measurement were analyzed, and their relative standard deviation(RSD) were given to be 0.63% and 1.54%, indicating the method is quite correct and reliable, and the linear regression equation is reasonable. The "Longmu Zhuanggu Chongji" containing beta-CD-VD2 inclusion complexes was saponified by a mixture of KOH, vitamin C (VC) and alcohol-water(1:2, V/V) at 70 degrees C, then extracted with petroleum ether. An accurate content of the VD2 product was obtained by HPLC. A simple and rapid HPLC method, which can detect VD2 in the inclusion complex and in "Longmu Zhuanggu Chongji", has been established in this work.

Chromatography, High Pressure Liquid↗

PubMed2000

[Detection of distortion-product otoacoustic emissions in well-drilling workers].

OBJECTIVE: To understand the value of using distortion product of otoacoustic emissions(DPOAE) in monitoring noise-induced hearing-loss. METHOD: The amplitude and the positive rates of DPOAE were detected in 102 well-drilling workers (195 ears). RESULT: The amplitude and the positive rates of DPOAE declined significantly in noise groups against control. With the increase of working-age the positive rates of DPOAE was decrease. The DPOAE amplitudes and the positive rates of noise group with normal pure tone threshold were obvious lower than that in controls. There were negative relationship between pure tone threshold in high-frequency and the positive rates. CONCLUSION: The DPOAE can reflect the cochlea's function of labors working in noise condition well. Contrast with the pure tone audiometry, DPOAE was more sensitive and more objective.

Adolescent↗

PubMed2000

[A study on the inactivation of p16 genes and the expression of P16 protein in primary hepatocellular carcinomas].

The aim of the present study was to determine whether p16 gene is involved in the genesis of primary hepatocellular carcinoma (HCC). Twenty-five primary HCC tumor and corresponding non-tumor liver tissue specimens were examined for P16 gene alterations. The identification of deletion of p16 gene exon 1 and exon 2 was performed using comparative multiplex polymerase chain reaction (PCR) analysis. The point mutation of p16 gene exon 2 was investigated by single strand conformational polymorphism(SSCP) analysis, and the status of p16 gene methylation was screened using PCR-based methylation analysis. Moreover, 35 parafin specimens of primary HCCs with corresponding non-tumor liver tissues, including the 25 cases described above for screening of p16 gene alterations, were investigated also for P16 protein expression by using immunohistochemical analysis. The results of comparative multiplex PCR analysis showed that 12% (3/25) p16 gene deletions, including homozygous deletions in 2 tumors and hemizygous deletion in 1 tumor, were found in the primary HCCs samples. No point mutation was identified in the remaining 22 tumor samples without p16 gene deletions by using SSCP analysis. Hypermethylation was detected in 24% (6/25) of tumor samples by PCR-based analysis. However the corresponding non-tumor liver tissue specimens were always unmethylated at p16 locus. Loss of P16 protein expression, detected by immunohistochemistry, occurred in 16 of 35 (45.7%) tumor samples, whereas all the non-tumor liver tissue specimens showed positive p16 staining. These results indicate that inactivation of p16 gene is relevant to the genesis of HCC.

Carcinoma, Hepatocellular↗